Evaluation of the Colorimetric Performance of Unmodified Citrate-Stabilized Silver Nanoparticles for Copper (II) Sensing in Water

In this study, the colorimetric performance of unmodified citrate-stabilized silver nanoparticles (cit-AgNP) for Cu2+ detection was investigated. Cit-AgNP was successfully synthesized using the modified Creighton method with sodium borohydride as reducing agent and trisodium citrate as stabilizing agent. The resulting nanoparticle was yellow in color, characteristic of AgNP. The absorbance peak was determined at 400 nm using UV Vis analysis while for morphology, the particles were spherical in shape with an average diameter of 11 nm determined by TEM analysis. In the presence of increasing Cu2+ concentration, the yellow cit-AgNP turned orange and showed decreasing absorbance at 400 nm with simultaneous emergence of additional peak at 450 nm. These changes were attributed to the nanoparticle aggregation confirmed by TEM analysis. A calibration curve generated showed that the absorbance ratio 450/400 nm is directly proportional to Cu2+ concentration from 0 to 40x10-4 M with good linear fit at R2 = 0.9749. The detection and quantification limits were determined to be 6.59x10-4 M and 21.97x10-4 M, respectively. Overall, the study demonstrated the potential of the assay for Cu2+ sensing application.

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Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Scientific Reports ◽

10.1038/s41598-019-50906-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 13

Author(s):

Sudip Some ◽

Onur Bulut ◽

Kinkar Biswas ◽

Anoop Kumar ◽

Anupam Roy ◽

...

Keyword(s):

Silver Nanoparticles ◽

Bombyx Mori ◽

Leaf Extract ◽

Silver Ions ◽

Average Diameter ◽

Face Centered Cubic ◽

Tem Analysis ◽

Beneficial Effects ◽

Selected Area Electron Diffraction ◽

Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

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Synthesis and Characterization of Silver Nanoparticles for Antibacterial Application against Bacillus Subtilis and Pseudomonas Aeruginosa

VNU Journal of Science Mathematics - Physics ◽

10.25073/2588-1124/vnumap.4591 ◽

2021 ◽

Vol 37 (2) ◽

Author(s):

Pham Thi Thu Ha ◽

Vu Xuan Hoa ◽

Trinh Dinh Kha ◽

Nguyen Dac Dien ◽

Luong Duy Thanh ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Silver Nanoparticles ◽

Bacillus Subtilis ◽

Trisodium Citrate ◽

Inhibition Zone ◽

Tem Analysis ◽

High Antibacterial Activity ◽

Antibacterial Performance ◽

Transmission Electron ◽

Zones Of Inhibition

In this study, the stable silver nanoparticles (AgNPs) were synthesized by reducing silver nitrate (AgNO3) using trisodium citrate (TSC). The product was characterized by Ultraviolet-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and X-ray diffraction analysis (XRD). UV-Vis spectrum showed a peak around 420 nm. TEM analysis revealed the homogeneity in the size of AgNPs (35-45 nm), well-dispersed quasi-spherical in water. The prepared AgNPs exhibited high antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa bacteria. The average zones of inhibition were 20 mm and 17 mm for Pseudomonas aeruginosa and Bacillus subtilis bacteria, respectively. The inhibition zone of AgNPs was also compared to the reference antibiotics drugs such as ampicillin and natamycin. This research exhibits an efficient and eco-friendly synthesis of silver nanoparticles with potent antimicrobial and antibacterial performance.

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Green Synthesis of Silver Nanoparticles Using Two Lichens Species: Parmotrema praesorediosum and Ramalina dumeticola

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.229-231.256 ◽

2012 ◽

Vol 229-231 ◽

pp. 256-259 ◽

Cited By ~ 2

Author(s):

