scholarly journals UJI EFEKTIVITAS SALEP EKSTRAK ETANOL RIMPANG JERINGAU (Acorus calamus L.) TERHADAP PENYEMBUHAN LUKA SAYAT

2020 ◽  
Vol 3 (1) ◽  
pp. 13-20
Author(s):  
Linta Meliala
Keyword(s):  
Test Group ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Negative Control ◽  
Effective Control ◽  
Acorus Calamus ◽  
Positive Group ◽  
Ointment Base

Introduction: Jeringau rhizome plants are one of the traditional plants which are used as antibacterial. Method: The extraction process was carried out by maceration using 80% ethanol. The effectiveness of jeringau rhizome testing was divided into 5 treatments, the positive group used betadine ointment, negative control using an ointment base, and the test group used ethanol extract ointment with 5%, 10% and 15% concentration. Cut wounds given to the rabbit's back with a 2 cm long wound and 2 mm deep, the ointment applied twice a day for 13 days and measured using a ruler or calipers. Result: The results show that Jeringau rhizome contains flavonoid, tannin, saponin, and alkaloid metabolites which can be used as wound heal wound healers. This study aims to determine its effectiveness as a wound healer wound. Conclusions: indicate that the ointment extract jeringau rhizome at a concentration of 15% is the most effective control as a wound healer but the ointment extract of jeringau rhizome ethanol extract is less effective when compared to betadine ointment as a wound healer in rabbits. .

scholarly journals Efek Antidiare Ekstrak Etanol Daun Kelor (Moringa oleifera L.) Pada Mencit Putih Jantan

2020 ◽  
Vol 6 (1) ◽  
pp. 35-39
Author(s):  
Rizal Fauzi ◽  
◽  
Annisa Fatmawati ◽  
Emelda Emelda ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Intestinal Motility ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Extraction Process ◽  
Negative Control ◽  
Control Group ◽  
Average Value ◽  
Normal Individuals

Diarrhea is an increase frequency and decrease consistency of fecal when compared with the bowel condition of normal individuals. The high incidence of acute and chronic diarrhea encourages researchers to keep trying to find new antidiarrheal drugs, especially from plants. The leaves of Moringa (Moringa oleifera Lam) contain tannins which are polyphenolic compounds and has benefits as anantidiarrheal.This study aims to determine the activity of the ethanol extract of moringa leaves as an antidiarrheal by looking at intestinal motility. The extraction process was carried out by maceration method using ethanol 70%..The result research is the average value of the treatment ratio is smaller than the negative control group. The average value of the ratio of the group was given by ethanol extract of moringa leaves at a dose of 9.1 mg / 20 g body weight was 0.664; ethanol extract of moringa leaves at dose of 18.2 mg / 20 g body weight was 0.434 and ethanol extract of moringa leaves at dose of 36.4 mg / 20 g body weight was 0.389. From this value, it is known that the ethanol extract of Moringa leaves has an antidiarrheal effect by inhibiting intestinal motility. The effect of the ethanol extract of Moringa leaves in inhibiting intestinal motility is best at a dose of 36.4 mg / 20 g body weight with an average value ratio of 0.389.

scholarly journals EFEK PENYEMBUHAN LUKA BAKAR GEL KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Vernonia Amygdalina) DAN EKSTRAKETANOLDAUNBANGUN–BANGUN(ColeusamboinicusLour)

