PENGARUH KONSENTRASI AIR REBUSAN DAUN MELINJO (Gnetum Gnemon L.) TERHADAP TOTAL MIKROBA KERABANG DAN SPECIFIC GRAVITY TELUR AYAM RAS

AbstractThis study aimed to determine the effect of soaking broiler eggs into boiled water of melinjo (Gnetum gnemon L.) leaves with different concentrations on the total microbial shell and Specific Gravity of broiler eggs. This research was conducted at the Laboratory of Animal Husbandry, Department of Animal Husbandry, University of Bengkulu and UPTD Laboratory of Veterinary Public Health at the Department of Animal Husbandry and Animal Health Bengkulu Province. The research used was a completely randomized design (CRD) with 4 treatments and each design consisted of 4 replications. P0: Control chicken eggs without soaking in boiled water of melinjo leaves (ARDM). P1: (ARDM) 15%. P2: (ARDM) 30%. P3: (ARDM) 45%. The variables observed in this study were the percentage change in egg weight, the percentage of egg shell weight, specific gravity and TPC (Total Plate Count) of broiler eggs. The results showed that broiler eggs soaked with boiled water from melinjo leaves at a concentration of 15% - 45% had a significant effect (P<0.05) on the difference in the proportion of egg weight on the 21st day. However, there was no significant effect (P>0.05) on the proportion of shell weight, specific gravity and TPC (Total Plate Count) of broiler eggshells. Immersion of broiler eggs using boiled water of melinjo leaves was not able to reduce the number of egg shells, but was able to distinguish differences in egg weight, shell weight and could produce 21 days of storage compared to eggs without air soaking boiled melinjo leaves (control). Keywords: broiler eggs, melinjo leaves, Specific gravity, TPC (Total Plate Count)

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Prevalence of Microbial Contamination and Antibiotic Residue in Chicken Meat, Beef, and Offal in South Sulawesi

Hasanuddin Journal of Animal Science (HAJAS) ◽

10.20956/hajas.v1i1.6649 ◽

2019 ◽

Vol 1 (1) ◽

pp. 1-11

Author(s):

Sriyanti Haruni ◽

Ratmawati Malaka ◽

Hikmah M. Ali

Keyword(s):

Animal Health ◽

Animal Husbandry ◽

Positive Sample ◽

Chicken Meat ◽

Quality Analysis ◽

Plate Count ◽

Meat Sample ◽

South Sulawesi ◽

Total Plate Count ◽

Antibiotic Residue

Chicken meat, beef, and offal are important animal products. They easily spoiled due to bacterial contamination and might contain antibacterial residues derived from treatments during rearing and processing. This present work aimed to investigate location- and time-dependent value of TPC (Total Plate Count), and analyze antibiotic residue present in chicken meat, beef, and offal sampled in the Province of South Sulawesi. This current study was conducted by analyzing data released from UPT. Animal Product Quality Analysis in Department of Animal Husbandry and Animal Health, Province of South Sulawesi. Our experimental design was arranged according to completely randomized design with factorial (4×3), and carried out 5 replications. The results showed that two locations (Takalar District and Palopo District) had the lowest TPC value, while Palopo City possessed the highest TPC value. Regarding to antibiotic residue, the positive sample was only found in 2015, a chicken meat sample collected in Luwu Utara District.Keywords : Total Plate Count (TPC), Antibiotic Residues.

