scholarly journals Avaliação das condições higiênico-sanitárias e treinamento de manipuladores de alimentos em instituições públicas no município de Ipaumirim-CE

2021 ◽  
Vol 12 (3) ◽  
pp. 339-348
Author(s):  
Naiara Menezes Bezerra ◽  
Kássia Raffaela Roque Silva ◽  
Edsania Princelânia Xavier Nésio ◽  
Lucélia Kátia De Lima ◽  
Juliana Maria Guedes de Oliveira
Keyword(s):  
Staphylococcus Aureus ◽  
High Rate ◽  
Microbiological Analysis ◽  
Food Handlers ◽  
Food Services ◽  
National Agency ◽  
Before And After ◽  
Sanitary Conditions ◽  
The City ◽  
And Training

As condições higiênico-sanitárias dos alimentos são de grande importância para a saúde pública, principalmente quando são oferecidos à coletividade, como em escolas. Dessa forma, o conhecimento acerca das Boas Práticas de Fabricação (BPF) na produção da alimentação escolar, além de essencial, é mandatório, pois é uma exigência legal. Este trabalho incluiu consultorias e atividades de assessoria, através de avaliações e capacitações nos serviços de alimentação em escolas da cidade de Ipaumirim – CE. Toda a metodologia utilizada foi baseada nas resoluções Resolução de Diretoria Colegiada (RDC) nº 216 de 2004 e RDC nº 275 de 2002 da Agência Nacional de Vigilância Sanitária (ANVISA). Primeiramente, foi realizada a aplicação de um checklist de condições higiênico-sanitárias nas escolas municipal (A) e estadual (B), seguida de avaliação das condições higiênicas dos manipuladores, por meio de análises microbiológicas. Por último, foi realizada uma capacitação desses manipuladores. A aplicação do checklist apontou um alto índice de inadequações. Nas análises microbiológicas feitas nos utensílios, a tábua de corte da escola A apresentou contaminação de Staphylococcus aureus. Nos demais utensílios e na escola B, não houve crescimento para os microrganismos analisados. Os resultados indicam condições estruturais precárias das escolas, evidenciando a necessidade de adequação às BPF. Contudo, os manipuladores apresentaram resistência, tendo em vista a comparação das adequações antes e após a capacitação realizada, em especial na escola A, ficando clara a necessidade de serem realizadas mais ações e orientações sobre o cuidado higiênico-sanitário dos alimentos. Palavras-chave: Merenda escolar; Higiene dos alimentos; Boas práticas; Capacitação Evaluation of hygienic-sanitary conditions and training of food handlers in public institutions in the municipality of Ipaumirim-CE Abstract: Hygienic and sanitary conditions of schools' food services have great importance for public health, especially when offered to the community, as in schools. Thus, the knowledge about Good Manufacturing Practices (GMP) in producing school meals, besides being essential, is mandatory as a legal requirement. This work included consulting and advisory activities through evaluations and training in food services in schools in the city of Ipaumirim, Ceará, Brazil. The methodology was based on resolutions RDC No. 216 of 2004 and RDC 2002 of ANVISA (Brazilian National Agency of Sanitary Vigilance). The first step was to apply a hygienic and sanitary conditions checklist in 2 schools (School A: district school and School B: state school). The second step was to evaluate the hygienic conditions of food handlers through microbiological analysis. The last step was the food handlers training. The checklist showed a high rate of inadequacies. The microbiological evaluation showed contamination by Staphylococcus aureus on the cutting board from school A. At school B, there was no growth for the analyzed microorganisms. The checklist results are compatible with the precarious structural conditions of schools, highlighting the need to adapt to GMP. However, the handlers showed resistance, given the comparison of the adaptations before and after the training, especially at school A, making it clear the need for further actions and guidance on hygienic-sanitary food care. Keywords: School meal, Food hygiene, Good practices, Capacitation

scholarly journals Análise Microbiológica de Batons de Uso Compartilhado na Cidade de Juazeiro do Norte-CE

