scholarly journals Protective Potential of Ginseng and/or Coenzyme Q10 on Doxorubicin-induced Testicular and Hepatic Toxicity in Rats

2021 ◽  
Vol 9 (A) ◽  
pp. 993-1005
Author(s):  
Suzan Khodir ◽  
Aliaa Alafify ◽  
Essam Omar ◽  
Marwa Al-Gholam
Keyword(s):  
Gene Expression ◽  
Serum Cholesterol ◽  
Coenzyme Q10 ◽  
Interleukin 6 ◽  
Liver Enzymes ◽  
Serum Testosterone ◽  
Interleukin 10 ◽  
Therapeutic Effects ◽  
Hepatic Toxicity ◽  
Tnf Α

Introduction: Although doxorubicin (DOX) is a successful cancer chemotherapeutic, side effects limit the clinical utility of DOX-based therapy, including male infertility and hepatotoxicity. Objective: To evaluate the testicular and hepatoprotective effect of ginseng and/or coenzyme Q10 (CoQ10) in rats exposed to DOX and the possible underlying mechanisms. Materials and Methods:  Fifty adult male albino rats were divided into (10/group), control, DOX group, DOX/Gin group, DOX/CoQ10 group and DOX/Gin+CoQ10 group. Serum testosterone, serum liver enzymes, fasting serum cholesterol and triglyceride (TG), tissue malondialdehyde (MDA), tissue superoxide dismutase (SOD), serum tumor necrosis factor-alpha (TNF-α), serum interleukin 6, serum interleukin 10, nuclear factor E2‐related factor 2 (Nrf2) gene expression in liver and testis and organ indices were measured.  Histopathological and immunohistochemical assessments of apoptotic marker kaspase3 in testis and liver were also performed. Results DOX-induced toxicity is associated with a significant decrease in serum testosterone, testis and liver index values, testicular and hepatic SOD,  testicular and hepatic Nrf2 gene expression and serum interleukin 10. However, there was a significant increase in serum liver enzymes, serum cholesterol and TG, testicular and hepatic MDA, serum TNF-α and serum interleukin 6 when compared with the control group. The combination of ginseng and CoQ10 resulted in significant improvement of DOX-induced changes when compared with other treated groups. Conclusion: Ginseng and CoQ10 have valuable therapeutic effects on DOX-induced testicular and hepatic toxicity via up-regulation of Nrf2 gene expression, inhibition of apoptosis, anti-oxidant, anti-inflammatory and hypolipidemic effects.

Copper decreases gene expression of TNF-α, IL-10, and of matrix metalloproteinases MMP-2 and MMP-9 in isolated perfused rat livers

Biologia ◽  
2007 ◽  
Vol 62 (3) ◽  
Author(s):  
Albena Alexandrova ◽  
Elena Bandžuchová ◽  
Anton Kebis ◽  
Marián Kukan ◽  
Daniel Kuba
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Matrix Metalloproteinase ◽  
Oxidative Dna Damage ◽  
Interleukin 10 ◽  
Matrix Metalloproteinase 2 ◽  
Necrosis Factor Alpha ◽  
Tissue Samples ◽  
Tnf Α ◽  
Rat Livers

AbstractCopper is known to induce oxidative stress in a number of models. It was shown that many pathophysiological events were associated with oxidative stress. Further, oxidative stress can increase gene expression of cytokines and of metalloproteinases. We previously found that copper toxic effects in isolated perfused rat livers were associated with significant oxidative stress (as assessed by lipid peroxidation, protein oxidation and oxidative DNA damage, particularly at concentration of 0.03 mM of Cu2+ in the perfusate). Here we investigated gene expression of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10); matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in frozen liver tissue samples by the real-time PCR assay. Compared to controls, copper at concentration of 0.01 mM did not affect gene expression of TNF-α, IL-10, MMP-2 and MMP-9, whereas copper at concentration of 0.03 mM significantly decreased gene expression of all the four TNF-α, IL-10, MMP-2 and MMP-9 by 69%, 81%, 43%, and 62%, respectively. These results suggest that copper-induced oxidative stress in the isolated rat liver can lead to the suppression of gene expression. Because TNF-α and metalloproteinases are involved also in liver regeneration, the suppression of these genes by copper may be one of the mechanisms by which acute intoxication of animals and humans with copper may impair regenerative capability of the liver.

scholarly journals Maternal Expressions (Serum Levels) of Alpha Tumour Necrosis Factor, Interleukin 10, Interleukin 6 and Interleukin 4 in Malaria Infected Pregnant Women Based on Parity in a Tertiary Hospital in Southeast, Nigeria

