scholarly journals EVALUATION OF CHONDROPROTECTIVE EFFICACY OF TRIAMCINOLONE IN OSTEOARTHRITIS INDUCED RAT MODEL

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Noaman Ishaq ◽  
Quratulain Mehdi ◽  
Novera Sohail Bajwa ◽  
Shabana Ali ◽  
Bushra Shaheen ◽  
...  
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Cruciate Ligament ◽  
Gait Pattern ◽  
Control Group ◽  
P Value ◽  
Surgical Removal ◽  
Sprague Dawley ◽  
Medical College ◽  
Group I

ABSTRACT BACKGROUND AND OBJECTIVE: Osteoarthritis is one of the most common joint diseases afflicting human, characterized by progressive degeneration of articular cartilage in which chondrocytes fails to adequately repair. Objective of this study is to evaluate the chondroprotection offered by triamcinolone in osteoarthritis induced rat model METHODOLOGY: This Laboratory based experimental study was conducted in Department of Pharmacology, Army Medical College, Rawalpindi, in collaboration with National Institute of health, Islamabad from April-June2019. Osteoarthritis was induced by surgical removal of medial meniscus and anterior cruciate ligament resection in right knee joint of Sixteen (16) anesthetized rats of Sprague Dawley breed. They were divided in two (02) groups with eight (08) rats in each group. Group I was disease control in which 0.2 ml Intra articular saline was administered for three weeks. While group II was treatment group that was treated by 70 µl intra articular triamcinolone once weekly for three weeks. After that gait pattern of rats was scored. Animals were euthanized with over dosage of inhaled chloroform and sample of proximal tibia was taken for histopathological analysis.RESULTS: Mean gait score of control group and treatment group was 3.25±.707 and 2.25±.463 with a p value of .028 that is statistically significant. While mean histopathological modified Mankin score of control and treatment group was 11.5±1.195 and 8.5±1.195 respectively with a significant P-value of <0.01. CONCLUSION: Intra articular administration of triamcinolone in Osteoarthritis induced rats resulted in improvement in gait pattern and histopathology. Keywords: Chondroprotective efficacy, Osteoarthritis, Triamcinolone.

scholarly journals EVALUATION OF CHONDROPROTECTIVE EFFECTS OF HYALURONIC ACID IN A RAT MODEL OF OSTEOARTHRITIS

2022 ◽  
Vol 71 (6) ◽  
pp. 2189-93
Author(s):  
Noaman Ishaq ◽  
Shabana Ali ◽  
Muhammad Waqar Aslam Khan ◽  
Kulsoom Farhat ◽  
Nausheen Ata ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Treatment Group ◽  
Rat Model ◽  
Cruciate Ligament ◽  
Gait Pattern ◽  
Control Group ◽  
P Value ◽  
Army Medical ◽  
Medical College ◽  
Group I

Objective: To evaluate the chondroprotective effects of hyaluronic acid in a rat model of osteoarthritis. Study Design: Laboratory based experimental study. Place and Duration of Study: Department of Pharmacology, Army Medical College, Rawalpindi, in alliance with National Institute of health, Islamabad and Department of Pathology, Army Medical College Rawalpindi, from Apr to Jun 2019. Methodology: Sixteen (16) rats of Sprague Dawley breed were procured in this study. Osteoarthritis was induced in right knee joint of rats by surgical resection of medial meniscus and anterior cruciate ligament. They were allocated into two (02) groups with eight (08) rats in both groups. Group-I was control group that was treated with 0.2 ml intra articular saline once weekly for four weeks. While group-II was treatment group that was intra particularly administered with 0.2ml hyaluronic acid once weekly for four weeks. One week after the last dosage, gait pattern of the animals was scored. Then animals were sacrificed and a part of proximal tibia was obtained for histopathologic analysis. Results: Mean gait score of control group and treatment group was 3.25 ± 0.707 and 1.00 ± 0.756 respectively with a statistically significant p-value of <0.001, while mean histopathological Modified Mankin score of control and treatment group was 11.5 ± 1.195 and 5.50 ± 1.195 respectively with a significant p-value of <0.001. Conclusion: Intra articular viscosupplementation of hyaluronic acid in rat model of osteoarthritis resulted in improved gait pattern and histopathology.

