scholarly journals Efektifitas Ekstrak Etanol Daun Brotowali Sebagai Insektisida Terhadap Nyamuk Aedes aegypti di Universitas Abdurrab Pekanbaru

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Siti Juariah ◽  
Sri Kartini ◽  
Yulinda Wardani
Keyword(s):  
Aedes Aegypti ◽  
Control Group

Aedes aegypti merupakan jenis nyamuk yang berperan sebagai vektor virus Dengue. Selama ini, pengendalian vektor virus tersebut dilakukan menggunakan insektisida kimiawi. Namun ternyata jika dilakukan secara terus menerus, akan menyebabkan timbulnya resistensi sehingga diperlukan suatu upaya untuk menangani hal tersebut. Salah satu cara yang kemungkinan dapat dilakukan yaitu menggunakan insektisida alami yang berasal dari tumbuh- tumbuhan. Salah satu tanaman yan dianggap dapat digunakan sebagai insektisida alami yaitu ekstrak daun brotowali (Tinospora crispa L). Tujuan penelitian ini adalah untuk mengetahui efektifitas ekstrak etanol daun brotowali (Tinospora crispa L) sebagai insektisida terhadap nyamuk Aedes aegypti. Penelitian ini menggunakan metode eksperimen laboratorium dengan desain penelitian design post only control group. Ekstrak etanol daun brotowali dibuat dalam berbagai konsentrasi yaitu 15%, 20%, dan 25% kemudian dilakukan penyemprotan terhadap nyamuk Aedes aegypti diamati selama 60 menit. Hasil penelitian menunjukkan bahwa konsentrasi 15%, 20% dan 25% dapat membunuh nyamuk secara berturut-turut yaitu sebanyak 1 ekor (20%) , 3 ekor (60%), dan 4 ekor (80%). Hasil penelitian ini dapat disimpulkan bahwa ekstrak etanol daun brotowali (Tinospora crispa L) dapat digunakan sebagi insektisida terhadap nyamuk Aedes aegypti.

scholarly journals The Effect Of Bioinsecticide Granule Mixture Of Sugar Apple Seed (Annonae Squamosa) And Betel Leaf (Piper Betle) On Morphology Of Eggs Aedes Aegypti

2021 ◽  
Vol 9 (1) ◽  
pp. 26-30
Author(s):  
Firdha Firdha Novitasari ◽  
Dwi Wahyuni Dwi Wahyuni ◽  
Erma Sulistyaningsih Erma Sulistyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Public Health Problem ◽  
Ordinal Regression ◽  
Seed Extract ◽  
Control Group ◽  
Piper Betle ◽  
Betel Leaf ◽  
Apple Seed ◽  
Regression Test ◽  
Apple Seeds

Introduction: Dengue hemorrhagic fever (DHF) is an important public health problem in Indonesia because its morbidity and mortality are quite high. Prevention by vector control methods with chemical insecticides adversely affects the environment. Research on granular bioinsectide mixture of betel leaf extract and sugar apple seeds against Aedes aegypti is expected to be used to break the chain of Aedes aegypti mosquitoes starting  from the juvenile stage  (eggs) as an effort to control the vector population naturally. Objective: This study aims to analyze the effect of granule bioinsecticide mixture of sugar apple seed extract and betel leaf on changes in the morphology of Aedes aegypti eggs. Methods: This study is a true-experimental study with a Post test Only Control Group Design design. The treatment group will be given a granular bioinsecticide, a mixture of sugar apple seed extract and betel leaf, positive control will be given abate while negative control will be given aquades. Each group uses 25 eggs with 3 repetitions. On day 3, all eggs will be observed using a stereo microscope to determine morphological changes. The data obtained will be analyzed using ordinal regression test. Results: the ordinal regression test got the value of ρ in the Wald test between (0.012 - 0.955)> α = 0.01. Conclusion: there is no effect of mixed granules of betel leaf and sugar apple seeds on the mortality of Aedes aegypti eggs. Further research is needed on new formulations using other natural ingredients to increase the effect of ovicides on Aedes aegypti.

scholarly journals Status Kerentanan Aedes Aegypti Vektor Demam Berdarah Dengue di Kota Padang

2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.

scholarly journals Mosquito Larvacidal Activity of Zingiber montanum Rhizome Extract Against Aedes aegypti larvae

2019 ◽  
pp. 33-40
Author(s):  
Dwi Sulistia Ningrum ◽  
Siwi Pramatama Mars Wijayanti ◽  
Kuswanto Kuswanto
Keyword(s):  
Aedes Aegypti ◽  
Negative Impact ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Larvicidal Effect ◽  
Natural Insecticide ◽  
Post Test ◽  
Quasi Experimental ◽  
Lc50 And Lc90