Ropisah Mie ◽

Mohd Wahid Samsudin ◽

Laily B. Din ◽

Azizan Ahmad

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Cost Effective ◽

Green Technology ◽

Tem Analysis ◽

Environmental Friendly ◽

Absorbance Peak ◽

Average Size ◽

20 Nm ◽

First Time

Chemical synthetic method in synthesizing silver nanoparticle was quite expensive, toxic and flammable. In order to enhance green technology, we develop a simple biological method for the green synthesis of silver nanoparticles using two lichens species, Parmotrema praesorediosum and Ramalina dumeticola. Silver nanoparticles were characterized using UV-Vis absorption spectroscopy and TEM. Within 72 hours reaction time, absorption spectra of silver nanoparticles formed in R. dumeticola and P. praesorediosum has absorbance peak at 407 nm and 423 nm, respectively. TEM analysis showed the average size of 20 nm of silver nanoparticles obtained in R. dumeticola and the average size of 42 nm of silver nanoparticles obtained in P. praesorediosum. These two lichens species are able to synthesize silver nanoparticles through green chemistry method, which are environmental friendly and cost effective. This is for the first time that any species of lichens was used for the synthesis of silver nanoparticles.

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In situ formation of silver nanoparticles based on dendriticlinear amphiphilic block copolymer micellar templates

e-Polymers ◽

10.1515/epoly.2010.10.1.1289 ◽

2010 ◽

Vol 10 (1) ◽

Author(s):

Huiqing Wang ◽

Ziqiang Shao ◽

Wenjun Wang ◽

Feijun Wang

Keyword(s):

Aqueous Solution ◽

Silver Nanoparticles ◽

Block Copolymer ◽

Average Diameter ◽

Amphiphilic Block Copolymer ◽

Uv Spectrum ◽

H Nmr ◽

Transmission Electron ◽

Convergent Method ◽

Absorbance Peak

AbstractDendritic-linear amphiphilic block copolymer, G1-b-PEG, was synthesized through a convergent method. The first generation benzylic dendritic polyether, G1-Cl, was synthesized, and benzylic dendritic polyether, G1-Cl, was used to react with linear monohydroxyl terminated MPEG in alkaline solution via a convergent method. The copolymer was characterized by 1H-NMR. The amphiphilic block copolymer could self-assemble into micelles in aqueous solution, which were used as templates to prepare dispersed silver nanoparticles in water. The morphology and structure of the hybrids were characterized by transmission electron microscopy (TEM) and ultraviolet spectroscopy (UV), 1H-NMR. An absorbance peak at 406 nm appeared after reduction in the UV spectrum. The average diameter of G1-B-PEG /Ag hybrid micelles was 186 nm, and the sliver nanoparticles are stable in aqueous solution due to the stabilization of the dendritic-linear micelles.

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Microwave Assisted Biosynthesis of Silver Nanoparticles Using the Rhizome Extract of Alpinia galanga and Evaluation of Their Catalytic and Antimicrobial Activities

Journal of Nanoparticles ◽

10.1155/2014/967802 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Siby Joseph ◽

Beena Mathew

Keyword(s):

Silver Nanoparticles ◽

Average Diameter ◽

Antimicrobial Activities ◽

Spectroscopic Studies ◽

Diffusion Method ◽

Tem Analysis ◽

Alpinia Galanga ◽

Microwave Assisted ◽

Physicochemical Methods ◽

Crystalline Nature

Biomediated methods are considered to be a safer alternative to conventional physicochemical methods for the fabrication of nanomaterials due to their eco-friendly nature. In the present study, silver nanoparticles (AgNPs) were synthesized by microwave irradiation using aqueous rhizome extract of the medicinal plant Alpinia galanga. The nanoparticles were also synthesized under ambient condition without the assistance of microwave radiation and the former method was found to be much faster than the latter. The silver nanoparticles were characterized by UV-vis., FTIR, XRD, and HR-TEM analysis. UV-vis. spectroscopic studies provided ample evidences for the formation of nanoparticles. The FTIR spectrum confirmed the presence of plant phytochemicals as stabilizing agent around the AgNPs. XRD and HR-TEM analyses clearly proved the crystalline nature of the nanoparticles. From the TEM images, the nanoparticles were found to be roughly spherical in shape with an average diameter of 20.82 ± 1.8 nm. The nanoparticles showed outstanding catalytic activity for the reduction of methyl orange by NaBH4. The AgNPs were also evaluated for their antimicrobial activity by well diffusion method against S. aureus, B. subtilis, V. cholera, S. paratyphi, and A. niger. They were found to be highly toxic against all the tested pathogenic strains.