2021 ◽  
Vol 24 (5-esp.) ◽  
pp. 636-639
Author(s):  
Luana Romão Rodrigues ◽  
João Victor de Freitas Menezes ◽  
Dárcio Luiz de Sousa Júnior ◽  
Rafael de Carvalho Mendes
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Scientific Work ◽  
Quality Standard ◽  
Microbiological Analysis ◽  
Great Circulation ◽  
Effective Use ◽  
The City

A cosmetologia é a ciência que engloba e estuda os cosméticos, desde conceitos iniciais até a sua fabricação, tornando-se uma ciência multidisciplinar. Para garantia de um uso seguro e eficaz dos cosméticos é necessário a realização de análises para se conseguir um padrão de qualidade. Quando há indícios de contaminação por microrganismos no cosmético, diversos problemas podem ser encontrados, como a ocorrência de patologias específicas, causando altos riscos e complicações à saúde do usuário. O objetivo deste trabalho foi investigar qualitativamente através de análises microbiológicas a qualidade dos produtos cosméticos de maquiagem usados de forma compartilhada, utilizando amostras colhidas aleatoriamente em uma loja de varejo, que foi selecionada por ser de grande circulação, da cidade de Juazeiro do Norte, Ceará. Nesta pesquisa foram analisadas oito amostras coletadas durante o período de outubro e novembro de 2017. As análises que foram realizadas seguiram a Farmacopeia brasileira. Como resultado obteve-se um percentual de 87,5 % de contaminação microbiana, evidenciando a presença de Staphylococcus aureus, Escherichia coli, Enterococcus sp. e Pseudomonas aeruginosa, sendo que apenas uma das oito amostras analisadas mostrou-se adequada para uso seguindo os limites de aceitação microbiológicas permitidos por lei. Baseados nesses resultados foi possível concluir que o índice de contaminação das amostras de batons que são utilizadas de modo compartilhado são potencialmente maiores. Com isso, intensifica-se a necessidade de medidas que impeçam o compartilhamento dos produtos cosméticos, com a finalidade de diminuir o risco de uma infecção coletiva.   Palavras-chave: Cosméticos. Uso compartilhado. Contaminação.   Abstract Cosmetology is the science that encompasses and studies cosmetics, from initial concepts to their manufacture, becoming a multidisciplinary science. To guarantee a safe and effective use of cosmetics it is necessary to carry out analyzes to achieve a quality standard. When there is evidence of contamination by microorganisms in the cosmetic, several problems can be found, such as the occurrence of specific pathologies, causing high risks and complications to the health of the user. The objective of this scientific work was to qualitatively investigate through microbiological analysis the quality of cosmetic makeup products used in a shared way, using samples collected randomly in a retail store, which was selected because it is of great circulation, in the city of Juazeiro do Norte, Ceará. In this research, eight (8) samples collected during the period of October and November 2017 were analyzed. The analyzes that were carried out followed the Brazilian Pharmacopoeia. As a result, a percentage of 87.5 % of microbial contamination was obtained, showing the presence of Staphylococcus aureus, Escherichia coli, Enterococcus sp. and Pseudomonas aeruginosa, with only one of the eight samples analyzed being suitable for use following the limits of microbiological acceptance allowed by law. Based on these results it was possible to conclude that the contamination index of the lipstick samples that are used in a shared way are potentially higher. As a result, the need for measures to prevent the sharing of cosmetic products is intensified, in order to reduce the risk of a collective infection.   Keywords: Cosmetics. Shared Use. Contamination.