Aedes aegypti is the main vector which transmits Dengue virus as causing agent of Dengue Haemmorhagic Fever (DHF). Chemical control of Ae. aegypti have an impact on the environment and humans, also burden a high cost. One of the efforts to reduce the negative impact of synthetic insecticide, which is to find out alternative natural insecticide from plant-based insecticides. The purpose of this research is to determine the killing power of the rhizome bangle extract to Ae. aegypti larvae. This research was a quasi-experimental design with post test only control group design. The concentration of extract rhizome bangle used were 0%; 0.125%; 0.25%; 0.5%; 0.75% and 1%. The mosquito sample used in this study were instar III of Ae. aegypti larvae as many as 600 larvae. Data analysis used univariate and bivariate (probit and Kruskal Wallis test). The results showed that there was an effect of the concentration of extract rhizome bangle against the mortality of Ae. aegypti larvae with p=0,002 (p<0,05). Extract rhizome bangle effectively killed Ae. aegypti larvae with LC50 and LC90 were 0.148 % and 0.338 %, with the most effective concentration is 1%. Based on this research, extract rhizome bangle has a larvicidal effect on Ae. aegypti, so it can be used as an alternative method to minimize the usage of chemical larvicides that easily applied by the community.

scholarly journals EFEKTIVITAS EKSTRAK DAUN KEMANGI (Ocimum sanctum) SEBAGAI BIOLARVASIDA TERHADAP KEMATIAN LARVA Aedes aegypti TAHUN 2017

2018 ◽  
Vol 37 (4) ◽  
pp. 506-511
Author(s):  
Febrina Dyta Pravitri ◽  
Khomsatun Khomsatun
Keyword(s):  
Aedes Aegypti ◽  
Ocimum Sanctum ◽  
Control Group

AbstrakPenyakit Demam Berdarah Dengue salah satu penyakit endemis yang dapat menimbulkan wabah.Salahsatu upaya yang penting adalah memutus rantai penularan, yaitu dengan menggunakaninsektisida.Pengendalian vektor DBD umumnya menggunakan insektisida kimia yang berdampak negatifterhadap lingkungan.Penelitian ini dilakukan untukmengetahui efektivitas pengaruh berbagai konsentrasiekstrak daun kemangi (Ocimum sanctum) terhadap kematian larva Aedes aegypti.Metode penelitian iniadalah quasi exsperiment dengan rancangan the posttest-only control group untuk menghitung kematianlarva Aedes aegypti pada konsentrasi 0,3%, 0,9% dan 15% ekstrak daun kemangi. Konsentrasi tersebutdimasukkan kedalam enamel berukuran 250 ml yang masing-masing berisi 25 ekor larva Aedes aegypti,diamati pada jam ke 1, 2, 3, 4, 5, 6 dan 24 jam.Hasil penelitian menunjukkan bahwa ada perbedaanberbagai konsentrasi ekstrak daun kemangi (Ocimum sanctum) dalam mematikan larva Aedes aegypti,untuk konsentrasi 0,3% adalah 7 ekor (26,4%), konsentrasi 0,9% adalah 14 ekor (54,4%) dan konsentrasi1,5% adalah 20 ekor (81,2%).Simpulan penelitian ini adalah ekstrak daun kemangi dengan berbagaikonsentrasi yang digunakan belum efektif karena belum mampu membunuh larva Aedes aegypti sampai90% selama 24 jam.Disarankan untuk peneliti selanjutnya dapat menggunakan konsentrasi ekstrak daunkemangi yang lebih tinggi.

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals Effectiveness of Kelor Dung Extract on Aedes aegypti Larvae Mortality

2018 ◽  
pp. 10-15
Author(s):  
Alfina Baharuddin
Keyword(s):  
Aedes Aegypti ◽  
Exposure Time ◽  
Design Method ◽  
Narrative Form ◽  
Control Group ◽  
Control Group Design ◽  
Control Effort ◽  
Average Percentage ◽  
Group Design ◽  
Post Test

The high number of cases of dengue fever in Indonesia, so it needs to be held vector control effort. Moringa is used as a natural anti-mosquito capable of supplying larvae. This is because there are compounds of steroids, triterpenoids, alkaloids, saponins, flavanoids, and tannins in the stem of moringa. The type of research used was experimental with post test only control group design method. The data were collected by counting the number of Aedes aegypti instar III larvae that died in each type of salinity concentration during the exposure time. The experiment was conducted 4 times, Observation, calculation and recording of the number of live and dead larvae every 24, 32, 40 and 48 hours after treatment. The data that has been collected and analyzed is then presented in tabular and narrative form. At the concentration of 3% in the Moringa leather bark estrants at the time of 24 hour measurements in each treatment of 1 (3), 2, 3, 3 (3) treatment, 4 (1) treatment with an average percentage of larva death 27 , 5%. The concentration of 3% extract of Moringa leaf bark effectively kills Aedes aegepty larvae by 45% within 48 hours. Concentration of 3.5% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 45% within 24 hours. The concentration of 4% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 67.5% within 24 hours. The effective exposure time of 24 hours resulted in the death of 67.5% Aedes aegypti.  

scholarly journals Efektivitas Ekstrak Biji Bintaro (Cerbera manghas) sebagai Larvasida Hayati pada Larva Aedes aegypti Instar III

