scholarly journals Effect of adding Ferula hermonis Boiss roots and some antioxidants to Tris extender on post-cryopreserved sperm’s cell individual motility and live sperm percentages of Holstein bulls

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
T Abdulkareem ◽  
R Khalil ◽  
A Salman
Keyword(s):  
Vitamin C ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Experimental Period ◽  
Total Phenolic ◽  
Control Group ◽  
Alcoholic Extract ◽  
Poisoning Effect ◽  
Holstein Bulls ◽  
Live Sperm

This study was carried out to explore the adding effect of alcoholic extract of Ferula hermonis Boiss roots and some antioxidants to Tris extender on post-cryopreserved sperm's cell individual motility and live sperm percentages of Holstein bulls for different preservation periods (cooling at 5°C, 2, 30 and 60 days post cryopreservation, PC). Eight Holstein bulls of 2.5-3 years of age were used in this study. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7-week experimental period. Pooled semen was equally divided into eight treatments using Tris extender. The alcoholic extract of Ferula hermonis Boiss roots (0.03 ml/ 50 ml extender; T2), L-Carnitine (0.06g/ 50 ml extender; T3), reduced glutathione (0.03 g/ 50 ml extender; T4), vitamin C (0.2 g/ 50 ml extender; T5), L-Carnitine; 0.06g/ 50 ml extender + alcoholic extract of Ferula hermonis Boiss roots; 0.03 ml/ 50 ml extender (T6), reduced glutathione; 0.03 g/ 50 ml extender + alcoholic extract of Ferula hermonis Boiss roots; 0.03 ml / 50 ml extender (T7) and vitamin C; 0.2 g/ 50 ml extender + alcoholic extract of Ferula hermonis Boiss roots; 0.03 ml/ 50 ml extender (T8) were added to Tris extender and comparisons in response were made with the control group (Tris extender, A1). The total phenolic compound of the extract was 124.38 ± 5.05 mg GAE/ g extract and the extract with 0.01 and 0.03% did not hemolyze the red blood cells and had not poisoning effect on blood cells. The T2 group exhibited higher (P≤0.05) sperm's cells individual motility and live sperm percentage as compared with the T1 group at all preservation periods. On the other hand, T3 group was significantly (P≤0.05) exhibited greater percentages of sperm's cells individual motility and live sperms percentages in comparison with the T1 group at all preservation periods. In conclusion, the adding of alcoholic extract of Ferula hermonis Boiss roots (0.03 ml/ 50 ml extender) and L-Carnitine (0.06g/ 50 ml extender) to Tris extender had an obvious influence in enhancing the sperm's cell individual motility and live sperm percentages of Holstein bulls at different cooling and cryopreservation periods as compared with the control (T1) group. This may contribute to a positive enhancement in conception and pregnancy rates of the inseminated cows, and consequently increase the owner's economic income.

scholarly journals Effect of adding Ferula hermonis Boiss roots and some antioxidants to Tris extender on post-cryopreserved sperm abnormalities percentage of Holstein bulls

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
T bdulkareem ◽  
R Khalil ◽  
A Salman
Keyword(s):  
Vitamin C ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Total Phenolic ◽  
Control Group ◽  
Alcoholic Extract ◽  
Sperm Abnormalities ◽  
Time Period ◽  
Cryopreserved Sperm ◽  
Holstein Bulls

