Determining Visfatin / NAMPT Serum Levels by ELISA Technique, in Pregant Women with Preeclampsia

2017 ◽  
Vol 68 (9) ◽  
pp. 2108-2111 ◽  
Author(s):  
Larisa Diana Mocan Hognogi ◽  
Mocan Radu Florin Hognogi ◽  
Andrei Malutan ◽  
Anca Daniela Farcas ◽  
Luminita Vida Simiti
Keyword(s):  
Birth Weight ◽  
Inflammatory Response ◽  
Neonatal Mortality ◽  
Plasma Levels ◽  
Serum Levels ◽  
Severe Form ◽  
Control Group ◽  
Elisa Technique

Preeclampsia is a syndrome that affects approximately 2-8% of all pregnancies [1] and is closely linked to a particularly elevated inflammatory response, its presence resulting in high maternal and neonatal mortality. Several adipokins have been found to be involved in this process. Visfatin is a cytokine that exerts its effects especially on metabolism and immunity. In this study we determined the serum profile of Visfatin using the ELISA technique, to the 40 patients diagnosed with preeclampsia and we compared it to 16 pregnant patients without complications. Patients with preeclampsia had lower, but statistically not significant plasma levels compared to those in the control group, 11.04 � 7.14 (3.57-17.97) vs 8.88 � 7.91 (1.37-17.85). The lowest Visfatin levels have been recorded in the most severe form of preeclampsia. Visfatin does not significantly influence the birth weight of the foetuses (p = 0.08).

scholarly journals Effects of budesonide and probiotics enemas on the systemic inflammatory response of rats with experimental colitis

2007 ◽  
Vol 22 (suppl 1) ◽  
pp. 40-45 ◽  
Author(s):  
Mardem Machado de Souza ◽  
José Eduardo de Aguilar-Nascimento ◽  
Diana Borges Dock-Nascimento
Keyword(s):  
Inflammatory Response ◽  
Experimental Colitis ◽  
Serum Levels ◽  
Systemic Inflammatory Response ◽  
Control Group ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Inflammatory Status ◽  
Male Wistar Rats ◽  
Group 5

PURPOSE: The aim of this study was to investigate the effect of enemas containing probiotics and budesonide on the systemic inflammatory response in experimental colitis. METHODS: Fifty male Wistar rats with experimental colitis induced by 10% acetic acid enema were randomized to five groups (10 rats each) according to the treatment: group 1 - saline solution, group 2 - budesonide (0.75 mg/kg/day), group 3 - probiotics (1mg/day), group 4 - probiotics plus budesonide, and group 5 - control, with not-treated rats. The following variables were studied: body weight, serum levels of albumin, C-reactive protein and interleucine-6 (IL-6). RESULTS: All animals lost weight between the beginning and the end of the experiment (280+ 16 mg versus 249+21 mg, p< 0.001). There was a significant decrease in the serum albumin between the normal pre-induction level (3.45 + 0.49mg/dL) and the 1st day after colitis induction (1.61+051mg/dL, p< 0.001) in all treated groups when compared to the control group. C- reactive protein increased after induction and diminished on the 7th day in all groups. In the control group there was an increase in the IL-6 after colitis induction. None of the treated groups significantly differed from IL-6 pre-colitis status (p>0.05). Only probiotic rats presented a significant decrease of IL-6 than controls (0,30±0,08 mg/dL vs. 0,19±0,03 mg/dL; p<0.01). CONCLUSION: Probiotic associated with budesonida Probiotics are effective to diminished inflammatory status mediated by IL-6 in experimental colitis.

scholarly journals Angiotensin (1-7) Attenuates Sepsis-Induced Acute Kidney Injury by Regulating the NF-κB Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Ying Zhu ◽  
Daliang Xu ◽  
Fang Deng ◽  
Yonglin Yan ◽  
Jian Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Kidney Injury ◽  
Inflammatory Response ◽  
Cystatin C ◽  
Kidney Injury ◽  
Serum Levels ◽  
Control Group ◽  
Protective Mechanism ◽  
Ang Ii ◽  
Urea Nitrogen

