scholarly journals ISOLATION AND MORPHOLOGICALLY IDENTIFICATION OF ASPERGILLUS FLAVUS INCIDENCES FROM MAIZE SEEDS IN ABUJA, NIGERIA

2021 ◽  
Vol 4 (2) ◽  
pp. 5-8
Author(s):  
Hajara Oyiza Yusuf ◽  
Joshua Olu ◽  
A.J. Alu ◽  
T.S. Anjorin
Keyword(s):  
Experimental Design ◽  
Aspergillus Flavus ◽  
Morphological Characteristics ◽  
Potato Dextrose Agar ◽  
Fungal Isolate ◽  
Maize Seed ◽  
Experimental Field ◽  
Maize Seeds ◽  
Randomized Design ◽  
High Incidence

Food safety and security well implemented could help in making more crops available for consumption. Maize seed is a crop well known to be attacked by fungi such as Aspergillus flavus and reduce its nutrients. This study intends to isolate and morphologically identify the Aspergillus flavus from maize seeds from Abuja, Nigeria. The experimental design was complete randomized design involving untreated yellow (Y) and white (W) maize seeds from7 locations in Abuja, Nigeria. Pure culture of fungal isolate was prepared using Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Isolates obtained were characterized and identified on the basis of their colonial and morphological characteristics which include macroscopic and microscopic examinations. All maize seeds from the Abaji had no fungi incidences in both SDA and PDA, while all maize seeds from the experimental field show fungi incidences in both SDA and PDA. On SDA, the maize color yellow and white had F (2, 6) static values of 7.083 and 0.212 at p=0.129 and 0.941 respectively. For the PDA, white maize seeds and yellow maize seeds from all the locations had F (2, 6) static values of 0.377 and 0.521 at p=0.850 and 0.773. Aspergillus flavus was isolated in this study. The maize seeds from Kuje district and the experimental field 2 show a high incidence records. All growth of the Aspergillus in the two media used were not significant at p>0.05.

scholarly journals Comparative Study on the Incidence of Aspergillus flavus in Farmer’s Field and Stored Maize (Zea mays) Seed in Northern Region of Ghana

2018 ◽  
pp. 1-7
Author(s):  
Elias Nortaa Kunedeb Sowley ◽  
Frederick Kankam ◽  
Edmond Tawiah
Keyword(s):  
Zea Mays ◽  
Aspergillus Flavus ◽  
Northern Region ◽  
Aflatoxin Contamination ◽  
Maize Seed ◽  
Fusarium Spp ◽  
Maize Seeds ◽  
Tamale Metropolis ◽  
Total Fungi ◽  
Storage Structures

Aspergillus flavus is predominant among other fungi species responsible for aflatoxin contamination of crops on the field and in storage. A study was conducted to determine the incidence of Aspergillus flavus in maize seeds from various sources. Sixty (60) maize seed samples were collected from farmers and marketers from different storage structures within Tamale metropolis, Savelugu and Tolon districts,northern region of Ghana. Three different fungi species were isolated from the maize samples. These were Aspergillus flavus, Aspergillus niger and Fusarium spp. Total fungi species differed significantly. Tamale metropolis recorded the least occurrence of fungi species. Aspergillus flavus recorded varied occurrences across all three districts with the highest incidence recorded in Savelugu (42%). Aspergillus flavus was found predominant in maize from the markets than maize from the farmers. The incidences of Aspergillus flavus varied with the method of storage. Storage using cocoa sack recorded the highest incidence (69%), with the lowest (13%) in hanging shed. Aspergillus flavus was however found to be associated with maize from all three districts studied. Proper drying and use of proper storage structures are recommended as a way of combating the high incidences of Aspergillus flavus.

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aromatase Inhibitor ◽  
Fish Larvae ◽  
Clarias Gariepinus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

scholarly journals Outbreak of Leaf Spot and Fruit Rot in Florida Strawberry Caused by Neopestalotiopsis spp.

