Assessment of the Antioxidant Effect of Ethanolic Extracts Obtained from Agrimonia eupatoria L., Filipendula ulmaria (L.) Maxim. and Filipendula vulgaris Moench Collected from the Estern Part of Romania

Numerous plants are currently used for both their curative and preventive effects in a wide range of pathologies. They possess multiple beneficial effects, including the well-known antioxidant capacity. Agrimonia eupatoria L. and Filipendula spp., from the Rosaceae family have emerged as a potential source of phenolic constituents, showing antioxidant activities. The aim of this study was to obtain ethanolic extracts from Agrimonia eupatoria L., Filipendula ulmaria (L.) Maxim. and Filipendula vulgaris Moench. collected from the West part of Romania and to determine their antioxidant capacity. Two DPPH assays were used to determine the antioxidant activity of the extracts, ethanolic solution of 1 and 0.1 mM DPPH. Our data indicated that all the extracts, regardless of the place of harvest possess significant antioxidant activity. The most potent activity was recorded for the extracts prepared from Agrimonia eupatoria L. collected from Bazia� � Locva Mountains in mixture with ethanolic solution of 0.1 mM DPPH, followed by Filipendula ulmaria (L.) Maxim, using the same concentration of ethanolic solution of standard antioxidant.

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Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

Biomedicines ◽

10.3390/biomedicines9030294 ◽

2021 ◽

Vol 9 (3) ◽

pp. 294

Author(s):

Raffaele Pugliese ◽

Anna Arnoldi ◽

Carmen Lammi

Keyword(s):

Mechanical Properties ◽

Antioxidant Activity ◽

Self Assembly ◽

Antioxidant Activities ◽

Functional Materials ◽

Cd Spectroscopy ◽

Beneficial Effects ◽

Naturally Occurring ◽

New Research ◽

Peptide Hydrogel

Naturally occurring food peptides are frequently used in the life sciences due to their beneficial effects through their impact on specific biochemical pathways. Furthermore, they are often leveraged for applications in areas as diverse as bioengineering, medicine, agriculture, and even fashion. However, progress toward understanding their self-assembling properties as functional materials are often hindered by their long aromatic and charged residue-enriched sequences encrypted in the parent protein sequence. In this study, we elucidate the nanostructure and the hierarchical self-assembly propensity of a lupin-derived peptide which belongs to the α-conglutin (11S globulin, legumin-like protein), with a straightforward N-terminal biotinylated oligoglycine tag-based methodology for controlling the nanostructures, biomechanics, and biological features. Extensive characterization was performed via Circular Dichroism (CD) spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), rheological measurements, and Atomic Force Microscopy (AFM) analyses. By using the biotin tag, we obtained a thixotropic lupin-derived peptide hydrogel (named BT13) with tunable mechanical properties (from 2 to 11 kPa), without impairing its spontaneous formation of β-sheet secondary structures. Lastly, we demonstrated that this hydrogel has antioxidant activity. Altogether, our findings address multiple challenges associated with the development of naturally occurring food peptide-based hydrogels, offering a new tool to both fine tune the mechanical properties and tailor the antioxidant activities, providing new research directions across food chemistry, biochemistry, and bioengineering.

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Assessment of In Vitro Bioaccessibility of Polyphenols from Annurca, Limoncella, Red Delicious, and Golden Delicious Apples Using a Sequential Enzymatic Digestion Model

Antioxidants ◽

10.3390/antiox10040541 ◽

2021 ◽

Vol 10 (4) ◽

pp. 541

Author(s):