This study was carried out to explore the adding effect of alcoholic extract of Ferula hermonis Boiss roots and some antioxidants to Tris extender on post-cryopreserved sperm abnormalities percentage of Holstein bulls for different preservation periods (cooling at 5°C, 2, 30 and 60 days post cryopreservation, PC). Eight Holstein bulls of 2.5-3 years of age were used in this study. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7-week experimental period. Pooled semen was equally divided into eight treatments using Tris extender. The alcoholic extract of Ferula hermonis Boiss roots (0.03 ml/ 50 ml extender; T2), L-Carnitine (0.06g/ 50 ml extender; T3), reduced glutathione (0.03 g/ 50 ml extender; T4), vitamin C (0.2 g/ 50 ml extender; T5), L-Carnitine; 0.06g/ 50 ml extender + alcoholic extract of Ferula hermonis Boiss roots; 0.03 ml/ 50 ml extender (T6), reduced glutathione; 0.03 g/ 50 ml extender + alcoholic extract of Ferula hermonis Boiss roots; 0.03 ml/ 50 ml extender (T7) and vitamin C; 0.2 g/ 50 ml extender + alcoholic extract of Ferula hermonis Boiss roots; 0.03 ml/ 50 ml extender (T8) were added to Tris extender and comparisons in response were made with the control group (Tris extender, A1). The total phenolic compound of the extract was 124.38 ± 5.05 mg GAE/ g extract and the extract with 0.01 and 0.03% did not hemolyze the red blood cells and had not poisoning effect on blood cells. The T2 group exhibited lesser (P≤ 0.01) percentage of sperm tail midpiece abnormalities as compared with the T1 group at all preservation periods. Moreover, the T3 was also recorded lesser (P≤0.01) sperm's head abnormalities percentage at 60 days PC time period in comparison with the T1 group. On the other hand, T4, T5, T6, T7 and T8 exhibited lesser (P≤0.05) total sperm abnormalities percentage at 60 days PC time period as compared with the T1 group. In conclusion, the adding of alcoholic extract of Ferula hermonis Boiss roots (0.03 ml/ 50 ml extender) and L-Carnitine (0.06g/ 50 ml extender) to Tris extender had an obvious influence in reducing the sperm abnormalities percentage of Holstein bulls at different cooling and cryopreservation periods as compared with the control (T1) group. This may contribute to a positive enhancement in conception and pregnancy rates of the inseminated cows, and consequently increase the owner's economic income.

scholarly journals Effect of adding aqueous extract of Melissa officinalis leaves and some other antioxidants to milk–based extender on post-cooling and post- cryopreservative sperm’s individual motility and live sperm percentage of Holstein bulls

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
T Abdulkareem ◽  
O Alzaidi
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Aqueous Extract ◽  
Experimental Period ◽  
The Other ◽  
Melissa Officinalis ◽  
Holstein Bulls ◽  
Time Periods ◽  
Animal Resource ◽  
Live Sperm

This study was conducted to explore the effect of adding aqueous extract of Melissa officinalis leaves (AEMOL), some antioxidants and their combinations to the milk-based extenderfor increase on sperm's cells individual motility percentage on and live sperms percentage semen for Holstein bulls following different preservation periods. The study was executed at the Department of Artificial Insemination, Abu-Ghraib belong to the Directorate of Animal Resource, Ministry of Agriculture, during the period from October 2015 to February 2016 using ten Holstein bulls of 2.5-3 years old. Semen was collected via artificial vagina in one ejaculate per bull per week for the 7-week experimental period. Pooled semen was equally divided into ten groups within one experiment. In this experiment, AEMOL (0.062mg/100 ml; A2), WEMOL (0.031 mM) + 5 mM of vitamin C (A3), AEMOL (0.062mg/100 ml + 0.08 mM of Trolox (A4),AEMOL (0.062mg/100 ml + 100 IU of catalase (A5),100 IU of catalase (A6), 5 mM of vitamin C (A7), 0.08 mM of Trolox (A8), 0.2 mM of vitamin E (A9) and AEMOL (0.062mg/100 ml +0.2 mM of vitamin E (A10). The effect of these additives on semen characteristics of Holstein bulls for different preservation periods (cooling at 5◦C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC) were studied. The A2 group exhibited greater (P≤ 0.01) sperm's cell individual motility, live sperm percentage, as compared with A1 group at all preservation periods. Greater sperm's cells individual motility, live sperm percentage noticed in A4 group in comparison with the A1 group at all preservation time periods. On the other hand, A5 group exhibited greater (P≤ 0.01) sperm's cell individual motility, live sperm percentage, as well as, adding of vitamin E alone (A9) or combined with AEMOL (A10) to milk-based extender had a positive (P≤ 0.01) effect in improving sperm's cell individual motility and live sperm percentage at all preservation time periods. In conclusion, adding of AEMOL as alone or combined with the other synthetic antioxidants to milk-based extenders had a crucial role in improving PC sperm's cell individual motility and live sperm percentage of Holstein bulls. This was reflected positively on increasing pregnancy rates of the inseminated cows.

