Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn. in Rats

2017 ◽  
Vol 10 (1) ◽  
pp. 3616-3622
Author(s):  
Kruti M Patel ◽  
Samir K Shah
Keyword(s):  
Uric Acid ◽  
Calcium Phosphate ◽  
Ethylene Glycol ◽  
Methanolic Extract ◽  
Alcoholic Extract ◽  
Lawsonia Inermis ◽  
Urea Nitrogen ◽  
Standard Drug ◽  
Kidney Homogenate ◽  
Model Control

Lawsonia inermis Linn., commonly called as Henna belongs to family Lythraceae. Traditionally, it has been reported to have therapeutic activity in bronchitis, diabetes, antimicrobial, antibacterial, trypsin inhibitory, cytotoxicity, wound healing, antioxidant, anti-inflammatory, analgesic. However, the antiurolithiatic activity of the bark extract of  L. inermis Linn. is not known. In this study, we investigated protective effect of the alcoholic extract of L. inermis bark against ethylene glycol induced urolithiasis and its possible underlying mechanisms using male wistar albino rats. Animals were divided into seven groups and urolithiasis was induced by ethylene glycol (0.75% v/v) in drinking water for 28 days. Methanolic extract of Lawsonia inermis (MELI) bark (300 & 500 mg/kg, p.o.) were administered once daily from 15th day to 28th day as curative regimen and from 1st day to 28th day as preventive regimen. Cystone (750 mg/kg, p.o.) was used as a standard drug. After 28 days, various biochemical parameters like urine volume, pH were measured. Calcium, phosphate and oxalate were measured in urine and kidney homogenate. Serum creatinine, uric acid and urea nitrogen were estimated. Histopathology of kidney also studied. Treatment with the MELI extract significantly restored all elevated parameters including calcium, phosphate and oxalate in urine and kidney homogenate; creatinine, uric acid and urea nitrogen in serum when compared to model control group. The histopathological study of the kidney also supported the above results. It can be concluded that methanolic extract of bark of Lawsonia inermis Linn. has significant antiurolithiatic effect in experimental rats.

Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn. in Rats

2017 ◽  
Vol 10 (3) ◽  
pp. 3728-3735
Author(s):  
Kruti M Patel ◽  
Samir K Shah
Keyword(s):  
Uric Acid ◽  
Calcium Phosphate ◽  
Urine Volume ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Urea Nitrogen ◽  
Standard Drug ◽  
Kidney Homogenate ◽  
Group I ◽  
Model Control

This study was conducted to evaluate the potential antiurolithiatic effects of Lawsonia Inermis Linn. in rat models. Animals were divided into seven groups and urolithiasis was induced by ethylene glycol (0.75% v/v) in drinking water to all groups (Groups II-VII) except normal control (Group I) for 28 days. Methanolic extracts of Lawsonia inermis (MELI) bark (300 & 500 mg/kg, p.o.) were administered once daily, from 15th day to 28th day as curative regimen and from 1st day to 28th day as preventive regimen. Cystone (750 mg/kg, p.o.) was used as a standard drug for comparison. After 28 days, various biochemical parameters like urine volume, pH were measured. Calcium, phosphate and oxalate were measured in urine and kidney homogenate. Serum creatinine, uric acid and urea nitrogen were estimated. Histopathology of kidney was investigated. Treatment with the MELI extract significantly (p<0.05) restored all elevated parameters including calcium, phosphate and oxalate in urine and kidney homogenate; creatinine, uric acid and urea nitrogen in serum when compared to model control group. The histopathological study of the kidney also supported the above results. It was concluded that methanolic extract of bark of Lawsonia inermis Linn. has significant antiurolithiatic effect in experimental rats.   

EVALUATION OF ANTINEPHROLITHIATIC ACTIVITY OF MALLOTUS PHILIPPINENSIS LINN. IN RATS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 77-82
Author(s):  
S. K. Shah ◽  
◽  
K. R Patel ◽  
T. V. Patel ◽  
T. B. Patel
Keyword(s):  
Uric Acid ◽  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Rat Model ◽  
Serum Levels ◽  
Kidney Damage ◽  
Alcoholic Extract ◽  
Protective Group ◽  
Kidney Homogenate ◽  
Model Control