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KAJIAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT PISANG RAJA (Musa Paradisiaca var. Raja) DAN SERAI (Cymbopogon Citratus) TERHADAP UMUR SIMPAN BAKSO AYAM

Jurnal Sains dan Teknologi Pangan ◽

10.33772/jstp.v6i6.22812 ◽

2022 ◽

Vol 6 (6) ◽

Author(s):

Feren Feren ◽

Sri Wahyuni ◽

RH Fitri Faradilla

Keyword(s):

Storage Time ◽

Design Method ◽

Storage Period ◽

Control Treatment ◽

Plate Count ◽

Banana Peel ◽

Total Plate Count ◽

Organoleptic Characteristics ◽

Randomized Design ◽

Peel Extract

ABSTRACT The purpose of this study was to determine the interaction effect of storage time and concentration of plantain peel extract and lemongrass on organoleptic characteristics (color, aroma, texture, and elasticity) and total microbes in chicken meatballs. This study used a 2-Factorial Completely Randomized Design method. The first factor was storage time with three levels, namely 0 days (T0), 2 days (T1), and 4 days (T2. Meanwhile, the second factor was the concentration of plantain peel and lemongrass extract with three levels, namely 0% (C0), 30% plantain peel extract + 20% lemongrass extract (C1), 20% banana peel extract + 30% lemongrass extract (C2), and 25% banana peel extract + 25% lemongrass extract (C3). Observation variables were organoleptic tests which included color, aroma, texture, elasticity, and shelf life after treatment based on the results of the total plate count (TPC) calculation. The results show that the 2-day storage period with the addition of 25% plantain peel extract and 25% lemongrass extract (T1C3) was the best treatment for organoleptic characteristics (color, aroma, texture, and elasticity). Based on the results of the study, it can be concluded that the administration of plantain peel extract and lemongrass was able to maintain the quality of chicken meatballs for two different days compared to the control. Treatment with a storage period of 2 days with 25% plantain peel extract and 25% lemongrass extract (T1C3) was the best treatment that was most favored by the panelists and had the least number of microbial colonies.Keywords: Meatballs, preservatives, banana peel extract and lemongrass, storage time.ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh interaksi lama penyimpanan dan konsentrasi ekstrak kulit pisang raja dan serai terhadap uji organoleptik (warna, aroma, tekstur dan kekenyalan) dan total mikroba pada bakso ayam. Penelitian ini menggunakan metode Rancangan Acak Lengkap Faktorial 2 Faktor. Faktor Pertama adalah lama penyimpanan yang terdiri dari tiga taraf yaitu 0 hari (T0), 2 hari (T1) dan 4 hari (T2), faktor kedua yaitu perlakuan ekstrak kulit pisang raja yang terdiri atas tiga taraf yaitu konsentrasi 0% (C0), konsentrasi ekstrak kulit pisang raja 30% + konsentrasi ekstrak serai 20% (C1), konsentrasi ekstrak kulit pisang raja 20% + konsentrasi ekstrak serai 30% (C2), konsentrasi ekstrak kulit pisang raja 25% + konsentrasi serai 25% (C3). Variabel pengamatan yaitu uji organoleptik yang meliputi warna, aroma, tekstur, kekenyalan dan daya simpan setelah perlakuan berdasarkan hasil perhitungan jumlah total plate count (TPC). Hasil penelitian menunjukkan bahwa perlakuan lama penyimpanan 2 hari dengan penambahan ekstrak kulit pisang raja 25% dab serai 25% (T1C3) merupakan perlakuan terbaik untuk uji organoleptik (warna, aroma, tekstur dan kekenyalan). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian ekstrak kulit pisang raja dan serai mampu mempertahankan kualitas bakso ayam selama dua hari yang berbeda dengan kontrol. Perlakuan dengan lama penyimpanan 2 hari dengan pemberianekstrak kulit pisang raja 25% dan serai 25% (T1C3) merupakan perlakuan terbaik yang paling disukai oleh panelis dan memiliki jumlah koloni mikroba paling sedikit.Kata kunci: Bakso, pengawet, ekstrak kulit pisang dan serai, lama penyimpanan

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Efek Agitasi Susu Probiotik yang ditambahkan Buah Naga Merah (Hylocereus polyrhizus) terhadap Uji Sensorik dan Total Plate Count

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v1i1.1169 ◽

2016 ◽

Vol 1 (1) ◽

pp. 816-823

Author(s):