Giulia Graziani ◽

Anna Gaspari ◽

Claudio Di Vaio ◽

Aurora Cirillo ◽

Carolina Liana Ronca ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

In Vitro Digestion ◽

Enzymatic Digestion ◽

Beneficial Effects ◽

Golden Delicious ◽

In Vitro Bioaccessibility ◽

Inflammatory Processes ◽

Great Relevance

Four different varieties of apples have been considered (Limoncella, Annurca, Red Delicious, and Golden Delicious) to estimate the extent of colon polyphenolics release after in vitro sequential enzyme digestion. Since several studies report a positive effect of apple polyphenols in colonic damage, we found of interest to investigate the colon release of polyphenols in different varieties of apples in order to assess their prevention of colonic damage. UHPLC-HRMS analysis and antioxidant activity (ABTS, DPPH, and FRAP assays) were carried out on the apple extracts (peel, flesh, and whole fruit) obtained from not digested samples and on bioaccessible fractions (duodenal and colon bioaccessible fractions) after in vitro digestion. Polyphenolic content and antioxidant activities were found to vary significantly among the tested cultivars with Limoncella showing the highest polyphenol content accompanied by an excellent antioxidant activity in both flesh and whole fruit. The overall trend of soluble antioxidant capacity from the soluble duodenal phase (SDP) and soluble colonic phase (SCP) followed the concentrations of flavanols, procyandinis, and hydroxycinnamic acids under the same digestive steps. Our results highlighted that on average 64.2% of the total soluble antioxidant activity was released in the SCP with Limoncella exhibiting the highest values (82.31, 70.05, and 65.5%, respectively for whole fruit, flesh, and peel). This result suggested that enzymatic treatment with pronase E and viscozyme L, to reproduce biochemical conditions occurring in the colon, is effective for breaking the dietary fiber-polyphenols interactions and for the release of polyphenols which can exercise their beneficial effects in the colon. The beneficial effects related to the Limoncella consumption could thus be of potential great relevance to counteract the adverse effects of pro-oxidant and inflammatory processes on intestinal cells.

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Utero-relaxing and anti-free-radical effects of hydro-ethanolic extracts of Desmodium ramosissimum in the wistar female rats

10.25259/jrhm_33_2020 ◽

2021 ◽

Vol 2 ◽

pp. 6

Author(s):

Hèzouwè Madjalani ◽

Kossi Metowogo ◽

Tcha Pakoussi ◽

Aklesso Nabede ◽

Essodjolon Kanabiya ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Medicinal Plants ◽

Rural Areas ◽

Public Health Problem ◽

Stem Bark ◽

Sub Saharan Africa ◽

Female Rats ◽

Phenolic Constituents ◽

Ethanolic Extracts

Objectives: In sub-Saharan Africa, the therapeutic use of medicinal plants is of arousing interest. Reproductive disorders are a public health problem like everywhere else in the world. The population, especially in rural areas, uses medicinal plants for their beneficial effects. In Africa, the population uses these medicinal plants for their healthcare, including solving problems related to childbirth where there is an increase in the number of women known as “traditional birth attendants.” The effects of its medicinal plants relate to the various reproductive processes in women, from pregnancy to induction of labor, elimination of placental retention, and management of postpartum hemorrhage. To contribute to the enhancement of medicinal plants traditionally used in reproductive healthcare in Togo and to make information on their pharmacological effects available to the population, this work focused on the evaluation utero-relaxing and anti-free radical effects of extracts of Desmodium ramosissimum in the female rats of the Wistar strain. Material and Methods: The effects of hydro-ethanolic extracts of leaves, stem barks, and roots of D.ramosissimum were then tested on shreds Myometer of the female rats mounted in an organ vat and the antioxidant activity of these extracts tested was then determined. Results: The results show that hydro-ethanolic extracts from leaves, stem bark, and roots of D.ramosissimum cause a reduction in the frequency of spontaneous contractions of the uterine muscle. This reduction shows that these extracts have relaxing effects on the contraction of this muscle. The leaves extract showed better antioxidant activity compared to other extracts. Conclusion: This antioxidant activity is probably due to the phenolic constituents and its metabolites would be partly responsible for the pharmacological activities of D.ramosissimum. D.ramosissimum could thus be used to reduce uterine contractions in order to avoid Threats of Premature Labor.

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Anti-urease, total phenolic content and antioxidant activities of some Inula l. (Asteraceae) taxa in Turkey

Genetika ◽

10.2298/gensr2003825s ◽

2020 ◽

Vol 52 (3) ◽

pp. 825-834

Author(s):

Emre Sevindik ◽

Sinem Aydin ◽

Mehmet Paksoy ◽

Bahar Sokmen

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Soxhlet Extraction ◽

Total Phenolic ◽

Food Industries ◽

Total Antioxidant ◽

Ethanol Extracts

The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54?0.002 ?g GAE/mL) and I. macrocephala (189.16?0.002 ?g AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.

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Antioxidant and cytotoxic studies of Acacia nilotica twig extract and their green synthesized silver nanoparticles

Letters in Applied NanoBioScience ◽

10.33263/lianbs92.975980 ◽

2020 ◽

Vol 9 (2) ◽

pp. 975-980 ◽

Cited By ~ 1

Keyword(s):