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Eco-Friendly Synthesis and Comparative In Vitro Biological Evaluation of Silver Nanoparticles Using Tagetes erecta Flower Extracts

Applied Sciences ◽

10.3390/app12020887 ◽

2022 ◽

Vol 12 (2) ◽

pp. 887

Author(s):

Ana Flavia Burlec ◽

Monica Hăncianu ◽

Irina Macovei ◽

Cornelia Mircea ◽

Adrian Fifere ◽

...

Keyword(s):

Silver Nanoparticles ◽

Synthesis Method ◽

Silver Ions ◽

Average Diameter ◽

Antimicrobial Activities ◽

Biological Evaluation ◽

Tagetes Erecta ◽

Tem Analysis ◽

Synthesis Of Nanoparticles

The present study reports an eco-friendly synthesis method of silver nanoparticles (AgNPs) using two different extracts (aqueous and ethanolic) of Tagetes erecta flowers. When exposed to different biocompounds found in the plant, silver ions are reduced, thus, resulting in the green synthesis of nanoparticles. After performing the optimization of synthesis, the obtained AgNPs were characterized using various techniques. The UV–Vis spectrum of the synthesized nanoparticles showed maximum peaks at 410 and 420 nm. TEM analysis revealed that the particles were spherical with a size ranging from 10 to 15 nm, and EDX analysis confirmed the presence of silver metal. The average diameter value obtained through DLS analysis for the two types of AgNPs (obtained using aqueous and ethanolic extracts) was 104 and 123 nm. The Zeta potentials of the samples were −27.74 mV and −26.46 mV, respectively, which indicates the stability of the colloidal solution. The antioxidant and antimicrobial activities assays showed that nanoparticles obtained using the aqueous extract presented enhanced antioxidant activity compared to the corresponding extract, with both types of AgNPs exhibiting improved antifungal properties compared to the initial extracts.

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Synthesis and Characterization of Silver Nanoplates by a Seed-mediated Method

Communications in Physics ◽

10.15625/0868-3166/29/3/13763 ◽

2019 ◽

Vol 29 (3) ◽

Author(s):

Mai Ngọc Tuan Anh

Keyword(s):

Hydrogen Peroxide ◽

Silver Nanoparticles ◽

Ascorbic Acid ◽

Silver Nitrate ◽

Sodium Borohydride ◽

Trisodium Citrate ◽

Synthesis And Characterization ◽

Silver Nanoplates ◽

Seed Mediated

Silver nanoplates (SNPs) having different size were synthesized by a seed-mediated method. The seeds -silver nanoparticles with 4 – 6 nm diameters were synthesized first by reducing silver nitrate with sodium borohydride in the present of Trisodium Citrate and Hydrogen peroxide. Then these seeds were developed by continue reducing Ag\(^+\) ions with various amount of L-Ascorbic acid to form SNPs. Our analysis showed that the concentratrion of L-Ascorbic acid, a secondary reducing agent, played an important role to form SNPs. In addition, the size and in-plane dipole plasmon resonance wavelenght of silver nanoplates were increased when the concentration of added silver nitrate increased. The characterization of SNPs were studied by UV-Vis, FE-SEM, EDS and TEM methods.

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Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽

10.3390/biology10060473 ◽

2021 ◽

Vol 10 (6) ◽

pp. 473

Author(s):

Prabu Kumar Seetharaman ◽

Rajkuberan Chandrasekaran ◽

Rajiv Periakaruppan ◽

Sathishkumar Gnanasekar ◽

Sivaramakrishnan Sivaperumal ◽

...

Keyword(s):

Silver Nanoparticles ◽

Culture Filtrate ◽

Morphological Changes ◽

Xrd Analysis ◽

Penicillium Oxalicum ◽

Breast Cancer Cell Lines ◽

Face Centered Cubic ◽

Tem Analysis ◽

X Ray ◽

Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

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