This study explores the protective mechanism of angiotensin (1-7) [Ang-(1-7)] on kidneys by examining its effects on renal histomorphology, inflammatory response, oxidative stress, and NF-κB signaling in mice suffering from sepsis-induced acute kidney injury. A sepsis-induced acute kidney injury mouse model was established by intracervically injecting lipopolysaccharides (LPS group), followed by the administration of Ang-(1-7) [LPS + Ang-(1-7) group]. The serum levels of urea nitrogen, creatinine and cystatin. c were measured with an automatic biochemical analyzer, and changes in proinflammatory cytokines and angiotensin II (Ang II) in the serum and kidneys were quantified by enzyme-linked immunosorbent assays. Changes in oxidative stress indices in the renal cortex were detected by colorimetry. The localization of Ang II in kidneys was examined by immunohistochemistry. Western blotting was used to examine phosphorylated NF-κB-p65 and IκBα levels in kidneys. Compared with the control group, the serum levels of urea nitrogen, creatinine and cystatin. c were increased, whereas the levels of Ang II, TNFα, IL-1β, IL-6, and malondialdehyde (mda) were increased significantly. The levels of Ang II and phosphorylated NF-κB-p65 were elevated in kidneys, whereas the levels of superoxide dismutase (sod), Total antioxidative capacity (TAOC), and inhibitor of NF-κB (IκBα) were reduced in the LPS group (p &lt; 0.05). Pathological damage was also observed in kidneys of LPS-group mice. In Pearson correlation analysis, there was a positive correlation between Ang II and phosphorylated NF-κB-p65 levels, and a negative correlation between Ang II and IκBα levels (p &lt; 0.05). After the application of Ang-(1-7), the levels of urea nitrogen, creatinine, cystatin. c, Ang II, TNFα, IL-1β, IL-6, and mda, as well as the expression of Ang II and phosphorylated NF-κB-p65 in kidneys of LPS + Ang-(1-7)-group mice, were lower than those in kidneys of LPS-group mice, but the levels of sod, TAOC, and IκBα were higher than those of LPS-group mice (p &lt; 0.05). Pathological changes were less severe in mice of the LPS + Ang-(1-7) group. Overall, Ang-(1-7) can decrease the Ang II level, inhibit NF-κB signaling, reduce the inflammatory response, decrease oxidative stress, and mitigate sepsis-associated acute kidney injury.

scholarly journals Comparative advantage of anti-Mullerian hormone over other ovarian reserve metric (basal hormonal test) in prediction of fertility in women with varying menstrual cycle

2022 ◽  
Vol 9 (1) ◽  
pp. 749-758
Author(s):  
Fidelis Ohiremen Oyakhire ◽  
Cedric Odaro ◽  
Enehizena Osaro Ogie ◽  
Eseoghene Valentine Egho ◽  
Joy Akpesiri Egho ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Comparative Advantage ◽  
Ovarian Reserve ◽  
Negative Relationship ◽  
Serum Levels ◽  
Cycle Phase ◽  
Control Group ◽  
Menstrual Cycles ◽  
Elisa Technique ◽  
Determining Factor

Objective: Anti-Mullerian hormone has been indicated as a novel biomarker for ovarian reserve assessment. This study aimed to determine the comparative advantage of serum levels of AMH, FSH, LH, E2, and  LH/FSH ratio among women with varying menstrual cycles and duration of menstruation.   Material and Method: A total of 90 subjects, which consisted of sixty subjects and thirty healthy subjects as control, were recruited. Blood samples were collected on day 3 of the menstrual cycle and evaluated for ovarian markers using the ELISA technique. All data were analyzed using SPSS version 23.0. Results: AMH and LH/FSH levels were lower in women with varying menstrual cycles than in the control group. FSH, LH, and E2 levels were significantly higher in women with varying menstrual cycles and flow duration than in the control group(P<0.05).  AMH was negatively correlated with age (r= -0.72), BMI( r= -0.4), FSH(r= - 0.3), LH(r = -0.2) (p<0.05) and E2(r= -0.2, p>0.05). Also age was positively correlated with FSH(r=0.5,p<0.05), E2(r=0.3, p<0.05) and BMI(r=0.4, p<0.05). The level of AMH was not significant with cycle length and days of flow(p>0.05). This implies that AMH can be measured independently of the cycle phase. This show that AMH was lower in women with varying menstrual cycle with an increase in gonadotrophin and E2. The strong negative relationship between age and AMH implies that age is determining factor of ovarian reserve.  Conclusion: AMH combined with age and FSH may improve ovarian reserve evaluation, making AMH a better marker

