Synthesis and Antioxidant Activity of Carboxymethyl Chitosan-Selenium

2013 ◽  
Vol 734-737 ◽  
pp. 2100-2103
Author(s):  
Li Ming Jin ◽  
Ning Geng ◽  
Chun Shan Quan ◽  
Sheng Di Fan
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radicals ◽  
Sodium Selenite ◽  
Antioxidant Activities ◽  
Carboxymethyl Chitosan ◽  
Superoxide Radicals ◽  
Organic Selenium ◽  
Antioxidant Effects ◽  
Better Than ◽  
Scavenging Rates

The objective of this study was to synthesize a new organic selenium compound, carboxymethyl chitosan-selenium (CMCTS-Se), and evaluate its antioxidant abilities on hydroxyl radicals and superoxide radicals. Sodium selenite and carboxymethyl chitosan (CMCTS) were used to prepare CMCTS-Se. It was characterized by ultraviolet (UV) and infrared (IR) techniques. The results proved the successful synthesis of CMCTS-Se. The yield was 44.78% and the content of selenium (Se) was 20.50mg/g determined by colorimetry at 420nm using 3.3-diaminobenzidine as color-produced reagent. The antioxidant activities on hydroxyl radicals and super oxide radicals of CMCTS-Se were investigated. The results showed that antioxidant activities were concentration related in the setting concentration range and the antioxidant effects of CMCTS-Se were totally better than that of CMCTS. The scavenging rates on hydroxyl radicals and superoxide radicals of 2.0mg/mL CMCTS-Se were 68.25% and 62.15%, respectively. VC, a well-known antioxidant, as a standard in our assay, showed a little higher antioxidant activity than CMCTS-Se. This research provides a possible application of CMCTS-Se as a low toxic and effective organic Se-enriched healthy product.

Synthesis of Chitooligosaccharide-Selenium and its Antioxidant Activity In Vitro

2013 ◽  
Vol 680 ◽  
pp. 149-153
Author(s):  
Li Ming Jin ◽  
Chen Zhuang ◽  
Chun Shan Quan ◽  
Xiao Jing Zhao ◽  
Sheng Di Fan
Keyword(s):  
Hydroxyl Radicals ◽  
Sodium Selenite ◽  
Chemical Method ◽  
Antioxidant Activities ◽  
Selenium Compound ◽  
Organic Selenium ◽  
Antioxidant Effects ◽  
Better Than ◽  
Scavenging Rates

The objective of this study was to synthesize a new organic selenium compound, chitooligosaccharide-selenium (COSSe), and evaluate its antioxidant abilities on hydroxyl radicals, super oxide radicals and DPPH in vitro. Sodium selenite and chitooligosaccharide (COS) were used to synthesize COSSe by chemical method. The yield was 37.26% and the content of selenium (Se) was 9.02mg/g. COSSe was characterized by ultraviolet (UV) and infrared (IR) techniques. The results proved the successful synthesis of COSSe. The antioxidant activities on hydroxyl radicals, super oxide radicals and DPPH of COSSe were investigated. The results showed that antioxidant activities were concentration related in the setting concentration range and the antioxidant effects of COSSe were totally better than that of COS. The scavenging rates on hydroxyl radicals, superoxide radicals and DPPH of 2.5mg/mL COSSe were 71.19%, 64.54% and 69.78%, respectively. This research provides a possible application of COSSe as a low toxic and effective organic Se-enriched healthy product.

A Study on the Antioxidant Activity and Tissues Selective Inhibition of Lipid Peroxidation by Saponins from the Roots of Platycodon grandiflorum

2009 ◽  
Vol 37 (05) ◽  
pp. 967-975 ◽  
Author(s):  
Xian-Jun Fu ◽  
Hong-Bing Liu ◽  
Peng Wang ◽  
Hua-Shi Guan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Selective Inhibition ◽  
Antioxidant Systems ◽  
Platycodon Grandiflorum ◽  
Platycodi Radix ◽  
Antioxidant Effects ◽  
Different Tissues ◽  
Selective Effects

