scholarly journals Formulasi permen jelly sari buah jeruk siam

2021 ◽  
Vol 3 (2) ◽  
pp. 57-62
Author(s):  
Andi Maryam ◽  
Dian Sari
Keyword(s):  
Vitamin C ◽  
E Coli

Penelitian ini fokus pada pengolahan jeruk siam grade C atau di bawahnya (shongpi) menjadi permen jelly agar meningkatkan nilai jual buah jeruk siam baik grade C maupun “shongpi”. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan yaitu variasi konsentrasi serbuk jelly 2 gram, 6 gram, dan 10 gram, tiga kali pengulangan. Parameter yang diamati pada penelitian ini adalah kadar vitamin C, kadar sukrosa, dan cemaran mikrobia. Kadar vitamin C untuk permen jelly dengan perlakuan serbuk jelly 10 gram, 6 gram, dan 2 gram berturut-turutadalah 0,16%, 0,18%, dan 0,19%. Kadar sukrosapermen jelly jeruk dengan perlakuan serbuk jelly 10 gram, 6 gram, dan 2 gram berturut-turut adalah 47%, 44,9%, dan 49,1%. Karakteristik biologi pada permen jelly jeruk adalah angka paling mungkin untuk cemaran bakteri Escherechia coli yaitu < 3. Angka ini menunjukkan bahwa permen jelly yang dihasilkan pada penelitian ini tidak tercemar oleh E. coli.

scholarly journals Assessment of Gut Microbiota and Selected Blood Parameters in Weaned Piglets Following Supplementation with a Probiotic and Vitamin C

2020 ◽  
Vol 20 (1) ◽  
pp. 179-189
Author(s):  
Leszek Tymczyna ◽  
Beata Trawińska ◽  
Marta Kowaleczko ◽  
Anna Chmielowiec-Korzeniowska ◽  
Jerzy Lechowski
Keyword(s):  
Vitamin C ◽  
Gut Microbiota ◽  
Total Cholesterol ◽  
Hdl Cholesterol ◽  
Blood Parameters ◽  
Control Group ◽  
Weaned Piglets ◽  
E Coli ◽  
Experimental Group ◽  
Control Group Group

AbstractThe aim of the study was to assess the gut microbiota and selected haematological and biochemical blood parameters of weaned piglets following dietary supplementation with a probiotic and vitamin C. the piglets were divided into a control group (group C) and an experimental group (group E), with 30 piglets in each group. All animals received the same feed ad libitum. The animals in the control group (group C) received feed with no added probiotic and vitamin C. the piglets in the experimental group (group E) were given a supplement containing a probiotic (Bacillus cereus 1×109 CFU/kg) in the amount of 1.5 g/piglet/day and vitamin c in the amount of 300 mg/piglet/day. The supplement was administered for 28 days. The total numbers of bacteria of the family Enterobacteriaceae and of the genus Lactobacillus were determined in faeces. The erythrocyte count, haemoglobin level, haematocrit, leukocyte count, and percentages of neutrophils and lymphocytes were determined in the blood. The biochemical analysis concerned the concentration of triacylglycerols, total cholesterol, and LDL and HDL cholesterol. In group E a significant decrease (P<0.01) in the total number of Enterobacteriaceae bacteria was observed in the faeces of the piglets, accompanied by an increase in the number of lactobacilli relative to group C. E. coli was found to predominate over other microorganisms. Salmonella choleraesuis bacteria were present in the faeces of both groups before administration of the supplement, but were not found after its use. The supplement with probiotic and vitamin C caused a significant increase in the erythrocyte, haemoglobin and haematocrit levels in the blood of the piglets and a significant decrease in the concentration of triacylglycerols, total cholesterol and LDL cholesterol.

Khảo sát tiềm năng kháng khuẩn từ cao chiết lá cây ổi (Psidium guajava l.)