Feby Zalika Parindra ◽

Yusdar Zakaria ◽

Yurliasni Yurliasni

Keyword(s):

Ph Value ◽

Plate Count ◽

Milk Processing ◽

Total Plate Count ◽

Randomized Design ◽

Dragon Fruit ◽

Microbial P ◽

Two Factors ◽

Completely Randomized Design ◽

Hylocereus Polyrhizus

Abstrak. Suatu penelitian telah dilakukan untuk mengetahui efek agitasi susu probiotik yang ditambahkan buah naga merah (Hylocereus Polyrhizus). Penelitian ini dilakukan di Laboratorium Ilmu dan Teknologi Pengolahan Susu Fakultas Pertanian Universitas Syiah Kuala pada tanggal 29 April - 31 Mei 2016. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4 dengan 3 kali ulangan yang terdiri dari 2 faktor, A, agitasi yaitu (A1) dan (A2) non agitasi. Dan faktor B yaitu konsentrasi buah naga merah (B1) 20%, (B2) 25%, B3 (30%) dan (B4) 35%. Parameter yang diamati adalah derajat keasaman (pH), total mikroba dan uji organoleptik. Hasil penelitian menunujukkan bahwa terdapat pengaruh yang sangat nyata (P0,01) terhadap derajat keasaman (pH) dan berpengaruh nyata terhadap total mikroba (P0,05) serta terdapat interaksi antara kedua perlakuan. Sedangkan untuk uji organoleptik terdapat pengaruh yang sangat nyata (P0,01) terhadap kekentalan dan warna susu probiotik. nilai derajat keasaman pH 4,03 - 4,11 dengan agitasi (pengadukan) masih sesuai dengan standar (SNI) 01-2981-1992. Sedangkan nilai total mikroba optimal terjadi pada penambahan buah naga sebanyak 30% baik agitasi maupun non agitasi. Effects of Milk Probiotic Agitation Added Red Dragon Fruit (Hylocereus Polyrhizus) on Sensory Test and Total Plate Coun Abstract. A study about effects of milk probiotic agitation added red dragon fruit (Hylocereus Polyrhizus) has been conducted to determine to test sensory and total plate count. This research was held at the Laboratory of Science and Milk Processing Technology, Agriculture Fakulty Syiah Kuala University on April 29 to May 31, 2016. This study used a completely randomized design (CRD) 2x4 factorial design with three replications consisting of two factors, A, agitation (A1) and (A2) non agitation. And factor B is the concentration of red dragon fruit (B1) 20%, (B2) 25%, B3 (30%) and (B4) 35%. The observed parameter is the degree of acidity (pH), total microbial and organoleptic tests. The results of the study indicate that the there is a highly significant influence (P 0,01) to the degree of acidity (pH) and total significantly affected the total microbial (P 0.05), and there is interaction between the two treatments. As for organoleptic tests are highly significant effect (P 0.01) the thickness and color of probiotic milk. acidity pH value of 4.03 to 4.11 with agitation (stirring) is still in accordance with the standard (SNI) 01-2981-1992. While the total value of optimal microbial occurred upon addition of dragon fruit by 30% both agitation and non agitation

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Egg quality of laying hens fed diets with plant extracts

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v41i1.43801 ◽

2019 ◽

Vol 41 (1) ◽

pp. 43801

Author(s):

Debora Duarte Moraleco ◽

Jean Kaique Valentim ◽

Larissa Gonçalves Silva ◽

Heder José D'Ávila Lima ◽

Tatiana Marques Bitencourtt ◽

...