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

Mechanical ventilator as source of cross-infection: implications for the nursing team

2011 ◽  
Vol 5 (9) ◽  
pp. 2206
Author(s):  
Marquiony Marques dos Santos ◽  
Izabel Calixa de Alcântara ◽  
Diego Bonfada ◽  
Eudes Euler de Souza Lucena ◽  
Cristyanne Samara Miranda de Holanda
Keyword(s):  
Staphylococcus Aureus ◽  
Intensive Care ◽  
Pathogenic Bacteria ◽  
Cross Infection ◽  
Health Staff ◽  
Microbiological Analysis ◽  
Mechanical Ventilator ◽  
Nursing Team ◽  
Before And After ◽  
Terapia Intensiva

ABSTRACTObjective: to identify the presence of pathogenic bacteria in the annexes of the mechanical ventilator (MV) before and after its assembly, and discuss the decontamination accomplished by the nursing team of a regional hospital. Method: this is a cross-sectional study, with an exploratory descriptive design and a quantitative nature. The data collection was carried out with swabs in five specific points of the internal parts of the annexes of mechanical ventilation, before and after its assembly, in an intensive care unit. The material collected was seeded in culture media for microorganisms of medical importance and identified at the level of genus and species through biochemical tests. The results were analyzed through the software SPSS 17.0. Results: before the assembly of the MV annexes, there was presence of 250 UFC/mm³ of Staphylococcus aureus; after the assembly of the MV annexes, 500 UFC/mm³ of Staphylococcus aureus, 250 UFC/mm³ of Enterococcus sp., and 1,000 UFC/mm³ of Enterobacter sp. were calculated. Conclusion: there were errors in the disinfection process, as well as in the manipulation of the MV annexes, presenting contamination in both moments under study. Measures of continued education for health staff, particularly for nursing team, can reduce levels of cross-infections caused by contaminated equipment. Descriptors: cross-infection; ventilators, mechanical; microbiological analysis; nursing, team; intensive care unit.RESUMOObjetivo: identificar a presença de bactérias patogênicas nos anexos do ventilador mecânico (VM), antes e após sua montagem, e discutir a descontaminação realizada pela equipe de enfermagem de um hospital regional. Método: trata-se de estudo transversal, do tipo descritivo exploratório, de natureza quantitativa. A coleta de dados foi realizada com swabs em cinco pontos específicos das partes internas dos anexos de ventilação mecânica, antes e após sua montagem, em uma unidade de terapia intensiva. O material coletado foi semeado em meios de cultura para microrganismos de importância médica e identificados em nível de gênero e espécie através de provas bioquímicas. Os resultados foram analisados com o programa SPSS 17.0. Resultados: antes da montagem dos anexos do VM, houve presença de 250 UFC/mm³ de Staphylococcus aureus; após a montagem dos anexos do VM, contabilizou-se 500 UFC/mm³ de Staphylococcus aureus, 250 UFC/mm³ de Enterococcus sp. e 1.000 UFC/mm³ de Enterobacter sp. Conclusão: houve falhas no processo de desinfecção, e, também, na manipulação dos anexos do VM, apresentando contaminação nos dois momentos estudados. Medidas de educação continuada para equipe de saúde, em especial para equipe de enfermagem, podem reduzir os índices de infecções hospitalares causadas por equipamentos contaminados. Descritores: infecção hospitalar; ventiladores mecânicos; análise microbiológica; equipe de enfermagem; unidade de terapia intensiva. RESUMENObjetivo: identificar la presencia de bacterias patogénicas en los accesorios del ventilador mecánico (VM) antes y después de su montaje, y discutir la descontaminación realizada por el equipo de enfermería de un hospital regional. Método: esto es un estudio transversal, descriptivo exploratorio, de naturaleza cuantitativa. La recogida de datos fue realizada con swabs en cinco puntos específicos de las partes internas de los accesorios de ventilación mecánica, antes y después de su montaje, en una unidad de terapia intensiva. El material recogido fue depositado en medios de cultura para microorganismos con importancia médica y identificados a nivel de género y especie a través de pruebas bioquímicas. Los resultados fueron analizados con el programa SPSS 17.0. Resultados: antes del montaje de los accesorios del VM, hubo presencia de 250 UFC/mm³  de Staphylococcus aureus; después del montaje de los accesorios del VM, se contó 500 UFC/mm³ de Staphylococcus aureus, 250 UFC/mm³ de Enterococcus sp. y 1.000 UFC/mm³ de Enterobacter sp. Conclusión: hubo fallas en el proceso de desinfección, y, también, en el manejo de los accesorios del VM, presentando contaminación en los dos momentos estudiados. Medidas de educación continuada para el personal de salud, en especial para el equipo de enfermería, pueden reducir los índices de infecciones hospitalarias causadas por equipamientos contaminados. Descriptores: infección hospitalaria; ventiladores mecánicos; análisis microbiológico; equipo de enfermería; unidad de terapia intensiva.