The present study was aimed to evaluate antinephrolithiatic activity of alcoholic extract of Mallotus philippinensis Linn. on ethylene glycol (0.75% V/V) induced nephrolithiasis in rat model. The leaves of Mallotus philippinensis Linn. were extracted and antinephrolithiatic activity was evaluated at the dose of 250 & 500 mg/kg b.w., p.o. Treatment with alcoholic leaves extract of Mallotus philippinensis decreased levels of calcium oxalate, phosphate in urine and kidney homogenate. Serum levels of creatinine, urea and uric acid were also decreased significantly in curative as well as protective group as compared to model control rats. The alcoholic extract of Mallotus philippinensis Linn. at the dose of 500 mg/kg showed no microcrystalline deposition and kidney damage which enables us to confirm the protective and curative potential of Mallotus philippinensis Linn. on ethylene glycol induced nephrolithiasis in rats. It can be concluded that alcoholic leaves extract of Mallotus philippinensis possess a significant antinephrolithiatic activity.

scholarly journals Rotula Aquatica Lour Aqueous Extract as Anti-Urolithiatic Agent in Experimentally Induced Urolithiatic Rat Model

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Bhavaniamma Vijayakumari ◽  
Venkatachalam Sasikala ◽  
Singanallur Ramu Radha ◽  
Hiranmai Yadav Rameshwar
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Blood Urea Nitrogen ◽  
Serum Protein ◽  
Root Extract ◽  
Urea Nitrogen ◽  
Aqueous Root Extract ◽  
Phosphate Magnesium

Rotula aquatica Lour is traditionally used to regulate the kidney and bladder stones and root decoction is used for diuretic activity. The present study was carried out to assess the antiurolithiatic activity of the aqueous root extract of the plant in urolithiatic rats.The extract was administered to the calcium oxalate (CaOx) urolithiatic rats orally at 100 and 200 mg/kg body weight along with ethylene glycol (EG) for 28 days. On 28 day, 24 h urine was collected from individual rats and urine was analysed for protein, calcium, phosphate, magnesium, uric acid, oxalate and creatinine. On same day the serum protein, calcium, phosphate, magnesium, uric acid, oxalate, creatinine and blood urea nitrogen levels were also estimated in each animal. The kidney homogenate was used for the estimation of calcium, oxalate and phosphate. The paraffin kidney sections were prepared to observe the CaOx deposits. The paraffin kidney sections showed significant histopathological changes. The treatment with aqueous root extract of Rotula aquatica at 100 and 200 mg/kg body weight doses significantly decreased the urine protein, calcium, phosphate, uric acid, creatinine and oxalate, serum protein, calcium, phosphate, uric acid, creatinine, blood urea nitrogen and oxalate and renal tissues calcium, phosphate and oxalate, in EG induced urolithiasis after 28 days.The present study indicated aqueous root extract of Rotula aquatica at the dose of 200 mg/kg body weight as more effective in decreasing the urolithiasis and regeneration of renal tissues in male rats. The extract has a potential to cure the urolithiasis.

scholarly journals Targeting the antiurolithiatic activity by entrapping the bioactive compounds of Asparagus racemosus in chitosan nanoparticles on ethylene glycol induced renal calculi in Wister albino rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5867-5875
Author(s):  
Kishore Bandarapalle ◽  
Prasanna Raju Yalavarthi ◽  
Chandra Sekhar Kothapalli Bannoth
Keyword(s):  
Uric Acid ◽  
Ethylene Glycol ◽  
Creatinine Clearance ◽  
Bioactive Compounds ◽  
Chitosan Nanoparticles ◽  
Renal Calculi ◽  
Asparagus Racemosus ◽  
Standard Drug ◽  
Antioxidant Parameters

The objective of our research is to investigate the antiurolithiatic intervention of bioactive compounds of Asparagus racemosus loaded Chitosan nanoparticles (BACARNPs) on ethylene glycol engendered renal calculi in male Wister rats. The efficiency of bioactive compounds of A. racemosus (BACAR) at 800 mg/kg p.o and BACARNPs at 800 mg equivalent weight of BACAR/kg p.o was validated in ethylene glycol 0.75% (v/v) and ammonium chloride 1% (w/v) mediated renal calculi in rats. Cystone (750 mg/kg, p.o.) has been used as a standard drug. Urinary variables comprise calcium, magnesium, oxalate, phosphate, uric acid, creatinine, urine pH, urine volume, and Creatinine clearance; Serum parameters include creatinine, blood urea nitrogen (BUN), calcium and uric acid; calcium and oxalate deposition in the kidney were assessed. In vivo antioxidant parameters include lipid peroxidation, superoxide dismutase, catalase, and glutathione were determined and histopathological studies were also examined. In both control groups, a substantial increase in urinary excretion of calcium, oxalate, and their intensification in the kidney; enhanced amounts of phosphate, uric acid, and reduced magnesium levels in urine; elevated serum creatinine, BUN, calcium and uric acid; Creatinine clearance was declined were observed and normalized in treated groups. In vivo antioxidant parameters and histopathological variations reinstated to conventional form. Chitosan serves as a ligand to renal epithelial cells leads to improved agglomeration of BACAR in kidney compared to BACAR administered solitarily results in increased antiurolithiatic activity.