Keyword(s):

Specific Gravity ◽

Storage Time ◽

Laying Hens ◽

Egg Quality ◽

Shell Weight ◽

Flower Extract ◽

Randomized Design ◽

And Storage ◽

Haugh Unit

The objective of this study was to evaluate the effect of marigold flower extract and paprika on egg quality of Black Avifran laying hens in different periods of storage. A completely randomized design study with a 3x3 factorial scheme with three diets (control, paprika, and marigold flower extract) and three storage periods (0, 7, and 14 days) was carried out. The following metrics were evaluated: egg weight, yolk weight, shell weight; yolk color, albumen height, specific gravity, and Haugh unit as well as the yolk, shell, and albumen percentage relative to the total weight of the egg. There was no significant interaction between additives and storage time (p > 0.05) for most of the parameters evaluated. The only exception was observed for yolk color, in which the interaction effect between treatments was significant (p < 0.05). The effect of storage time was significant (p < 0.05) for albumen weight, albumen percentage, yolk color, albumen height, specific gravity, and Haugh unit, with reduction of these parameters over the evaluation period. The use of paprika and marigold flower extract in the diets of Black Avifran laying hens does not influence egg quality.

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Estimation of Shell Weight and Shell Thickness from Egg Specific Gravity and Egg Weight

Poultry Science ◽

10.3382/ps.0611991 ◽

1982 ◽

Vol 61 (10) ◽

pp. 1991-1995 ◽

Cited By ~ 30

Author(s):

JON O. NORDSTROM ◽

L.E. OUSTERHOUT

Keyword(s):

Specific Gravity ◽

Shell Thickness ◽

Shell Weight ◽

Egg Weight

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Pengaruh Penggunaan Ekstrak Kunyit (Curcuma domestica) Terhadap Mutu Kerupuk Cumi (Loligo sp.) [Effect of Using Turmeric Extract (Curcuma domestica) on The Quality of Squid Crackers (Loligo sp.)]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v11i1.11914 ◽

2019 ◽

Vol 11 (1) ◽

pp. 55

Author(s):

Jumiati Jumiati ◽

Dewi Ratnasari ◽

Achmad Sudianto

Keyword(s):

Protein Content ◽

Nutrient Content ◽

Proximate Analysis ◽

Plate Count ◽

Test Analysis ◽

Total Plate Count ◽

Turmeric Extract ◽

Randomized Design ◽

Completely Randomized Design

AbstrakKerupuk cumi saat ini cukup banyak diminati masyarakat di semua kalangan karena kandungan gizi cumi yang tinggi terutama kandungan proteinnya yaitu 17,9 g/100 g cumi segar. Penelitian ini bertujuan untuk mengetahui mutu kerupuk cumi (Loligo sp.) yang terbaik dengan penambahankunyit(Curcuma domestica) yang berbeda. Analisis yang dilakukan meliputi: analisa proksimat (kadar : air, abu, lemak, protein, dan karbohidrat), dan uji Total Plate Count (TPC Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak Lengkap (RAL), dengan empat perlakuanyaitu: tanpa pemberian ekstrak kunyit (O) , pemberian dosis 12,5% (A), 15% (B) dan 17,5% (C) masing-masing enam ulangan. Hasil analisa uji proksimat, penggunaan ekstrak kunyit pada pembuatan kerupuk cumi berpengaruh nyata terhadap mutu kerupuk cumi dengan pemakaian ekstrak kunyit terbaik yaitu: dosis kunyit 12,5% (kadar air : 9,622%), dosis kunyit 17,5% ( kadar lemak: 4,765%), dosis kunyit 17,5% (kadar protein : 18,112%), dosis kunyit 12,5% (kadar karbohidrat : 68,253%), dan dosis kunyit 12,5% (kadar abu: 1,278%). Penggunaan ekstrak kunyit berpengaruh sangat nyata terhadap TPC pada kerupuk cumi, perlakuan yang paling baik adalah perlakuan C dengan nilai TPC sebesar 28.350 koloni/ gram. Penggunaan esktrak kunyit sebagai bahan anti bakteri pada proses pembuatan kerupuk cumi mampu menghambat aktivitas bakteri dan mempunyai kandungan gizi yang cukup tinggi. AbstractSquid crackers are currently quite popular with people in all circles because of the high nutritional content of squid, especially the protein content of 17.9 g / 100 g of fresh squid. This study aims to determine the quality of the best squid crackers (Loligo sp.) by adding different turmeric (Curcuma domestica). The analysis carried out included: Proximate analysis (levels: water, ash, fat, protein, and carbohydrates), and Total Plate Count (TPC) tests. This study used an experimental method, completely randomized design (CRD), with 4 treatments without the administration of turmeric extract (O), dosing 12.5% (A), 15% (B) and 17.5% (C) respectively 6 repetitions. The results of the proximate test analysis, the use of turmeric extract on the making of squid crackers had a significant effect on the quality of squid crackers with the use of the best turmeric extract: 12.5% turmeric dose (moisture content: 9.622%), 17.5% turmeric dose ( fat content: 4.765%), turmeric dosage 17.5% (protein content: 18.112%), 12.5% turmeric dose (carbohydrate level: 68.253%), and 12.5% turmeric dose (ash content: 1.278%). The use of turmeric extract has a very significant effect on TPC on squid crackers, the best treatment is treatment C with a TPC value of 28,350 colonies / gram. The use of turmeric extract as an anti-bacterial ingredient in the process of making squid crackers is able to inhibit bacterial activity and has a fairly high nutrient content.

