Techniques of Quickly Breaking Seed Dormancy of Tilia amurensis

2011 ◽  
Vol 183-185 ◽  
pp. 678-682 ◽  
Author(s):  
Shou Hai Na ◽  
Hai Nan Wang ◽  
Hai Long Shen ◽  
Li Xue Yang
Keyword(s):  
Low Temperature ◽  
Seed Dormancy ◽  
Room Temperature ◽  
Orthogonal Design ◽  
Germination Rate ◽  
Boiling Water ◽  
Germination Time ◽  
Tilia Amurensis ◽  
Breaking Seed Dormancy ◽  
Temperature Water

This paper proposed an orthogonal design L27(313) with four factors(seeds shucked off pericarp, dipped in water, GA3 treatment, 6-BA treatment and stratification)and three levels based on the dormancy of Tilia amurensis seed. The optimal way for quikly breaking seed dormancy was obtained. That was: 1)shucking off the pericarp; 2)scalding seeds for 2s with 100°C boiling water, then soaking in room temperature water for 24h; 3)soaking in 100 mg•L-1 GA3 and 6-BA solution, respectively for 24h; 4)stratification in varying temperature (10-15°C)for 5d, then in low temperature(0-5°C)for 15d. The germination rate of T. amurensis seed could get to 70.2% by above treatment and germination time was shortened to 30 days which was greatly improved compared to previous study.

scholarly journals Effects of seed priming treatments on the germination and development of two rapeseed (Brassica napus L.) varieties under the co-influence of low temperature and drought

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257236
Author(s):  
Zong He Zhu ◽  
Abdul Sami ◽  
Qing Qing Xu ◽  
Ling Ling Wu ◽  
Wen Yin Zheng ◽  
...  
Keyword(s):  
Low Temperature ◽  
Germination Rate ◽  
Seed Priming ◽  
Seed Vigor ◽  
Stress Conditions ◽  
Stem Length ◽  
Activity Levels ◽  
Sod Activity ◽  
Germination Time ◽  
Rape Seedlings

The present study was performed to evaluate the effects of seed priming. This was done by soaking the seeds of two rapeseed cultivars, namely, ZY15 (tolerant to low temperature and drought) and HY49 (sensitive to low temperature and drought), for 12 h in varying solutions: distilled water, 138 mg/L salicylic acid (SA), 300 mg/L gibberellic acid (GA), 89.4 mg/L sodium nitroprusside (SNP), 3000 mg/L calcium chloride (CaCl2), and 30 mg/L abscisic acid (ABA). Primed and non-primed seeds were left to germinate at 15°C and -0.15 MPa (T15W15) and at 25°C and 0 MPa (T25W0), respectively. The results showed that SA, GA, SNP, CaCl2, and ABA significantly improved the germination potential (GP), germination rate (GR), germination index (GI), stem fresh weight (SFW), stem dry weight (SDW), root length (RL), stem length (SL), and seed vigor index (SVI) under T15W15. For ZY15 seeds under T25W0, GA, SNP, CaCl2, and ABA priming reduced the average germination time (96% after 5 days) compared to that of the control (88% after 5 days). For ZY15 seeds under T15W15, SA, SNP, CaCl2, and ABA priming, with respect to the control and water-treated groups, shortened the average germination time (92% after 5 days) compared to that of the control (80% after 5 days). For HY49 seeds under T25W0, GA, SNP, CaCl2, and ABA priming reduced the average germination time (92% after 5 days) compared to that of the control (85% after 5 days). Similarly, for HY49 seeds under T15W15, GA priming shortened the average germination time (89% after 5 days) compared to that of the control (83% after 5 days). These priming agents increased the net photosynthesis, stomatal conductivity, and transpiration rate of rape seedlings under conditions of low temperature and drought stress, while also decreasing intercellular carbon dioxide (CO2) concentrations. Additionally, SA, GA, SNP, CaCl2, and ABA increased superoxide dismutase concentrations (SOD) and ascorbic peroxidase (APX) activities of rape seedlings under stress conditions, while decreasing catalase (CAT) and peroxidase (POD) activities in ZY15 seedlings. In HY49, which is sensitive to low temperature and drought, all priming solutions, except for SNP, led to an increase in SOD activity levels and a decrease in CAT activity levels. Overall, SA, GA, SNP, and CaCl2 increased the concentrations of indoleacetic acid (IAA), GA, ABA, and cytokinin (CTK) in seedlings under stress conditions. Moreover, compared to SA, CaCl2, and ABA, GA (300 mg/L) and SNP (300 mol/L) showed improved priming effects for ZY15 and HY49 under stress conditions.

