scholarly journals Microwave Assisted Biosynthesis of Silver Nanoparticles Using the Rhizome Extract of Alpinia galanga and Evaluation of Their Catalytic and Antimicrobial Activities

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Siby Joseph ◽  
Beena Mathew
Keyword(s):  
Silver Nanoparticles ◽  
Average Diameter ◽  
Antimicrobial Activities ◽  
Spectroscopic Studies ◽  
Diffusion Method ◽  
Tem Analysis ◽  
Alpinia Galanga ◽  
Microwave Assisted ◽  
Physicochemical Methods ◽  
Crystalline Nature

Biomediated methods are considered to be a safer alternative to conventional physicochemical methods for the fabrication of nanomaterials due to their eco-friendly nature. In the present study, silver nanoparticles (AgNPs) were synthesized by microwave irradiation using aqueous rhizome extract of the medicinal plant Alpinia galanga. The nanoparticles were also synthesized under ambient condition without the assistance of microwave radiation and the former method was found to be much faster than the latter. The silver nanoparticles were characterized by UV-vis., FTIR, XRD, and HR-TEM analysis. UV-vis. spectroscopic studies provided ample evidences for the formation of nanoparticles. The FTIR spectrum confirmed the presence of plant phytochemicals as stabilizing agent around the AgNPs. XRD and HR-TEM analyses clearly proved the crystalline nature of the nanoparticles. From the TEM images, the nanoparticles were found to be roughly spherical in shape with an average diameter of 20.82 ± 1.8 nm. The nanoparticles showed outstanding catalytic activity for the reduction of methyl orange by NaBH4. The AgNPs were also evaluated for their antimicrobial activity by well diffusion method against S. aureus, B. subtilis, V. cholera, S. paratyphi, and A. niger. They were found to be highly toxic against all the tested pathogenic strains.

scholarly journals Anti-Urolithiatic Activity of Silver Nanoparticles of Terminalia Chebula Bark

2021 ◽  
Vol 37 (1) ◽  
pp. 109-115
Author(s):  
S. Vidhya ◽  
A. Leema Rose ◽  
F. Janeeta Priya ◽  
T. Keerthana ◽  
R. Priyadharshini
Keyword(s):  
Silver Nanoparticles ◽  
Xrd Analysis ◽  
Spectroscopic Studies ◽  
Modern Medicine ◽  
Diffusion Method ◽  
Terminalia Chebula ◽  
Ft Ir ◽  
Crystalline Nature ◽  
Ir Spectroscopic ◽  
Gel Diffusion

The potential inhibitory action of herbals plays an indispensable role in modern medicine. The struvite crystal is an infectious stone related to urinary tract infection, hence the present study highlighted the anti-urolithiatic potential of Terminalia chebula bark mediated silver nanoparticles. Silver nanoparticles formation was confirmed by SPR band at 437nm. FT-IR spectroscopic studies suggested that phytoconstituents are primarily responsible for the reduction and capping of silver nanoparticles. TEM and XRD analysis revealed that the uniformity in shape and crystalline nature of T. chebula mediated silver nanoparticles. The single gel diffusion method was adopted to found the inhibition action of T.chebula mediated silver nanoparticles in struvite crystals. The inhibition efficiency was found to be 87.9 %, indicated that T. chebula mediated silver nanoparticles was a good inhibitor for the struvite crystals. The shift in band values of FTIR spectra confirms that the phyto constituents present in T. chebula bark is responsible for the anti-urolithiatic activity.

scholarly journals Eco-Friendly Synthesis and Comparative In Vitro Biological Evaluation of Silver Nanoparticles Using Tagetes erecta Flower Extracts

2022 ◽  
Vol 12 (2) ◽  
pp. 887
Author(s):  
Ana Flavia Burlec ◽  
Monica Hăncianu ◽  
Irina Macovei ◽  
Cornelia Mircea ◽  
Adrian Fifere ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Synthesis Method ◽  
Silver Ions ◽  
Average Diameter ◽  
Antimicrobial Activities ◽  
Biological Evaluation ◽  
Tagetes Erecta ◽  
Tem Analysis ◽  
Synthesis Of Nanoparticles

The present study reports an eco-friendly synthesis method of silver nanoparticles (AgNPs) using two different extracts (aqueous and ethanolic) of Tagetes erecta flowers. When exposed to different biocompounds found in the plant, silver ions are reduced, thus, resulting in the green synthesis of nanoparticles. After performing the optimization of synthesis, the obtained AgNPs were characterized using various techniques. The UV–Vis spectrum of the synthesized nanoparticles showed maximum peaks at 410 and 420 nm. TEM analysis revealed that the particles were spherical with a size ranging from 10 to 15 nm, and EDX analysis confirmed the presence of silver metal. The average diameter value obtained through DLS analysis for the two types of AgNPs (obtained using aqueous and ethanolic extracts) was 104 and 123 nm. The Zeta potentials of the samples were −27.74 mV and −26.46 mV, respectively, which indicates the stability of the colloidal solution. The antioxidant and antimicrobial activities assays showed that nanoparticles obtained using the aqueous extract presented enhanced antioxidant activity compared to the corresponding extract, with both types of AgNPs exhibiting improved antifungal properties compared to the initial extracts.

