Determination of Free Polyethylene Glycol in Cross-Linked Sodium Hyaluronate Gel

2014 ◽  
Vol 881-883 ◽  
pp. 918-922
Author(s):  
Yong Liang Zhao ◽  
Tian Tian Meng ◽  
Wei Guo Wang ◽  
Jia Li Wan
Keyword(s):  
Quality Control ◽  
Polyethylene Glycol ◽  
Standard Deviation ◽  
Linear Relationship ◽  
Recovery Rate ◽  
Detection Method ◽  
Sodium Hyaluronate ◽  
Liquid Samples ◽  
Excellent Stability

Abstract: In this study we present a method for the determination of free polyethylene glycol in a gel. We detect the PEG residues of cross-linked sodium hyaluronate gel by spectrophotometry. The pretreatment of cross-linked sodium hyaluronate gel is special and the plot of working curve is unconventional. The precision, the recovery rate with marker, the reliability and repeatability of this method are tested and verified. The results indicated that the method has an excellent linear relationship in the range of 0-40.5µg mL-1. The regression equation is Y=0.0108X+0.2047 (R2=0.9994). The standard deviation of the detection method is 1.10% (n=9). The recovery rate with marker is 97.8% and the repeatability is perfect. In conclusion, the method possesses the properties of simple operation, quickness, excellent stability. Moreover this method can be applied for the quality control of gel products cross-linked by PEG and liquid samples containing PEG.

Determination of Cl- and SO42- in Boric Acid

2012 ◽  
Vol 152-154 ◽  
pp. 507-512
Author(s):  
Dian Ting Gong
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Boric Acid ◽  
Linear Relationship ◽  
Recovery Rate ◽  
Heating Time ◽  
Measuring Time ◽  
Relative Standard ◽  
Precision And Accuracy

Microcontents of Cl- and SO42- in boric acid were determined by photoelectricity turbidimetry with acid as medium. The effects of stabilizer, acidity of solution, content of precipitant, heating time and temperature as well as measuring time on the turbidity of the solution were studied. After above experiment parameters were optimized, in the measurements to determine SO42- in boric acid, the stabilizer was glycerine-ethanol (1:4), the precipitant was acidic BaCl2. The solution was shaked and heated, as a result, the adding sequence of reagents had no effects on results. The detection limit was 0.10mg/L, relative standard deviation (RSD) was less than 3%, recovery rate was 98.85%~103.00%, and measuring time last over 40min. SO42- concentration in the range of 0.33 mg/L ~0.83mg/L showed a linear relationship with turbidity. In the determination of chlorine, the stabilizer was glycerine, measuring time held in 40min, detection limit was 0.03mg/L, RSD was less than 4%, and recovery rate was 102.2%~106.5%. Cl- concentration in the range of 0.10 mg/L ~0.25mg/L showed a linear relationship with turbidity. Photoelectricity turbidimetry improve the precision and accuracy of the measurements. The method of turbidimetry to determine Cl- and SO42- in boric acid is simple, reliable, innocuous and applicable in practice.

Determination of Nitrate in Water by HPLC

2013 ◽  
Vol 448-453 ◽  
pp. 406-408
Author(s):  
Jing Liu ◽  
Xiao Na Ji ◽  
Qing Kai Ren ◽  
Sheng Shu Ai ◽  
Li Jun Wan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Recovery Rate ◽  
High Performance ◽  
Separation Efficiency ◽  
Fast Analysis ◽  
Relative Standard

We established a method fordetermination of nitrate in water by High Performance Liquid Chromatography(HPLC). The sample was analysed by HPLC-ADA and was quantitated by externalstandard method after being simply processed. This methd has the advantages ofhigh separation efficiency and fast analysis. The experiment result showed thatthe linearly dependent coefficient was0.994, the recovery rate was between 98.7%～105.7%,the relative standard deviation（RSD）wasless than 2.1 %, and the lowest detectable limit is 0.01ng (S/N=1.6).

scholarly journals Aqueous Two-Phase Systems: A New Approach for the Determination of Brilliant Blue FCF in Water and Food Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Sabah Shiri ◽  
Tahere Khezeli ◽  
Shahram Lotfi ◽  
Sina Shiri
Keyword(s):  
Polyethylene Glycol ◽  
Standard Deviation ◽  
Food Samples ◽  
Brilliant Blue ◽  
Phase System ◽  
Optimum Conditions ◽  
Two Phase ◽  
Brilliant Blue Fcf ◽  
Relative Standard

A novel, simple, and more sensitive spectrophotometric procedure has been developed for the determination of brilliant blue FCF in water and food samples by an aqueous two-phase system (ATPS). In this method, adequate amount of polyethylene glycol/ sodium carbonate (PEG-4000/Na2CO3) was added to aqueous solution for formation of a homogeneous solution. To the mixture solution, suitable amount ofNa2CO3was added, the mixture solution was shaken until the salt was dissolved, and then it was separated into two clear phases easily and rapidly. The target analyte in the water sample was extracted into the polyethylene glycol phase. After extraction, measuring the absorbance at 634 nm was done. The effects of different parameters such as polyethylene glycol (type and concentration), pH, salt (type and amount), centrifuge time, and temperature on the ATPS of dye was investigated and optimum conditions were established. Linear calibration curves were obtained in the range of 0.25–750 ng/mL for brilliant blue FCF under optimum conditions. Detection limit based on three times the standard deviation of the blank (3Sb) was 0.12 ng/mL. The relative standard deviation (RSD) for 400 ng/mL was 3.14%. The method was successfully applied to the determination of brilliant blue FCF in spiked samples with satisfactory results. The relative recovery was between 96.0 and 102.2%.

