Immobilization of SA-α-2,6-Gal Receptors Related to Influenza on Magnetic Nanoparticles Coated with Chitosan

2014 ◽  
Vol 976 ◽  
pp. 19-24
Author(s):  
Karla M. Gregorio-Jauregui ◽  
Susana A. Carrizales-Alvarez ◽  
Jorge E. Rivera-Salinas ◽  
Hened Saade ◽  
Jose L. Martinez ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Influenza Infection ◽  
Structural Similarity ◽  
Average Diameter ◽  
Sambucus Nigra ◽  
Functionalized Nanoparticles ◽  
Viral Hemagglutinin ◽  
Host Cell Surface ◽  
Superparamagnetic Properties ◽  
Representative Protein

Influenza infection is carried out due to the virus hemagglutinin recognizes host cell surface with terminal sialic acid (SA) linked receptors SA-α-2,6-Gal for human strain. Sambucus nigra lectin has structural similarity to the viral hemagglutinin. Magnetic nanoparticles (MNP) coated with chitosan can be used as support for the study of these receptors. The goal of this study was to extract the SA-α-2,6-Gal receptors of porcine trachea for their immobilization on magnetic nanoparticles coated with chitosan. The extraction was carried out based on affinity of Sambucus nigra lectin to SA-α-2,6-Gal receptors. It was possible to immobilize up to 76% of SA-α-2,6-Gal receptors, with a molecular weight at 88.4 kDa for more representative protein. The presence of the receptors was confirmed trough FTIR analysis. Magnetic functionalized nanoparticles showed superparamagnetic properties and an average diameter around 10 nm. These results may be used to evaluate the interaction between functionalized nanoparticles and specific lectin or hemagglutinin of influenza virus as model of study virus-receptor.

Fabrication of magnetic amino-functionalized nanoparticles for S-arylation of heterocyclic thiols

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 48980-48985 ◽  
Author(s):  
Yanhong Liu ◽  
Lincheng Zhou ◽  
Xinping Hui ◽  
Zhenwen Dong ◽  
Hao Zhu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Coupling Agent ◽  
Functionalized Nanoparticles ◽  
Polyethylene Imine

The aminosilane coupling agent and polyethylene imine-600 were loaded onto magnetic nanoparticles to obtain magnetic nanoligands (MNLs) A, B, C and D.

Optimizing degradation conditions on treatment of TATB explosive wastewater by γ-Fe2O3 nanoparticles/ UV synergistic degradation

2020 ◽  
Author(s):  
Xiaonan Liu ◽  
Yuedan Deng ◽  
Chaorong Zhang ◽  
xueyuan bai ◽  
Jinshan Li
Keyword(s):  
Magnetic Nanoparticles ◽  
Reaction Time ◽  
Size Distribution ◽  
Uv Light ◽  
Fe2o3 Nanoparticles ◽  
Hydrolysis Method ◽  
Uv Illumination ◽  
Synergistic Degradation ◽  
Optimized Conditions ◽  
Superparamagnetic Properties

In this work, the effect of superparamagnetic γ-Fe2O3 nanoparticles/ ultraviolet light (UV) synergistic degradation on the treatment of 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) explosive wastewater was studied. γ-Fe2O3 nanoparticles were prepared by hydrolysis method and its degradation performance of TATB explosive wastewater was systematically studied with UV light assisted. The results showed that γ-Fe2O3 magnetic nanoparticles have low a size distribution ranged from 5 nm to 10 nm and possesses superparamagnetic properties. The optimized degradation condition was investigated and best degradation performance was obtained with the optimized conditions: the initial of pH=3, UV illumination intensity (5 w/cm2), reaction temperature (25 °C), initial TOC concentration (4.025 mg/L) as well as reaction time (60 min). This work can offer a new idea to degrade the explosive wastewater.

Structural, optical, and magnetic properties of Fe-doped In2O3 nanocubes

2008 ◽  
Vol 23 (10) ◽  
pp. 2597-2601 ◽  
Author(s):  
Dewei Chu ◽  
Yu-Ping Zeng ◽  
Dongliang Jiang ◽  
Zhongming Ren ◽  
Weili Ren ◽  
...  
Keyword(s):  
Solvothermal Method ◽  
Magnetic Measurements ◽  
Mixed Valence ◽  
Average Diameter ◽  
Fe Doping ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic ◽  
Fe Ions ◽  
Superparamagnetic Properties ◽  
Scattering Measurement