2020 ◽  
Vol 3 (1) ◽  
pp. 57-61
Author(s):  
Nina Irmayanti Harahap
Keyword(s):  
Ethanol Extract ◽  
Effective Control ◽  
Vernonia Amygdalina ◽  
Positive Group ◽  
Healing Wound ◽  
Coleus Amboinicus

african leaves ( Vernonia Amygdalina )and wake-up leaves (Coleus amboinicus Lour)are plants traditional plants that are used as medicine. Leaves african which is contain flavonoid, saponin, tanin and leaves wake up which is contain flavonoid, tanin, saponin and steroid. This study to find aims out as effect healing of burns.Method : Sample in this study was african leaves and wake-up leaves which were taken purposively without comparing sampel from other regions, then extrated by maceration using ethanol 96 %. Result : Testing of rhe healing effects of burns can be divided in five treatment, positive group using biolacenton , negatif group using basis gel and the last group using the extract ethanol gel EEDA and EEDB concentrations of 4%+2,5%,8%+5%,16%+10%. Given burns to the back of rabbits length with of 2cm, and the gel is applied twice a day for 11 day and diameter measured of the wound. Conclusion : The combination of EEDA and EEDA the ethanol extract the concentration of 16% + 10% was the effective control for healing burns. When compared bioplacenton gel as a healing wound in rabbit.

scholarly journals Effect of Gooseberry (Physalis angulata) Ethanol Extract in Wistar Rats Carrageenan-Induced Paw Oedema

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Ami Nurlatifah ◽  
Eka Hendryanny ◽  
Yuniarti Yuniarti
Keyword(s):  
Test Group ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sample Test ◽  
Anti Inflammatory ◽  
One Way Anova ◽  
Edema Inhibition

Gooseberry is an herbaceous plant that contains flavonoids. Flavonoid is one of the secondary metabolites that have an anti-inflammatory effect. This study aims to determine the effect of using ethanol extract of gooseberry as an anti-inflammatory in carrageenan-induced paw edema. This study was in vivo experimental laboratory using a completely randomized design of 25 Wistar rats and divided into five groups. The negative control group was given carboxymethylcellulose. The positive control group has given diclofenac sodium 27 mg/200 gBW. The sample test group has given ethanol extract of gooseberry with 3.6 mg/200 gBW, 5.4 mg/200 gBW, and 7.2 mg/200 gBW dosage. Paw rat’s inflammation induced by injecting carrageenan and measured from 1st to 6th hour using a pletismometer. This study has conducted at Pharmacology Laboratory, Universitas Islam Bandung, and the Laboratory of Therapy and Pharmacology, Universitas Padjajaran, from June to September 2019. The result of average edema volume paw rats using the Kruskal-Wallis test on the 6th hour was p=0.02 (p<0.05). The Mann-Whitney test was p<0.05, showing differences between negative control and positive control and sample test groups. One-way ANOVA test on the percentage of edema inhibition between positive control and sample test group had p=0.107. It shows no significant difference. An effect of ethanol of extract of gooseberries as an anti-inflammatory with the highest percentage of edema inhibition is 5.4 mg/200 gBW dosage. The flavonoid content in gooseberries is thought to inhibit the formation of prostaglandins by inhibiting the cyclooxygenase enzyme. In conclusion, the ethanol extract of gooseberry can be anti-inflammatory. EFEK EKSTRAK ETANOL CIPLUKAN (PHYSALIS ANGULATA) TERHADAP EDEMA TELAPAK KAKI TIKUS GALUR WISTAR YANG DIINDUKSI KARAGENANCiplukan adalah tanaman herbal yang mengandung flavonoid. Flavonoid merupakan salah satu metabolit sekunder yang dapat memberikan efek antiinflamasi. Penelitian ini bertujuan mengetahui pengaruh penggunaan ekstrak etanol ciplukan sebagai antiinflamasi pada tikus yang diinduksi karagenan. Penelitian ini merupakan penelitian laboratorium eksperimental in vivo menggunakan desain rancangan acak lengkap pada 25 ekor tikus galur Wistar yang terbagi ke dalam lima kelompok. Kelompok kontrol negatif diberi carboxymethilcellulose. Kontrol positif diberi sodium diklofenak 27 mg/200 gBB. Kelompok uji diberi ekstrak etanol ciplukan dengan dosis 3,6 mg/200 gBB; 5,4 mg/200 gBB; dan 7,2 mg/200 gBB. Induksi inflamasi dilakukan dengan menginjeksikan karagenan pada telapak kaki tikus, lalu diukur menggunakan pletismometer dari jam ke-1 hingga jam ke-6. Penelitian ini dilakukan di Laboratorium Farmasi, Universitas Islam Bandung dan Laboratorium Farmasi dan Terapi, Universitas Padjajaran dari bulan Juni hingga September 2019. Volume rerata telapak kaki tikus pada jam ke-6 menggunakan Uji Kruskal-Wallis adalah p=0,02 (p<0,05). Hasil Uji Mann-Whitney diperoleh p<0,05 yang menunjukkan terdapat perbedaan bermakna antara kontrol negatif dan kontrol positif serta kelompok uji. Uji one-way ANOVA pada persentase penghambatan edema antara kontrol positif dan kelompok uji diperoleh p=0,107 yang menunjukkan tidak terdapat perbedaan yang bermakna. Terdapat pengaruh ekstrak etanol ciplukan sebagai antiinflamasi dengan persentase penghambatan edema tertinggi pada dosis 5,4 mg/200 gBB. Kandungan flavonoid pada ciplukan diduga mampu menghambat pembentukan prostaglandin dengan menginhibisi enzim siklooksigenase. Simpulan penelitian ini adalah ekstrak etanol ciplukan dapat digunakan sebagai antiinflamasi.