scholarly journals EVALUATION OF CHONDROPROTECTIVE EFFECTS OF INTRA ARTICULAR PIROXICAM IN POST-TRAUMATIC OSTEOARTHRITIS MODEL OF RAT

2021 ◽  
Vol 71 (2) ◽  
pp. 593-97
Author(s):  
Noaman Ishaq ◽  
Shabana Ali ◽  
Qurra Tul Ain Haider ◽  
Muhammad Abdul Basit Qaisrani ◽  
Komal Mumtaz Malik ◽  
...  
Keyword(s):  
Treatment Group ◽  
Cruciate Ligament ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Surgical Removal ◽  
Histopathological Analysis ◽  
Sprague Dawley ◽  
Group I ◽  
Post Traumatic

Objective: To evaluate the chondroprotective effect of piroxicam in post-traumatic osteoarthritis model of rat. Study Design: Laboratory based experimental study. Place and Duration of Study: Pharmacology department, Army Medical College, Rawalpindi, from Apr to Jun 2019. Methodology: Project included sixteen rats of Sprague Dawley breed. Osteoarthritis was induced in anesthetized rats by surgical removal of medial meniscus and anterior cruciate ligament resection. After that rats were randomly allocated in two groups with eight rats in each group. Rats of group I were positive control that received 0.2 ml saline intra articularly once weekly for four weeks. Meanwhile rats of group II (treatment group) received 50 µl piroxicam intra articularly once weekly for four weeks. One week after the drug intervention, radiograph of the right knee joint of all rats were taken. Animals were then sacrificed with inhaled chloroform and part of proximal tibia was obtained for histopathological analysis. Results: Comparison of radiographs of both groups depicted a significant p-value of <0.01. Meanwhile mean histopathological score of control group and treatment group were 11.50 ± 1.195 and 6.50 ± 1.195 respectively with a p-value of <0.01. Conclusion: Intra articular administration of piroxicam in post-traumatic Osteoarthritis model of rats resulted in improvement in radiographic grades and histopathology scores.

P3486Experimental model for heart failure in rats: apelin-13/apj and bnp-45 gene expression analysis

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
H R Helmi ◽  
A P Sunjaya ◽  
D Limanan ◽  
A R Prijanti ◽  
S W A Jusman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heart Failure ◽  
Cardiac Hypertrophy ◽  
Mrna Expression ◽  
Rat Model ◽  
Control Group ◽  
P Value ◽  
Sprague Dawley ◽  
Hypoxia Exposure ◽  
Systemic Hypoxia