Platycodi Radix is the root of Platycodon grandiflorum (Jacq.) A. DC and has been used as a traditional medicine in China. According to the theory of traditional Chinese medicine, Platycodi Radix (PR) possesses the character of lung meridian tropism (Guijing) and has selective effects on the lung and respiratory system. The aim of this study was to confirm the antioxidant effects of saponins from Platycodi Radix (PRS), with emphasis on its selective inhibition of lipid peroxidation in different tissues. The hydroxyl radical scavenging activity was determined by a H2O2/Fe2+ system; a modified thiobarbituric acid reactive species assay was used to measure the lipid peroxide in rats' tissues. These antioxidant activities were compared to ascorbic acid (Vc). The results showed that PRS had antioxidant activities in various antioxidant systems. And the inhibition capability of lipid peroxidation of PRS and Vc were excellent, but differed greatly in different tissues. These results suggested that PRS had antioxidant effects and selective inhibition of lipid peroxidation. It indicated that the mechanism of Platycodi Radix to treat some diseases might be related to its antioxidant activity, especially its tissue selective effects. However, further study is needed.

scholarly journals Optimisation of the antioxidant activity of kombucha fermented milk products

2014 ◽  
Vol 32 (No. 5) ◽  
pp. 477-484 ◽  
Author(s):  
R. Malbaša ◽  
J. Vitas ◽  
E. Lončar ◽  
J. Grahovac ◽  
S. Milanović
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radicals ◽  
Milk Fat ◽  
Antioxidant Activities ◽  
Antioxidant Response ◽  
Fermented Milk ◽  
Process Temperature ◽  
Milk Products ◽  
Wild Thyme ◽  
Fermented Milk Products

The antioxidant activity of fermented milk products obtained by using kombucha starter produced by fermentation on sweetened wild thyme extract was investigated. The starter was added to milk containing 0.8, 1.6, and 2.8% milk fat, at fermentation temperature of 37, 40, and 43°C. The fermentation process was terminated when the pH reached 4.5. Antioxidant activities to DPPH and hydroxyl radicals, the contents of MUFAs, PUFAs, vitamin C, and sensory mark, were monitored using the response surface methodology (RSM) and the method of desired function. Kombucha fermented milk products containing wild thyme (WT) showed opposite antioxidant response to DPPH and hydroxyl radicals in terms of milk fat. Optimum processing conditions for WT products in terms of antioxidant activity were: milk fat 2.78% and process temperature 37°C. In order to obtain WT products with a high sensory mark, these conditions are completely different (milk fat 1.10% and process temperature 43°C).  

Evaluation of Antioxidant Activities and Antimutagenicity of Turmeric Oil: A Byproduct from Curcumin Production

2002 ◽  
Vol 57 (9-10) ◽  
pp. 828-835 ◽  
Author(s):  
Guddadarangavvanahally K. Jayaprakasha ◽  
Bhabani S. Jena ◽  
Pradeep S. Negi ◽  
Kunnumpurath K. Sakariah
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Sodium Azide ◽  
Ames Test ◽  
Antioxidant Activities ◽  
Model System ◽  
Oxygenated Compounds ◽  
Antioxidant Effects ◽  
Β Carotene ◽  
Antimutagenic Action

Curcumin removed turmeric oleoresin (CRTO) was extracted with hexane and concentrated to get turmeric oil, and that was fractionated using silica gel column chromatography to obtain three fractions. These fractions were analyzed by GC and GC-MS. Turmeric oil contained aromatic turmerone (31.32%), turmerone (15.08%) and curlone (9.7%), whereas fractions III has aromatic turmerone (44.5%), curlone (19.22%) and turmerone (10.88%) as major compounds. Also, oxygenated compounds (5,6,8-10) were enriched in fraction III. Turmeric oil and its fractions were tested for antioxidant activity using the β-carotene-linoleate model system and the phosphomolybdenum method. The fraction III showed maximum antioxidant capacity. These fractions were also used to determine their protective effect against the mutagenicity of sodium azide by means of the Ames test. All the fractions and turmeric oil exhibited a markedly antimutagenicity but fraction III was the most effective. The antioxidant effects of turmeric oil and its fractions may provide an explanation for their antimutagenic action.

scholarly journals Uji Aktivitas Senyawa Antioksidan dari Ekstrak Metanol Kulit Pisang Raja (Musa paradisiaca Sapientum)

2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Elfira Rosa Pane
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidative Activity ◽  
Antioxidant Activities ◽  
Ethyl Acetate Fraction ◽  
The Other ◽  
Phytochemical Screening ◽  
Musa Paradisiaca ◽  
Ferric Thiocyanate ◽  
Better Than