Mechanical ventilator a source of nosocomial infection

2011 ◽  
Vol 5 (2) ◽  
pp. 301
Author(s):  
Marquiony Marques Santos ◽  
Cristyanne Samara Miranda de Holanda ◽  
Izabel Calixta de Alcântara ◽  
Eudes Euler de Souza Lucena
Keyword(s):  
Intensive Care ◽  
Pathogenic Bacteria ◽  
Univariate Analysis ◽  
Microbiological Analysis ◽  
Mechanical Ventilator ◽  
Critical Material ◽  
Before And After ◽  
Microbiological Sample ◽  
Terapia Intensiva ◽  
The City

ABSTRACT Objectives: to verify the presence of pathogenic bacteria in the annexes of the mechanical ventilator, before and after its mounting in the Intensive Care Unit of Hospital Regional do Seridó in the city of Caicó-RN. Method: a descriptive and transversal study was carried out from a quantitative approach. The microbiological sample will be collected in the annexes of the mechanical ventilator. The sample will be taken to isolation procedures and identification. The data will be analyzed inSPSS 17.0 biostatistics software for the univariate analysis, by measuring the distribution center location variability and variability or dispersion, using Student´s t-test. Expected results: to verify the sterility of the semi-critical material of mechanical ventilator, prior to its use in patients in the hospital dynamics. In case of pathogenic microorganisms presence, through the study, to assess possible errors in the process (sterilization or mounting). The results of this work will contribute towards a reduction in hospitalization time, decrease in spending on hospital supplies and increased survival rate and the welfare of hospitalized. Descriptors: cross infection; ventilators, mechanical; microbiological analysis; nursing, team; intensive care units.RESUMOObjetivo: verificar a presença de bactérias patogênicas nos anexos do ventilador mecânico, antes e após sua montagem, na Unidade de Terapia Intensiva do Hospital Regional do Seridó, no município de Caicó-RN. Método: estudo do tipo descritivo exploratório de corte transversal, de natureza quantitativa. A coleta microbiológica do material será realizada nos anexos do ventilador mecânico. Em seguida, a amostra será levada para procedimentos de isolamento e identificação. Os dados serão analisados no software de bioestatística SPSS 17.0, para análise univariada, por meio de medidas do centro de distribuição de posição da variabilidade e variabilidade ou dispersão, utilizando o teste “t” de Student. Resultados esperados: Espera-se verificar a esterilidade do material semi-crítico do ventilador mecânico antes de seu uso no paciente na sua dinâmica hospitalar. Em caso de presença de microrganismo patogênico, através do estudo, avaliar os possíveis erros no processo (esterilização ou montagem). O resultado deste trabalho poderá contribuir para um programa de redução no tempo de internação, diminuição de gastos com materiais hospitalares e o aumento da taxa de sobrevida e o bem-estar dos usurários. Descritores: infecção hospitalar; ventiladores mecânicos; análise microbiológica; equipe de enfermagem; unidade de terapia intensiva.RESUMENObjetivo: determinar la presencia de bacterias patógenas en los anexos del ventilador mecánico (VM), antes y después de su montaje en la Unidad de Cuidados Intensivos del Hospital Regional del Seridó, en el municipio de Caicó-RN. Método: estudio descriptivo exploratorio de corte transversal, de naturaleza cuantitativa. El muestreo microbiológico del material se llevará a cabo en los anexos del ventilador mecánico. Luego, la muestra se tomará a los procedimientos de aislamiento e identificación. Analizaremos los datos en software de bioestadística SPSS 17.0. Análisis univariado, utilizando medidas del centro de distribución de posición de la variabilidad y la variabilidad o la dispersión, utilizando la prueba “t” de Student. Resultados esperados: Previstos para verificar la esterilidad del ventilador material semi-críticos antes de su uso en pacientes en la dinámica de su hospital. En caso de presencia de microorganismos patógenos, a través del estudio, para evaluar los posibles errores en el proceso (la esterilización o el montaje). El resultado de este trabajo contribuirá a una reducción de la duración de la estancia, disminuir los gastos de hospitalización de los suministros y la tasa de aumento de la supervivencia y el bienestar de los usureros. Descriptores: infección hospitalaria, ventiladores mecánicos; análisis microbiológico; grupo de enfermería, unidades de terapia intensiva.