scholarly journals Anti - inflammatory activity of ten indigenous plants in carrageenan induced paw oedema in albino rats

Biomedicine ◽  
2021 ◽  
Vol 41 (3) ◽  
pp. 649-653
Author(s):  
Manjula Shimoga Durgoji Rao ◽  
Sucheta Prasanna Kumar ◽  
Vasanthalaxmi Krishnanada Rao
Keyword(s):  
Tectona Grandis ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Alcoholic Extract ◽  
Lawsonia Inermis ◽  
Indigenous Plants ◽  
Standard Drug ◽  
Crude Extracts ◽  
Phytochemical Compounds ◽  
Anti Inflammatory

Introduction and Aim: Inflammation is a symptom associated with many diseases, can be treated with steroidal and non-steroidal anti-inflammatory drugs, which can cause severe side effects when used as long-term treatments. Plants have the ability to synthesize a wide verity of phytochemical compounds as secondary metabolites which shows anti-inflammatory activity. The anti-inflammatory activity of crude extracts of leaves of Jasminum grandiflorum (Jg), Vinca rosea (Vr), Azadirachta indica (Ai), Lawsonia inermis (Li), Nerium indicum (Ni), Calotropis gigantea (Cg), Tectona grandis (Tg), Andrographis paniculata (Ap), Tabernaemontana corymbosa (Tc) and Marsedinia volubilis (Mv) as well as alcoholic extracts of leaves of Cg, Tg and Ap were evaluated in Wistar rats.   Methods: Acute anti-inflammatory activity of crude extracts of ten indigenous plants were studied by calculating the volume changes in the hind paw after injecting carrageenan in rats comparing with Ibuprofen which was used as a standard drug in this study. Anti-inflammatory effect of alcoholic extract of leaves of Ap, Tg and Cg (200mg/kg body weight) also studied.   Results: The groups treated with crude extract & alcoholic extract of Cg, Tg and Ap showed significant reduction in the oedema compared to control and other plant extracts. The result was very significant in Ap treated rats.   Conclusion:  Plants are one of the most important sources of medicines. In our study, the anti-inflammatory activity of ten indigenous plants could be due to presence of naturally occurring antioxidant flavonoids. The leaves of Ap are very rich in flavonoids, could be the reason for significant anti-inflammatory activity found in Ap treated rats.

scholarly journals Short-Term Tolvaptan Increases Water Intake and Effectively Decreases Urinary Calcium Oxalate, Calcium Phosphate and Uric Acid Supersaturations

2016 ◽  
Vol 195 (5) ◽  
pp. 1476-1481 ◽  
Author(s):  
Wisit Cheungpasitporn ◽  
Stephen B. Erickson ◽  
Andrew D. Rule ◽  
Felicity Enders ◽  
John C. Lieske
Keyword(s):  
Uric Acid ◽  
Calcium Phosphate ◽  
Calcium Oxalate ◽  
Water Intake ◽  
Urinary Calcium ◽  
Short Term

Protective effect of the hydro-alcoholic extract of Rubia cordifolia roots against ethylene glycol induced urolithiasis in rats

2010 ◽  
Vol 48 (4) ◽  
pp. 1013-1018 ◽  
Author(s):  
Kalyani Divakar ◽  
A.T. Pawar ◽  
S.B. Chandrasekhar ◽  
S.B. Dighe ◽  
Goli Divakar
Keyword(s):  
Ethylene Glycol ◽  
Protective Effect ◽  
Alcoholic Extract ◽  
Rubia Cordifolia

SCREENING OF ANTI-ANXIETY ACTIVITY OF UNRIPE FRUITS OF MUSA PARADISIACA LINN.