2020 ◽  
pp. 35-41
Author(s):  
Obeagu, Emmanuel Ifeanyi ◽  
Obeagu, Getrude Uzoma ◽  
Amaeze, Augustine Amaeze ◽  
Asogwa, Eucharia Ijego ◽  
Chukwurah, Ejike Felix ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Tumour Necrosis Factor ◽  
Interleukin 6 ◽  
Tumour Necrosis ◽  
Public Health Problem ◽  
Serum Levels ◽  
Interleukin 10 ◽  
Interleukin 4 ◽  
Tnf Α ◽  
Necrosis Factor

Malaria has been reported as a condition caused by infestation with Plasmodium parasite specie, which is a great public health problem globally, particularly in developing countries like Nigeria. This study was carried out in Federal Medical Centre Umuahia in Abia State, Nigeria. The study was done to determine the maternal serum levels of alpha tumour necrosis factor, interleukin 10, interleukin 6, and interleukin 4 in malaria-infected pregnant women based on parities in Southeast, Nigeria.  A total of 150 subjects between the ages of 18-45 years were recruited for the study comprising 50 subjects each of 3 parities (groups A-C). A commercial ELISA Kit was used to measure all the cytokines. Neither statistically significant differences were found for TNF-α (p=0.636), IL-10 (p=0.892), IL-6 (p=0.306) and IL-4 (p=0.222) between prime parity and second parity nor for TNF-α (p=0.356), IL-10 (p=0.896), IL-6 (p=0.304) and IL-4 (p=0.298) between prime parity and multi-parity of malaria-infected pregnant women. TNF-α (p=0.255), IL-10 (p=0.524), IL-6 (p=0.616), and IL-4 (p=0.672) between second parity and on multi-parity respectively. The study showed no changes in the cytokines studied among the malaria-infected pregnant women based on parities. It shows that the number of pregnancies in women infected with malaria has no changes in the levels of the cytokines studied.

scholarly journals Transplantation of bone marrow derived macrophages reduces markers of neuropathology in an APP/PS1 mouse model

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Luís Costa-Marques ◽  
Katrin Arnold ◽  
Marie-Christine Pardon ◽  
Christiane Leovsky ◽  
Samantha Swarbrick ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Cell Therapy ◽  
Transgenic Mice ◽  
Amyloid Beta ◽  
Exploratory Study ◽  
Interleukin 10 ◽  
Mechanisms Of Action ◽  
Prostaglandin E ◽  
Therapeutic Effects

Abstract Background We investigated early hallmarks of putative therapeutic effects following systemic transplantation of bone marrow derived macrophages (BM-M) in APP/PS1 transgenic mice. Method BM-M were transplanted into the tail vein and the animals analysed 1 month later. Results BM-M transplantation promoted the reduction of the amyloid beta [37-42] plaque number and size in the cortex and hippocampus of the treated mice, but no change in the more heavily modified pyroglutamate amyloid beta E3 plaques. The number of phenotypically ‘small’ microglia increased in the hippocampus. Astrocyte size decreased overall, indicating a reduction of activated astrocytes. Gene expression of interleukin 6 and 10, interferon-gamma, and prostaglandin E receptor 2 was significantly lower in the hippocampus, while interleukin 10 expression was elevated in the cortex of the treated mice. Conclusions BM-M systemically transplanted, promote a decrease in neuroinflammation and a limited reversion of amyloid pathology. This exploratory study may support the potential of BM-M or microglia-like cell therapy and further illuminates the mechanisms of action associated with such transplants.

scholarly journals Interleukin-10 attenuates TNF-α–induced interleukin-6 production in endometriotic stromal cells

2009 ◽  
Vol 91 (5) ◽  
pp. 2185-2192 ◽  
Author(s):  
Yukiko Tagashira ◽  
Fuminori Taniguchi ◽  
Tasuku Harada ◽  
Ayako Ikeda ◽  
Ayako Watanabe ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Interleukin 6 ◽  
Interleukin 10 ◽  
Tnf Α

scholarly journals Antisense Tissue Factor Oligodeoxynucleotides Protected Diethyl Nitrosamine/Carbon Tetrachloride-Induced Liver Fibrosis Through Toll Like Receptor4-Tissue Factor-Protease Activated Receptor1 Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Maha M. Shouman ◽  
Rania M. Abdelsalam ◽  
Mahmoud M. Tawfick ◽  
Sanaa A. Kenawy ◽  
Mona M. El-Naa
Keyword(s):  
Gene Expression ◽  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Tissue Factor ◽  
Transforming Growth Factor ◽  
Liver Enzymes ◽  
Smooth Muscle Actin ◽  
Coagulation Factor ◽  
Enzymes Activities ◽  
Tnf Α