scholarly journals Cardiovascular and hematologic effects produced by chronic treatment with etoricoxib in normotensive rats

2009 ◽  
Vol 24 (3) ◽  
pp. 206-210 ◽  
Author(s):  
Nilo César do Vale Baracho ◽  
Guilherme Pedrosa Guizelli ◽  
Beatriz Leone Carmello ◽  
Danielle de Souza Sanches ◽  
Felipe Moraes Costa Silva ◽  
...  
Keyword(s):  
Blood Cells ◽  
Chronic Treatment ◽  
Experimental Period ◽  
Control Group ◽  
Histopathological Changes ◽  
Study Results ◽  
Significant Difference ◽  
Hematological Effects ◽  
The Mean ◽  
Hematological Changes

PURPOSE: Evaluate the cardiovascular and hematological effects produced by chronic treatment with two dosis of etoricoxib in Wistar normotensive rats. METHODS: Thirty rats have been used and divided into one control group and two etoricoxib (10mg/kg and 30mg/kg) treatments groups for 60 days. The mean arterial pressure (MAP) was taken during the whole experimental period and at the end of this period, under anesthesia blood samples were taken, and further the withdrawn of the aorta, heart, brain, liver, and kidneys for the anatomopathologic study. RESULTS: The treatment with etoricoxib (30mg/Kg) produced a significant increase of the MAP from the 28th day of the experiment and from the platelets when compared to the control group and to the group treated with 10mg/Kg, besides producing a highly significant difference in hematocrit and in the red blood cells in relation to the control group. On the other hand the treatment with etoricoxib has not caused histopathological changes when compared to the control. CONCLUSION: These data show that the chronic treatment with etoricoxib leads to increase of the MAP, and to important hematological changes which seem to be associated to the hemoconcentration although not producing anatomopathological significant changes.

scholarly journals Antioxidant effect on viability of boar semen cooled to 15°C

2020 ◽  
Vol 72 (1) ◽  
pp. 145-152
Author(s):  
V.E.D. Martins ◽  
S.C.C. Pinto ◽  
R.M. Chaves ◽  
A.K.D. Barros Filho ◽  
L.M. Laskoski ◽  
...  
Keyword(s):  
Vitamin C ◽  
Sperm Motility ◽  
Reduced Glutathione ◽  
Antioxidant Effect ◽  
Control Group ◽  
Promising Alternative ◽  
Sperm Characteristics ◽  
Boar Semen

ABSTRACT This study aimed to evaluate the addition of Vitamin C, reduced Glutathione and trolox on sperm characteristics of pork refrigerated semen. Six pigs were collected through the technique of gloved hand (10 ejaculates/animals). The semen was diluted in MR-A®. After the previous evaluations, the treatments were added: Control group: diluent only; Vitamin C Group: 200μM/mL Vitamin C; Trolox Group: 200μM/mL Trolox; Glutathione group: 2.5mM/ml Reduced glutathione. The semen was stored in thermal boxes and placed inside the refrigerator at 15oC and evaluated at D0, 12, 48, 72 hours. After 30 hours of incubation, each treatment was divided into two equal fractions and the same concentration of antioxidants was added in one of the parts. The results show that reduced glutathione supplementation preserves sperm motility after 24 hours but also has a higher percentage of acrosome intact in the presence of this antioxidant. There was no effect of adding a second dose of the antioxidants. In conclusion, the addition of reduced Glutathione to the swine semen diluent is a promising alternative for better preservation of sperm characteristics and the addition of the second dose of antioxidants during storage is detrimental to semen.

