scholarly journals Antimicrobial activity in cultures of endophytic fungi isolated from Talinum triangulare

2021 ◽  
Vol 43 ◽  
pp. e49786
Author(s):  
Claudio Baltazar de Sousa ◽  
Erika Alves Fonseca Amorim ◽  
Rita de Cassia Mendonça Miranda
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Antimicrobial Substances ◽  
Diffusion Technique ◽  
Potential Sources ◽  
Trichoderma Fungi ◽  
Potential Applications ◽  
Corynebacterium Diphtheria ◽  
Talinum Triangulare

. Endophytic fungi colonize the interior of plants without causing damage and act in symbiosis with their host. They are also potential sources of compounds with potential applications in industry, agriculture, and medicine, Thus, this study aimed to isolate and identify the endophytic fungi medicinal plant Talinum triangulare and evaluate its potential for the production of antimicrobial substances using the disk diffusion technique and testing in liquid medium front of Staphylococcus aureus ATCC SA 6538, Pseudomonas aeruginosa ATCC PA 0030, and Corynebacterium diphtheria ATCC 27012. Corynebacterium diphtheria was isolated from 3 fungi of the genus Trichoderma and Penicillium, with only the genus Trichoderma fungi showing antimicrobial activity.

scholarly journals GC-MS Analysis and Antibacterial Activity of different Solvent Extracts of Shuteria involucrata

2018 ◽  
Vol 7 (2) ◽  
pp. 116-120
Author(s):  
Senthamizh Selvan N ◽  
◽  
Isaiah S ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Disc Diffusion ◽  
Plant Materials ◽  
Gram Negative ◽  
Good Antibacterial Activity ◽  
Ms Analysis ◽  
Diffusion Technique

The present study was focused to examine the presence of phytoconstituents in the ethanolic extract of Shuteria involucrata plant using GC-MS analysis and Antibacterial activity. The GC-MS analysis of S. involucrata leaf was performed using Agilent 6890-JEOL GC-Mate-II Mass Spectrometer. The result of the study showed the presence of six bioactive compounds in the ethanolic extract. The antimicrobial activity was carried out by disc diffusion technique against the four selected pathogens. Among the four, tested for Antibacterial Activity Staphylococcus aureus, and Pseudomonas aeruginosa and were more susceptible to the extract, whereas the others are less susceptible. Ethanol and methanol extracts of plant materials exhibited good antibacterial activity against gram positive, gram negative bacterias

scholarly journals Bacteriocin activity of enterococci and presence of genes related to pathogenesis

2012 ◽  
Vol 30 (No. 4) ◽  
pp. 330-335 ◽  
Author(s):  
K. Trivedi ◽  
R. Sedmíková ◽  
R. Karpíšková
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Listeria Monocytogenes ◽  
Virulence Genes ◽  
Bacteriocin Production ◽  
Bacteriocin Activity ◽  
Antimicrobial Substances ◽  
Layer Technique ◽  
Genes Encoding ◽  
The Individual

In total 228 enterococci strains isolated from food were studied. Enterococcus faecalis, E. faecium, and E. casseliflavus were found to be the dominant strains while E. durans and E. mundtii were present in a smaller extent. Antimicrobial activity determined by double layer technique revealed that 150 (65.7%) strains showed antimicrobial activity against the individual tested pathogenic strains of Listeria monocytogenes, Staphylococcus aureus, and methicilin resistant S. aureus (MRSA). Cell-free neutralised supernatants (CFNS) were prepared from 150 potential bacteriocin producers. Of these 150, CFNS 107 (71.3%) strains were active in the bacteriocin production against one or more pathogenic strains tested. S. aureus and MRSA were found to be more sensitive to the antimicrobial substances than L. monocytogenes. Multiplex PCR for the detection of seven virulence genes in bacteriocin producing strains showed that 47.6% of strains were able to amplify one or more virulence genes. E. faecalis was the most virulent species. The presence of tyrdc gene was seen in all bacteriocin producing strains. None of the strains carried genes encoding the resistance to vancomycin.  

IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

2017 ◽  
Vol 10 (4) ◽  
pp. 283
Author(s):  
Shirly Kumala ◽  
Ng Vini Aprilia ◽  
Partomuan Simanjuntak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Diffusion Method

Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity

scholarly journals Synthesis of chalcone-based pyrimidine derivatives and their antimicrobial activity

2021 ◽  
Vol 57 (4) ◽  
pp. 431-437
Author(s):  
D. S. Martsinkevich ◽  
K. F. Chernyavskaya ◽  
V. A. Tarasevich
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Tropicalis ◽  
Good Activity ◽  
Pyrimidine Derivatives ◽  
Antimicrobial Substances

A series of substituted chalcones and pyrimidine derivatives on their base was synthesized. Obtained compounds were tested for antimicrobial activity and ability to increase activity of known antimicrobial substances. Pyrimidine products shown good activity against Staphylococcus aureus and Candida tropicalis. These compounds also could increase activity of modified polyguanidines against Staphylococcus aureus.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF HONEY AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 41-43
Author(s):  
S. DHANARAJ ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of honey against aerobic and anaerobic bacteria. Methods: Honey is extracted from the honey comb by trained persons. Antimicrobial activity of honey is performed by Agar Cup Diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and Clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to honey whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to honey. Conclusion: Due to its vast antibacterial activity of honey, it can be used along with other antibiotics to increase its efficiency.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF GARLIC AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 44-46
Author(s):  
K. G. DHANUSH ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of garlic against aerobic and anaerobic bacteria. Methods: Antimicrobial activity of garlic is performed by Agar cup diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to garlic whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to garlic. Conclusion: Due to its vast antibacterial activity of garlic, it can be used along with other antibiotics to increase its efficiency.

scholarly journals Isolasi Cendawan Endofit pada Daun Bambu Betung (Dendrocalamus asper Backer.) dan Potensi sebagai Antibakteri Staphylococcus aureus

BioScience ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 37
Author(s):  
Lydia Yohana Safitri
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Plant Tissue ◽  
Plant Secondary Metabolites ◽  
Fungal Endophyte ◽  
Healthy Plant ◽  
Antimicrobial Substances ◽  
Dendrocalamus Asper

Endophytic fungi are microorganisms found in healthy plant tissue but not pathogenic to plants, and produce secondary metabolites same host plant. Secondary metabolites can be used as a drug. The possibility of endophytic fungi are found in bamboo, because bamboo betung (Dendrocalamus asper Backer.) Has high potential as a drug, because it contains flavonoids, and phenolic coumarin. These compounds are secondary metabolites that produce antimicrobial substances that are used in the pharmaceutical field and are found in plant tissue. To avoid excessive use of medicinal plants that could lead to the extinction of these plants, then used the role of endophytic fungi are capable of producing secondary metabolites to the fullest. In addition, the endophytic fungus in plant tissue can be more than one type of fungal endophyte that the higher production of secondary metabolites. This study aims to determine the types of isolates of endophytic fungi on the leaves of Dendrocalamus asper and know the antibacterial potency against bacterial endophyte fungus Staphylococcus aureus. The results showed that there were thirteen isolates of endophytic fungi were isolated from the leaves of Dendrocalamus asper. Ten isolates of endophytic fungi have potential as an antibacterial against S. aureus and three isolates of endophytic fungi did not show any antibacterial activity. Key Word: Endophytic fungi, Secondary metabolites, antibacterial activity.

scholarly journals Antimicrobial Activity of Stenochlaena palustris and Sauropus androgynus in Staphylococcus aureus, Escherichia coli and Candidia albicans