AbstrakTelah dilakukan penelitian uji aktivitas senyawa antioksidan dari ekstrak metanol kulit pisang raja (Musa paradisiaca Sapientum). Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut metanol. Ekstraksi bertingkat dilakukan terhadap ekstrak metanol dengan n-heksan dan etil asetat. Dari uji fitokimia terhadap fraksi metanol menunjukkan positif flavonoid. Pengujian aktifitas antioksidan dilakukan dengan reaksi oksidasi asam linoleat dengan metoda feritiosianat (FTC) 0,05%. Sebagai standar antioksidan digunakan Butil hidroksianisol (BHA). Hasil penelitian ini menunjukkan ekstrak metanol, fraksi n-heksan dan etil asetat memiliki aktifitas antioksidan yang tidak jauh berbeda dengan BHA. Sedangkan fraksi etil asetat memiliki aktifitas antioksidan yang lebih tinggi daripada BHA.Kata kunci : antioksidan, feritiosianat, Musa paradisiaca SapientumAbstractA research has been done to evaluate the antioxidant activity of metanol extract Musa paradisiaca Sapientum peel’s. Maseration technique was used to get the extract using metanol as solvent. The metanol extract of the peel was re-extracted by solvents into n-hexan and ethyl acetate fractions. Phytochemical screening of metanol extract showed positive flavonoid. The antioxidant activities were tested by using ferric thiocyanate method 0,05% (FTC) on linoleic acid and buthyl hydroxyanisole (BHA) as antioxidant standard. Metanol extract, n-hexan and ethyl acetate fraction exhibited antioxidative activity that was not significantly different from BHA, on the other hand, the ethyl acetate fraction exhibited significant antioxidative activity, which is better than BHA.Keywords : antioxidant, ferric thiocyanate, Musa paradisiaca Sapientum

THE TEST OF ANTIOXIDANT ACTIVITIES WITH COMPARISON OF EXTRACTION METHODS FROM ROBUSTA COFFEE SEEDS (Coffea canephora)

2019 ◽  
Vol 1 (02) ◽  
pp. 60-63
Author(s):  
Novi Fajar Utami ◽  
Nhadira Nhestricia
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Chemical Compounds ◽  
Extraction Methods ◽  
Coffea Canephora ◽  
Robusta Coffee ◽  
Coffee Beans ◽  
Method Of Extraction ◽  
Coffee Seed ◽  
Better Than

The content of robusta coffee include alkaloids, flavonoids, saponin, tannins, caffeine, and phenol. Differences in the method of extraction of a plant can cause differences in the number of chemical compounds. The purpose of this study was to determine the phytochemical characteristics and antioxidant activity in robusta coffee beans extracted by maceration and sonication method. Antioxidant activity was performed by the method of DPPH (2,2-diphenyl-1-picrylhydrazyl) by spectrophotometry. The results showed that phytochemical characteristics of robusta coffee extract of maceration and sonication contain the same chemical compounds, namely alkaloids, flavonoids, tannins, and saponins. The antioxidant activity of robusta coffee seed extract of sonication result has IC50 of 54,14 ppm which is better than the extract of robusta coffee beans from maceration.

scholarly journals Comparative study on the antioxidant activities of ten common flower teas from China

2019 ◽  
Vol 17 (1) ◽  
pp. 841-848
Author(s):  
Xiao-Fang Hu ◽  
Zong-Bao Ding ◽  
Yue Chen ◽  
Jiang-Fu Luo ◽  
Jian-Min He ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Food Industry ◽  
Functional Foods ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Antioxidant Effect ◽  
Total Phenolic ◽  
The World ◽  
Antioxidant Effects

AbstractFlower teas are widely consumed across the world because of their beneficial health effects. The antioxidant activities of methanol extracts from ten common flower teas in China were evaluated using four antioxidant assays. The total phenolic (TPCs) and total flavonoid contents (TFCs) were also investigated. Most of these flower teas exhibited potent antioxidant effects, of which rosae rugosae flos exerted the strongest antioxidant effects in four assays. Rosae rugosae flos also exerted the highest TPC, while fragrans showed the highest TFC. Correction analysis indicated that phenolics play a key role in the antioxidant effect of flower teas, while flavonoids were poorly correlated with antioxidant activity. The results supported the consumption of flower teas as functional foods and their application as sources of natural antioxidants in the food industry.

scholarly journals Synthesis and Antioxidant Activity of Cationic 1,2,3-Triazole Functionalized Starch Derivatives