scholarly journals Association of VEGF +405 C > G (rs2010963) polymorphism with susceptibility of metabolic syndrome: Cardio-metabolic risk factors and serum Matrix Metaloproteinase-3 concentrations

2021 ◽  
Vol 19 (3) ◽  
pp. 263-271
Author(s):  
Leila Nikniaz ◽  
Mahdieh Abbasalizad-Farhangi
Keyword(s):  
Risk Factors ◽  
Metabolic Syndrome ◽  
Gene Polymorphism ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Control Group ◽  
Vegf Gene ◽  
Pcr Rflp ◽  
Elisa Technique ◽  
Vegf Gene Polymorphism

BACKGROUND: In the present study we investigated the role of +405 VEGF gene polymorphism in the pathogenesis of metabolic syndrome and to explore its association with several biochemical risk factors. MATERIALS AND METHODS: VEGF +405 single nucleotide polymorphism were genotyped in 150 patients with metabolic syndrome and 50 healthy individuals using the PCR-RFLP method. Serum levels of biochemical variables were assessed by commercial ELISA technique. RESULTS: GC genotype was more prevalent among patients with metabolic syndrome. In GC genotype, patients with metabolic syndrome had higher waist to hip ratio, WHR, triglyceride, and lower high density lipoprotein and alanine aminotransferase concentrations compared with the control group. CONCLUSIONS: The current study demonstrated that +405 VEGF gene polymorphism was a potent predictor of metabolic abnormalities in patients with metabolic syndrome. Further studies with larger sample size are needed to clarify these associations properly.

scholarly journals Association of maternal plasma or serum zinc concentration with the birth weight of the child

2018 ◽  
Vol 8 (1) ◽  
pp. 219
Author(s):  
Girija C. Bellad ◽  
Laxmi K. S.
Keyword(s):  
Birth Weight ◽  
Zinc Concentration ◽  
Serum Zinc ◽  
Serum Levels ◽  
Maternal Plasma ◽  
Maternal Serum ◽  
Control Group ◽  
Study Group ◽  
Serum Zinc Concentration ◽  
Zinc Levels

Background: Zinc is a divalent cation first isolated in 1509 and since then its importance has been increasingly recognised in human health and nutrition. Present study has been undertaken to confirm the association between maternal plasma or serum zinc concentration and birth weight.Methods: Present study was cross-sectional study conducted in tertiary care hospital. Newborns with a birth weight of < 2.5kg and their mothers labelled as study group while newborns with a birth weight of > 2.5kg and their mothers were considered as control group. The analysis for the serum concentration of zinc was done using the Atomic Absorption Spectrophotometric method (AAS), this method analytically the most reliable for routine assessment of serum zinc levels. The results deduced from the analysis were accordingly grouped to the study group / control group. We had a sub-group analysis based on the gestational age, as Preterm and Term.Results: Maternal serum levels of zinc 67.04 μg /dl±16.66, in the study group, was less as compared to the maternal serum levels of zinc in the control group with a mean level of 75.39 μg/dl±17.89. The results show a significant association between zinc levels and birth weight. Mean Maternal serum levels of zinc 67.04 μg /dl±16.66, in the study group, was less as compared to the maternal serum levels of zinc in the control group with a mean level of 75.39 μg/dl±17.89. The results show a significant association between zinc levels and birth weight.Conclusions: Present study showed a positive correlation of maternal zinc levels with birth weight and prematurity. Hence, we recommend routine zinc supplementation could be included with other nutritional supplements during pregnancy.