2020 ◽  
Vol 14 (1) ◽  
pp. 84-94
Author(s):  
Dương Nhật Linh ◽  
Nguyễn Tấn Phát ◽  
Nguyễn Doãn Thanh Liêm ◽  
Trần Thị Á Ni ◽  
Nguyễn Thanh Duy ◽  
...  
Keyword(s):  
Vitamin C ◽  
13C Nmr ◽  
Psidium Guajava ◽  
Salmonella Typhi ◽  
E Coli

Ngày nay, cùng với sự phát triển của y học, các hợp chất từ thực vật giữ vai trò chính trong việc phát hiện và phát triển các dược phẩm mới, được xem như là một trong những nguồn thay thế lý tưởng vì mức độ an toàn, không hoặc ít phản ứng phụ và có nhiều đích tác động khác nhau lên tế bào vi khuẩn nên ít có nguy cơ gây ra sự kháng thuốc, trong đó Ổi (Psidium guajava L.) rất giàu các chất chống oxy hóa, vitamin C, kali và chất xơ. Nghiên cứu dược lý cho thấy dịch chiết các bộ phận của cây ổi đều có khả năng kháng khuẩn, làm săn se niêm mạc và cầm tiêu chảy. Trong kết quả nghiên cứu này, cao ethyl axetat từ lá cây ổi có khả năng kháng khuẩn và kháng cao nhất với MRSA (31,67 ± 1,52mm). Cao chiết ethyl axetat có tác động ức chế MRSA, E. coli và Salmonella typhi ở nồng độ ức chế tối thiểu (MIC) là 1/128 tương ứng nồng độ 1,5625mg/mL và có tác động ức chế P. aeruginosa ở giá trị MIC là 1/256 tương ứng nồng độ 0,78mg/mL. Kết hợp phương pháp sắc ký cột và sắc ký lớp mỏng, chúng tôi đã phân lập và tinh chế được hợp chất PG01 có khả năng kháng MRSA cao nhất. Dựa trên dữ liệu phổ 1H, 13C-NMR cho thấy cấu trúc của hợp chất có khả năng kháng khuẩn mạnh (PG01) được xác định là 3-β-hydroxylup-20(29)-ene. Kết quả của đề tài có thể sẽ mở ra một hướng phát triển mới cho ngành Dược trước những thách thức của tình hình vi khuẩn đề kháng kháng sinh hiện nay.

scholarly journals Ascorbic Acid Changes Growth of Food-Borne Pathogens in the Early Stage of Biofilm Formation

2020 ◽  
Vol 8 (4) ◽  
pp. 553
Author(s):  
Jana Przekwas ◽  
Natalia Wiktorczyk ◽  
Anna Budzyńska ◽  
Ewa Wałecka-Zacharska ◽  
Eugenia Gospodarek-Komkowska
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Food Industry ◽  
Early Stage ◽  
Food Additives ◽  
Bacterial Biofilm ◽  
Biofilm Inhibition ◽  
E Coli ◽  
Food Borne ◽  
The Impact

Since bacterial biofilm may contribute to the secondary contamination of food during the manufacturing/processing stage there is a need for new methods allowing its effective eradication. Application of food additives such as vitamin C already used in food industry as antioxidant food industry antioxidants may be a promising solution. The aim of this research was evaluation of the impact of vitamin C (ascorbic acid), in a range of concentrations 2.50 µg mL−1–25.0 mg mL−1, on biofilms of Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes strains isolated from food. The efficacy of ascorbic acid was assessed based on the reduction of optical density (λ = 595 nm). The greatest elimination of the biofilm was achieved at the concentration of vitamin C of 25.0 mg mL−1. The effect of the vitamin C on biofilm, however, was strain dependent. The concentration of 25.0 mg mL−1 reduced 93.4%, 74.9%, and 40.5% of E. coli, L. monocytogenes, and S. aureus number, respectively. For E. coli and S. aureus lower concentrations were ineffective. In turn, for L. monocytogenes the biofilm inhibition was observed even at the concentration of 0.25 mg mL−1. The addition of vitamin C may be helpful in the elimination of bacterial biofilms. Nonetheless, some concentrations can induce growth of the pathogens, posing risk for the consumers’ health.

scholarly journals Effect of Feed Supplemented With Vitamin C on Microbial Flora of Swine Faeces and Blood Morphology

2012 ◽  
Vol 56 (2) ◽  
pp. 171-175
Author(s):  
Beata Trawińska ◽  
Jerzy Lechowski ◽  
Antoni Polonis ◽  
Marta Kowaleczko
Keyword(s):  
Vitamin C ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Intestinal Flora ◽  
Bacterial Count ◽  
Control Group ◽  
Total Bacterial Count ◽  
E Coli ◽  
Bacteriological Evaluation ◽  
Experimental Group