2021 ◽  
Vol 4 (1) ◽  
pp. 32-38
Author(s):  
Lia Yulia Budiarti ◽  
Isnaini Isnaini ◽  
Puteri Dayana ◽  
Norma Sari ◽  
Nur Almira R. S.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Single Infusion ◽  
E Coli ◽  
Antimicrobial Substances ◽  
The Difference ◽  
Combination Preparation ◽  
Young Part

Stenochlaena palustris and Sauropus androgynus are known to contains antimicrobial substances such as flavonoids, saponins and tannins compounds.The purpose of this study was to analyzes the antimicrobial activity of young and old leaf infusions of S. palustris and S. androgynus leaves against Staphylococcus aureus, Escherichia coli and Candida albicans. Analyze the antibacterial activity of a single preparations with a combination preparation of S.palustris (SP) and S.androgynus (SA) leaves infusion against S.aureus, E.coli and C.albicans. Leaves of S.palustris young part (SP1) taken 0-10 cm from shoots and old parts (SP2) 11-20 cm from shoots, while leaves of S.androgynus young part (SA1) leaves number 1 - 10 from the top and the old part (SA2) leaves number 11-20 from the top. The results showed that a single infusion of SP1 75% and SP2 75%, SA1 90% and SA2 90%, and a combination of SP1 75% and SA1 75%, SP2 75% and SA2 75% have the same activity as ampicillin in S.aureus. Single infusion of SP1 90% and SP2 90%, SA1 90% and SA2 90%, combination of SP1 75% and SA1 80% and the combination of SP2 80% and SA2 60% have the same activity as ciprofloxacin in E. coli. Single infusion of SP1 90% and SP2 90%, and a combination of SP1 80% and SA1 80%, SP2 80% and SA2 80% have the same activity as ketoconazole in C.albicans. The difference in activity due to differences in leaf parts used only occurred in E. coli, whereas in S.aureus and C.albicans (p <0.05).

scholarly journals Comparative studies on the antibacterial activities of leaf extracts of Azadirachta indica and Psidium guajava and antibiotics on methicillin and vancomycin resistant Staphylococcus aureus

2017 ◽  
Vol 4 (3) ◽  
pp. 155 ◽  
Author(s):  
Eze C. ◽  
Iroha I. R. ◽  
Eluu S. C. ◽  
Ejikeugwu P. C. ◽  
Iroha C. S. ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Psidium Guajava ◽  
Multidrug Resistant ◽  
Leaf Extracts ◽  
Diffusion Technique ◽  
Test Organisms ◽  
Vancomycin Resistant ◽  
Conventional Antibiotics

Objective: This study investigated the antibacterial activity of the acetone, methanol, and ethanol leaf extracts of Azadirachta indica and Psidium guajava against methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Staphylococcus aureus (VRSA) strains as well as the antimicrobial activity of some conventional antibiotics on these multidrug resistant organisms.Methods: MRSA and VRSA strains were obtained from the culture collection unit of a tertiary hospital in Ebonyi State, Nigeria. The test organisms and the plant extracts were processed using standard microbiology techniques. Kirby-Bauer disk diffusion technique was used to determine the antimicrobial susceptibility profile of the MRSA and VRSA strains to some conventionally used antibiotics; and the antibacterial activity of the plant extracts was evaluated using agar well diffusion technique with three solvents: ethanol, methanol and acetone.Results: It was revealed in this study that the extracts of A. indica and P. guajava demonstrated some level of antimicrobial activity against the test organism at concentrations of 100 mg/ml and 50 mg/ml. Compared to the conventional antibiotics used, the antibiotics showed better antimicrobial activity against the test organisms than the plant extracts which was least active against the MRSA and VRSA strains.Conclusions: Though the A. indica and P. guajava extracts showed some appreciable antimicrobial activity against the MRSA and VRSA strains, the conventional antibiotics produced better antimicrobial action against these multidrug resistant bacteria. The search for novel compounds with putative antimicrobial activity should be stepped up since plants holds the potential for discovering novel drugs.

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