2018 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Khoiriyanti Wulandari ◽  
Mei Ahyanti
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Contact Time ◽  
Statistical Tests ◽  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Cerbera Manghas

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>

scholarly journals MORPHOLOGICAL AND HISTOLOGICAL EFFECTS OF BRUCEINE A ON THE LARVAE OF AEDES AEGYPTI LINNAEUS (DIPTERA: CULICIDAE)

2018 ◽  
Vol 11 (10) ◽  
pp. 422
Author(s):  
Dwi Sutiningsih ◽  
Mustofa Mustofa ◽  
Tri Baskoro Tunggul Satoto ◽  
Edhi Martono
Keyword(s):  
Epithelial Cells ◽  
Aedes Aegypti ◽  
Histological Change ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Histological Changes ◽  
Brucea Javanica ◽  
Gastrointestinal Damage

Objective: This study aimed to determine a target of action of bruceine A on the basis of its morphological and histological effects on the larvae of Aedes aegypti Linnaeus.Methods: Bruceine A was isolated from Brucea javanica (L.) Merr. seeds in accordance with the Mangungsong method. Larvae of A. Aegypti (L.) in instar III to the beginning of instar IV were treated with various concentrations of bruceine A. The negative control group did not receive any treatment, whereas the positive control group received 1 ppm temefos. Dead larvae were collected after 24 h of treatment for the examination of morphological and histological changes.Results: The negative control group did not exhibit any morphological and histological changes. Larvae treated with bruceine A, however, had visible damaged heads, cuticles, digestive and respiration tracts, respiratory siphons, and setae, and they were smaller than normal larvae. Larvae treated with temefos exhibited gastrointestinal damage, narrowed breathing tubes, cuticle damage, and detached/damaged seta feathers. The necrosis of gastrointestinal epithelial cells was the major histological change exhibited by larvae treated with various concentrations of bruceine A or 1 ppm temefos.Conclusion: The targets of action of bruceine A in A. aegypti (L.) larvae are the head/caput, cuticle, setae, siphon, and gastrointestinal and respiratory tracts.

scholarly journals RELATIONSHIPS OF THE IMPLEMENTATION OF A 3M PLUS PROGRAM AND THE EXISTENCE OF AEDES AEGYPTI LARVAE TOWARDS THE NUMBER OF DENGUE FEVER CASES IN THE PUBLIC HEALTH CENTER OF TIGO BALEH BUKITTINGGI, WEST SUMATERA, INDONESIA

2017 ◽  
Vol 3 (3) ◽  
pp. 131-137
Author(s):  
Syukra Alhamda
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Health Center ◽  
Dengue Fever ◽  
Control Group ◽  
Case Group ◽  
Chi Square ◽  
Public Health Center ◽  
The Public ◽  
Index Value

Background: Dengue fever in Tigo baleh Bukittinggi community is one of the infectious diseases resulting in serious health problems. Understanding the factors associated with the disease is necessity.Objective: To determine the relationships of the implementation of 3M plus program and the existence of Aedes aegypti larvae towards the number of dengue fever cases in the public health center of Tigo Baleh Bukittinggi, West Sumatera, Indonesia.Methods: This was a case control study conducted between January and December 2016 in the Public Health Center of Tigo Baleh Bukittinggi. There were 78 samples recruited by total sampling, with 39 were assigned in the case and control group. House index (HI) and Container index (CI) were used to measure the presence of Aedes aegypti larvae. The implementation of 3M plus program was measured using questionnaire developed by the researcher. Chi Square was performed for data analysis. Result: Findings showed that house index value was 43.6% and container index was 17.9%. The presence of larvae at home contributes to 24 (61.5%) cases of DHF in the case group and 10 (25.6%) cases in the control group. Poor implementation of the program contributes to 27 (69.2%) cases in the case group and 17 (43.6%) in the control group. Chi square test showed p 0.040 (0.05) for the 3M plus implementation and p 0.03 (0.05) for the presence of Aedes aegypti. Conclusion: There were significant associations between the implementation of 3m plus program and the existence of Aedes aegypti larvae towards the number of dengue fever cases in the public health center of Tigo Baleh Bukittinggi, West Sumatera, Indonesia. It is suggested that the community should do more efforts to prevent the occurrence of DHF through mosquito nest eradication, such as the implementation of 3M Plus and sanitation efforts, and pay attention to any places that potentially become a breeding ground for Aedes aegypti mosquitoes.

scholarly journals The Effect of Sugar Apple (Annona squamosa L.) Seeds Solution as an Insecticide Against Mortality of Aedes aegypti Mosquito with Liquid Electric Method

2018 ◽  
Vol 9 (2) ◽  
pp. 200
Author(s):  
Amalinda Mega Novasari ◽  
Retno Sasongkowati
Keyword(s):  
Aedes Aegypti ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Cross Sectional Study ◽  
Primary Data ◽  
Control Group ◽  
Annona Squamosa ◽  
Control Group Design ◽  
Cross Sectional ◽  
Apple Seeds

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.

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