Abstract The studies involved gilts 6 weeks pre-partum allocated into the control and experimental groups. The experimental group received vitamin C at the dose of 2.5g/100 kg b.w./day. Faeces for analyses were collected for 3- and 6- week period of vitamin C dietary inclusion. The faecal material underwent quantitative and qualitative bacteriological evaluation. The count of Enterobacteriaceae was evaluated. The qualitative examination of these bacteria was made using the micro-plate method API 20E. Feed supplemented with vitamin C contributed to a statistically significant decline of the total bacterial count of Enterobacteriaceae in faeces. The qualitative bacterial analyses showed the presence of E. coli, Providecia sp., Proteus sp., and Salmonella sp. in the control and experimental groups. Enterobacter sp. was recovered only in the control group, while Lactobacillus sp. in the experimental group. The dietary administration of vitamin C significantly decreased the number of the studied bacterial species, except Salmonella rods. No statistically significant differences in the number of most blood morphotic elements following the 6- week supply of vitamin C were found; only the granulocyte count significantly increased, while lymphocyte numbers declined. Ascorbic acid inhibited the growth of pathogenic intestinal flora and reduced the pathogenic and relatively pathogenic bacteria count in the gastrointestinal tract and notably contributed to enhanced growth of beneficial bacteria.

scholarly journals Studi Aspek Mikrobiologis Dan Kimiawi Minuman Jamu Gendong Kunyit Asam Di Jimbaran dan Kedonganan, Bali

2021 ◽  
Vol 10 (1) ◽  
pp. 97
Author(s):  
Thanastasya Yolanda ◽  
Luh Putu Trisna Darmayanti ◽  
Agus Selamet Duniaji
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Vitamin C ◽  
Survey Method ◽  
Total Acid ◽  
Total Vitamin ◽  
E Coli ◽  
Two Samples ◽  
Microbiological Aspect ◽  
Medicinal Properties

“Jamu Gendong Kunyit Asam (Tamarind Turmeric Sour Herbs)” is a typical Indonesian drink that is quite famous which is one of the legacies of the Indonesian nation’s ancestors who have medicinal properties in maintaining health and for healing a disease. This study aims to determine aspects of chemistry and microbiology as well as food safety of Tamarind Turmeric Sour Herbs in Jimbaran and Kedonganan, Bali by using survey method and research. The observed variabels include total microbial, coliforms, Escherichia coli, pH, total acid, total vitamin C. The results of microbiological aspect test in this study showed that two samples of Jamu Gendong Kunyit Asam (Tamarind Turmeric Sour Herbs) at Jimbaran and Kedonganan,Bali does not meet the requirements according to SNI 7388:2009 namely samples B and E with indigo in sample B that is 30 x 10-1 and sample E which is 1 x 10-1. The result of chemistry aspect are if the increase in total vitamin C will effect the increase in total acid and pH will be more acid. Besaide that, E. coli bacteria can grow at a pH of 5-7 and the compound content of the tamarind fruit is also antibacterial so that most of Jamu Gendong Kunyit Asam (Tamarind Turmeric Sour Herbs) at Jimbaran and Kedonganan are not contaminated with E.coli.

scholarly journals EFFECT OF BOILING AND MICROWAVE ASSISTED PROCESSING ON THE ANTIMICROBIAL EFFICACY OF VITAMIN–C IN EMBLICA OFFICINALIS

2020 ◽  
pp. 102-105
Author(s):  
PAYEL SUTRADHAR ◽  
SHALINI GHOSH ◽  
BINITA KUMARI ROY
Keyword(s):  
Vitamin C ◽  
Crude Extract ◽  
Active Form ◽  
Citrus Fruit ◽  
Inhibitory Effect ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Emblica Officinalis ◽  
E Coli ◽  
Microwave Assisted