scholarly journals Evaluation of the hygienic-sanitary conditions of a dairy localized in the Island of São Luís, Maranhão

2020 ◽  
Vol 87 ◽  
Author(s):  
Rildon Porto Candeira ◽  
Lenka de Morais Lacerda ◽  
Arlene dos Santos da Silva ◽  
Lygia Silva Galeno ◽  
Brenda Fernanda Sodré Moreno ◽  
...  
Keyword(s):  
Food Safety ◽  
Quality Management ◽  
Competitive Advantage ◽  
Food Industry ◽  
Quality Management System ◽  
Microbiological Analysis ◽  
Food Handlers ◽  
Sanitary Condition ◽  
Good Manufacturing Practices ◽  
Sanitary Conditions

ABSTRACT: The product quality is a competitive advantage that plays a differential role among companies. In the food industry, which is based on the Quality Management System, such as Good Manufacturing Practices (GMP), which cover the hygiene procedure, aiming at food safety. In view of the above, the aim of this study was to evaluate the hygienic-sanitary conditions of a selected dairy from the São Luís Island - MA. An application of the checklist was performed, swab collection from the hands of the manipulators and equipment and the collection of water and yogurt for microbiological analysis. After this step, a training was performed for food handlers and finally, new collections and microbiological analysis were performed. All the microbiological analysis performed were satisfactory, except for the water sample, one before and again for training. It can be verified that the hygienic-sanitary condition of the dairy was good. However, after a lecture and new microbiological analyzes, improvements were observed in the results.

scholarly journals Avaliação do conhecimento de manipuladores de alimentos após intervenções educativas em Palmas, Tocantins

2017 ◽  
Vol 4 (3) ◽  
pp. 27
Author(s):  
Dark Luzia dos Santos Neto ◽  
Karuane Saturnino da Silva Araújo ◽  
Sandra Maria Botelho Mariano ◽  
Erika Suellen dos Santos Vieira ◽  
Fellipe Camargo Ferreira Dias ◽  
...  
Keyword(s):  
Inservice Training ◽  
Foodborne Diseases ◽  
Food Handlers ◽  
Food Services ◽  
Food Handling ◽  
Before And After ◽  
Handling Practices ◽  
Transfer Of Information ◽  
Food Handling Practices ◽  
Good Food

A mão de obra capacitada em boas práticas de manipulação de alimentos é escassa e as deficiências na forma de executar o serviço são visíveis. O objetivo deste estudo foi avaliar o conhecimento dos manipuladores de alimentos (MA) de serviços de alimentação em Palmas – Tocantins antes e depois dos treinamentos em boas práticas de manipulação. O estudo foi realizado através da aplicação de um questionário, baseado na Resolução RDC n° 216/2004, a fim de verificar se houve a transferência de informações e conhecimentos para os MA em função da percentagem de respostas certas. Foi observado aumento na percentagem de respostas na escala excelente de 55% para 83%. De acordo com esses resultados, sugere-se a cobrança mais efetiva por parte dos órgãos fiscalizadores para que estabelecimentos que produzam alimentos tenham programas de treinamentos; que as capacitações aconteçam com uma maior frequência, para minimizar os riscos de contaminações e consequentemente a incidências de doenças veiculadas por alimentos.   Palavras-chave: Serviços de Alimentação, Capacitação em Serviço, Manipulação de Alimentos. ABSTRACT The skilled workforce in good food handling practices is scarce and deficiencies in the way the service is performed are visible. The objective of this study was to evaluate the knowledge of food handlers (FH) of food services in Palmas - Tocantins before and after training in good handling practices. The study was carried out through the application of a questionnaire, based on RDC Resolution 216/2004, in order to verify the transfer of information and knowledge to the FH according to the percentage of correct answers. An increase in the percentage of responses on the excellent scale from 55% to 83% was observed. According to these results, it is suggested the most effective collection by the enforcement agencies so that establishments that produce food have training programs; that capacities occur more frequently in order to minimize the risk of contamination and consequently the incidence of foodborne diseases. Keywords: Food Services, Inservice Training, Food Handling.