Antioxidant Activity ◽

Silver Nanoparticles ◽

Cell Lines ◽

Antioxidant Activities ◽

Reducing Power ◽

Acacia Nilotica ◽

Total Phenolic ◽

Hek 293 ◽

Selected Area Electron Diffraction ◽

Wide Range

Acacia nilotica (L.) Delile is well known as “Desi Kikar”or Babul in India that possesses a wide range of pharmacological activities. In the present study, Acacia nilotica twig extract and its synthesized silver nanoparticles (AgNPs) were evaluated for total phenolic content (TPC), antioxidant activity and cytotoxic effects. Characterization of AgNPs was done by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Selected area electron diffraction (SAED) techniques. Antioxidant potential was determined using different assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power and β-carotene linoleic acid. Cytotoxicity was tested by 3-(4,5-dimethyl-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay on Human embryonic kidney (HEK)-293 cell lines. The results indicated that AgNPs exhibited higher antioxidant activity (81.11 %) and TPC (57.35 mg of GAE/mL of extract) as compare to plant extract. A positive correlation was observed between the TPC and antioxidant activities. The inhibitory concentration (IC50) of A. nilotica extract and AgNPs was 52.08µg/mL and 56.82µg/mL respectively. Cytotoxicity against HEK-293 cell lines was dose dependent. Accordingly, it is summarized that A. nilotica based AgNPs could serve as a potential antioxidant for therapeutic purposes.

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Mycochemical composition and antioxidant potential of Macrolepiota africana (Heim) Heinem. (Agaricaceae), an edible mushroom from Gabon

Open Access Research Journal of Life Sciences ◽

10.53022/oarjls.2021.2.1.0134 ◽

2021 ◽

Vol 2 (1) ◽

pp. 028-033

Author(s):

Hugues Calixte Eyi Ndong ◽

André Ledoux Njouonkou

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Activity Index ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Edible Mushroom ◽

Mushroom Species ◽

Chemical Screening ◽

Ethanolic Extracts ◽

Pharmacological Potential

Macrolepiota africana is a fungus used as food in Gabon for its culinary properties. However, its nutritional value, mycochemical contents, antioxidant properties and health potential still unknown. The present study investigated the phytochemicals and antioxidant properties of this mushroom species. This chemical screening was followed by a study of the antioxidant activity and a prediction of additional pharmacological activities of M. africana. Using standard methodology, the mycochemical analyses were carried out on aqueous, hydro-ethanolic and ethanolic fungi extracts. The antioxidant activity of the mushroom extracts was determined using DPPH radical scavenging assay. Apart from Digitoxigenine, flavonol and gitoxigenine that were not found in any of the extracts, all other tested mycochemical were found in atleast one of the extracts. Alkaloids, flavonoids, polyphenols, oses and holosides, proanthocyanidins and coumarins were found in all extracts at different intensity. Saponosids, sterols and triterpenes, tannins gallics, reducing sugar, anthracenosides and digitoxine were found in two extracts while tannins catechics, flavone and gitoxine were detected only in one extract. The dosage of phenolic compounds confirmed the richness of this fungus in total polyphenols, its moderate richness in proanthocyanidins, the lack of flavonoids in the aqueous extract and the moderate richness of the hydro-ethanolic and ethanolic extracts in flavonoids. Regarding the antioxidant activities, the results obtained for the DPPH trapping test showed that the different extracts had low to moderate antioxidant activity with antioxidant activity index (IAA) ranged 0.29 to 0.97 respectively in aqueous and ethanolic extracts. The presence of these mycochemical compounds along with the identified antioxidant activities shows that this M. africana have some pharmacological potential.

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Comparative Study of the Chemical Compositions and Antioxidant Activities of Fresh Juices from Romanian Cucurbitaceae Varieties

Molecules ◽

10.3390/molecules25225468 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5468

Author(s):

Anna Cazanevscaia Busuioc ◽

Andreea-Veronica Dediu Botezatu ◽

Bianca Furdui ◽

Costel Vinatoru ◽

Filippo Maggi ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

High Performance ◽

Antioxidant Activities ◽

Fruit Juices ◽

Antioxidant Potential ◽

Chemical Compositions ◽

Beneficial Effects ◽

Benincasa Hispida ◽

First Time

Cucurbitaceae is a family of health-promoting plants due to their compounds with beneficial effects. The aim of this study was to analyze, for the first time, the chemical composition, the antioxidant activity and the metal chelating properties of fruit juices obtained from four different species of the Cucurbitaceae family cultivated in Romania, namely Momordica charantia, Cucumis metuliferus, Benincasa hispida and Trichosanthes cucumerina. The samples of juice were analyzed by high-performance liquid chromatography (HPLC) and all the four species displayed high levels of the two triterpenes, oleanolic and ursolic acids, and also in phenolic compounds, including catechin, (−)-epicatechin and gallic acid. The juices demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (ranging from 20 to 95%,), a good iron binding ability (ranging from 7.45 ± 0.28% to 86.95 ± 0.97%) and also promising antioxidant potential against the ABTS radical (ranging from 4.97 to 32.60 μETx/mL juice). Our findings raise interesting questions for further research on Cucurbitaceae fruit juices and, consequently, their very good antioxidant potential suggests these fruits should be further explored for their protective effect against oxidative damage. This is the first time the chemical composition and antioxidant activities of fruit juices from these four Romanian Cucurbitaceae varieties have been investigated.