scholarly journals Pengaruh suhu penyimpanan dan konsentrasi sitokinin terhadap pematahan dormansi benih kentang (Solanum tuberosum L.) G2

Kultivasi ◽  
2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Anne Nuraini ◽  
Sumadi Sumadi ◽  
Yuyun Yuwariah ◽  
Hanifah Rulistianti
Keyword(s):  
Solanum Tuberosum ◽  
Low Temperature ◽  
Seed Dormancy ◽  
Room Temperature ◽  
Storage Temperature ◽  
Solanum Tuberosum L ◽  
Potato Seed ◽  
Seed Potatoes ◽  
Split Plot ◽  
Plot Design

Sari. Kentang (Solanum tuberosum L.) adalah salah satu komoditas yang mendapat prioritas pengembangan, karena produk tanaman ini dipakai sebagai sumber karbohidrat serta memiliki potensi dalam diversifikasi pangan. Salah satu permasalahan dalam produksi kentang adalah terbatasnya persediaan benih kentang, karena adanya fase dormansi. Percobaan ini bertujuan untuk mengetahui interaksi antara suhu penyimpanan dan konsentrasi sitokinin terhadap pematahan dormansi benih kentang. Rancangan percobaan yang digunakan adalah Rancangan Petak Terbagi (Split Plot Design) dengan tiga ulangan.  Petak utama adalah suhu penyimpanan dengan tiga taraf, yaitu: suhu rendah ±10oC , suhu ruang  ±25oC  dan suhu tinggi ±30oC. Anak petak adalah empat taraf konsentrasi sitokinin, yaitu: 0 mgL-1, 50 mgL-1, 100 mgL-1, dan 150 mgL-1. Hasil percobaan menunjukkan terdapat pengaruh interaksi antara suhu penyimpanan dengan konsentrasi sitokinin dalam mempercepat pematahan dormansi benih kentang. Penyimpanan benih kentang pada suhu ruang disertai pemberian konsentrasi sitokinin 50 mgL-1 dapat mempercepat pematahan dormansi benih kentang G2. Perlakuan suhu rendah menghasilkan tunas yang lebih panjang tapi bobotnya tidak berbeda dengan yang diberi perlakuan suhu ruang dan suhu tinggi, sedangkan pengaruh perlakuan sitokinin tidak berbeda terhadap panjang tunas, persentase tumbuh tunas per ubi, dan bobot segar tunas.Kata Kunci : Benih Kentang, Suhu Penyimpanan, Sitokinin, DormansiAbstract. Potato (Solanum tuberosum L.) is one of the important agriculture commodities, because it contains carbohydrates and can use for food diversification in Indonesia. One of the problems in potato production is the limited of seed potatoes, because of potato seed dormancy. This experiment analyzed the interaction between storage temperature and concentration of cytokinin on dormancy breaking of potato seed. The experimental design used Split Plot Design with three replications. The main plot was the temperature of storage, that consisted of three levels: low temperature ± 10°C, room temperature  ± 25°C and high temperature  ± 30oC. Subplot consisted of four levels of cytokinin concentration: 0 mgL-1, 50 mgL-1, 100 mgL-1 and 150 mgL-1. The results of the experiment showed that there was an interaction effect between storage temperature and cytokinin concentration on accelerating the breakdown of potato seed dormancy. Storage of seed potatoes at room temperature with application of 50 mgL-1 cytokinin accelerated the breakdown of G2 potato seed dormancy.  Low temperature treatment resulted longer shoot but the weight was not different than other temperature treatments, whereas cytokinin treatment did not differ in shoot length, percentage of bud growth per seed, and fresh weight of shoots.Keyword: Potato Seed, Storage Temperature, Cytokinin, Dormancy