scholarly journals ATP-Bioluminescence and Conventional Microbiology for Hygiene Evaluation of Cutting Room Surfaces in Poultry Slaughterhouse.

2018 ◽  
Vol 46 (1) ◽  
pp. 6 ◽  
Author(s):  
Laura Beatriz Rodrigues ◽  
Luciana Ruschel Dos Santos ◽  
Natalie Nadin Rizzo ◽  
Daiane Ferreira ◽  
Amauri Picollo De Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Peracetic Acid ◽  
Hot Water ◽  
Economic Losses ◽  
E Coli ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Contact Surfaces ◽  
Sanitary Conditions

Background: The hygiene procedures in poultry slaughterhouses consist in the use of hot water, detergent and sanitizing, configuring Sanitation Standard Operating Procedure (SSOP). These actions control contamination in food processing environments, especially by pathogenic microorganisms, which cause diseases with impact on public health and economic losses. The microbiological control of aerobic mesophiles, Staphylococcus aureus and Escherichia coli, are used as indicators of contamination. The hygienic-sanitary conditions on the surfaces of the poultry slaughterhouse cuttting room were evaluated, before and after cleaning and sanitizing procedures.Materials, Methods & Results: Conventional microbiology (Rodac plates and sponge for quantification of aerobic mesophiles, Staphylococcus aureus and Escherichia coli) and ATP-Bioluminescence were used to analyze the action of hot water and the active principles peracetic acid, quaternary ammonia and biguanide in the standard pre-operational hygiene procedure in the cutting room of the poultry slaughterhouse under Federal Inspection with slaughter capacity of more than 20.000 birds/h. The evaluations were performed on three lines of chicken thigh cuts at the same time and in a completely randomized manner on stainless steel surfaces, polyurethane belts and polyethylene boards. Samples were made in four replicates at the three surface totaling 108 assay for each microorganism. The samples were collected at the end of the cuttingprocess, before and after washing the surfaces with hot water (between 45 and 50ºC) and after sanitization with 0.5% peracetic acid, 2% quaternary ammonia and 1% biguanide. The ATP-Bioluminescence method detected organic matter at all collected points and Rodac plates allowed a better recovery of microorganisms than sponges for quantification of aerobic mesophiles, E. coli and S. aureus. There was a reduction of contamination after the action of hot water and, after using quaternary ammonia and peracetic acid, there was no isolation of E. coli and S. aureus on all evaluated surfaces.Discussion: The use of different methods of analysis for monitoring the hygiene and sanitary status of contact surfaces with chicken cuts allows greater flexibility in relation to hygiene control. The use of the bioluminescent ATP detection method allows detecting in seconds extremely low levels of contamination, allowing a quick determination of the cleaning efficiency on the surfaces and evaluation of the hygiene programs. Conventional microbiology methods, on the other hand, provideindicators of contamination by different microorganisms on food contact surfaces. Both are applicable in SSOP monitoring programs and sanitary conditions of the contact surfaces in food producing establishments. The significant reduction of microorganisms on surfaces after cleaning, found in this study, demonstrates the importance of operational hygiene inthe maintenance of microbial contamination below the recommended limits, and to reconcile the ATP-Bioluminescence methodologies and Rodac plates can bring benefits to the control of this contamination, and the use of ATP-bioluminescence makes possible taking immediate corrective measures after the evaluation of sanitation procedures.Keywords: ATP-Bioluminescence, Rodac plates, Escherichia coli, mesophiles, Staphylococcus aureus.