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PENGARUH INFUSA DAUN KELOR (Moringa oleifera) SEBAGAI PRESERVATIF ALAMI TERHADAP KUALITAS DAGING BABI

JURNAL KAJIAN VETERINER ◽

10.35508/jkv.v8i1.2241 ◽

2020 ◽

Vol 8 (1) ◽

pp. 1-23

Author(s):

Selviani Trivoningsi Dangur ◽

Novalino H. G. Kallau ◽

Diana Agustiani Wuri

Keyword(s):

Moringa Oleifera ◽

Ph Value ◽

Biceps Femoris ◽

Laboratory Research ◽

Plate Count ◽

Total Plate Count ◽

Randomized Design ◽

Experimental Laboratory ◽

Completely Randomized Design

Pork is one of the most consumption types of meat in the East Nusa Tenggara region.. Moringa leaf are one part of the plant which is known to have antimicrobial compounds.The aimof this research was to determine the influence of Moringa leaf infusion as a natural preservative to the quality of pork.This research is an experimental laboratory research and used a total of 48 samples of thigh pork (Biceps femoris). This research used a completely randomized design with factorial pattern. The first factor was concentration of infusion Moringa leaf consist of 0% (K0), 5% (K1), 10% (K2), and 15% (K3) and the second factor was time of storage consist of 0,6, 12, and 18 hours with 3 replications. The parameters of porkquality that have been examined: color, texture, odor, value in the Eber test, pH value, and total plate count (TPC) value.The results showed that the addition of Moringa leaf infusion change the color and odor. The Eber test shows the K3 group can last up to 18 hours. There was no significant effect of infusion concentration (P> 0.05) on the pH value and there was a very significant effect on the time of storage (P<0.01) on the pH value. There was a significant effect of infusion concentration (P<0.05) and very significant effect on the time of storage (P <0.01) on the TPC value. The value of TPC in the K3 group was below of the Standar Nasional Indonesia contamination limit for laying less than 12 hours at room temperature.

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KUALITAS PERMEN JELLY SARI BUAH PEPAYA (Carica papaya L.) DENGAN PENAMBAHAN EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.)