Tissue factor (TF) is a blood coagulation factor that has several roles in many non-coagulant pathways involved in different pathological conditions such as angiogenesis, inflammation and fibrogenesis. Coagulation and inflammation are crosslinked with liver fibrosis where protease-activated receptor1 (PAR1) and toll-like receptor4 (TLR4) play a key role. Antisense oligodeoxynucleotides are strong modulators of gene expression. In the present study, antisense TF oligodeoxynucleotides (TFAS) was evaluated in treating liver fibrosis via suppression of TF gene expression. Liver fibrosis was induced in rats by a single administration of N-diethyl nitrosamine (DEN, 200 mg/kg; i. p.) followed by carbon tetrachloride (CCl4, 3 ml/kg; s. c.) once weekly for 6 weeks. Following fibrosis induction, liver TF expression was significantly upregulated along with liver enzymes activities and liver histopathological deterioration. Alpha smooth muscle actin (α-SMA) and transforming growth factor-1beta (TGF-1β) expression, tumor necrosis factor-alpha (TNF-α) and hydroxyproline content and collagen deposition were significantly elevated in the liver. Blocking of TF expression by TFAS injection (2.8 mg/kg; s. c.) once weekly for 6 weeks significantly restored liver enzymes activities and improved histopathological features along with decreasing the elevated α-SMA, TGF-1β, TNF-α, hydroxyproline and collagen. Moreover, TFAS decreased the expression of both PAR1 and TLR4 that were induced by liver fibrosis. In conclusion, we reported that blockage of TF expression by TFAS improved inflammatory and fibrotic changes associated with CCl4+DEN intoxication. In addition, we explored the potential crosslink between the TF, PAR1 and TLR4 in liver fibrogenesis. These findings offer a platform on which recovery from liver fibrosis could be mediated through targeting TF expression.

Synthesis and In Vitro Anti-inflammatory Activity of C20 Epimeric Ocotillol-Type Triterpenes and Protopanaxadiol

Planta Medica ◽  
2018 ◽  
Vol 85 (04) ◽  
pp. 292-301 ◽  
Author(s):  
Jianqiang Zhang ◽  
Qian Zhang ◽  
Yangrong Xu ◽  
Huixiang Li ◽  
Fenglan Zhao ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Interleukin 6 ◽  
Inflammatory Mediator ◽  
Interleukin 10 ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Tnf Α ◽  
Anti Inflammatory

AbstractGinseng is a perennial herb that contains various medicinal substances. The major active constituents of ginseng are ginsenosides, which have multifarious biological activities. Some pharmacological activities are closely dependent on the stereoisomers derived from the configuration at C20. In this study, the in vitro anti-inflammatory activity of C20 epimeric ocotillol-type triterpenes (2, 3, 9, and 10) and protopanaxadiol [20(S/R)-protopanaxadiol] were investigated. Epimers 2 and 3 were prepared starting from 20(S)-protopanaxadiol. Epimers 9 and 10 were synthesized from 20(R)-3-acetylprotopanaxadiol (7). The anti-inflammatory activity of 2, 3, 9, 10, 20(S)-protopanaxadiol, and 20(R)-protopanaxadiol was evaluated in cultured mouse macrophage RAW 264.7 cells. The MTT assay was used to measure the cytotoxicity. RAW 264.7 cells were stimulated by lipopolysaccharide to release the inflammatory mediators nitric oxide, prostaglandin E2, TNF-α, and interleukin-6 and anti-inflammatory mediator interleukin-10. The effect of the compounds on the overproduction of nitric oxide, prostaglandin E2, TNF-α, interleukin-6, and interleukin-10 was determined using Griess and ELISA methods. The results demonstrated that the in vitro anti-inflammatory activities of C20 epimeric ocotillol-type triterpenes and protopanaxadiol were different. Both the 20S-epimers (2 and 3) and 20R-epimers (9 and 10) inhibited the release of inflammatory mediator nitric oxide, while mainly the 20S-epimers inhibited the release of inflammatory mediator prostaglandin E2, and the 20R-epimers inhibited the release of inflammatory cytokine TNF-α. Both the 20S-epimers [2, 3, and 20(S)-protopanaxadiol] and 20R-epimers [9, 10, and 20(R)-protopanaxadiol] inhibited the release of inflammatory cytokine interleukin-6, but mainly the 20S-epimers [2, 3, and 20(S)-protopanaxadiol] increased the release of anti-inflammatory mediator interleukin-10.

scholarly journals PSIV-10 Effect of a yeast product supplementation on growth performance, nutrient digestibility and cytokines mRNA expression in weanling piglets reared under low sanitary environment

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 180-180
Author(s):  
Fernando Bravo de Laguna Ortega ◽  
Bruno Bertaud ◽  
In Ho Kim ◽  
Yong Min Kim
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Interleukin 1 ◽  
Test Procedure ◽  
Interleukin 10 ◽  
Nutrient Digestibility ◽  
Interleukin 12 ◽  
Tnf Α ◽  
Weanling Piglets ◽  
Yeast Product