Fe-doped In2O3 nanocubes were synthesized by a solvothermal method. The lattice constant a decreases linearly as Fe doping concentration increases, and Raman scattering measurement proves the incorporation of Fe ions into the In2O3 crystal lattice. Mössbauer spectra show the presence of mixed valence of Fe ions instead of Fe3O4, while the sample is superparamagnetic. The products with an average diameter of 80 nm have a single-crystalline phase and appear as a square shape. Magnetic measurements confirm the superparamagnetic properties of the nanocubes, and electron paramagnetic resonance studies indicate Fe ions occupy different sites in the In2O3 matrix.

scholarly journals The Potential Application of Magnetic Nanoparticles for Liver Fibrosis Theranostics

2021 ◽  
Vol 9 ◽  
Author(s):  
Aziz Eftekhari ◽  
Allahveirdy Arjmand ◽  
Ayyub Asheghvatan ◽  
Helena Švajdlenková ◽  
Ondrej Šauša ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Liver Fibrosis ◽  
Hepatic Fibrosis ◽  
Liver Diseases ◽  
Molecular Mechanisms ◽  
Tissue Penetration ◽  
Specific Therapy ◽  
Chronic Liver Damage ◽  
Superparamagnetic Properties ◽  
New Strategies

Liver fibrosis is a major cause of morbidity and mortality worldwide due to chronic liver damage and leading to cirrhosis, liver cancer, and liver failure. To date, there is no effective and specific therapy for patients with hepatic fibrosis. As a result of their various advantages such as biocompatibility, imaging contrast ability, improved tissue penetration, and superparamagnetic properties, magnetic nanoparticles have a great potential for diagnosis and therapy in various liver diseases including fibrosis. In this review, we focus on the molecular mechanisms and important factors for hepatic fibrosis and on potential magnetic nanoparticles-based therapeutics. New strategies for the diagnosis of liver fibrosis are also discussed, with a summary of the challenges and perspectives in the translational application of magnetic nanoparticles from bench to bedside.

scholarly journals Evaluation of BALB/c mice behavior and relations to the immune response after treatment with H3N2 homeopathic solutions

2021 ◽  
Vol 10 (36) ◽  
pp. 128-129
Author(s):  
Camila Monteiro Siqueira ◽  
Leoni Bonamin ◽  
Priscila Motta ◽  
Thayná Cardoso ◽  
Michelle Correia ◽  
...  
Keyword(s):  
Immune Response ◽  
Influenza A Virus ◽  
Influenza A ◽  
Low Cost ◽  
Influenza Infection ◽  
Control Group ◽  
Humoral Immune ◽  
Etiologic Agents ◽  
Viral Hemagglutinin ◽  
Field Device

In Brazil, homeopathic medicines are prepared according to the Homeopathic Pharmacopeia, regulated by ANVISA. Among several categories of medicines, there is the biotherapic group, which is prepared from etiologic agents. In this study, we developed a biotherapic from influenza A virus, aiming the influenza infection prevention. Influenza is a disease that affects thousands of people worldwide every year, with an important economic impact, what motivates the development of new low cost therapies. The H3N2 biotherapic developed in this study was administered to Balb/c mice to evaluate their immune response to viral specific antigens and behavior (homeopathic proving). Sixty-two 4 weeks old Balb/c mice were divided into five experimental groups (n=14 per group), after approval by the Ethics Committee of Animal Use (Protocol DFBCICB 037) and stimulated daily, blindly, with 1% (v/v) different homeopathic medicines, for a maximum period of 42 days. The tested medicines were: biotherapic 30x prepared from inactivated influenza A virus; biotherapic 30x prepared with infectious influenza A virus; and thymulin 5cH, a thymus hormone. The two control groups were treated with water 30x and nothing (baseline group). After 21 days of treatment, half of the animals from each group was challenged subcutaneously with the viral hemagglutinin antigen (7 mg / 200 mL) and monitored by 21 days further, to evaluate the humoral immune response and general behavior, using an open field device. The remaining animals were evaluated by the same behavioral tests at the end of the first 21 days, as an attempt to define the proving features. After euthanasia, all animals were autopsied and the spleen, lungs, heart and mediastine lymph nodes were weighed. Histometry of the spleen follicles was also made. Histopathological and behavioral analyses showed absence of behavioral effects, however, there was increase of spleen lymphoid follicles diameter in immunized animals treated with thymulin and with the biotherapic prepared from infectious influenza A, when compared to the control group. This experiment is being repeated using flow cytometry to complete the analysis and confirm the results.