Determination of Uric Acid An Automated Phosphotungstate Method Using NaOH as the Alkali

1968 ◽  
Vol 14 (7) ◽  
pp. 630-636 ◽  
Author(s):  
James L Wheat
Keyword(s):  
Quality Control ◽  
Uric Acid ◽  
Standard Deviation ◽  
Sodium Hydroxide ◽  
Serum Uric Acid ◽  
Good Sensitivity ◽  
Automated Method

Abstract An automated method is described for the determination of serum uric acid using sodium hydroxide alone as the alkalinizing agent in the uric acid-phosphotungstate reaction. Turbidity is not a problem and antiturbidity reagents are not needed. At the rate of 40 samples per hour, there is good sensitivity and good correlation with a manual urea-cyanide method. Quality control shows a standard deviation of ± 0.1 mg./100 ml.

scholarly journals A Novel and Sensitive Method for the Determination of VitaminB2(Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sabah Shiri ◽  
Tahere Khezeli ◽  
Sami Sajjadifar ◽  
Ali Delpisheh ◽  
Moayed Avazpour ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Standard Deviation ◽  
Limit Of Detection ◽  
Vitamin B ◽  
Phase Extraction ◽  
Two Phase ◽  
Pharmaceutical Samples ◽  
Relative Standard ◽  
Aqueous Two Phase Extraction

A novel, simple, and more sensitive spectrophotometric procedure has been developed for the determination of vitamin B2(riboflavin) by an aqueous two-phase extraction (ATPE). An ATPE is formed mostly by water and does not require an organic solvent. Other ATPE components used in this study were the polymer, polyethylene glycol (PEG), and some salts such as Na2SO4and Na2CO3. The method is based on the interaction between vitamin B2(riboflavin) and sodium sulfate (Na2SO4) in an acidic medium (pH 6.4). The influences of effective parameters such as salt (type and concentration), polyethylene glycol (molecular weight and concentration), temperature, centrifuging time, and pH of the sample solution were studied and optimized. The linear range was 1.3–320 ng/mL (R2=0.9991;n=10) with the relative standard deviation (RSD) for 60 ng/mL 3.68%. The limit of detection (LOD) calculated from three times of standard deviation of blank were 0.2 ng/mL and recoveries from analysis of real samples between 94.82% and 103.98% were obtained for the determination of vitamin B2(riboflavin) in urine and pharmaceutical samples.

scholarly journals Evaluation of electropositive filtration for recovering norovirus in water

2011 ◽  
Vol 9 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Heetae Lee ◽  
Misoon Kim ◽  
Soon-Young Paik ◽  
Chan Hee Lee ◽  
Weon-Hwa Jheong ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Recovery Rate ◽  
Detection Method ◽  
Tween 80 ◽  
Environmental Water ◽  
Murine Norovirus ◽  
Virus Adsorption ◽  
Concentration Methods ◽  
Electropositive Filtration ◽  
Surface And Groundwater

The virus adsorption-elution (VIRADEL) technique has been widely used in the recovery of various enteric viruses in water, and an electropositive filter such as 1 MDS has been commonly applied. However, effective methods of monitoring waterborne norovirus (NoV) have not yet been well characterized and optimized. Hence, in this study, the VIRADEL technique was evaluated and optimized for effectively detecting NoV in water by two commonly used electropositive filters (1MDS and NanoCeram filter). Various elution and concentration methods were evaluated by using both murine norovirus (MNV) and human NoV. Among the tested elution buffers, the most effective was 1.5% beef extract plus 0.01% Tween 80 for both 1MDS (67.5%) and NanoCeram (85.7%) microfilters. The recovery rate of GII-4 human NoV was higher by organic flocculation (86.6%) than by polyethylene glycol (PEG) precipitations (11.6~73.6%). When both 1MDS and NanoCeram filters were tested to detect NoV in surface and groundwater, the sensitivity of NoV recovered by these filters appeared to depend on the types and conditions of environmental water. The results of this study will help to set a standard of detection method for NoV in water.

scholarly journals Simultaneous Determination of Nine Active Compounds of the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang and Analysis of the Relationship between Therapeutical Effect and Compatibility of Medicines

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Guangwei Zhu ◽  
Guijun Zhang ◽  
Meng Wang ◽  
Jingjuan Wang ◽  
Weixin Zeng ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Glycyrrhizic Acid ◽  
Detection Method ◽  
Chemical Compounds ◽  
Active Compounds ◽  
Column Temperature ◽  
The Relationship

A simple and sensitive HPLC-DAD detection method was established for the simultaneous determination of nine compounds including oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang (SGT) and we analyze the relationship between therapeutical effect and compatibility of medicines by using an Agilent extend-C18 column at a flow rate of 1 mL/min. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, and glycyrrhizic acid; 276 nm for liquiritin and liquiritigenin; 360 nm for isoliquiritin and isoliquiritigenin. The total contents of the nine compounds in SGT varied from 4.65 to 20.06 mg/mL. The results of this study showed that the content of chemical compounds of Traditional Chinese Medicinal Prescription is mainly influenced by the dosage and compatibility of medicines and the therapeutical effect of Traditional Chinese Medicinal prescription is mainly influenced by the dosage and compatibility of medicines. The method could be suitable for quality control of SGT with bioactive multicompounds.