Plant Disease ◽  
2021 ◽  
pp. PDIS-06-20-1290
Author(s):  
Juliana S. Baggio ◽  
Bruna B. Forcelini ◽  
Nan-Yi Wang ◽  
Rafaela G. Ruschel ◽  
James C. Mertely ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Spore Production ◽  
Potato Dextrose Agar ◽  
Fruit Rot ◽  
Low Genetic Diversity ◽  
Pathogenicity Tests ◽  
A New Species

Pestalotiopsis-like species have been reported affecting strawberry worldwide. Recently, severe and unprecedented outbreaks have been reported in Florida commercial fields where leaf, fruit, petiole, crown, and root symptoms were observed, and yield was severely affected. The taxonomic status of the fungus is confusing because it has gone through multiple reclassifications over the years. Morphological characteristics, phylogenetic analyses, and pathogenicity tests were evaluated for strawberry isolates recovered from diseased plants in Florida. Phylogenetic analyses derived from the combined internal transcribed spacer, β-tub, and tef1 regions demonstrated that although there was low genetic diversity among the strawberry isolates, there was a clear separation of the isolates in two groups. The first group included isolates recovered over a period of several years, which was identified as Neopestalotiopsis rosae. Most isolates recovered during the recent outbreaks were genetically different and may belong to a new species. On potato dextrose agar, both groups produced white, circular, and cottony colonies. From the bottom, colonies were white to pale yellow for Neopestalotiopsis sp. and pale luteous to orange for N. rosae. Spores for both groups were five-celled with three median versicolored cells. Mycelial growth and spore production were higher for the new Neopestalotiopsis sp. isolates. Isolates from both groups were pathogenic to strawberry roots and crowns. However, the new Neopestalotiopsis sp. proved more aggressive in fruit and leaf inoculation tests, confirming observations from the recent outbreaks in commercial strawberry fields in Florida.

scholarly journals Effects of Long-Wave UV Light on Monilinia Growth and Identification of Species

Plant Disease ◽  
1999 ◽  
Vol 83 (1) ◽  
pp. 62-65 ◽  
Author(s):  
A. De Cal ◽  
P. Melgarejo
Keyword(s):  
Short Distance ◽  
Mycelial Growth ◽  
Uv Light ◽  
Dark Period ◽  
Morphological Characteristics ◽  
Potato Dextrose Agar ◽  
Long Wave ◽  
The World ◽  
Identification Of Species ◽  
Respective Species

The effect of long-wave UV/dark period on mycelial growth of 46 isolates of Monilinia sp. collected in Spain and 16 isolates collected from other parts of the world was investigated. Typical isolates of M. laxa, M. fructigena, and M. fructicola were grown in the dark and identified by morphological characteristics. Long-wave UV/dark conditions reduced the growth rates of M. laxa, M. fructigena, and M. fructicola on potato dextrose agar. All isolates of M. fructigena grew more slowly than those of M. fructicola. Typical and atypical isolates of M. fructigena and M. fructicola were placed in their respective species based on long-wave UV/dark growth rate data. M. laxa isolates were readily distinguished by the short distance from their conidium to the first germ tube branch. The involvement of different photoreceptors in photoresponses by M. fructicola and M. fructigena is discussed. Differences in mycelial growth under long-wave UV may be a useful tool to identify Monilinia spp.

scholarly journals Research Regarding the Morphological Characteristics of Calendula Officinalis Local

2012 ◽  
Vol 69 (1) ◽  
Author(s):  
Cristina ZEPA ZEPA CORADINI ◽  
Valeriu TABÄ‚RÄ‚ ◽  
Doru PETANEC ◽  
Lavinia MICU ◽  
Irina PETRESCU ◽  
...  
Keyword(s):  
Local Population ◽  
Economic Value ◽  
Morphological Characteristics ◽  
Morphological Features ◽  
Morphological Aspect ◽  
Calendula Officinalis ◽  
Experimental Field ◽  
Wide Range ◽  
Polymorphic Species ◽  
Flowering Process

Marigolds have an important economic value which let to an increase production and cultivation being thus used in a wide range of fields. One of the basic elements regarding marigolds production is represented by anthodia with flowers and seeds determined by the blossom and the number of anthodium on the plant. The plant’s blossom is determined by the n umber of lingulate flowers from the external side of the anthodium where seeds grow. Calendula is a polymorphic species, forming during its evolution not only flowering, but also semi-flowering anthodia and simple flowers. In the experimental field of UASVM Timisoara we performed a series of research regarding the morphological features of six local population of marigold from the western part of the country. Flowers’ blooming proved to have a different evolution due to the influence manifested by the local population and also by the harvest results. This blooming phenomenon proved to highly influence the production elements. Blooming influence upon anthodia mass of seeds proved to be as similar as in case of anthodia with flowers. At the beginning of the harvest period, the anthodia mass of seeds proved to be maxim. According to the information collected during research, anthodia flowering fails to reestablish during harvest. The flowering process influences the number and the size of the seeds. Moreover, flowers blooming process may lead to the formation of homogenous seeds, not only in size, but also as regards their morphological aspect. The seeds from the flowering anthodia proved to be homogenous in comparison with other anthodia and had even better technological qualities.