Objective: The present work aimed to expand the awareness of restoring vitamin-C in its active form on different heat exposures. The effect of microwave-assisted processing and boiling of the aqueous crude extract of citrus fruit Emblica officinalis (amla) has been correlated with its antimicrobial efficacy against E. coli. Methods: The aqueous crude extract of dried amla pulp exposed to microwave radiation(600W,5 min) and boiling (5 min) were titrimetrically estimated for vitamin-C content by DCPIP-(2,6, Dinitrophenol indophenol) method and compared the same with the untreated sample. These three samples were studied for their effect on the growth pattern of E. coli turbidimetrically. The antimicrobial susceptibility test by agar cup well diffusion method was further followed to measure the zone of inhibitions (ZOI) for these three test extracts against E. coli. Results: The total estimated vitamin-C content was 26.76 mg/100g, 25.35 mg/100g and 21.12 mg/100g in the untreated extract (UTE), microwaved extract (MWE) and boiled extract (BE) respectively. At a higher concentration (0.8 mg/ml), the UTE showed a greater ZOI of 20 mm and a comparable ZOI of 18 mm for the MWE against E. coli. In addition, a reduced ZOI of 10 mm was recorded in case of the BE. At a lowest concentration (0.05 mg/ml), the UTE inhibited the growth with a least ZOI of 7 mm, whereas no inhibition zones were detected for MWE and BE at this concentration. Conclusion: The present investigation demonstrated the effect of boiling and microwave-assisted processing on the content of bioactive vitamin-C and its antimicrobial activity. The DCPIP method calculated a more vitamin-C retention in the MWE than the BE. As the boiling method destroyed the vitamin more rapidly, a higher growth rate of E. coli was measured in the presence of BE than the UTE and MWE. In addition, the antimicrobial assay also showed a least inhibitory effect against E. coli in the presence of the BE. A moderate inhibitory effect for MWE was also detected. Thus the present investigation proved that the boiling process destroys vitamin-C present in a food sample to a higher extent than the microwave-assisted processing.

scholarly journals Strategies to control bacteriophage infection in a threonine bioprocess

2009 ◽  
Author(s):  
◽  
Nolwazi Cele
Keyword(s):  
Vitamin C ◽  
Mitomycin C ◽  
Uv Light ◽  
Cost Effective ◽  
Coli Strain ◽  
Host Bacterium ◽  
Bacterial Strains ◽  
E Coli ◽  
Bacterial Cultures ◽  
Bacteriophage Infection

Production of numerous biotechnologically-important products such as threonine is based on cultivation of bacterial cultures. Infection of these bacterial cultures by bacteriophages has a detrimental effect in the production of these bioproducts. Despite this, most people controlling these bioprocesses do not recognize the early signs of bacteriophage infection. SA Bioproducts (Ply) Ltd was no exception and has suffered tremendous loss of production time after bacteriophages infected threonine producing E. coli strain B. This study was aimed at developing assays to control and prevent bacteriophage infection at this company. These included determining the source of phages by monitoring the process plant environment, optimising the detection and enumeration methods so as to monitor the levels of bacteriophages in the environment, identification of bacteriophages in order to determine the number of bacteriophages capable of infection threonine producing E. coli strain B, treatment and of phages, and possible prevention of phage infection. Adam's DAL method was very efficient at detecting phages in the samples collected at various areas (sumps, odour scrubber, process water, and soil) around the plant for 16 weeks. High levels of phages were found in the sumps and this was identified as the source of infection. Samples collected were grouped together according to their source. The samples were enriched and purified in order to characterise them. The prevalent phage in all samples was identified as a T1-like phage. Bacterial strains that grew on the plate in the presence of phages were assumed to be resistant to phages or contained lysogenic phages which would explain the new lytic cycles that were observed whenever these resistant strains were used for production. UV light, green v indicator plates, and a mutagen (Mitomycin C) were used to detect Iysogens. Mitomycin C at 1 IJg/ml was found to be most effective in detecting lysogenic phages. This was shown by new plaque forming units that were visible on the DAL plates. Temperature (heat), chemicals, and inhibitors (vitamins) were investigated as strategies for prevention and treatment of bacteriophage infection. Bacteriophage samples were exposed to 70, 80, 100, and 120°C. At these temperatures pfu counts in the samples were reduced significantly. At 120°C there was a complete inactivation of bacteriophages within 30 minutes. Chemicals investigated such as sodium hydroxide and Albrom 100T were capable of complete deactivation of bacteriophages at a very low concentration (0.1%). Therefore, these chemicals can be used to clean the plant area and sumps. Vitamins C, K and E solutions were investigated to determine their inhibitory effect on bacteriophages. Vitamin C, K and E reduced pfu counts by 3, 2, and 4 logs, respectively. Therefore vitamin C and E solutions were mixed and to determine if mixing them would enhance their inactivation capabilities. This resulted in a reduction greater than 9 logs of phage in the sample (from 7.7 x 109 to 3 pfu/ml). The host bacterium was also exposed to this mixture to determine effect of the vitamin mixture on its growth. It was found that there was no effect exerted by this mixture on the host bacteria. This proved to be an ideal mixture for combating phages during fermentation. However, vitamin E is not cost effective for co-feeding in 200 m' fermenters, and therefore vitamin C solution was a cost-effective alternative. It was concluded that bacteriophage contaminated bioprocessing plant should be properly cleaned using a combination of heat and chemicals. Bacteriophage infection should be prevented by employing inhibitors.