Abstract Background Apelin, an adipokine peptide and its receptor has recently emerged as a key signaling pathway in maintaining cardiac performance at chronic pressure loads. Apelin has been linked to ventricular dysfunction and therefore maybe of pathophysiologic relevance as a candidate biomarker in HF patients. Purpose This study aims to investigate Apelin-13 gene expression and level, and Apelin receptor (APJ) level in a rat model of heart failure induced by chronic systemic hypoxia and their correlation to BNP-45 gene expression and level, the current gold standard biomarker for heart failure, and to cardiac histopathologic changes. The effect of chronic systemic hypoxia on cardiac hypertrophy, remodeling and heart failure parameters is also of interest. Methods Twenty-eight male Sprague-Dawley rats (8–12 weeks of age) were placed in special hypoxic chambers divided into 7 groups – a control group provided with normoxia (atmospheric O2 levels) and 6 exposure groups exposed to hypoxia (8% O2) for 6 hours, 1, 3, 5, 7 and 14 days respectively prior to measurement. Changes in the expression of Apelin and BNP-45 were measured using quantitative real-time PCR, whereas changes in Apelin-13, APJ and BNP-45 levels were measured using ELISA. Histopathology staining using Hematoxylin and Eosin was performed on cardiac tissues post-termination. Results Compared to control, BNP-45 mRNA expression in the hypoxic heart was only significantly different in day 14, whereas, Apelin mRNA expression had showed significantly higher values starting from day 7 onward. This is in line with the evidence of cardiac hypertrophy based on histopathologic examination present from day 7 onwards. BNP-45 and Apelin-13 levels were significantly higher compared to control from day 5 onwards with a peak on day 7. Although significantly higher than control, Apelin-13 and BNP-45 level decreases in day 14 as compared to day 7. Mean APJ levels showed a similar profile with Apelin-13 and BNP-45 levels with a peak in day 7 (4.619 ng/mL). The cardiac Apelin-13 level shows strong significant correlation with BNP-45 levels (r 0.823, p-value 0.0001). There was also a strong significant correlation between APJ receptor levels with Apelin-13 (r 0.9029, p-value 0.001) and BNP-45 (r 0.9062, p-value 0.0009) levels. Apelin-13, APJ and BNP-45 levels also showed strong significant positive correlation to the duration of hypoxia exposure. Conclusion Chronic (≥5 days) and not acute systemic hypoxia in an experimental rat model leads to increase in Apelin-13, APJ and BNP-45 levels. Apelin-13 and BNP-45 were found to significantly increase from 5 days onwards. Apelin mRNA expression was found to show significant increase earlier compared to BNP-45 mRNA expression. Hence, Apelin may serve as a new candidate biomarker for detection of HF due to oxidative stress compared to BNP-45. Exposure to chronic systemic hypoxia can serve as an easily replicable rat model for heart failure. Acknowledgement/Funding Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tarumanagara University, Jakarta, Indonesia

scholarly journals Intra-Articular Delivery of Quercetin Using Thermosensitive Hydrogel Attenuate Cartilage Degradation in an Osteoarthritis Rat Model

Cartilage ◽  
2018 ◽  
Vol 11 (4) ◽  
pp. 490-499 ◽  
Author(s):  
Sze-Wing Mok ◽  
Sai-Chuen Fu ◽  
Yau-Chuk Cheuk ◽  
I-Ming Chu ◽  
Kai-Ming Chan ◽  
...  
Keyword(s):  
Rat Model ◽  
Release Kinetics ◽  
Cruciate Ligament ◽  
Symptom Relief ◽  
Post Treatment ◽  
Control Group ◽  
Low Grade ◽  
Sprague Dawley ◽  
Thermosensitive Hydrogel

Objective Quercetin (Que), a bioflavonoid, is both anti-inflammatory and antioxidative. Que has been used as an oral supplement for osteoarthritis (OA) with inconsistent findings because of its low bioavailability. We encapsulated Que in a mPEG-polypeptide thermogel to prolong its bioactivity. The efficacy of this formulation was evaluated in a posttraumatic OA rat model. Design Methoxy-poly(ethylene glycol)-l-poly(alanine) (mPEG-PA) polymer was synthesized and characterized in terms of cytotoxicity and release kinetics in vitro. At 12 weeks old, Sprague-Dawley rats underwent anterior cruciate ligament transection (ACLT). At 24 weeks post-operation, rats received either an intra-articular (IA) injection of saline, hydrogel, or hydrogel with Que (50 or 500 μg). Gait analysis was performed at pre-ACLT, pre-treatment, and at 4, 8, and 12 weeks post-treatment. At 12 weeks post-treatment, knee joints were collected for histopathological evaluation. Results In vitro studies showed that chondrocytes were viable after 72 hours of incubation with mPEG-PA, and the release of Que could be sustained for >28 days. Among all OA rats, the limb idleness index (LII) were significantly increased at 24 weeks post-ACLT. Rats that received hydrogel with Que (50 μg) showed the most reduction in LII at both 4 and 8 weeks post-treatment. The Osteoarthritis Research Society International score of rats received hydrogel with Que (50 μg) was significantly lower than the control group. All rats suffered from low-grade synovitis (Krenn score: 2-4). Conclusion This study suggests that a sustained delivery of Que (50 μg) could provide symptom relief and also delay the progression of OA in the knee.