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN BAWANG DAYAK (Eleutherine Palmifolia L. Merr) TERHADAP KADAR GULA DARAH MENCIT PUTIH (Mus muscullus) YANG DIINDUKSI GLUKOSA

2020 ◽  
Vol 5 (2) ◽  
pp. 288-297
Author(s):  
Eka Kumalasari ◽  
◽  
Syifa Maharani ◽  
Aditya Maulana Perdana Putra ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Test Group ◽  
Ethanol Extract ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Sugar Levels

Diabetes Mellitus (DM) is a collection of metabolic symptoms that arise in a person caused by an increase in blood glucose due to damage to insulin secretion or resistance to insulin or both. Diabetes mellitus medication can be either parenteral or oral which can cause side effects in long-term use such as mild diarrhea, bloating, fatigue acidosis, even hypoglycemic renal impairment. To prevent it can use alternative medicine using Dayak onion (Eleutherine palmifolia (L) Merr.). One of the active substances contained in the Dayak onion is the flavonoids. Flavonoids have antidiabetic activity through their functions as antioxidants. The study aims to determine the activity of Dayak scallion Extract (Eleutherine palmifolia (L) Merr.) To decrease blood sugar levels (Mus muscullus), which is glucose-induced. The research was conducted with research draft pre and post test with control group design. The mice used amounted to 25 male white mice that were divided into 5 groups i.e. negative control (CMC Na 0.5%), control positive (Glibenklamid 3 mg/kgBB), Dayak onion-leaf ethanol extract at a dose of 50 mg/kgBB, 100 mg/kgBB and 200 mg/kgBB. Observations of the decrease in blood sugar levels of each test group were conducted on the same day, 30 minutes, 60 and 90 after hyperglycemia. Results showed that Dayak onion leaves ethanol extract had activity as a lowering of blood sugar levels in glucose-induced white mice

scholarly journals Measuring the Antioxidant Effect of Limnocharis Flava on Malondialdehyde Activities in Livers of Alloxan-induced Diabetic Rats

2021 ◽  
Vol 19 (2) ◽  
pp. 181-186
Author(s):  
Yithro Serang ◽  
Virda Laili
Keyword(s):  
Test Group ◽  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antioxidant Effect ◽  
White Rats ◽  
The One ◽  
One Way Anova ◽  
Group 1

