scholarly journals Isolasi Cendawan Endofit pada Daun Bambu Betung (Dendrocalamus asper Backer.) dan Potensi sebagai Antibakteri Staphylococcus aureus

BioScience ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 37
Author(s):  
Lydia Yohana Safitri
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Plant Tissue ◽  
Plant Secondary Metabolites ◽  
Fungal Endophyte ◽  
Healthy Plant ◽  
Antimicrobial Substances ◽  
Dendrocalamus Asper

Endophytic fungi are microorganisms found in healthy plant tissue but not pathogenic to plants, and produce secondary metabolites same host plant. Secondary metabolites can be used as a drug. The possibility of endophytic fungi are found in bamboo, because bamboo betung (Dendrocalamus asper Backer.) Has high potential as a drug, because it contains flavonoids, and phenolic coumarin. These compounds are secondary metabolites that produce antimicrobial substances that are used in the pharmaceutical field and are found in plant tissue. To avoid excessive use of medicinal plants that could lead to the extinction of these plants, then used the role of endophytic fungi are capable of producing secondary metabolites to the fullest. In addition, the endophytic fungus in plant tissue can be more than one type of fungal endophyte that the higher production of secondary metabolites. This study aims to determine the types of isolates of endophytic fungi on the leaves of Dendrocalamus asper and know the antibacterial potency against bacterial endophyte fungus Staphylococcus aureus. The results showed that there were thirteen isolates of endophytic fungi were isolated from the leaves of Dendrocalamus asper. Ten isolates of endophytic fungi have potential as an antibacterial against S. aureus and three isolates of endophytic fungi did not show any antibacterial activity. Key Word: Endophytic fungi, Secondary metabolites, antibacterial activity.

IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

2017 ◽  
Vol 10 (4) ◽  
pp. 283
Author(s):  
Shirly Kumala ◽  
Ng Vini Aprilia ◽  
Partomuan Simanjuntak
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Fatty Acid ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Diffusion Method

Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity. Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity

scholarly journals KAPANG ENDOFIT LAUT DARI TUMBUHAN PESISIR TERONG PUNGO (Solanum sp.) DAN POTENSINYA SEBAGAI ANTIBAKTERI

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Nabila Ukhty
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Staphylococcus Epidermidis ◽  
Inhibition Zone ◽  
Antibacterial Compounds ◽  
Coastal Plant ◽  
The One

Endophytic fungi is the one of the types microbes that lives in the plant tissue. The fungus can produce secondary metabolites potential as a source of antimicrobial and anticancer. The objectives of this study was to the exploration of new antibacterial compounds derived from marine endophytic fungi isolated from coastal plant terong pungo (Solanum sp.). Eight isolates of marine endophytic fungi with different morphology were collected. Endophytic fungus TPL2 was the selected isolate based on antagonism test. The growth curve showed the stationary phase of isolate TPL was on the 9th day to 12th day. Crude extract of endophytic fungi TPL2 showed the antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa with diameter of inhibition zone 4 mm, 6 mm, and 6 mm, respectively for 2 mg extract/well.

scholarly journals Potent Antibacterial Prenylated Acetophenones from the Australian Endemic Plant Acronychia crassipetala

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 487 ◽  
Author(s):  
Trong D. Tran ◽  
Malin A. Olsson ◽  
David J. McMillan ◽  
Jason K. Cullen ◽  
Peter G. Parsons ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
First Record ◽  
Endemic Plant ◽  
Structural Characterisation ◽  
Gram Positive Bacteria ◽  
Spectroscopic Analyses ◽  
Therapeutic Properties ◽  
Endemic Plant Species

Acronychia crassipetala is an endemic plant species in Australia. Its phytochemistry and therapeutic properties are underexplored. The hexane extract of the fruit A. crassipetala T. G. Hartley was found to inhibit the growth of the Gram-positive bacteria Staphylococcus aureus. Following bio-activity guided fractionation, two prenylated acetophenones, crassipetalonol A (1) and crassipetalone A (2), were isolated. Their structures were determined mainly by NMR and MS spectroscopic analyses. This is the first record of the isolation and structural characterisation of secondary metabolites from the species A. crassipetala. Their antibacterial and cytotoxic assessments indicated that the known compound (2) had more potent antibacterial activity than the antibiotic chloramphenicol, while the new compound (1) showed moderate cytotoxicity.

scholarly journals Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Michele Sumampouw
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Potato Dextrose Agar ◽  
Rhizophora Stylosa ◽  
E Coli