scholarly journals Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sudip Some ◽  
Onur Bulut ◽  
Kinkar Biswas ◽  
Anoop Kumar ◽  
Anupam Roy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Silver Ions ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Beneficial Effects ◽  
Selected Area Electron Diffraction ◽  
Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

scholarly journals Multifaceted Phytogenic Silver Nanoparticles by a Rare Insectivorous Plant Drosera Spatulata Labill Var.bakoensis and its Enhanced Antioxidant, Antibacterial, Antifungal and in Vitro Cytotoxic Studies

2021 ◽  
Author(s):  
Susmila Aparna Gaddam ◽  
Venkata Subbaiah Kotakadi ◽  
Gunasekhar. Kalavakunta ◽  
Josthna Penchalaneni ◽  
Varadarajulu Naidu Challagundla ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Radical Scavenging ◽  
Human Colon ◽  
Xrd Analysis ◽  
Antimicrobial Activities ◽  
Human Colon Cancer ◽  
Tem Analysis ◽  
Insectivorous Plant ◽  
Average Size

Abstract The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var.bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of their high negative zeta potential (-34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.

scholarly journals Microwave Assisted Green Synthesis of Silver Nanoparticles using Pomegranate Peel Extract: Characterization and Antibacterial Activity Studies

2019 ◽  
Vol 32 (2) ◽  
pp. 458-462
Author(s):  
Gugulothu Yaku ◽  
Bandi Rajkumar ◽  
T.V.D. Prasad Rao
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Pomegranate Peel ◽  
Stabilizing Agent ◽  
X Ray ◽  
Microwave Assisted ◽  
Pomegranate Peel Extract

In the present work, a simple and low cost and eco-friendly technique is applied for the microwave assisted synthesis of silver nanoparticles (AgNPs) using the extract of pomegranate peel wastes, which does not require any use of external stabilizing agent. The extract of pomegranates peelings waste served as a reducing as well as capping/stabilizing agent. The synthesized silver nanoparticles were characterized by using powder X-ray diffraction (XRD), transmission eelectron microscopy (TEM), FT-IR, UV-visible spectroscopy and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX). The stability of AgNPs was analyzed by zeta potential measurements. The antibacterial activity of synthesized AgNPs was also evaluated on six Gram-positive and Gram-negative bacteria using agar well diffusion method.

Evaluation of the Colorimetric Performance of Unmodified Citrate-Stabilized Silver Nanoparticles for Copper (II) Sensing in Water

2019 ◽  
Vol 821 ◽  
pp. 372-378
Author(s):  
Francis Eric P. Almaquer ◽  
Jem Valerie D. Perez
Keyword(s):  
Silver Nanoparticles ◽  
Trisodium Citrate ◽  
Average Diameter ◽  
Nanoparticle Aggregation ◽  
Tem Analysis ◽  
Additional Peak ◽  
Linear Fit ◽  
Sensing Application ◽  
Absorbance Peak ◽  
Detection And Quantification

In this study, the colorimetric performance of unmodified citrate-stabilized silver nanoparticles (cit-AgNP) for Cu2+ detection was investigated. Cit-AgNP was successfully synthesized using the modified Creighton method with sodium borohydride as reducing agent and trisodium citrate as stabilizing agent. The resulting nanoparticle was yellow in color, characteristic of AgNP. The absorbance peak was determined at 400 nm using UV Vis analysis while for morphology, the particles were spherical in shape with an average diameter of 11 nm determined by TEM analysis. In the presence of increasing Cu2+ concentration, the yellow cit-AgNP turned orange and showed decreasing absorbance at 400 nm with simultaneous emergence of additional peak at 450 nm. These changes were attributed to the nanoparticle aggregation confirmed by TEM analysis. A calibration curve generated showed that the absorbance ratio 450/400 nm is directly proportional to Cu2+ concentration from 0 to 40x10-4 M with good linear fit at R2 = 0.9749. The detection and quantification limits were determined to be 6.59x10-4 M and 21.97x10-4 M, respectively. Overall, the study demonstrated the potential of the assay for Cu2+ sensing application.