Effects of temperature and moisture stress on the germination of diverse common bean genotypes

1993 ◽  
Vol 73 (3) ◽  
pp. 697-702 ◽  
Author(s):  
P. Hucl
Keyword(s):  
Phaseolus Vulgaris ◽  
Seed Germination ◽  
Low Temperature ◽  
Common Bean ◽  
Germination Rate ◽  
Moisture Stress ◽  
Phaseolus Vulgaris L ◽  
Percent Germination ◽  
Response To Temperature ◽  
Temperature Water

Seed germination of diverse common bean (Phaseolus vulgaris L.) genotypes was evaluated under controlled suboptimal conditions. Initially, 16 genotypes were tested at 12 and 16 °C. A subset of genotypes was subsequently germinated in distilled water or in polyethylene glycol solutions with water potentials (WP) of either −0.4 or −0.8 MPa at temperatures of 22, 26 or 34 °C. Genotypes did not respond consistently to low temperature stress, as indicated by significant (P < 0.05) crossovers in genotype ranking for percent germination from one temperature (12 °C) to the next (16 °C). Genotypes differed in median germination time (GT50) and estimated maximum germination rate. Genotypes with thinner seeds germinated better than wide-seeded genotypes under low-temperature conditions. Seed germination was reduced by 10 percentage units at a WP of −0.4 MPa and was virtually nil at a WP of −0.8 MPa. GT50 was reduced by 30% at 26 versus 22 °C but delayed by 80% when the WP was lowered from 0 to −0.4 MPa. Similarly, maximum germination rate increased with temperature but decreased with WP. Based on the germplasm evaluated in this study, common bean genotypes differ in seed germination response to temperature and moisture stress. Key words: Common bean, Phaseolus vulgaris L., germination, temperature, water potential

scholarly journals Evaluation of Ferula Assa-Foetida Accessions for Germination Parameters Under Cold Stratification to Overcome Seed Dormancy and Effect of Media Mixtures on Seedling Growth

2020 ◽  
Author(s):  
Ashok Kumar ◽  
Ravi Kumar ◽  
Satbeer Singh ◽  
Sahil Sharma ◽  
Sanatsujat Singh ◽  
...  
Keyword(s):  
Survival Rate ◽  
Seed Dormancy ◽  
Seedling Survival ◽  
Root Diameter ◽  
Leaf Length ◽  
Germination Time ◽  
Mean Germination Time ◽  
Pharmaceutical Industries ◽  
Breaking Seed Dormancy ◽  
Survival Experiment

Abstract Ferula assa-foetida L. is a perennial of Apiaceae family having seed dormancy which inhibits the germination. Oleo-gum resin obtained from the rhizomes of Ferula assa-foetida plant has several medicinal properties and used for the treatment of various diseases, pharmaceutical industries and in cooking of food in some countries. In present study, three different temperature treatments (5°, 15° and 25°C) were used to break seed dormancy in six Ferula assa-foetida accessions repeatedly for two years. Also the seedling survival rate with other morphological parameters like plant height, no. of leaves, leaf width, leaf length, root length and root diameter were observed on 3 month old seedlings in six different media mixtures during year 2019-20. The germination ranges from 3.63 (EC968466 at 25°C) to 81.88 percent (EC966538 at 5°C) with an average of 31.14 percent over all temperatures and genotypes. The mean germination time was ranged from 19.69 to 42.71 days with an average of 30.14 days. The highest germination (66.9%) and least mean germination time was observed at 5ºC (20.85) which showed that this crop required a 5ºC chilling treatment of about 20 days for breaking seed dormancy. The results pertaining to seedling survival experiment showed that media mixture of sand, soil, FYM and cocopeat (1:2:2:1 and 1:1:1:1 ratio) gave higher seedling survival rate (87.66%) and other morphological traits. It was also observed that the genotype EC966538 and EC968470 were the best performer for overall germination as well as seedling survival parameters and could be used as base population in future selection and improvement breeding programs.