scholarly journals OPTIMASI EKSTRAKSI SENYAWA FENOLIK DARI KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa) BERBANTU GELOMBANG MIKRO

Agrin ◽  
2018 ◽  
Vol 21 (2) ◽  
Author(s):  
Ali Maksum ◽  
Ike Sitoresmi Mulyo Purbowati
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Output Power ◽  
Conventional Method ◽  
Extraction Time ◽  
Total Phenolic ◽  
Total Phenols ◽  
Inhibitory Zone ◽  
E Coli ◽  
Ic50 Value

Kelopak bunga rosella adalah sumber senyawa fenolik yang baik. Dalam penelitian ini ekstraksi dibantugelombang mikro digunakan untuk mengekstrak senyawa fenolik dari kelopak bunga Rosella. Denganmenggunakan metode respon permukaan, efek daya gelombang mikro, konsentrasi etanol, dan waktu ekstraksipada hasil total fenol yang diselidiki dan kondisi optimal ditentukan sebagai berikut: daya gelombang mikro 250W, konsentrasi etanol 78,36% dan waktu ekstraksi 4,91 menit. Nilai perkiraan untuk total hasil fenol, 24,61 mg/gdiperoleh pada kondisi tersebut. Percobaan verifikasi pada kondisi optimum, terdiri dari 4 putaran, dilakukan danhasil praktis 23,77 + 0,25 mg/g jumlah fenol lebih tinggi dari pada metode konvensional 19,84 + 0,46 mg/g.Dibandingkan dengan metode konvensional, antosianin, vitamin C dan hasil ekstraksi dibantu gelombang mikroadalah 14,80 + 0,08, 10,74 + 0,14 mg/g dan 22,09 + 3,3% yang lebih tinggi dan berbeda secara signifikan dalamtingkat kepercayaan 95% dari pada ekstraksi konvensional, yaitu 9,25 + 0,04, 9,99 + 0,16 mg/g dan 16,18 + 1,9%.Aktivitas antibakteri yang dinyatakan dengan nilai zona hambat adalah 12,6 + 0,6 mm pada E. coli dan 11,6 + 0,3mm terhadap S. aureus. Nilai IC50 ekstrak adalah 202,47 μL/mL. Dibandingkan dengan metode konvensional,zona hambatan 10,2 + 0,4 mm pada E. coli dan 9,8 + 0,6 mm pada S. aureus. Aktivitas antioksidan dinyatakandengan nilai IC50293,09 μL/mLKata kunci: kelopak bunga Rosella, ekstraksi berbantu gelombang mikro, fenolABSTRACTRoselle calyx is an excellent source of phenolic compounds. In this study, microwave-assisted extractionwas applied to extract the phenolic compounds from Roselle calyx. By using response surface methodology, theeffects of microwave output power, ethanol concentration, and extraction time on total phenolic yield wereinvestigated and the optimal conditions were determined as follows: microwave output power 250 W, ethanolconcentration 78.36% and extraction time 4.91 min. The estimated values for total phenols yield, 24.61 mg/g wasobtained at those conditions. A verification experiment at the optimum condition, consisting of 4 runs, wasperformed and the practical yield 23.77 + 0.25 mg/g total phenols higher than using conventional method 19.84+ 0.46 mg/g was obtained. Compared with conventional method, anthocyanin, vitamin C and yield of microwaveassisted extraction were 14.80 + 0.08, 10.74 + 0,14 mg/g and 22.09 + 3.3 % which were higher and significantlydifferent within 95% confidence level than conventional extraction , which were 9.28 + 0.04, 9.99 + 0.16 mg/gand 16,18 + 1,9%, respectively. Antibacterial activity expressed with inhibitory zone value was 12.6 + 0.6 mmagainst E coli and 11.6 + 0.3 mm against S aureus. IC50 value of the extract was 202.47 μL/mL.Compared withconventional method, with inhibitory zone of 10. 2 + 0.4 mm against E coli and 9.8 + 0.6 mm against S aureus.Antioxidant activity expressed with IC50 value of 293.09 μL/mL.Key words: roselle calyx, extraction, microwave, phenols

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