Jurnal Gizi dan Pangan Soedirman ◽

10.20884/1.jgps.2020.4.1.2601 ◽

2020 ◽

Vol 4 (1) ◽

pp. 26

Author(s):

Livia Adriana ◽

Franciscus Sinung Pranata ◽

Yuliana Reni Swasti

Keyword(s):

Antioxidant Activity ◽

Experimental Design ◽

Carica Papaya ◽

Plate Count ◽

Hibiscus Sabdariffa ◽

Total Anthocyanin ◽

Total Plate Count ◽

Randomized Design ◽

Completely Randomized Design

Jelly candy is a favorite snack of various ages from children to adults because it has an attractive color, delicious aroma and taste, and a chewy texture. In this study, papaya juice (Carica papaya L.) was used as a base ingredient for making jelly candy because papaya is a fruit that is very easy to find and has a relatively cheap price. In order to improve the quality of the papaya jelly candy, roselle calyx extract (Hibiscus sabdariffa L.) was also added. Roselle calyx has been known to have high antioxidants because they contain anthocyanin pigments. The purpose of this study was to determine the effect of variations in the addition of roselle calyx extract to the papaya jelly candy. The experimental design in this study was a completely randomized design with variations in the addition of roselle calyx extract by 0% (control), 15%, 30%, and 45%. The parameters tested in this study include chemical, physical, microbiological, and organoleptic qualities. The results of the research that have been carried out show the addition of 45% roselle calyx extract can produce the best papaya jelly candy based on water content, total titrated acid, antioxidant activity, total anthocyanin, texture, color, and microbiological tests which include total plate count and numbers of yeast molds. Keywords : jelly candy, papaya juice, roselle calyx extract

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Pengaruh penggunaan tepung daun sambiloto (andrographis paniculata nees) sebagai feed additive terhadap kualitas telur itik mojosari

Jurnal Sains Peternakan ◽

10.21067/jsp.v5i2.3159 ◽

2017 ◽

Vol 5 (2) ◽

pp. 92-99

Author(s):

Moh Yunus Bahtiar ◽

Dyah Lestari Yulianti ◽

Aju Tjatur Nugroho Krisnaningsih

Keyword(s):