Green Synthesis of Silver Nanoparticles using the Leaves Extract of Filicium decipiens and its Anti- Microbial Activity

2021 ◽  
Vol 10 (3) ◽  
pp. 16-24
Author(s):  
Sherin Monichan ◽  
P. Mosae Selvakumar ◽  
Christine Thevamithra ◽  
M. S. A. Muthukumar Nadar ◽  
Jesse Joel
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Average Diameter ◽  
Diffusion Method ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Almost All

Silver nanoparticles has been used since ages, even till now it is exploited in almost all areas like medicine, textiles, industries, cosmetics, purification, dying and many more. There are many approaches which are used to synthesize silver nanoparticles. However, these approaches are either harmful to the environment or very costly. Therefore, green synthesis of silver nanoparticles (AgNPs) using leaves of Filicium decipiens eco-friendly and a very reliable method to procure AgNPs. Characterization of synthesized AgNPs were then done using UV-Vis spectroscopy and fluorescence which confirmed the formation of AgNPs, scanning electron microscope (SEM)confirmed its shape to be round and X-ray diffraction (XRD) determined its crystalline nature as face centered cubic structure. Furthermore, Dynamic Light Scattering (DLS) was also done in order to know the average diameter and zeta potential of AgNPs. However, it did not show potential results due to the aggregates formed during the green synthesis of AgNPs. In addition to this, anti-microbial test against bacteria such as gram negative (Escherichia. Coli) and gram positive (Bacillus.spc) were done using well-diffusion method and also its application of antimicrobial activity was tested over fabric to understand its application in textile industries. In both the cases, AgNPs showed more efficiency in gram negative bacteria than gram- positive.

scholarly journals Antimicrobial Potential of Biosynthesized Silver Nanoparticles by Aaronsohnia factorovskyi Extract

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 130
Author(s):  
Fatimah Al-Otibi ◽  
Reem A. Al-Ahaidib ◽  
Raedah I. Alharbi ◽  
Rana M. Al-Otaibi ◽  
Gadah Albasher
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Plant Extracts ◽  
Fusarium Solani ◽  
Large Scale ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method

The green biosynthesis of nanoparticles by plant extracts is an attractive and promising technique for medicinal applications. In the current study, we chose one of the daisy plants, Aaronsohnia factorovskyi (which grows in the Najd region, Saudi Arabia), to investigate its anti-microbial efficacy, in combination with silver nanoparticles. The biosynthesized nanoparticles were evaluated for antibacterial activity against Staphylococcus aureus, Bacillussubtilis (Gram-positive), Pseudomonas aeruginosa, and Escherichia coli, (Gram-negative) using the disc diffusion method, while the antifungal activity was assessed against Fusarium oxysporum, Fusarium solani, Helminthosporiumrostratum, and Alternariaalternata. The potential phytoconstituents of the plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques, the Field emission scanning electron microscopy (FE-SEM), Chromatography/Mass Spectrometry (GC-MS) techniques, and Zeta potential analysis. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average diameter of 104–140 nm. Biogenic Aaronsohnia factorovskyi-silver nanoparticles (AF-AgNPs) showed significant antibacterial activity against Staphylococcus aureus with inhibition zone diameter to 19.00 ± 2.94 mm, and antifungal activity against Fusarium solani, which reduced the growth of fungal yarn to 1.5 mm. The innovation of the present study is that the green synthesis of NPs, which is simple, cost-effective, provides stable nano-materials, and can be an alternative for the large-scale synthesis of silver nanoparticles.

scholarly journals Biogenic Nanosilver Mediated by Coir, Medicinal Plant Extracts and their Antimicrobial Validation

CORD ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 18
Author(s):  
Geethanjali Kanagaraju
Keyword(s):  
Staphylococcus Aureus ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Plant Extracts ◽  
Research Work ◽  
Antimicrobial Activities ◽  
Tem Analysis ◽  
Sem And Tem ◽  
Medicinal Plant Extracts ◽  
Biosynthesized Agnps

Knowing the magnitude of therapeutic plants such as Micrococca mercurialis (MM), Abutilon palmeri (AP) and Callistemon citrinus (CC), we also planned to extend our research work on Cocusnucifera fibers (CF) and dust (CD) as it has copious medicinal properties. The current study deals with the green synthesis of silver nanoparticles (AgNPs) from fresh aqueous extracts and AgNO3 solution. The biogenic conversion of silver ion to silver is relatively expeditious at two different time intervals and pH. The isolated  silver nanoparticles (AgNPs) from the bio extracts were identified initially by scrutinizing the colour variations. The biosynthesized AgNPs were characterized by UV, XRD, Laser Diffraction Particle size Analyser, fluorescence spectroscopy, SEM and TEM. UV absorbance at 435-460nm for silver nanoparticles was observed for the above extracts. The XRD pattern of all extracts showed the distinctive Bragg peaks of face centre cubic (fcc) crystalline system available in nature. SEM and TEM analysis of the silver nanoparticles indicated that the particle size was in the range of 2-100nm with polygonal and spherical shapes. The biosynthesized AgNPs were evaluated for antimicrobial activities. The CFAgNPs showed an efficient antibacterial activity at lower concentration (25mg/ml) against Pseudomonas demolytica followed by Staphylococcus aureus and Escherichia coli. Similarly all plant extracts have better activity against fungal strains. It is concluded that the biogenic blend of AgNPs is simple, extremely rapid, beneficial, eco-friendly and more stable without any toxic effects. Of these, CFAgNps may be used for the preparation of antibacterial groupings against Pseudomonas demolytica and Staphylococcus aureus.

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