scholarly journals ATP-Bioluminescence and Conventional Microbiology for Hygiene Evaluation of Cutting Room Surfaces in Poultry Slaughterhouse

2018 ◽  
Vol 46 (1) ◽  
Author(s):  
Laura Beatriz Rodrigues ◽  
Luciana Ruchel Dos Santos ◽  
Natalie Nadin Rizzo ◽  
Daiane Ferreira ◽  
Amauri Picollo De Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Peracetic Acid ◽  
Hot Water ◽  
Economic Losses ◽  
E Coli ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Contact Surfaces ◽  
Sanitary Conditions

  Background: The hygiene procedures in poultry slaughterhouses consist in the use of hot water, detergent and sanitizing, configuring Sanitation Standard Operating Procedure (SSOP). These actions control contamination in food processing environments, especially by pathogenic microorganisms, which cause diseases with impact on public health and economic losses. The microbiological control of aerobic mesophiles, Staphylococcus aureus and Escherichia coli, are used as indica­tors of contamination. The hygienic-sanitary conditions on the surfaces of the poultry slaughterhouse cuttting room were evaluated, before and after cleaning and sanitizing procedures.Materials, Methods & Results: Conventional microbiology (Rodac plates and sponge for quantification of aerobic me­sophiles, Staphylococcus aureus and Escherichia coli) and ATP-Bioluminescence were used to analyze the action of hot water and the active principles peracetic acid, quaternary ammonia and biguanide in the standard pre-operational hygiene procedure in the cutting room of the poultry slaughterhouse under Federal Inspection with slaughter capacity of more than 20.000 birds/h. The evaluations were performed on three lines of chicken thigh cuts at the same time and in a completely randomized manner on stainless steel surfaces, polyurethane belts and polyethylene boards. Samples were made in four replicates at the three surface totaling 108 assay for each microorganism. The samples were collected at the end of the cut­ting process, before and after washing the surfaces with hot water (between 45 and 50ºC) and after sanitization with 0.5% peracetic acid, 2% quaternary ammonia and 1% biguanide. The ATP-Bioluminescence method detected organic matter at all collected points and Rodac plates allowed a better recovery of microorganisms than sponges for quantification of aerobic mesophiles, E. coli and S. aureus. There was a reduction of contamination after the action of hot water and, after using quaternary ammonia and peracetic acid, there was no isolation of E. coli and S. aureus on all evaluated surfaces.Discussion: The use of different methods of analysis for monitoring the hygiene and sanitary status of contact surfaces with chicken cuts allows greater flexibility in relation to hygiene control. The use of the bioluminescent ATP detection method allows detecting in seconds extremely low levels of contamination, allowing a quick determination of the cleaning efficiency on the surfaces and evaluation of the hygiene programs. Conventional microbiology methods, on the other hand, provide indicators of contamination by different microorganisms on food contact surfaces. Both are applicable in SSOP monitor­ing programs and sanitary conditions of the contact surfaces in food producing establishments. The significant reduction of microorganisms on surfaces after cleaning, found in this study, demonstrates the importance of operational hygiene in the maintenance of microbial contamination below the recommended limits, and to reconcile the ATP-Bioluminescence methodologies and Rodac plates can bring benefits to the control of this contamination, and the use of ATP-bioluminescence makes possible taking immediate corrective measures after the evaluation of sanitation procedures.Keywords: ATP-Bioluminescence, Rodac plates, Escherichia coli, mesophiles, Staphylococcus aureus.