Abstract: The aim of this study is to investigate the antibacterial effect of endophytic fungi originated from the root of mangrove Rhizophora stylosa growing on Mangrove Plantation Area around DAS Simpang 5 Jl. Piere Tendean Manado. The method of this research was using combination of PDA and NA media. Two species of endophytic fungi were extracted from the root of R. stylosa, black mycelium fungi as isolate A and brown mycelium fungi as isolate B. The activities of both isolates were tested against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The result showed the same inhibition zone of isolate A and B to S. aureus, while, isolate A showed bigger the inhibition zone in comparison to isolate B against E. coli. As a conclusion, the endophytic fungi taken from the root of R. stylosa have antibacterial activity toward S. aureus and E. coli. Keywords: endophytic fungi, Rhizophora stylosa, antibacterial, Staphylococcus aureus, Escherichia coli.   Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar bakau Rhizophora stylosa yang ditanam di sekitar Perairan Daerah Aliran Sungai Simpang Lima Jl. Piere Tendean Manado. Metode penelitian dilakukan dengan menggunakan kombinasi media Potato Dextrose Agar (PDA) dan Nutrient Agar (NA). Dari akar bakau R. stylosa diperoleh dua  jenis jamur endofit yaitu isolat A dengan karateristik miselium jamur berwarna hitam dan isolat B yang dengan karakteristik miselium berwarna coklat. Kedua isolat ini selanjutnya diujikan aktivitasnya terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan isolat A dan B memberikan  zona hambat yang sama terhadap bakteri Staphylococcus aureus dan pada bakteri Escherichia coli isolat A menunjukkan diameter zona hambat yang lebih besar dibandingkan isolat B. Kesimpulan, Jamur endofit yang diisolasi dari akar bakau R. Stylosa memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan E. coli. Kata kunci: jamur endofit, Rhizophopra stylosa, antibakteri, Staphylococcus aureus, Escherichia coli.

scholarly journals SUBSTANSI ANTIBAKTERI JAMUR ENDOFIT PADA LAMUN ASAL PERAIRAN TONGKAINA

2017 ◽  
Vol 5 (3) ◽  
pp. 83
Author(s):  
Galih Prasetyo ◽  
Remmy Mangindaan ◽  
Robert Antonius Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Antibiotic Activity ◽  
Thalassia Hemprichii ◽  
Enhalus Acoroides ◽  
Rice Medium ◽  
Partition Process

Seagrass is a part of Phanerogamae, commonly has a symbiotic relationship with microbial endophytes. The types of microbes those have ability to produce bioactive compounds with potentially exploited for medical, agriculture and industrial purposes. Antibacterial testing using the modified Kirby-Bauer method. The fungi show a strong antibiotic activity which cultivated statically and Staphylococcus aureus was induced in rice medium for 10 days. The purpose of inducing bacteria to the culture is to stimulate a strong antibiotic activity through Silence Biosynthesis Pathway. Fungal isolates were macerated with 96% of ethanol for 24 hours. Partition process was performed by adding solvents (ethyl acetate, n-hexane, ethanol and water) to get n-hexane, ethanol and water fractions. All fractions were tested their activity against clinical isolates bacteria S. aureus and Escherichia coli. Ten fungal endophytes were isolated from seagrass Thalassia hemprichii and Enhalus acoroides. Two isolates derived from the leaf of both seagrass specimens (E.D.1 and Th.D.1) showed strong antibacterial activity against S. aureus only. Antibacterial activity test of each fraction both active isolates show in water and ethanol fractions. This indicates the active antibacterial compounds of both endophytic fungi have semi-polar and polar characteristics. However, bacterial induction has no effect on their antibacterial activity.Keywords : Endophytic fungi, Thalassia hemprichii, Enhalus acoroides, antibacterial activity Staphylococcus aureus and Escherichia coli.Lamun merupakan tanaman tingkat tinggi yang mempunyai hubungan simbiosis dengan mikroba jamur endofit. Mikroba endofit ini mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif dengan potensi yang besar untuk dieksploitasi dan menghasilkan yang bermanfaat di bidang medis, pertanian, dan industri. Isolasi jamur dilakukan mengacu pada penelitian Bara et al (2013). Pengujian antibakteri dilakukan berdasarkan metode Kirby-Bauer yang dimodifikasi. Jamur memperlihatkan aktivitas antibiotik yang kuat dikultivasi statis dan di induksikan bakteri S. aureus dalam media nasi selama 10 hari. Tujuan pemberian bakteri pada kultur yaitu untuk memicu adanya aktivitas antibiotik yang lebih kuat melalui jalur biosintesis senyap (Silence Biosintethic Pathway) pada jamur tersebut. Isolat jamur di maserasi dengan menambahkan etanol 96% selama 24 jam. Proses partisi dengan menambahkan pelarut (etil asetat, n-heksan, etanol dan air) untuk memperoleh fraksi n-heksan, etanol dan air. Tiap fraksi diuji kembali aktivitas antibiotiknya pada bakteri S. aureus dan Escherichia coli. Hasil penelitian ini diperoleh sepuluh isolat jamur dari lamun T. hemprichii dan E. acoroides. Dua isolat daun (E.D.1 dan Th.D.1) menunjukkan aktivitas yang kuat terhadap bakteri S. aureus. Pengujian aktivitas antibakteri tiap fraksi kedua isolat jamur memperlihatkan fraksi air dan etanol yang menunjukkan aktivitas penghambat. Hal ini menunjukkan bahwa senyawa aktif antibakteri kedua jamur endofit berisfat semi polar dan polar. Induksi bakteri tidak memberikan pengaruh terhadap aktivitas antibakteri.Kata Kunci : jamur endofit, Thalassia hemprichii, Enhalus acoroides, aktivitas antibakteri Staphylococcus aureus dan Escherichia coli.