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Evaluation of Antioxidant Capacity ofSolanum sessiliflorum(Cubiu) Extract: AnIn VitroAssay

Journal of Nutrition and Metabolism ◽

10.1155/2015/364185 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

Diego Rocha de Lucena Herrera Mascato ◽

Janice B. Monteiro ◽

Michele M. Passarinho ◽

Denise Morais Lopes Galeno ◽

Rubén J. Cruz ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Antioxidant Activities ◽

Flavonoid Glycosides ◽

Specific Staining ◽

Native Populations ◽

Chemical Groups ◽

Solanaceae Family ◽

Hydroethanolic Extract

Cubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We performed a phytochemical screening to identify the main chemical groups that could confer antioxidant activity to this extract. Several tests and qualitative precipitation specific staining for major classes of secondary metabolites were used. Antioxidant capacityin vitrotests (DPPH and ABTS) were also used to assess the extract’s ability to sequester free radicals of 70% hydroethanolic and aqueous extracts of cubiu flour. Alkaloids, organic acids, phenols, flavonoid glycosides, and coumarins were found in the hydroethanolic extract while the aqueous extract presented anthocyanins, gums, tannins and mucilage, amino groups, and volatile and fixed acids. Forin vitrotests, theIC50value obtained in the DPPH assay was 606.3 ± 3.5 μg/mL while that for the ABTS assay was 290.3 ± 10.7 µg/mL. Although cubiu extracts present chemical compounds directly related to antioxidant activity, our results show that it has a low antioxidant activity. Additional studies will be needed to isolate and characterize specific compounds to further assess antioxidant activity.

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The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

Asian Food Science Journal ◽

10.9734/afsj/2021/v20i430291 ◽

2021 ◽

pp. 106-120

Author(s):

Zamree Md Shah ◽

Mohd Kamal Nik Hasan ◽

Khairul Kamilah Abdul Kadir ◽

Mohd Shahidan Mohd Arshad ◽

Zulkhairi Amom

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Pharmaceutical Preparations ◽

Extraction Yield ◽

Extraction Time ◽

Extraction Temperature ◽

Solid Ratio ◽

Beneficial Effects ◽

High Antioxidant Activity ◽

Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

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Bioactivities of plant extracts collected in Halmahera, Indonesia: A bioprospection study of underexplored plant species

INDONESIAN JOURNAL OF PHARMACY ◽

10.22146/ijp.1774 ◽

2021 ◽

pp. 376-384

Author(s):

Marlin Megalestin Raunsai ◽

Kartika Dyah Palupi ◽

Ahmad Fathoni ◽

Andria Agusta

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Plant Species ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Total Phenolic ◽

Antioxidant Agents ◽

Ethanolic Extracts ◽

Potential Sources ◽

Weak Antibacterial Activity

The discovery of new antibiotics to overcome the growing resistance problem as well as the discovery of new natural, safe antioxidants to combat oxidative stress are still urgently needed. Medicinal plants are known to produce potential therapeutic substances which are more biologically selective than synthetic compounds. Therefore, we explored the bioactivities of 35 ethanolic extracts from 24 underexplored plant species collected in Halmahera, to find potential sources for antibacterial and antioxidant agents. Dried plant parts were extracted using ethanol 96%. Thin layer chromatography-direct-bioautography (TLC-DB) and minimum inhibitory concentration (MIC) determination were used to evaluate the antibacterial effect. Antioxidant activity was determined against DPPH using TLC-DB and microdilution assay. Total phenolic content (TPC) was determined using Folin-Ciocalteu’s method. The ethanolic extracts exhibited moderate to weak antibacterial activity against Escherichia coli and Staphylococcus aureus. However, the leaf extract of Elaeocarpus dolichostylus, Elaeocarpus multiflorus, and Psychotria celebica as well as the stem bark extract of Elaeocarpus dolichostylus, Cinnamomum sintoc, and Garcinia latissima displayed very strong antioxidant activities against DPPH with AAI values between 4.60 to 13.42. A strong correlation between TPC and antioxidant activity with r = 0.8712 was observed. Despite the moderate to weak antibacterial activity, eight underexplored plant species exhibit strong antioxidant activities. A high correlation between TPC and antioxidant activity indicating a prominent role of phenolic compounds in the plants’ antioxidant properties. These findings indicate that collected plants from Halmahera are potential to be studied and developed further as the potential sources for novel antioxidants.

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