Seed Dormancy and Germination in Ecotypes of Johnsongrass

Weed Science ◽  
1969 ◽  
Vol 17 (3) ◽  
pp. 359-361 ◽  
Author(s):  
R. B. Taylorson ◽  
C. G. McWhorter
Keyword(s):  
Low Temperature ◽  
Seed Dormancy ◽  
Seed Coat ◽  
Temperature Regime ◽  
Temperature Shift ◽  
Sorghum Halepense ◽  
Fluorescent Light ◽  
Breaking Seed Dormancy ◽  
Temperature Pretreatment ◽  
Seed Dormancy And Germination

We studied germination of seeds of 44 morphologically distinct, vegetative ecotypes of johnsongrass (Sorghum halepense (L.) Pers.). Seeds of most ecotypes were highly dormant. Without low temperature pretreatment, best germination occurred with continuous fluorescent light, a KNO3 substratum, and an alternating 20 to 35 C temperature regime. Pretreatment of the seeds for 2 weeks at 10 C, followed by a temperature shift to 40 C for 2 hr was effective in breaking seed dormancy. Germination of each of 12 ecotypes, from harvests in each of 2 years, was markedly similar. Interpretation of the evidence suggests that seed dormancy is largely imposed by the relative degree of mechanical restriction afforded by the seed coat.

scholarly journals THE CONTROL OF APRICOT SEED DORMANCY AND GERMINATION BY LOW TEMPERATURE TREATMENTS

2021 ◽  
pp. 11-15
Author(s):  
SAMIR A SEIF EL-YAZAL ◽  
MOHAMED A EI-YAZAL
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Seed Dormancy ◽  
Seedling Growth ◽  
Effective Temperature ◽  
Chemical Constituents ◽  
Germination Percentage ◽  
Local Variety ◽  
Normal Seedling ◽  
Breaking Seed Dormancy

Objective: Freshly harvested seeds of “Local” apricot variety were found to be dormant and did not germinate at all. A specific low-temperature stratification treatment was required to overcome seed dormancy. The most effective temperature for breaking seed dormancy, germination, and seedling growth was 5°C cold stratification (CS). Increased seed germination percentage was recorded when the period of stratification prolonged. Seedling developed from stratified seeds had better growth than those developed from non-stratified seeds. Methods: For stratification treatments, the seeds with removed endocarp were mixed with moistened sand. Afterward, they were subjected to a period of stratification at 5°C. Seeds were stratified in pots of 30 cm×40 cm. Stratified seeds were regularly irrigated once per week. To prevent the water loss during stratification upper surface of pots was covered by a sack. The following stratification was applied for apricot variety: CS for 0, 3, 6, 9, 12, and15 days in 1998 and 1999 years for “Local” variety. Results: Apricot seeds required a CS of about 15 days for “Local” variety to reach maximum germination and normal seedling growth. Moreover, when stratification period was prolonged, some of the chemical constituents of apricot seeds were increased and other was decreased. Therefore, it can be suggested that breaking of dormancy is coincided with several changes in different chemical constituents of seeds. Some of these materials increased (total, reducing and non-reducing sugars, total free amino acids, total indoles, and total and conjugated phenols) and other materials such as free phenols which decreased at seed germinations. Conclusion: The most effective temperature for breaking seed dormancy, germination, and seedling growth was 5°C CS. Increased seed germination percentage was recorded when the period of stratification prolonged. Seedling developed from stratified seeds had better growth than those developed from non-stratified seeds.

Breaking Seed Dormancy in Corchorus olitorius Landraces

Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
AE-D Helaly ◽  
H Al-Amier ◽  
AA Al-Aziz ◽  
LE Craker
Keyword(s):  
Seed Dormancy ◽  
Corchorus Olitorius ◽  
Breaking Seed Dormancy

Survival of mouse blastocysts after low-temperature preservation under high pressure

2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti
Keyword(s):  
Phase Transition ◽  
Hydrostatic Pressure ◽  
Low Temperature ◽  
High Hydrostatic Pressure ◽  
Room Temperature ◽  
Freezing Point ◽  
Significant Loss ◽  
Embryo Cryopreservation ◽  
Subzero Temperatures ◽  
Survival Capacity

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.

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