scholarly journals Protective Effects of Myrtus communis Linn Fruit and Leaf Extracts on Isoproterenol-Induced Heart Failure in Rat

2021 ◽  
Author(s):  
Nahid Aboutaleb ◽  
Fataneh Hashem-Dabaghian ◽  
Azinmehr Elahian Boroujeni ◽  
Asie Shojaii
Keyword(s):  
Oxidative Stress ◽  
Heart Failure ◽  
Cardiac Fibrosis ◽  
Experimental Period ◽  
Stress Factors ◽  
Control Group ◽  
Alcoholic Extract ◽  
Protective Effects ◽  
Myrtus Communis ◽  
Leaf Extracts

Myrtus communis Linn. (MC) is a cardiotonic plant in traditional Persian medicine. This study was conducted to evaluate the protective effect of MC on isoproterenol-induced heart failure (HF) in rats. Isoproterenol was injected subcutaneously in all groups except the control group for 4 consecutive days to induce myocardial injury in male Wistar rats. In the case of treatment groups, the animals were treated with different doses of the hydro-alcoholic extract of MC fruit or leaves (150, 300, and 700 mg/kg), and were compared with healthy and HF rats. In order to evaluate cardiac function, echocardiography was performed on day 28 after treatment. MC fruit and leaf extracts were administered to all groups except the healthy control group for 28 days by gavage. At the end of the experimental period, the animals were sacrificed and the left ventricle regions of tissue hearts were collected to measure the levels of oxidative stress factors (MDA, SOD, GSH) using ELISA methods. Cardiac fibrosis was evaluated by Mason’s trichrome staining. Hematoxylin-eosin staining was used to assess histopathological changes in cardiac structure.  Our results showed that administration of MC fruit and leaf extracts significantly reduced the MDA level and increased SOD and GSH levels in treated HF rats compared to the HF group (P<0.05). In addition, MC mitigated fibrosis and improved cardiac histological changes compared to the HF group. Collectively, our findings show that MC can be considered as a good candidate to provide cardioprotective effects in HF rats through reduction of oxidative stress and myocardial fibrosis.

scholarly journals The variation in the reduced glutathione levels of red blood cells between full- term Libyan newborns with hyperbilirubinemia

2020 ◽  
pp. 135-141
Author(s):  
Nura Nasib Mohamed ◽  
Mustafa Ziu ◽  
Nammah Elhoni ◽  
Mohamed Alkhadar
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Full Term ◽  
Total Serum ◽  
Control Group ◽  
Blood Exchange ◽  
Yellow Coloration ◽  
The Central Nervous System ◽  
Term Newborns

Background: Hyperbilirubinemia refers to the yellow coloration of the skin and the sclera of newborn babies that result from the accumulation of bilirubin in the skin and mucous membranes. Newborns with jaundice need to be monitored because bilirubin is potentially toxic to the central nervous system. Aim of the study: To determine the relation of erythrocytic glutathione with hyperbilirubinemia in newborns. Methods: We studied 45 Libyan full-term newborns (23 male and 22 female) with neonatal hyperbilirubinemia aged from day one to day four born in Al- Jomhoriya hospital in Benghazi. We excluded each case with pathological jaundice that needs treatment by phototherapy or blood exchange. All investigations concerning this research carried out on each newborn every 24 hours in a period of four days as follow: day 1 up to day 4, day 2 up to day 5, day 3 up to day 6, day 4 up to day 7. We analyzed the total serum bilirubin (TSB) and the level of reduced glutathione (GSH) in red blood cells directly on fresh blood. Conclusion: Our results show no significant variations in the mean level of erythrocytic glutathione in the jaundiced full-term newborns compared to the control group in the first week of life.

scholarly journals In Vivo Screening and Antidiabetic Potential of Polyphenol Extracts from Guava Pulp, Seeds and Leaves

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1714
Author(s):  
Hassan Shabbir ◽  
Tusneem Kausar ◽  
Sobia Noreen ◽  
Hafeez ur Rehman ◽  
Ashiq Hussain ◽  
...  
Keyword(s):  
Feed Intake ◽  
Blood Cells ◽  
White Blood Cells ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Total Phenolic ◽  
Control Group ◽  
Albino Rats ◽  
Control Groups