Field Experiments ◽

Egg Yolk ◽

Feed Additive ◽

Shell Weight ◽

Egg Weight ◽

Feed Material ◽

Randomized Design ◽

Significant Difference ◽

Haugh Unit

Abstrak Penelitian ini bertujuan untuk mengetahui bagaimana kualitas telur itik Mojosari yang diberi feed additive tepung daun sambiloto pada pakan. Materi yang digunakan 100 ekor itik Mojosari, telur itik dari berbagai perlakuan dan ulangan pakan basal berupa complete feed serta tepung daun sambiloto (TDS). Metode yang digunakan adalah metode percobaan lapang menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan 4 ulangan, perlakuan yang diterapkan adalah P0 : pakan + 0% TDS, P1 : pakan + 0,2% TDS, P2 : pakan + 0,4% TDS, P3 : pakan + 0,6% TDS dan P4 : pakan + 0,8% TDS. Variabel yang diamati adalah berat telur, tebal kerabang, berat kerabang, haugh unit dan skor kuning telur. Adapun data yang didapat pada penelitian ini dianalisis sidik ragam apabila terdapat pengaruh pada perlakuan maka dilanjutkan dengan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa penggunaan TDS sebagai feed additive pada P3 memberikan perbedaan yang nyata (P<0,05) terhadap berat kerabang (9,16±0,64 g) dan pada P1 tidak berpengaruh nyata (P<0,05) pada haugh unit (106,61±3,15), tetapi pada P0, P2 dan P4 menunjukkan tidak berbeda nyata (P>0,05) terhadap berat telur (59,02±3,88 g ; 61,58±2,34 g ; 63,10±5,27 g) tebal kerabang (0,55±0,05 mm ; 0,56±0,12 mm ; 0,67±0,17 mm) serta skor kuning telur (8,50±2,38 ; 10,25±0,57 ; 10,75±1,29). Dapat disimpulkan bahwa pemberian feed additive TDS dilevel 0,6 % pada pakan itik Mojosari dapat meningkatkan berat kerabang dan pada level 0,2 % dapat meningkatkan nilai haugh unit telur itik Mojosari, akan tetapi tidak dapat meningkatkan berat telur, tebal kerabang dan skor kuning telur itik Mojosari. Abstract The purpose of this research is to find out how the quality of Mojosari duck eggs are fed feed additive of bitter leaf starch on the feed. Material used 100 ducks Mojosari, duck eggs from various treatments and replication of basal feed in the form of complete feed and bitter leaf flour (TDS). The method used was field experiments using Completely Randomized Design (RAL) with 5 treatment 4 replications, the treatment applied was P0: feed + 0% TDS, P1: feed + 0.2% TDS, P2: feed + 0.4% TDS, P3: feed + 0.6% TDS and P4: feed + 0.8% TDS. The variables observed were egg weight, thickness of the shell, weight of shell, haugh unit and egg yolk score. The data obtained in this study was analyzed variance if there is influence on the treatment then continued with the smallest real difference test (BNT). The results showed that the use of TDS as feed additive on P3 gave significant difference (P <0,05) to shell weight (9,16 ± 0,64 g) and at P1 gave significant difference (P <0.05) at Haugh units (106.61 ± 3.15), but at P0, P2 and P4 showed no significant differences (P> 0.05) with respect to egg weight (59.02 ± 3.88 g; 61.58 ± 2.34 G; 63.10 ± 5.27 g) shell thickness (0,55 ± 0,05 mm; 0,56 ± 0,12 mm; 0,67 ± 0,17 mm) and egg yolk score (8,50 ± 2.38; 10.25 ± 0.57; 10.75 ± 1.29). Based on the result of the research, it can be concluded that giving feed additive of TDS at 0,6% at feed of Mojosari duck can increase shell weight and at level 0,2% can increase haugh unit of Mojosari duck egg, but can not increase egg weight, thick of kerabang And egg yolk score of Mojosari ducks.

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Effects of contact time of natural cu-zeolite on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa

Majalah Kedokteran Gigi Indonesia ◽

10.22146/majkedgiind.40566 ◽

2020 ◽

Vol 6 (1) ◽

pp. 25

Author(s):

Erwid Fatchur Rahman ◽

Bambang Dwirahardjo ◽

Poerwati Soetji Rahajoe

Keyword(s):

Pseudomonas Aeruginosa ◽

Streptococcus Pyogenes ◽

Bacterial Growth ◽

Contact Time ◽

Plate Count ◽

Average Growth ◽

30Th Minute ◽

Total Plate Count ◽

Randomized Design ◽

Plate Count Method

Infection of a surgical wound due to bacteria is a major problem for surgical patients. Cu-zeolite is a material that can suppress bacterial growth with reversible cation characteristics and adsorption to be developed into non-toxic disinfectants for humans. Packaging uses filter paper to keep disinfectant solutions or instruments that will be sterilized clean. This study aimed to observe the effects of contact time of natural Cu-zeolite on the growth of Streptococcus pyogenes (S. pyogenes) and Pseudomonas aeruginosa (P. aeruginosa) bacteria. An experimental research was simple randomized design. Cu-zeolite 10 grams were packaged in Whatman no 42 paper bags measuring 5 x 5 cm2, contacted for 15, 30 and 45 minutes in 99.5 ml of distilled water exposed to 0.5 x 108 CFU / ml of S. pyogenes and P. aeruginosa. Subsequently, bacterial growth was calculated using total plate count method. The average growth of S.pyogenes for 15, 30 and 45 minutes (1840 ± 571.236 CFU; 29 ± 16.33 CFU and 0 CFU) while P. aeruginosa was (2776 ± 725.277 CFU; 55 ± 23.214 CFU and 0 CFU) respectively. Based on the independent t-test on Cu-zeolite, the bacterial growth in the 15th and 30th minute contact between S. pyogenes and P. aeruginosa was significantly different (pth and 30th minute contact.

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