scholarly journals Cytokine profile in adolescent girls with candidal vulvovaginitis

2013 ◽  
Vol 94 (6) ◽  
pp. 817-820 ◽  
Author(s):  
U Kh Ziyadullaev
Keyword(s):  
Inflammatory Response ◽  
Adolescent Girls ◽  
Interleukin 6 ◽  
Serum Levels ◽  
Interleukin 8 ◽  
Yeast Cells ◽  
World Health ◽  
Vaginal Candidiasis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group

Aim. To establish the pathogenic value of serum cytokine (interleukin-6 and interleukin-8) production in patients with candidal vulvovaginitis to optimize diagnostic and therapeutic approaches. Methods. 74 adolescent girls suffering from candidal vulvovaginitis aged from 12 to 19 years were examined. The diagnosis of candidal vulvovaginitis was verified according to the international classification of World Health Organization (ICD-X codes B37.3 and N77.1). At the stage of clinical examination, medical and general history was analyzed, general and gynecological examination was performed. The diagnosis of candidiasis was confirmed by the presence of vegetative forms of Candida spp. (budding yeast cells, pseudomycelium and/or mycelium) in Gram-stained smears from the mucous membranes of the vulva, urethra. The control group consisted of 20 healthy patients of appropriate age and sex. Serum levels of interleukin-6 and interleukin-8 were measured by enzyme-linked immunosorbent assay using «ELISA interleukin-6» and «ELISA interleukin-8» test systems (ZAO «Vector-Best», Russia). Results. The study has shown increased serum levels of interleukin-6 in adolescent patients with candidal vulvovaginitis. Interleukin-6 level in peripheral blood was significantly higher in patients with candidal vulvovaginitis (21.7±2.4 pg/ml) compared to controls (9.4±1.5 pg/ml, p 0.02). Serum interleukin-8 level was also significantly higher (51.2±2.1 pg/ml) in patients with candidal vulvovaginitis compared to controls (23.9±1.04 pg/ml, p 0.01). Conclusion. The revealed features of interleukin-6 and interleukin-8 production may be used as additional objective criteria of the orientation of the inflammatory response in patients with various forms of vaginal candidiasis. The changes of the studied parameters might reflect the state of the immune system and the severity of the inflammatory response.

scholarly journals Comparison of Host Cytokine Response in Piglets Infected With Toxigenic and Non-toxigenic Staphylococcus hyicus

2021 ◽  
Vol 8 ◽  
Author(s):  
Yan Li ◽  
Hongchao Gou ◽  
Pinpin Chu ◽  
Kunli Zhang ◽  
Zhiyong Jiang ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Inflammatory Cytokine ◽  
Transforming Growth Factor ◽  
Clinical Signs ◽  
Serum Levels ◽  
Cytokine Response ◽  
Control Group ◽  
Post Inoculation ◽  
Toxigenic Strain ◽  
Anti Inflammatory