Abstract The objective of the trial was to evaluate the effect of a novel multi-strains yeast fractions product (MsYF) on performance, digestibility, and gene expression of several cytokines in weanling piglets reared under low sanitary environment (uncleaned). In total, 160 piglets weaned at 24 days (7.21 ± 1.05kg) were distributed in 2 treatments according to body weight and sex: control (CON), and MsYF (CON+2kg/ton of yeast product in the first 14 days post weaning; CON+400g/ton of product between days 14 and 42). Individual body weight (BW) and feed intake (FI) were measured on days 1, 14 and 42. Apparent total tract digestibility (ATTD) of dry matter (DM) and crude protein (CP) was estimated at the end of the experiment using chromium oxide as indigestible marker. On days 7, 14 and 42, 6 piglets per treatment were slaughtered and sampled from the mid-jejunum to measure cytokines mRNA gene expression: tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), toll-like receptor 2 (TLR2), interleukin 1 receptor 1 (IL-1-R1), interleukin 10 (IL-10), interleukin 1β (IL-1β), interleukin 12 (IL-12), interferon gamma (INF- γ), and (GATA3). Data were analyzed by ANOVA using the T-test procedure of SAS (SAS Inst. Inc., Cary, US) with treatment as main effect. Piglets fed MsYF had greater (P < 0.05) final BW and greater (P < 0.01) ADG compared to piglets fed CON. Piglets fed MsYF tended to have greater (P < 0.1) ATTD of DM than piglets fed CON. Supplementation of MsYF down-regulated the expressions of TNF-α, IL-6, and TLR2 on days 7 and 14 (P < 0.01 and P < 0.1 for TNF-α, P < 0.01 and P < 0.001 for IL-6, and P < 0.0001 and P < 0.001 for TLR2) while a reduced (P < 0.01) INF-γ expression occurred in piglets fed MsYF at day 14. In conclusion, supplementation with yeast improved performance, DM digestibility, and may modulate intestinal mucosa inflammation of weanling piglets under low sanitary environment.

scholarly journals Effects of Cinnamon extract on biochemical enzymes, TNF-α and NF-κB gene expression levels in liver of broiler chickens inoculated with Escherichia coli

2015 ◽  
Vol 35 (9) ◽  
pp. 781-787 ◽  
Author(s):  
Seyed Mahmoud Tabatabaei ◽  
Reza Badalzadeh ◽  
Gholam-Reza Mohammadnezhad ◽  
Reza Balaei
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Broiler Chickens ◽  
Liver Enzymes ◽  
Gene Expressions ◽  
Expression Levels ◽  
E Coli ◽  
Cinnamon Extract ◽  
Tnf Α ◽  
Gene Expression Levels

Abstract: Infection with Escherichia coli (E. coli) is a common disease in poultry industry. The use of antibiotics to treat diseases is facing serious criticism and concerns. The medicinal plants may be effective alternatives because of their multiplex activities. The aim of this study was to investigate the effects of cinnamon extract on the levels of liver enzymes, tumor necrosis factor-alpha (TNF-α) and nuclear factor-kappa B (NF-κB) gene expressions in liver of broiler chickens infected with E. coli. Ninety Ross-308 broilers were divided into healthy or E. coli-infected groups, receiving normal or cinnamon extract (in concentrations of 100 or 200mg/kg of food) supplemented diets. E. coli suspension (108cfu) was injected subcutaneously after 12 days cinnamon administration. Seventy-two hours after E. coli injection, the blood samples were taken for biochemical analysis of liver enzymes in serum (spectrophotometrically), and liver tissue samples were obtained for detection of gene expression of inflammatory markers TNF-α and NF-κB, using real-time PCR. Infection with E. coli significantly increased the levels of TNF-α and NF-κB gene expressions as well as some liver enzymes including creatine-kinase (CK), lactate-dehydrogenase (LDH), alanine-transferase (ALT) and aspartate-transferase (AST) as compared with control group (P<0.05). Pre-administration of cinnamon extract in broilers diet (in both concentrations) significantly reduced the tissue levels of TNF-α and NF-κB gene expressions and enzymes CK and ALT in serum of broiler chickens inoculated with E. coli in comparison with E. coli group (P<0.05 and P<0.01). The levels of LDH and AST were significantly decreased only by 200mg/kg cinnamon extract in infected broilers. The level of alkaline-phosphatase (ALP) was not affected in any groups. Pre-administration of cinnamon extract in diets of broiler chickens inoculated with E. coli could significantly reduce the gene expression levels of pro-inflammatory mediators and liver enzymes activities, thereby protecting the liver against this pathologic condition.

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