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 112 ◽  
Author(s):  
Yuan Chen ◽  
Xiguang Liu ◽  
Xueqi Sun ◽  
Jingjing Zhang ◽  
Yingqi Mi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radicals ◽  
Convenient Method ◽  
Click Reaction ◽  
Superoxide Radicals ◽  
Cationic Starch ◽  
Chemically Modified ◽  
Enhanced Solubility ◽  
Starch Derivatives

In this study, starch was chemically modified to improve its antioxidant activity. Five novel cationic 1,2,3-triazole functionalized starch derivatives were synthesized by using “click” reaction and N-alkylation. A convenient method for pre-azidation of starch was developed. The structures of the derivatives were analyzed using FTIR and 1H NMR. The radicals scavenging abilities of the derivatives against hydroxyl radicals, DPPH radicals, and superoxide radicals were tested in vitro in order to evaluate their antioxidant activity. Results revealed that all the cationic starch derivatives (2a–2e), as well as the precursor starch derivatives (1a–1e), had significantly improved antioxidant activity compared to native starch. In particular, the scavenging ability of the derivatives against superoxide radicals was extremely strong. The improved antioxidant activity benefited from the enhanced solubility and the added positive charges. The biocompatibility of the cationic derivatives was confirmed by the low hemolytic rate (<2%). The obtained derivatives in this study have great potential as antioxidant materials that can be applied in the fields of food and biomedicine.

scholarly journals Evaluation of Antioxidant Activity of Spice-Derived Phytochemicals Using Zebrafish

2020 ◽  
Vol 21 (3) ◽  
pp. 1109 ◽  
Author(s):  
Yuka Endo ◽  
Kyoji Muraki ◽  
Yuji Fuse ◽  
Makoto Kobayashi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Specific Property ◽  
Assay System ◽  
Related Factor ◽  
Diallyl Trisulfide ◽  
Dietary Phytochemicals ◽  
Antioxidant Effects

Various dietary phytochemicals seem to display antioxidant activity through the NF-E2-related factor 2 (Nrf2) pathway. However, few studies have demonstrated its antioxidant effect and Nrf2 dependency at the animal level. We constructed a zebrafish-based assay system to analyze the in vivo antioxidant activity of phytochemicals and examined the activity of 10 phytochemicals derived from spices, using this system as a pilot study. Hydrogen peroxide and arsenite were used as oxidative stressors, and Nrf2 dependency was genetically analyzed using an Nrf2-mutant zebrafish line. The activities of curcumin, diallyl trisulfide and quercetin were involved in the reduction of hydrogen peroxide toxicity, while those of cinnamaldehyde, isoeugenol and 6-(methylsulfinyl)hexyl isothiocyanate were involved in the reduction of arsenite toxicity. The antioxidant activities of these phytochemicals were all Nrf2 dependent, with the exception of cinnamaldehyde, which showed strong antioxidant effects even in Nrf2-mutant zebrafish. In summary, we succeeded in constructing an assay system to evaluate the in vivo antioxidant activity of various phytochemicals using zebrafish larvae. Using this system, we found that each spice-derived phytochemical has its own specific property and mechanism of antioxidant action.

Antibacterial and antioxidant effects of Zanthoxylum armatum DC extracts

2021 ◽  
Vol 50 (1) ◽  
pp. 159-164
Author(s):  
Aqsa Irshad ◽  
Irfan Ullah ◽  
Muhammad Fiaz Khan
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibition Zone ◽  
Fruit Extract ◽  
Zanthoxylum Armatum ◽  
Good Antibacterial Activity ◽  
Maximum Inhibition ◽  
Hexane Extracts ◽  
Antioxidant Effects

Antibacterial and antioxidant activities of three different extracts (acetone, methanol, and n-hexane) of Zanthoxylum armatum fruits and leaves were investigated. The maximum inhibition zone was shown by methanolic leaf extract against K. pneumonia (20.51 ± 0.93). The minimum inhibition zone (12.01 ± 0.93) was exhibited by methanolic fruit extract against S. typhi. The highest antioxidant activity was recorded at 1000 ppm. Methanolic leaf extract was the most antioxidant (89.8%) among all the extracts, followed by methanolic fruit extract (88.6%). The methanolic and acetonic extracts showed good antibacterial activity as compared to n-hexane extracts and n-hexane was less antioxidant.

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