A versatile large-scale and green process for synthesizing magnetic nanoparticles with tunable magnetic hyperthermia features

RSC Advances ◽  
2016 ◽  
Vol 6 (58) ◽  
pp. 53107-53117 ◽  
Author(s):  
K. Simeonidis ◽  
S. Liébana-Viñas ◽  
U. Wiedwald ◽  
Z. Ma ◽  
Z.-A. Li ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Large Scale ◽  
Magnetic Hyperthermia ◽  
Industrial Scale ◽  
Green Process ◽  
Functionalized Nanoparticles

Production of functionalized nanoparticles for magnetic hyperthermia by an industrial-scale process.

scholarly journals A Comprehensive Updated Review on Magnetic Nanoparticles in Diagnostics

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3432
Author(s):  
Pedro Farinha ◽  
João M. P. Coelho ◽  
Catarina Pinto Reis ◽  
Maria Manuela Gaspar
Keyword(s):  
Magnetic Nanoparticles ◽  
Imaging Techniques ◽  
High Surface ◽  
Volume Ratio ◽  
The Body ◽  
Fe Content ◽  
Low Toxicity ◽  
Magnetic Resonance Imaging Mri ◽  
Imaging Properties ◽  
Superparamagnetic Properties

Magnetic nanoparticles (MNPs) have been studied for diagnostic purposes for decades. Their high surface-to-volume ratio, dispersibility, ability to interact with various molecules and superparamagnetic properties are at the core of what makes MNPs so promising. They have been applied in a multitude of areas in medicine, particularly Magnetic Resonance Imaging (MRI). Iron oxide nanoparticles (IONPs) are the most well-accepted based on their excellent superparamagnetic properties and low toxicity. Nevertheless, IONPs are facing many challenges that make their entry into the market difficult. To overcome these challenges, research has focused on developing MNPs with better safety profiles and enhanced magnetic properties. One particularly important strategy includes doping MNPs (particularly IONPs) with other metallic elements, such as cobalt (Co) and manganese (Mn), to reduce the iron (Fe) content released into the body resulting in the creation of multimodal nanoparticles with unique properties. Another approach includes the development of MNPs using other metals besides Fe, that possess great magnetic or other imaging properties. The future of this field seems to be the production of MNPs which can be used as multipurpose platforms that can combine different uses of MRI or different imaging techniques to design more effective and complete diagnostic tests.

scholarly journals Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Jinhui Cui ◽  
Haixin Cui ◽  
Yan Wang ◽  
Changjiao Sun ◽  
Kui Li ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Gene Transfer ◽  
Gene Delivery ◽  
Fluorescent Protein ◽  
Average Diameter ◽  
Mass Ratio ◽  
Dna Complexes ◽  
Transfer Vector ◽  
Gene Transfer Vector ◽  
Modified Magnetic Nanoparticles

To evaluate the performance of the magnetic nanoparticles as gene transfer vector for breeding transgenic animals, we investigated a new approach to deliver green fluorescent protein (GFP) gene to porcine kidney 15 (PK-15) and porcine embryonic fibroblast (PEF) cells using PEI-modified magnetic nanoparticles as gene vector. The morphology of the nanoparticles and nanoparticle/DNA complexes was characterized using scanning electron microscopy. It was found that the surface of the particles becomes coarse and rough with increased average diameter, which implied the effective conjugating between nanoparticles with DNA. The zeta potential of nanoparticle/DNA complexes drops down from +29.4 mV to +23.1 mV comparing with pure nanoparticles. Agarose gel electrophoresis experiments show that DNA plasmids can be protected effectively against degradation of exonuclease and endonuclease. The efficiency of gene delivery was affected by the mass ratio of nanoparticle/DNA and the amount of nanoparticle/DNA complexes. We confirm that the most optimal mass ratio of nanoparticle/DNA is 1  :  1 by conducting a series of experiments. This work provides important experimental basis for the application of the magnetic nanoparticles on gene delivery to porcine somatic cells, which is significant for the achieving of breeding new transgenic cloned pigs by using somatic cell nuclear transfer technique.

Formation of gold-coated magnetic nanoparticles using TiO2 as a bridging material

2006 ◽  
Vol 21 (5) ◽  
pp. 1312-1316 ◽  
Author(s):  
Brittany L. Oliva ◽  
Anindya Pradhan ◽  
Daniela Caruntu ◽  
Charles J. O'Connor ◽  
Matthew A. Tarr
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Contrast Agents ◽  
Surface Properties ◽  
Blocking Temperature ◽  
Gold Coating ◽  
Magnetic Imaging ◽  
Potential Applications ◽  
Superparamagnetic Properties ◽  
Unique Surface

TiO2 nanoparticles with embedded magnetite were suspended in aqueous HAuCl4 and ultraviolet irradiated to photodeposit gold on the surface. The degree of gold coating and the wavelength of absorbance could be controlled by adjusting [HAuCl4]. Absorbance maxima were between 540-590 nm. Particles exhibited superparamagnetic properties (blocking temperature ∼170 K) whether or not coated with gold. These particles have potential applications as drug delivery agents, magnetic imaging contrast agents, and magnetically separatable photocatalysts with unique surface properties.

Sign in / Sign up

Export Citation Format

Share Document