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (09) ◽  
pp. 33-35
Author(s):  
C. S Hallikeri ◽  
◽  
S. D Joshi ◽  
P Patil ◽  
V. H Kulkarni ◽  
...  
Keyword(s):  
Amino Acids ◽  
Operant Behavior ◽  
Albino Rats ◽  
Alcoholic Extract ◽  
Standard Drug ◽  
Musa Paradisiaca ◽  
Cns Depressant ◽  
Phytochemical Investigation ◽  
Cns Depressant Activity ◽  
Unripe Fruits

The alcoholic extract of unripe fruits of Musa paradisiaca Linn. (Musaceae) was evaluated for antianxiety activity by using operant behavior (behavioral disinhibition) model of anxiety in albino rats. Phytochemical investigation of alcoholic extract revealed the presence of flavonoids, amino acids,vitamins and carbohydrates. Alcoholic extract showed significant anti-punishment and anti-frustration activities at dose level of 500 mg/kg/day for 5 days which is comparable to standard drug, diazepam 5mg/kg. The presence of amino acid and flavonoids in alcoholic extract could be attributed for the antianxiety and other CNS depressant activity.

scholarly journals ARISTOLOCHIA BRACTEOLATA – ANTIHYPERGLYCEMIC AND ANTIHYPERLIPIDEMIC ACTIVITY IN DEXAMETHASONE-INDUCED DIABETIC RAT MODEL

2017 ◽  
Vol 10 (8) ◽  
pp. 75
Author(s):  
Ganga Rajum ◽  
Hema Sundar Reddy T ◽  
Hema Sundar Reddy T
Keyword(s):  
Blood Glucose ◽  
Methanolic Extract ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Antihyperlipidemic Activity ◽  
Aristolochia Bracteolata

  Objective: The present study was aimed to evaluate antihyperglycemic and antihyperlipidemic activities of methanolic extract of Aristolochia bracteolata (MEAB) against dexamethasone-induced diabetic rat model.Methods: Methanolic extract was prepared by soxhlet extraction and was evaluated for antihyperglycemic and antihyperlipidemic activity using dexamethasone-induced model. The MEAB was administered orally at a dose of 200 and 400 mg/kg body weight glibenclamide was used as standard drug. On 0th and 11th day, blood was collected by retro-orbit plexus.Results: In this model blood glucose levels were determined on 0th and 11th days and MEAB significantly reduced the blood glucose levels in diabetic rats. The effect of MEAB on serum lipid profile such as total cholesterol (TC), triglycerides (TGs), low-density lipoprotein (LDL), very LDL (VLDL), and high-density lipoprotein (HDL) was also measured on the 11th day in the diabetic rats. Significant reduction in TC, TGs, LDL, and VLDL levels and improvement in HDL level were observed in diabetic rats.Conclusion: From the results, it was found that the MEAB possess antihyperglycemic and antihyperlipidemic activities.

scholarly journals Nephroprotective Property of C. chayamansa Aqueous Leaf Extract in Diabetic Rats

2020 ◽  
pp. 72-84
Author(s):  
Jonathan Dingkwoet Dabak ◽  
Rose Titus Kuyambana ◽  
Titilayo Omolara Johnson ◽  
Jonathan Latrwang Dabal
Keyword(s):  
Uric Acid ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Histopathological Study ◽  
Standard Drug ◽  
Aqueous Leaf Extract ◽  
Group 5 ◽  
Group 2 ◽  
High Doses ◽  
Test Control

Aim: To evaluate the nephroprotective property of Cnidoscolus chayamansa aqueous leaf extract in diabetic rats. Study Design: Rats were randomly divided into five groups with group 1 as the normal control. Diabetic was induced in groups 2-4. Group 2 was used as the test control while groups 3 and 4 were treated with different concentrations of the leaf extract; group 5 was treated with the standard drug, glipizide. Place and Duration of Study: Departments of Biochemistry and Anatomy, University of Jos, Nigeria, between August to November, 2019. Methodology: Fourty (40) male albino Wistar rats were grouped into five groups. The rats were treated for fourteen days and then sacrificed by decapitation after anaesthesia. Blood was collected for biochemical parameters; kidney was excised and stored in formaldehyde until required for histopathological study. Serum urea, creatinine, uric acid, sodium, potassium, chloride and bicarbonate were determined using appropriate methods. Results: The test control had a significant (P < .05) decrease in the concentrations of Na+, Cl- and HCO3- ions; significant (P < .05) increases in serum K+ ion, urea, uric acid and creatinine. Treatments of the test groups with the different doses of the leaf extract and the standard drug increased the concentration of Na+ ion which was not significantly (P < .05) different from the test control. On the other hand, the concentration of Cl- and HCO- ions  were significantly (P < .05) increased; the concentrations of K+, urea, uric acid and creatinine were significantly (P < .05) decreased. The histochemistry of the kidneys revealed that the injury brought about under diabetic condition was ameliorated with the treatments with the low and high doses of the leaf extract, and the standard drug. Conclusion: The results show that the aqueous leaf extract has nephroprotective property.

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