The present study investigates the antidiabetic potential of polyphenol extracts purified from guava pulp, seeds and leaves using an in vivo experiment on albino rats. The polyphenols from guava pulp, seeds and leaves were extracted using methanol solvent and the sonication method while being evaluated by total phenolic contents and radical scavenging activity assay. The proximate composition of powders revealed that ash, protein and total sugars were significantly (p < 0.05) higher in leaves and seeds, while vitamin C was highest in pulp. Total phenolic and antioxidant activities were highest in pulp followed by leaves and seeds. The findings of feed intake and body gain revealed that the supplementation of polyphenols, especially from pulp, significantly (p < 0.05) increased the feed intake, which resulted in increased body weight. Moreover, total cholesterol (TC) and low-density lipoprotein (LDL) levels were significantly (p < 0.05) decreased, while the level of high-density lipoprotein (HDL) was increased in groups fed with polyphenols from guava pulp compared to both (+ive and –ive) control groups. Furthermore, blood glucose and triglycerides were significantly (p < 0.05) decreased in supplemented groups compared to the control group of diabetes mice, which resulted in the inhibition of α-amylase and glucose transport. Besides this, packed cell volume (PCV), mean corpuscular volume (MCV), hemoglobin, red blood cells (RBCs), white blood cells (WBCs) and platelet levels were increased significantly (p < 0.05) in pulp’s extract followed by leaves and seeds compared to both control groups. Overall, the antidiabetic potential of different extracts was in the following order: pulp > leaves > seeds. The findings suggest the feasibility of adding 200–250 mg/kg.bw of polyphenol extracts of pulp as an alternative to diabetic drugs.

scholarly journals EFFECT OF ADDING PENTOXIFYLLINE AND NITRIC OXIDE TO TRIS EXTENDER ON SOME POST-CRYOPRESERVED SEMEN ATTRIBUTES OF HOLSTEIN BULLS

2020 ◽  
Vol 51 (2) ◽  
pp. 619-628
Author(s):  
Mohammed & et al.
Keyword(s):  
Nitric Oxide ◽  
Plasma Membrane ◽  
Membrane Integrity ◽  
Control Group ◽  
Plasma Membrane Integrity ◽  
Abnormal Sperm ◽  
Semen Characteristics ◽  
Holstein Bulls ◽  
Time Periods ◽  
Live Sperm

This study was aimed to explore the effect of adding pentoxifylline (PTX) and nitric oxide (NO) to Tris extender on some post-cryopreserved semen attributes of Holstein bulls for different preservation periods (cooling at 5◦C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC). Seven Holstein bulls of 2.5-3 years old were used in the current study during the period from 20th November, 2017 to 20th August, 2018. Pooled semen was equally divided into three groups within one experiment. PTX (0.192 g / 100 ml extender) and NO (0.018 g / 100 ml extender) were added to Tris extender and comparisons in response were made with the control group (Tris extender, C). The PTX group exhibited greater (P≤ 0.01) sperm's cell individual motility percentage as compared with the C group at cooling (5ºc) and 48 hr PC periods, while, PTX and NO groups were superior in these percentages at the remaining PC periods than C group. Excluding data of 2 months PC, greater (P≤ 0.01) live sperm percentage was observed in PTX and NO groups in comparison with the C group at all preservation periods. Lesser (P≤ 0.01) abnormal sperm percentage were noticed for PTX and NO groups as compared with the C group at all preservation time periods. The PTX and NO groups exhibited greater   (P≤ 0.01) acrosome and plasma membrane integrity percentages in comparison with the C group at all preservation time periods. In conclusion, adding PTX and NO to Tris extender enhanced some of  PC semen characteristics of Holstein bulls at different preservation periods.

scholarly journals Study on the Pharmacological Character of an Insulin-Mimetic Small Molecular Compound of Vanadyl Trehalose