Staphylococcus hyicus is the most common causative agent of exudative epidermitis (EE) in piglets. Staphylococcus hyicus can be grouped into toxigenic and non-toxigenic strains based on its ability to cause EE in pigs. However, the inflammatory response of piglets infected with toxigenic and non-toxigenic S. hyicus has not been elucidated. In this study, we evaluated the serum cytokine profile in piglets inoculated with toxigenic and non-toxigenic S. hyicus strains and recorded the clinical signs in piglets. Fifteen piglets were divided into three groups (n = 5) and inoculated with a toxigenic strain (ZC-4), a non-toxigenic strain (CF-1), and PBS (control), respectively. The changes in serum levels of cytokines (interleukin [IL]-1β, IL-4, IL-6, IL-8, IL-10, IL-12, granulocyte-macrophage colony-stimulating factor, interferon-γ, transforming growth factor-β1, and tumor necrosis factor-α) were evaluated using a cytokine array at 6, 24, 48, and 72 h post inoculation. The results showed that piglets infected with the toxigenic strain exhibited more severe clinical signs and higher mortality than those infected with the non-toxigenic strain. The serum levels of pro-inflammatory cytokine IL-1β were significantly increased in toxigenic-and non-toxigenic-strain-infected piglets compared to those in the control group (p &lt; 0.05), while the anti-inflammatory cytokine IL-10 was significantly up-regulated only in toxigenic group than in control group (p &lt; 0.05). These results indicated that piglets infected with toxigenic and non-toxigenic S. hyicus showed differential infection status and inflammatory responses. Both toxigenic- and non-toxigenic- S. hyicus infection could induce a pro-inflammatory reaction in piglets. In addition, the toxigenic strain induced a strong anti-inflammatory response in piglets as indicated by the increased serum level of IL-10, which may be associated with the severe clinical signs and increased mortality and may be the key cytokine response responsible for pathogenic mechanisms of S. hyicus.

scholarly journals Only serum pepsinogen I and pepsinogen I/II ratio are specific and sensitive biomarkers for screening of gastric cancer

2019 ◽  
Vol 10 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Farahnaz Joukar ◽  
Massood Baghaee ◽  
Masood Sepehrimanesh ◽  
Amineh Hojati
Keyword(s):  
Gastric Cancer ◽  
Plasma Levels ◽  
Serum Levels ◽  
Normal Weight ◽  
Control Group ◽  
Case Group ◽  
Serum Pepsinogen ◽  
Lower Plasma ◽  
Pepsinogen I ◽  
Pepsinogen Ii

AbstractPurposeWe aimed to determine optimal cut-off points of plasma levels of ghrelin and serum levels of pepsinogen I, II, and their ratio for screening of gastric cancer (GC).MethodsBlood samples were taken from 41 patients with confirmed gastric cancer along with 82 patients without malignancy. Serum levels of pepsinogen I and II, plus plasma levels of acylated ghrelin were measured using commercial ELISA kits.ResultsThe case group had significant lower plasma levels of ghrelin, pepsinogen I, and pepsinogen I/II ratio in comparison to the control group (P<0.001). In the control group, there was significant higher serum pepsinogen I (P=0.028) and pepsinogen II (P=0.003) and lower pepsinogen I/II ratio (P=0.020) in males versus females; significantly higher serum pepsinogen II (P=0.047) and lower pepsinogen I/II ratio (P=0.030) in overweight compared to normal weight patients; and significantly lower pepsinogen I/II ratio (P=0.030) in smokers versus non-smoker. In the case group, there was only significantly lower pepsinogen I (P=0.006) in males versus females, and significantly lower plasma ghrelin (P=0.017) in overweight compared to normal weight patients. The characteristic curve analysis indicated that pepsinogen I at a cut-off of 70.95 μg/L and pepsinogen I/II ratio at cut-off of 2.99, had good sensitivity and specificity.ConclusionsJust serums levels of pepsinogen I and the ratio of pepsinogen I/II can be used as biomarker to screen GC.

The impact of different biocompatible coated cardiopulmonary bypass circuits on inflammatory response and oxidative stress

Perfusion ◽  
2009 ◽  
Vol 24 (4) ◽  
pp. 231-237 ◽  
Author(s):  
N. Sohn ◽  
J. Marcoux ◽  
T. Mycyk ◽  
J. Krahn ◽  
QH Meng
Keyword(s):  
Oxidative Stress ◽  
Cardiopulmonary Bypass ◽  
Inflammatory Response ◽  
Inflammatory Responses ◽  
Artery Bypass ◽  
Serum Levels ◽  
Control Group ◽  
Significant Difference ◽  
The Impact ◽  
And Oxidative Stress