scholarly journals A COMPARISON OF BODY MASS INDEX AND WEIGHT GAIN AMONG MALE SPRAGUE DAWLEY RATS ON BROILER AND DOMESTIC CHICKEN MEAT FEED

2020 ◽  
Vol 70 (6) ◽  
pp. 1662-65
Author(s):  
Hammad Gul Khan ◽  
Amir Rashid ◽  
Zainab Khan ◽  
Faiza Aman ◽  
Muhammad Shoaib
Keyword(s):  
Weight Gain ◽  
Broiler Chicken ◽  
Diet Group ◽  
Chicken Meat ◽  
Control Group ◽  
P Value ◽  
Sprague Dawley ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Pelleted Diet

Objective: To compare and evaluate the effects of broiler chicken fed with commercially offered feed and chicken fed with organic diet on BMI and weight gain in Sprague Dawley rats. Study Design: A randomized controlled trial. Place and Duration of Study: Multi-Disciplinary Laboratory of Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi, and collaborated with Quaid-e-Azam University, Islamabad from November 2017 to April 2019. Methodology: Ninety male early weaned Sprague Dawley rats were arbitrarily assorted into three groups (n=30). Group I control rats were nourished on standard pelleted diet. Group II rats were nourished with organic chicken meat along with their standard pelleted diet. Group III rats were nourished with broiler chicken meat along with their standard pelleted diet. BMI and weight gain were estimated. All variables were calculated as Mean ± SD values. One-way ANOVA was applied to determine the significance among groups followed by Tuckey’s HSD post hoc test. p value ≤ 0.05 was considered significant. Results: The current study demonstrated significant increase in BMI (p ≤ 0.001) and weight gain (p ≤ 0.001) in both experimental groups as compared to control group. Conclusion: Based on the findings of our study we propose that broiler chicken meat consumption could be the probable cause of weight imbalances and out of proportion gain of weight and growth in experimental rats.

scholarly journals Thiosulfinate-Enriched Allium sativum Extract as an Adjunct to Antibiotic Treatment of Sepsis in a Rat Peritonitis Model

2021 ◽  
Vol 11 (11) ◽  
pp. 4760
Author(s):  
Francisco Javier Redondo-Calvo ◽  
Omar Montenegro ◽  
David Padilla-Valverde ◽  
Pedro Villarejo ◽  
Víctor Baladrón ◽  
...  
Keyword(s):  
Treatment Group ◽  
Antibiotic Treatment ◽  
Allium Sativum ◽  
Activity Level ◽  
Control Group ◽  
Sprague Dawley ◽  
E Coli ◽  
Group I ◽  
Group Ii ◽  
Peritonitis Model

Up to now, there are no studies that have shown a decrease in morbidity and mortality in the context of sepsis and septic shock, except for antibiotic therapy and the objective-guided resuscitation strategy. The goal was to evaluate the use of thiosulfinate-enriched Allium sativum extract (TASE) as an adjuvant in the management of sepsis. An experimental in vivo study was carried out with male Sprague Dawley® rats. Animals were randomized in three treatment groups: the control group (I), antibiotic (ceftriaxone) treatment group (II) and ceftriaxone plus TASE treatment group (III). All animals were housed and inoculated with 1 × 1010 CFU/15 mL of intraperitoneal Escherichia coli ATCC 25922. Subsequently, they received a daily treatment according to each group for 7 days. Clinical, analytical, microbiological, and histopathological parameters were evaluated. Statistically significant clinical improvement was observed in the ceftriaxone plus TASE vs. ceftriaxone group in weight, ocular secretions, whiskers separation and physical activity level (p ≤ 0.05). When comparing interleukins on the third day of treatment between II and III, we found statistically significant differences in IL-1 levels (p < 0.05). Blood and peritoneal liquid cultures of group I were positive for multisensitive E. coli. Group II and III cultures were negative for E. coli, although an overgrowth of Enterococcus faecalis was found. In conclusion, TASE used as an adjuvant to antibiotic treatment in the management of sepsis could improve response profiles with sepsis attenuation, thus reducing overall mortality after an animal peritonitis model.