2020 ◽  
pp. 481-490
Author(s):  
M UMAR ◽  
W QIAN ◽  
Q LIU ◽  
S XING ◽  
X LI ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastrointestinal Tract ◽  
Vitamin C ◽  
Reduced Glutathione ◽  
Control Group ◽  
Glutathione Synthetase ◽  
Molecular Compound ◽  
Glutathione S Transferase ◽  
Pathway Gene ◽  
Group A

To investigate the effect of vanadyl trehalose (VT) on oxidative stress and reduced glutathione/glutathione-S-transferase (GSH/GSTs) pathway gene expression in mouse gastrointestinal tract, as well as the protective effects of vitamin C (VC) and reduced glutathione (GSH). Thirty male Kunming mice were randomly divided into five groups: control group (group A), VT group (group B), VC + VT group (group C), GSH + VT group (group D) and VC + GSH + VT group (group E). The content of reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) activity and the expressions of glutamate-cysteine ligase catalytic subunit (GCLC), glutathione synthetase (GSS), regulated through glutathione reductase (GSR) and glutathione-S-transferase pi (GSTpi) in stomach and duodenum in vanadyl trehalose treated group were lower than those in group A (P<0.05). The C, D, E group can significantly improve the above indicators, but those only in the stomach in E group reached the level of the control group. Vanadyl trehalose (VT) was able to cause oxidative stress damage to the gastrointestinal tract of mice, which affects GSH content and GSH-Px activity and interferes with the normal expression of GSH/GSTs pathway. Exogenous vitamin C, reduced glutathione and the combination of the two could play a specific role in antioxidant protection and reduce the toxicity of vanadyl trehalose.

scholarly journals The use of vitamin "C" as antioxidant to decrease lesions of atherosclerosis that induced experimentally by hydrogen peroxide in rabbits

2005 ◽  
Vol 29 (1) ◽  
pp. 51-63
Author(s):  
D. S. Diab
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Vitamin C ◽  
Lipid Profile ◽  
Experimental Period ◽  
Control Group ◽  
Plasma Samples ◽  
Blood Samples ◽  
Atherosclerotic Lesions ◽  
Stress Changes

This study was designed to investigate the effect of vitamin C inalleviating the changes in lipid profile and the atherosclerotic lesions inducedexperimentally by (0.5%) H2O2 in drinking water. Twenty four adult malesrabbits were divided randomly into four equal (a control and 3 treated "T")groups for 19 weeks: 1- The Control group: Which was given normal drinkingwater. 2- Group (T1): was given 0.5% H 2O2 in drinking water. 3- Group(T2): which was handled as T1, in addition to vitamin C (100 mg /kg/day) alongthe experiment. 4- Group (T3): was treated as T1 till the atherosclerotic lesionswere established at the week 13, then the animals received 100 mg/kg of vitaminC daily for 6 weeks. Blood samples were taken at the weeks 5, 13, 16 and 19 ofthe experimental period to measure the levels of total cholesterol (TC),triglycerid (TG), HDL-C, LDL-C and VLDL-C in plasma. Samples of aortictissue were taken to study the histopatholpgical changes. The results showed thatH2O2 caused an increase in TC, TG, LDL, VLDL-C and a decrease in HDL-Cconcentration in plasma. The histopathological sections showed lesions ofatherosclerosis in the aorta as fatty streaks and foam cells. Moreover, resultsshowed that treatment with vitamin C caused a dereased in the VLDL-C, LDLC,TG and TC levels and an increase in the HDL-C concentration in plasma. andregression of atherosclerotic lesions. Results of the present study suggest that theoxidative stress (produced by 0.5% H2O2) has an important role in pathogenesisof atherosclerosis in the rabbits and vitamin C has an important effect inreducing and preventing some of the oxidative stress changes in lipid profile andatherosclerotic lesions of the aorta. It is worthed to report that this is the firststudy undertaken in using vitamin C as an antioxidant to minimize the oxidativestress effects of H2O2 in rabbits as a model for atherosclerosis in mammals.

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