This study was to compare the impact of different biocompatible coated circuits on inflammatory response and oxidative stress induced during cardiopulmonary bypass (CPB). Seventy-eight patients undergoing elective coronary artery bypass grafting (CABG) with CPB were randomly assigned to five groups with different biocompatible coated circuits: Trillium, Bioline, Phosphorylcholine, Polymethoxyethyl acrylate (PMEA), and the uncoated control group. Blood was drawn at three different time points: before CPB, 6 and 72 hours post CPB. Unlike the Trillium group, serum levels of TNF-α in the Bioline and Phosphorylcholine groups significantly increased only at 72 hours post CPB (p < 0.05). Serum levels of IL-6 significantly increased at 6 and 72 hours post CPB in all groups (p < 0.01). The Trillium group showed a significant increase of IL-10 compared to the control group at 72 hours post CPB (p < 0.05). Serum levels of NOx in the Phosphorylcholine group significantly decreased at 6 hours post CPB compared to baseline (p < 0.05). Both the Bioline and Phosphorylcholine groups showed statistical decreases in serum NOx levels compared with other groups at 6 hours post CPB (p < 0.05). A significant difference in NOx levels between the Bioline and the control group was also observed at 72 hours post CPB. Myeloperoxidase levels were significantly elevated at 6 and 72 hours post CPB in all groups (p < 0.05). Inflammatory response and oxidative stress are elevated during CABG with CPB. Heparin-coated and the Phosphorylcholine-coated circuits induce less inflammatory responses and oxidative stress compared to other circuits.

scholarly journals Enhancing early breast milk for sick and preterm neonates admitted at regional referral hospitals in Dar es Salaam, Tanzania

2020 ◽  
Author(s):  
Regina Hyera ◽  
Evelyne Assenga ◽  
Theodore Kazimoto ◽  
Germana Leyna ◽  
Francis Mchomvu ◽  
...  
Keyword(s):  
Birth Weight ◽  
Breast Milk ◽  
Neonatal Mortality ◽  
Enteral Feeding ◽  
Intervention Group ◽  
Dar Es Salaam ◽  
Preterm Neonates ◽  
Control Group ◽  
The Mean ◽  
Milk Feeding

Abstract Background: Feeding is a cornerstone in the management of sick or preterm neonates and early initiation of breastfeeding is the single most effective intervention associated with reduced neonatal mortality. However, enteral feeding is often delayed due to haemodynamic instability and the perceived risk of necrotizing enterocolitis (NEC). Alternative routes for early breast milk administration are either oropharyngeal colostrum or minimal enteral nutrition. One strategy to overcome delays in enteral feeds is the implementation of standardized feeding guidelines.Methods: This study was conducted in three regional hospitals in Dar es Salaam, consisting of a historical control group at baseline and an intervention group after implementation of locally developed feeding guidelines. Neonates were consecutively recruited and followed to a maximum of 28 days of life. The controls received routine standard care, while the intervention group received early enteral feeding. The outcomes measured were NEC, time to regain birth weight and neonatal mortality. Odds ratio was used to determine the association between feeding and outcomes, statistical significance was considered when p-value ≤0.05.Results: 292 neonates were enrolled in this study, 163 (55.8%) controls at baseline and 129 (44.2%) after the intervention. Study participants comprised of 130 (44.5%) neonates with very low birth weight and 162 (55.5%) with hypoxic ischemic encephalopathy. The mean age of initiating feeding was 45.34 ± 21.58 SD hours amongst the controls and 8.43 ± 3.02 SD hours in the intervention group. Overall, 1.4% (4 cases) of the neonates were diagnosed with NEC, 1.8% (3 cases) in the control group and 0.8 % (1 case) in the intervention group (P=0.4; 95% CI: 0.25-23.3). The mean duration of regaining birth weight was significantly reduced from 11.26 ± 4.34 SD days among controls to 8.25 ± 2.96 SD days in the intervention group (p=0.000; 95% CI: 1.61 - 4.41). Mortality in this high-risk groups of neonates remained high (40.8%), without significant difference between the control (41.7%) and intervention group (39.5%). Conclusions: Neonates who received early breast milk feeding following the implementation of feeding guidelines significantly regained birth weight earlier. No association was demonstrated between early breast milk feeding and increased NEC or mortality.

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