scholarly journals Zadżumione miasto. Sztokholm wobec zarazy w latach 1710–1711

2021 ◽  
Vol 12 (2) ◽  
pp. 228-246
Author(s):  
Gabriela Majewska
Keyword(s):  
High Rate ◽  
Peter The Great ◽  
Rapid Spread ◽  
Travel Restrictions ◽  
History Of ◽  
Set Up ◽  
The Baltic ◽  
Sanitary Conditions ◽  
The City ◽  
Compulsory Registration

Plague city. Stockholm and the plague of 1710 The epidemic of plague in Stockholm between 1710 and 1711 was part of a great pandemic that devastated Eastern, Central, and Northern Europe in the first decade of the century and, at the same time, was the greatest demographic catastrophe in the history of the Swedish capital, as a result of which around 40% of the city’s population died. The first cases of plague appeared at the start of 1710; the peak of infections and deaths was in the autumn (October and November); in winter, the disease lessened and the last cases occurred in February 1711. It is estimated that the number of deaths reached 22,000. The suburbs and the poorest districts suffered most. Scarcity and famine, which struck Sweden at the end of the first decade of the XVIIIth century, fostered the rapid spread of the disease in the city and the high rate of mortality. The plague was also fostered by the effects of the war conducted by Charles XII and, particularly, the defeats suffered by the Swedish army and the seizure of the Baltic provinces by Peter the Great, which led to migrations from already plague-infected regions and worsened the already poor sanitary conditions in the city. Refugees from Estonia probably brought the plague to Stockholm. The Swedish authorities undertook measures against any epidemic as early as 1708 after receiving information about the spread of plague on the northern shores of the Baltic. These were limited to monitoring the movement of population. In 1709, quarantine was introduced for arrivals. After the first cases of plague in Stockholm regulations concerning people’s movements were made more strict, epidemic services were set up, and sanitary and public order directives were issued. Announcement of the plague was delayed until mid-September, when the number of deaths rose hugely, and it was obvious that the authorities were not in a position to control the spread of the epidemic. The city was closed, an ordinance against plague was issued regulating especially issues connected with health certificates, travel restrictions, maintaining cleanliness in houses and on the streets, compulsory registration and isolation of the sick, organization of interments, and the use of disinfectants and medicines. The reaction of the Stockholm authorities to the appearance of plague in the city reflected the actions of other Baltic cities struggling with plague at the same time.

Staphylococcus aureus nasal colonization among Vietnamese adults: prevalence, risk factors and antibiotic susceptibility profile

MedPharmRes ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 21-31
Author(s):  
Nguyen Phan ◽  
Hien Pham ◽  
Thuc Nguyen ◽  
Hoai Nguyen
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Nasal Carriage ◽  
Resistance Rate ◽  
High Rate ◽  
Weight History ◽  
Potential Risk Factors ◽  
History Of ◽  
Vietnamese Community

Staphylococcus aureus (S. aureus) has long been recognized as an important human pathogen causing many severe diseases. It is also a part of human normal flora with its ecological niche in the human anterior nares. This study focused on screening S. aureus nasal carriage in community and its relationship to human physiological and pathological factors which have not been studied in Vietnam previously. Two hundred and five volunteers in Ho Chi Minh City from 18 to 35 and over 59 years old both male and female participated in the study. Result showed that the prevalence of S. aureus nasal carriage in southern Vietnamese community was relatively low, only 11.2% (23/205), much lower than that in other international reports on human S. aureus. In addition, nasal carriage of the older age group (> 59 years old, 13.7%) was higher than that of younger age (18-35 years old, 10.4%). Other potential risk factors such as gender, career, height, weight, history of antibiotic usage, daily nasal wash, use of nasal medication sprays, acne problems, smoking and nasal problems showed no significant impact on S. aureus carriage. The obtained S. aureus nasal isolates were all sensitive to vancomycin. Lincomycin and tetracycline had low resistance rate with 4.3 % and 17.4 %, respectively. However, the isolates showed particularly high rate of multidrug resistance (54.2%) In summary, our data provided researchers an overview on S. aureus nasal carriage and antibiotic susceptibility profile of the community- isolated S. aureus in Vietnam. This would serve as valuable information on assessing risk of community-acquired S. aureus infections.

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