scholarly journals Antibacterial Activity of Endophytic Fungi from Sembukan (Paederia foetida L.) Leaves

2021 ◽  
Vol 6 (3) ◽  
pp. 189-195
Author(s):  
Hary Widjajanti ◽  
Christina Vivid Handayani ◽  
Elisa Nurnawati
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
In Vitro Tests ◽  
E Coli ◽  
Paederia Foetida

The antibiotic resistance of phatogenic bacteria has become a serious health problem and has encouraged the search for novel and effective antimicrobial metabolites. Meanwhile, endophytic fungi have great potential as a natural source for antimicrobial agents. The endophytic fungi that live in plant tissue produces secondary metabolites which potentially act as an antibacterial compound. The isolation of fungi for antibacterial sources reduces the large amount of plant as a source of antibacterial agents. Hence, this study aims to obtain endophytic fungi isolates from Paederia foetida L. that are capable of producing secondary metabolites as antibacterial, carry out in vitro tests to verify the antibacterial properties of secondary metabolites of the Paederia foetida L. endophytic fungi, and identify the potential of Paederia foetida L. endophytic fungi in producing antibacterial compounds. The antibacterial activity was tested against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 while seven isolates of endophytic fungi that potentially produced antibacterial were obtained from Sembukan (P. foetida L.). The results showed that antibacterial activities of SL1, SL4 and SL6 secondary metabolites against S. aureus ATCC6538 and E. coli ATCC8739 were moderate to strong activities. Furthermore, the Minimum Inhibition Concentration (MIC) of secondary metabolites extract of SL1 against S. aureus ATCC6538 value was 250 ????g/mL while the values of MIC extract of SL4 against S. aureus ATCC6538 and E. coli ATCC8739 were 125 ????g/mL and 250 ????g/mL respectively and MIC extract of SL6 against E. coli ATCC8739 value was 125 ????g/mL. The secondary metabolites extract of SL1 isolate were alkaloid and tannin, SL4 were phenolic and alkaloid while SL6 isolate were alkaloid and terpenoid. Hence, endophytic fungi SL1 isolate was identified as Fusarium sp., SL4 as Dematophora sp., and SL6 isolate as Acremonium sp.

scholarly journals Terpenes from Zingiber montanum and Their Screening against Multi-Drug Resistant and Methicillin Resistant Staphylococcus aureus

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 385 ◽  
Author(s):  
Holly Siddique ◽  
Barbara Pendry ◽  
M. Mukhlesur Rahman
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Mass Spectra ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Drug Resistant ◽  
Two Dimensional ◽  
Methicillin Resistant ◽  
One Dimensional ◽  
Nmr Data

Bioassay directed isolation of secondary metabolites from the rhizomes of Zingiber montanum (Fam. ‎Zingiberaceae) led to the isolation of mono-, sesqui-, and di-terpenes. The compounds were characterized as (E)-8(17),12-labdadiene-15,16-dial (1), zerumbol (2), zerumbone (3), buddledone A (4), furanodienone (5), germacrone (6), borneol (7), and camphor (8) by analysing one-dimensional (1D) (1H and 13C) and two-dimensional (2D) (COSY, HSQC, HMBC, and NOESY) NMR data and mass spectra. Among these terpenes, compounds 1 and 2 revealed potential antibacterial activity (minimum inhibitory concentrations (MIC) values 32–128 µg/mL; 0.145–0.291 mM)) against a series of clinical isolates of multi-drug resistant (MDR) and Methicillin resistant Staphylococcus aureus (MRSA).

scholarly journals Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Ratna Setyaningsih ◽  
Ari Susilowati ◽  
Dina Prasetyani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Its Region ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Bacterial Cells ◽  
Methicillin Resistant

Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl acetate extract of matoa’s endophytic fungi to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also identify isolates having high antibacterial activity. Initial test on antibacterial activity was conducted by using paper disc diffusion, while advanced test was conducted by determining minimum inhibitory concentration (MIC). The fungi were identified in morphological and molecular manner with amplification and sequencing of ITS region. Bacterial cells exposed to the extract were observed by using scanning electron microscope (SEM). Isolate AM72 was found out to have the highest antibacterial activity compared to other 12 isolates tested, with inhibition zone diameter of 31.57 mm to S. aureus and 22.87 mm to MRSA. Isolate AM72 was identified as Lasiodiplodia theobromae. Isolate BMB identified as Aspergillus oryzae produced inhibition zone with 21.96 mm diameter to S. aureus and 21.50 mm diameter to MRSA. Advanced test to isolate AM72 gave the same MIC results to S. aureus and MRSA of 1.56%. Antibacterial compound produced by isolate AM72 were found out to be able to damage bacterial cell walls. Keywords: Antibacterial, Endohpytic Fungi, Matoa, S. aureus, MRSA  

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