EDTA Titration of Calcium and Magnesium: Determination of Calcium in Canned Tomatoes

1970 ◽  
Vol 53 (4) ◽  
pp. 720-720
Author(s):  
Edward F Steagall
Keyword(s):  
Quality Control ◽  
Standard Deviation ◽  
Drug Administration ◽  
Food Additives ◽  
Control Program ◽  
Mean Value ◽  
Quality Control Program ◽  
Edta Titration ◽  
Calcium And Magnesium

Abstract This past year a food additives quality control program was established for Food and Drug Administration field laboratories; one phase involved the determination of calcium in canned whole tomatoes. One can of tomatoes containing declared added calcium and one can without added calcium were each analyzed by 18 participating laboratories. Results on the cans with added calcium showed a mean value of 0.031% with a standard deviation of ±0.0032%. Results on t he cans without added calcium showed a mean value of 0.005% with a standard deviation of ±0.0039%.

scholarly journals Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract ofDisporopsis pernyi(Hua) Diels by UPLC

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Yanqi Wang ◽  
Shuyi Li ◽  
Dandan Han ◽  
Kehan Meng ◽  
Miao Wang ◽  
...  
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
Betulinic Acid ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Relative Standard ◽  
Quantitative Basis

Disporopsis pernyi(Hua) Diels, which belongs to genusDisporopsis, has been widely used for the treatment of abnormal sweating, chronic cough, and so forth. An ultra-performance liquid chromatography (UPLC) analysis was developed for the determination of rutin, luteolin, quercetin, and betulinic acid inDisporopsis pernyi(Hua) Diels roots. UPLC analysis was conducted by using a Shim-pack XR-ODS column with gradient elution with the mobile phase of acetonitrile and water containing 0.1% formic acid and with a flow rate of 0.2 mL/min, detected at 210, 254, and 280 nm. The method was precise, with relative standard deviation < 2.0%. The recoveries for the four components inDisporopsis pernyi(Hua) Diels were between 98.5 and 100.9%. The average contents of rutin, luteolin, quercetin, and betulinic acid in roots were 5.63, 2.51, 3.87, and 2.41 μg/g, respectively. The method was accurate and reproducible and it can provide a quantitative basis for quality control ofDisporopsis pernyi(Hua) Diels.

Determination of the International Sensitivity Index of a New Near-Patient Testing Device to Monitor Oral Anticoagulant Therapy

1997 ◽  
Vol 78 (02) ◽  
pp. 855-858 ◽  
Author(s):  
Armando Tripodi ◽  
Veena Chantarangkul ◽  
Marigrazia Clerici ◽  
Barbara Negri ◽  
Pier Mannuccio Mannucci
Keyword(s):  
Linear Relationship ◽  
Whole Blood ◽  
Oral Anticoagulant ◽  
Oral Anticoagulant Therapy ◽  
Oral Anticoagulants ◽  
Calibration Model ◽  
Testing Device ◽  
Data Points ◽  
Near Patient Testing

SummaryA key issue for the reliable use of new devices for the laboratory control of oral anticoagulant therapy with the INR is their conformity to the calibration model. In the past, their adequacy has mostly been assessed empirically without reference to the calibration model and the use of International Reference Preparations (IRP) for thromboplastin. In this study we reviewed the requirements to be fulfilled and applied them to the calibration of a new near-patient testing device (TAS, Cardiovascular Diagnostics) which uses thromboplastin-containing test cards for determination of the INR. On each of 10 working days citrat- ed whole blood and plasma samples were obtained from 2 healthy subjects and 6 patients on oral anticoagulants. PT testing on whole blood and plasma was done with the TAS and parallel testing for plasma by the manual technique with the IRP CRM 149S. Conformity to the calibration model was judged satisfactory if the following requirements were met: (i) there was a linear relationship between paired log-PTs (TAS vs CRM 149S); (ii) the regression line drawn through patients data points, passed through those of normals; (iii) the precision of the calibration expressed as the CV of the slope was <3%. A good linear relationship was observed for calibration plots for plasma and whole blood (r = 0.98). Regression lines drawn through patients data points, passed through those of normals. The CVs of the slope were in both cases 2.2% and the ISIs were 0.965 and 1.000 for whole blood and plasma. In conclusion, our study shows that near-patient testing devices can be considered reliable tools to measure INR in patients on oral anticoagulants and provides guidelines for their evaluation.

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