Antioxidants are substances counteracting the effects of free radicals. Antioxidants are formed in several forms, encompassing vitamins, minerals, and phytochemicals. Genjer or yellow velvet leaves (Limnocharis Flava) is an aquatic plant potentially beneficial alternative to natural antioxidants. This study aims to measure the antioxidant effect from the yellow velvetleaf ethanol extract on malondialdehyde (MDA) activities in livers of diabetic rats (Rattus novergicus) induced by alloxan in three different doses. The tested animals were randomly divided into five groups comprising six white rats previously induced with alloxan by intra-peritoneal injection. Group 1 was the negative control provided 0.5% CMC Na while group 2 was a positive control administered 0.45 mg glibenclamide. On the other hand, groups 3, 4, and 5 were the experimental groups that provided the extract of yellow velvetleaf as many as 32.5 mg/kg body weight (BW) ethanol, 65 mg/kg BW ethanol extract, and 130 mg/kg BW, respectively. Furthermore, the extracts were administered once on the 7th, 14th, and 21st days; the parameters observed were MDA activities in the rats’ livers. The Thiobarbituric Acid Reactive Substances (TBARs) method was then employed to investigate these actions. The one-way ANOVA was administered to examine the acquired data from the examination, followed by a post hoc test. The average points of the MDA level from group 1 to group 5 were 10.78 0.33, 3.71 + 0.19, 7.25 + 0.26, 5.76 + 0.25, and 4.01 + 0.22, correspondingly, according to the results. The one-way ANOVA test revealed that MDA levels in each test group were significantly different (p 0.05). Based on these findings, it may be concluded that feeding ethanol extract from yellow velvet leaves to diabetic rats reduces MDA levels in their livers, therefore preserving cells from harm.

scholarly journals Potensi Ekstrak Etanol dan Fraksi-Fraksinya Dari Tiga Varietas Jahe Sebagai Agen Antibakteri Terhadap Staphylococcus aureus

2020 ◽  
Vol 17 (1) ◽  
pp. 9-16
Author(s):  
Dewi Dianasari ◽  
Endah Puspitasari ◽  
Indah Yulia Ningsih ◽  
Bawon Triatmoko ◽  
Fauzia Ken Nasititi
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Test Group ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Inhibition Zone ◽  
Negative Control ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Red Ginger

One of the natural ingredients that to be used to inhibit bacterial growth is the Zingiber officinale. In Indonesia, there are 3 types of ginger which are distinguished by morphology, namely ‘emprit ginger (Zingiber officinale var. Amarum), ‘ gajah’ ginger (Zingiber officinale var. Officinarum), and red ginger (Zingiber officinale var. Rubrum). This research was conducted to determine the activity of extracts and fractions of three ginger varieties in inhibiting the growth of Staphylococcus aureus using the disc diffusion method. The test group consisted of ethanol extract, n-hexane fraction, and ethyl acetate fraction from 3 ginger varieties, concentrations of 5, 10, 20% w / v, gentamicin 10 μg (positive control) and 10% DMSO (negative control). The test results showed that ethanol extracts and fractions from the rhizomes of three varieties of ginger have the ability to inhibit S. aureus at concentrations of 5, 10, and 20% w / v, increasing the concentration will increase the diameter of the inhibition zone. At a concentration of 20% of all samples, the one with the greatest resistance was the n-hexane fraction (diameter of inhibitory zone of emprit ginger 9.80 mm, elephant ginger 9.78 mm, and red ginger 9.90 mm). The difference in ginger variety only affects the antibacterial activity of the ethanol extract, but not the n-hexane and ethyl acetate fractions.

scholarly journals Effect of Drumstick (Moringa Oleifera Lam) Leaves Ethanol Extract on Anopheles Aconitus L. Third Instar Larvae Mortality

2020 ◽  
Author(s):  
Yuzana Maung ◽  
◽  
Sutartinah Sri Handayani ◽  
Lukman Aryoseto ◽  
◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Test Group ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Central Java ◽  
Negative Effect ◽  
Moringa Oleifera Lam ◽  
Third Instar Larvae