scholarly journals PENGARUH NAUNGAN TERHADAP PERTUMBUHAN SEMAI MAKILA (Litsea angulata)

2017 ◽  
Vol 1 (3) ◽  
pp. 262
Author(s):  
Edom Bayau
Keyword(s):  
Experimental Design ◽  
Rheumatic Diseases ◽  
Seedling Growth ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Good For

   Makila (Litsea angulata) is a type of commercial timber and is very attractive to many people because of good quality and can also be used as a medicine in curing rheumatic diseases should be assigned the paramount concern. cultivation / reproduce makila can be done in a fast way is through the nursery in the field. in nurseries should also pay attention to things that affect makila, one of which is related to the intensity of sunlight makila seedling growth. The research conducted at the location of the greenhouse Faculty of Agriculture, University of Pattimura which take place september until november 2014 with the aim to determine the seedling growth makila (Litsea angulata) to determine what percentage of shade is good for seedling growth makila (Litsea angulata). The experimental design used was completely randomized design (CRD) with 5 (five) treatments, the seedlings without using Shade, Shade 30 percent, 50 percent Shade, Shade 65 percent, and Shade 75 percent. The results showed that the seedling Makila (Litsea angulata) is a kind of tolerance with all shade or kind makila able to grow in places that are open and shaded place.

scholarly journals CNSL Concentration and Natural Dyes Effects in Formulation of Botanical Pesticide on Sitophilus zeamais and Maize Seed Quality

2020 ◽  
Vol 4 (1) ◽  
pp. 1-9
Author(s):  
Dian Astriani ◽  
Wafit Dinarto ◽  
Aji Jatmiko
Keyword(s):  
Seed Quality ◽  
Sitophilus Zeamais ◽  
Natural Dyes ◽  
Maize Weevil ◽  
Maize Seeds ◽  
Botanical Pesticide ◽  
Randomized Design ◽  
Efficiency And Effectiveness ◽  
Push Down

Maize is an important commodity in Indonesia and is vulnerable to maize weevil (Sitophilus zeamais) during storage which can cause a 100% yield loss. Chasew Nut Shell Liquid (CNSL) is a botanical pesticide able to control S. zeamais. Increasing CNSL efficiency and effectiveness requires additional adjuvants, such as solvents and dyes. Natural dyes addition is intended to provide seed markers. This study aimed to determine the best CNSL and natural dye formulation to control maize weevil and maintain maize seeds quality during storage. The research was conducted from August to October 2018 at the Agronomy Laboratory, Faculty of Agroindustry, Yogyakarta Mercu Buana University. The research was a single factor consisted of seven treatments with four replications arranged in a completely randomized design (CRD). Treatments were combinations of CNSL concentrations with the addition of natural dyes from suji leaf and teak leaves with one untreated control. The results of the research showed that CNSL as a seed treatment botanical pesticide could cause mortality and suppress S. zeamais populations and maintain the quality of maize seeds during storage for 12 weeks. Treatments with 20% CNSL formulation concentration and the addition of natural dyes both leaf pleomele or teak leaves could cause higher mortality and push down more populations and also could better maintain seed quality than 10 and 0% CNSL formulation concentrations.

scholarly journals First Report of Colletotrichum gloeosporioides on Chinese Rose in Argentina

Plant Disease ◽  
2000 ◽  
Vol 84 (12) ◽  
pp. 1345-1345 ◽  
Author(s):  
M. C. Rivera ◽  
E. R. Wright ◽  
S. Carballo
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Buenos Aires ◽  
Morphological Characteristics ◽  
Disease Prevalence ◽  
Potato Dextrose Agar ◽  
Conidial Suspension ◽  
First Report ◽  
Hibiscus Rosa Sinensis ◽  
Leaf Drop ◽  
Stems And Leaves