scholarly journals EFFECT OF OBESTATIN ON PITUITARY GONADAL AXIS, LEPTIN AND MDA LEVELS IN OBESE SPRAGUE DAWLEY RATS

2021 ◽  
Vol 71 (3) ◽  
pp. 1061-65
Author(s):  
Nadia Latif ◽  
Amina Rasul ◽  
Sajid Ali ◽  
Rimsha Zia
Keyword(s):  
Obese Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Group Iii ◽  
Army Medical ◽  
Testosterone Levels ◽  
Medical College ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Obese Rats

Objective: To determine the effect of obestatin administration on FSH, LH, testosterone, leptin and MDA levels in obese Sprague Dawley Rats. Study Design: Laboratory based animal study. Place and Duration of Study: Physiology department, Army Medical College Rawalpindi, from Mar to Jun 2015. Methodology: This randomized controlled trial was conducted at Physiology Department Army medical college. Male healthy Sprague Dawley rats were randomly divided into 3 groups (n–15 each) i.e. control group (group I) fed with normal pellet diet (NPD), obese group (group II) and obestatin treated obese group (group III) fed with high fat diet (HFD). After 10 weeks, group III was treated with obestatin (1nmol/100ml intraperitoneally). Blood samples were obtained by terminal intracardiac sampling for bioasssays of FSH, LH, testosterone, leptin and MDA by ELISA. Results: Obestatin supplementation in obese rats showed significant increase in LH levels (3.79 ± 0.05) and testosterone levels (2.07 ± 0.22) when compared to the non treated obese rats (2.19 ± 0.07) and (1.37 ± 0.15) respectively while significant decrease in leptin (3.85 ± 0.23) and MDA levels (1.62 ± 0.07) was observed when compared to the non-treated control groups (6.10 ± 1.18) and (1.95 ± 0.07) respectively. However, serum FSH levels remained unchanged among the treated and nontreated groups. Conclusion: Obestatin increases the testosterone levels by augmenting the pituitary gonadal axis through decrease in the oxidative stress and leptin levels in obese rats.

scholarly journals The Effect of Myrmecodia pendans Ethanol Extract on Inflamed Pulp: Study on Sprague Dawley Rats

2019 ◽  
Vol 3 (2) ◽  
pp. 115
Author(s):  
Janti Sudiono ◽  
Meylisa Hardina
Keyword(s):  
Treatment Group ◽  
Healing Process ◽  
Ethanol Extract ◽  
Flavonoid Compound ◽  
Control Group ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Group Ii

Background: Inflammation is a body response caused by injury and infection. Pulpitis is a pulp tissue inflammation which is the continuous process of pulp hyperemia by bacteria invasion. Myrmecodia pendans or Sarang semut is known to contain flavonoid compound which has the anti inflammation effect. The purpose of this study is to investigate the effect of Myrmecodia pendans ethanol extract on the healing process of pulp inflammation.Materials and Methods: This experimental study involved pre- and post-in vivo treatment of 27 Sprague Dawley rats in which the inducted pulpitis model was obtained by injecting 0.01 mL Porphyromonas gingivalis into the dental pulp for 48 hours. Subjects were divided randomly into Group I (negative control), Group II (pulpitis treated by Myrmecodia pendans extract ethanol as treatment group), and Group III (pulpitis treated by Ca(OH)2 as positive control group). Group II and III as pulpitis treatment groups were divided into subgroups based on the induction periods of 48 hours (2 days), 168 hours (7 days), and 366 hours (14 days). All specimens were processed into the slides and evaluated microscopically for the healing process.Results: The result of this study showed significant difference (p<0.05) among groups on day 2, 4 and 7. On day 4, the pulpitis treatment group of Myrmecodia pendans extract showed better healing process than Ca(OH)2. On day 7, the pulpitis treatment group of Ca(OH)2 showed better healing process than Myrmecodia pendans extract. On day 14, both of the pulpitis treatment groups showed normal pulp.Conclusion: Myrmecodia pendans ethanol extract is effective for the healing process of inflamed pulp.Keywords: inflamed pulp, Myrmecodia pendans, sarang semut, Ca(OH)2 , healing process

scholarly journals The efficacy taro leaf extract on wound healing contaminated with Staphylococcus aureus bacteria