Background: Synthetic insecticides may have the negative effect to nature. Many studies suggested the applications of botanical larvicides as an alternative replacement for synthetic insecticides. This study aimed to examine the effect of Moringa oleifera Lam leaves ethanol extract on the Anopheles aconitus L. third instar larvae mortality. Subjects and Method: This was a laboratory experimental with post-test only con-trol group design conducted at Salatiga, Central Java, in November 2016. A total of 150 Anopheles aconitus L. third instar larvae was selected by convenience sampling method and divided into 6 groups in which contained replication of 25 larvae. One negative control group was added 100 ml distilled water. The other treatment groups were 1 mg/ 100 ml, 10 mg/ 100 ml, 20 mg/ 100 ml, 30 mg/ 100 ml, and 40 mg/ 100 ml of Moringa oleifera Lam leaves ethanol extract. Each test group was repeated four times. The dependent variable was mortality of the larvae. The independent variable was different concentrations of Moringa oleifera Lam leaves ethanol extract. The observation of larvae mortality was conducted after 48 hours of exposure with extract. The data were analyzed by Kruskal-Wallis. Results: Mean of the mortality of Anopheles aconitus L. third instar larvae was dif-ferent in each group, and it was statistically significant (p= 0.008). Conclusion: Moringa oleifera Lam leaves ethanol extract has effect on mortality of Anopheles aconitus L. third instar larvae. Keywords: Moringa oleifera Lam, Anopheles aconitus L., ethanol extract, mortality Correspondence: Yuzana Maung. Masters Program in Public Health, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta, 57126, Central Java, Indonesia. Email: [email protected]. Mobile: +6281295346614. DOI: https://doi.org/10.26911/the7thicph.05.59

UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

2020 ◽  
pp. 68-73
Author(s):  
Yuni Asri Mulatsih Agami ◽  
Eka Wisnu Kusuma
Keyword(s):  
Oxidative Stress ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Experimental Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Negative Group ◽  
Ic50 Value ◽  
Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7%  Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7  setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif.    Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii  in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Antihyperglycemic activity of Centella asiatica (L.) Urb. leaf ethanol extract SNEDDS in zebrafish (Danio rerio)

2021 ◽  
Vol 19 (1) ◽  
pp. 184-188
Author(s):  
Farida Hayati ◽  
Lutfi Chabib ◽  
Faiza Dea Sekarraras ◽  
Wan Syarifah Faizah
Keyword(s):  
Glucose Solution ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Peg 400 ◽  
Good Preparation

Abstract This study aimed to identify the effectiveness of SNEDDS of Pegagan Leaf Ethanol Extract (PLE) to reduce fasting blood glucose (FBG) levels in zebrafish. Centella asiatica (L.) Urb. or pegagan is among the medicinal plants widely used to treat diabetes in Indonesia. Maceration was employed with 70% ethanol to obtain a viscous extract for the formulation of SNEDDS with Capryol 90, Tween 80, and PEG 400 (1:6:3). Antihyperglycemic testing was conducted on five groups, consisting of normal, positive control, negative control, P I treatment, and P II treatment. On Day 1, all except the normal group was induced with 300 mg alloxan and soaked in 2% glucose solution for 7 days. On day 8, the treatment consisted of 25 mg/2 L metformin for the positive control, 100 mg/2 L SNEDDS for P I, 200 mg/2 L SNEDDS for P II, and no treatment for the negative control. The SNEDDS characterization obtained 100.6 ± 3.12 nm particle size and −7.93 ± 0.66 mV zeta potential, indicating that the SNEDDS had fulfilled the requirements of good preparation. The antidiabetic activity test found a 69.90% decline in FBG levels in 100 mg/2 L SNEDDS and 72.20% in 200 mg/2 L SNEDDS.

Sign in / Sign up

Export Citation Format

Share Document