Chinese rose (Hibiscus rosa-sinensis L.) is a shrub frequently planted in Argentina. In November 1999, dieback and anthracnose symptoms were detected on stems and leaves of plants cv. Hawaii cultivated in Buenos Aires. Disease prevalence was 50%. Pieces of infected tissues were surface-sterilized for 1 min in 2% NaOCl, plated on potato-dextrose agar and incubated at 24 ± 2°C. The isolate that was consistently recovered from diseased tissues was identified as Colletotrichum gloeosporioides (Penz.) Penz. and Sacc., based on morphological characteristics (1,2). Teleomorph stage was not observed. Inoculation for pathogenicity testing was carried out by spraying a conidial suspension (6.5 × 106 conidia per ml) on plants with previously punctured leaves and pruned stems. Inoculated plants with unwounded tissues, as well as noninoculated controls, were included. Five replications of each treatment were done. Plants were incubated in moist chambers at 24°C. Whitish areas of 0.3 to 0.5 cm diameter surrounded by a purple halo developed on all punctured leaves within 10 days. Stem blight and leaf drop were observed. The center of the lesions was covered by black acervuli 14 days after inoculation. Unwounded and noninoculated controls remained symptomless. The pathogen was reisolated from inoculated leaves, completing Koch's postulates. This is the first report of C. gloeosporioides causing disease on Chinese rose in Argentina. References: (1) J. A. Bailey and M. J. Jeger, eds. 1992. Colletotrichum. CAB International, Surrey, England. (2) B. C. Sutton. 1980. The Coelomycetes. CMI, Kew.

scholarly journals Efficacy of Liquid Smoke Produced from Medang Wood (Cinnamomum sp.) against Schizophyllum commune

2021 ◽  
Vol 9 (2) ◽  
pp. 269
Author(s):  
Rizka Diah Permana ◽  
Hasan Ashari Oramahi ◽  
Farah Diba
Keyword(s):  
Growth Inhibition ◽  
Pyrolysis Temperature ◽  
Schizophyllum Commune ◽  
Fungal Growth ◽  
Potato Dextrose Agar ◽  
Liquid Smoke ◽  
Antifungal Properties ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Fungus Growth

The efficacy of liquid smoke obtained from medang wood (Cinnamomum sp.)against Schizophyllum commune fungus was evaluated. This study aims to evaluate the antifungal properties of liquid smoke from medang against S. commune fungus. Potato Dextrose Agar (PDA) medium was used to determine the efficacy of the liquid smoke of medang wood on S. commune fungus growth. Three kinds of liquid smoke were obtained from the pyrolysis of medang wood at 370, 400, and 430°C. The efficacy of liquid smoke from medang wood for antifungal is a factorial 3 by 4 in a completely randomized design; the first factor was pyrolysis temperature of liquid smoke from medang (370, 400, and 430°C), and the second factor was the treatment of concentration of liquid smoke from medang wood (0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0%, v/v). The results showed that pyrolysis temperature affected liquid smoke from medang, and the concentration of liquid smoke was significantly different for inhibition of fungal growth. The results indicated that the pyrolysis temperature of liquid smoke production and the concentration of liquid smoke had a significant effect on S. commune fungus growth inhibition. Medang wood liquid smoke effectively inhibited the growth of S. commune fungus about 98.57% at a concentration of 2.5% with liquid smoke pyrolysis temperature used is 430°C.Keywords: efficacy, inhibition of fungal growth, liquid smoke, medang wood, Schizophyllum commune

scholarly journals Pemanfaatan sukun muda untuk peningkatan nilai gizi abon ikan tongkol

2021 ◽  
Vol 2 (2) ◽  
pp. 185
Author(s):  
Eva Fitriyaningsih
Keyword(s):  
Experimental Design ◽  
Nutritional Value ◽  
Economic Value ◽  
Chemical Properties ◽  
Animal Nutrition ◽  
P Value ◽  
Randomized Design ◽  
Study Results ◽  
Completely Randomized Design ◽  
Actual Participation

Background: Using young breadfruit to increase the breadfruit usability and economic value, one of the efforts is through the enrichment of the type of processed breadfruit products, breadfruit provides nutritional value that is beneficial to health, one of the help of breadfruit is increasing breadfruit for shredded products.Objectives: This study aims to find out how to improve young breadfruit 20%, 30%, 40% of the chemical properties (carbohydrate, protein, and fiber) of shredded tuna.Methods: This study used an experimental design with a non-factorial Completely Randomized Design (CRD) with three preparations and three repetitions. Then the chemical tests (carbohydrate, protein, and fiber) at the Laboratory of Animal Nutrition and Feed Technology of the Faculty of Agriculture, Syiah Kuala University, Banda Aceh.Results: The study results showed actual participation in young breadfruit 20%, 30%, and 40% of the chemical properties (carbohydrate, protein, and fiber) in shredded tuna with a p-value < 0.05.Conclusion: Agreeing that 20%, 30%, and 40% of young breadfruit were approved for shredded tuna's chemical properties (carbohydrate, protein, and fiber).

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