2021 ◽  
Vol 33 (3) ◽  
pp. 199
Author(s):  
Endang Sjamsudin ◽  
Annisya Muharty ◽  
Lucky Riawan ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Treatment Group ◽  
Leaf Extract ◽  
Healing Process ◽  
Colocasia Esculenta ◽  
Control Group ◽  
Sprague Dawley ◽  
Group I ◽  
Sprague Dawley Rats

Introduction: taro leaves (Colocasia esculenta (L.) Schott) have been reported to possess wound healing, anti-inflammatory, antibacterial and antioxidant activities. One of the factors that affect wound healing is infection in the wound, wherein the wound is infected by  bacteria, especially Staphylococcus aureus that is mostly found in the oral cavity, the wound healing process will be hampered and become longer healed.The objective of this research was to analyze the efficacy of Colocasia esculenta (L.) Schott extract on the healing process of wounds contaminated with Staphylococcus aureus. Methods: This experimental study on Sprague dawley rats was carried out at the Teaching Veterinary Hospital, IPB . Twenty-four Sprague dawley rats were made with circular excision wounds with a diameter of 2 cm on the dorsum, and Staphylococcus aureus specimens were contaminated with a dose of 3.4x108 LAC. Rats were randomly divided into 2 groups. Group I was given a placebo, group II was given a 25% concentration of Colocasia esculenta (L.) Schott extract on the wound once a day. Four rats from each group were euthanized on days 3, 7, and 14. The number of neutrophils, macrophages, re-epithelialization and FGF-2 expression were measured by microscopic observation with visual field control, and comparative data were analyzed using the Mann-Whitney statistical test. Results: The number of neutrophils on day 7 of the Colocasia esculenta (L.) Schott leaf extract group was less than the control group (p=0.040). The number of macrophages on the 3rd day was more in the treatment group than the control group (p=0.032), and on the 14th day, the treatment group was less than the control group (p=0.040). Epithelial cells on the 14th day of the treatment group were more than the control group (p=.0.017). The level of FGF-2 expression on day 7 of the treatment group was higher than the control group (p=0.044). Conclusion: Application of extracts  Colocasia esculenta (L.) Schott leaf is proven to be efficacious on day 7 for wound healing contaminated with Staphylococcus aureus bacteria.

Effect Of Pregnancy Exercise On Third Trimester Primigravida Anxiety In Dealing With Childbirth

2021 ◽  
pp. 76-83
Author(s):  
Ismi Puji Astuti
Keyword(s):  
Treatment Group ◽  
Sampling Technique ◽  
The Body ◽  
Rank Test ◽  
Control Group ◽  
P Value ◽  
Research Subjects ◽  
Third Trimester ◽  
Quasi Experimental ◽  
Signed Rank Test

Background: The first labor anxiety is an unpleasant psychological condition due to physiological changes that cause psychological instability. The condition of excessive anxiety, worry, fear without cause, and stress, causes the muscles of the body to tense up, especially the muscles in the birth path to become stiff and hard, making it difficult to expand. This study analyzed the effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth. Methods: Quasi experimental design with nonrandomized pretest-postest control group. The sampling technique used was purposive sampling with 34 research subjects. 17 people for the treatment group were given pregnancy exercise 8 times, and 17 people for the control group were given health education about preparation for childbirth. Results: The results of data analysis using the Wilcoxon signed rank test showed that the average reduction in the treatment group was 8.00 and the control group was 3.00. The effect of pregnancy exercise on third trimester primigravida anxiety in the treatment group with a p-value (0.000) <(0.05), while the control group with a p-value (0.180)> (0.05). Conclusion: There is an effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth in the Wuryantoro Health Center work area. 

Sign in / Sign up

Export Citation Format

Share Document