scholarly journals Investigation of some endogenous antimicrobial peptides in thalassemia

2018 ◽  
Author(s):  
Arif Mustafa Efendiyev ◽  
Gulnara Ibrahim Azizova ◽  
Arzu Ramiz Dadashova
Keyword(s):  
Antimicrobial Peptides ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Immune Status ◽  
Alanine Transaminase ◽  
Blood Samples ◽  
The Third ◽  
Heterozygous Form ◽  
Conjugated Bilirubin ◽  
Homozygous Form

The aim of this work was a comparative study of the amount of antimicrobial peptides - human neutrophil peptides - defensins (HNP), hepcidin, bactericidal/permeability-increasing protein (BPI), and endotoxin in β-thalassemia. Blood samples of 135 patients with thalassemia were investigated. All patients were divided into 3 groups. The first group included patients with heterozygous form (n=45). The second group consisted of patients with homozygous form before splenectomy (n=45). The third group included patients with homozygous form after splenectomy (n=45). The age of patients varied from 2 to 18 years. Biochemical [unconjugated and conjugated bilirubin, alkaline phosphatase, hemoglobin, ferritin, aspartate transaminase (AST), alanine transaminase (ALT), mean corpuscular volume (MCV)] and immune (IgA, IgM, IgG, phagocytic activity) parameters were defined. Obtained results suggest that increased levels of endogenous antimicrobial peptides are associated with the development of the infectious process and reflect the dynamics of changes in biochemical parameters and immune status.

scholarly journals Influence of Pesticide on Biochemical and Hematological Parameters in Beninese Vegetable Farmers

2018 ◽  
Vol 9 (1) ◽  
pp. 65
Author(s):  
Lucie AYI-FANOU
Keyword(s):  
Blood Cell ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Study Group ◽  
Blood Samples ◽  
Vegetable Farmers ◽  
Insignificant Difference ◽  
Hematological And Biochemical Parameters

In several countries, a lot of researches have shown the toxicity of pesticides on farmers. Meanwhile, few of them have dealt with vegetable farmers in Benin. Therefore, this study has been carried out to assess the effects of pesticides on vegetable farmers’ health. This study is conducted on 30 vegetable farmers who used pesticides and 20 subjects as control group. Pesticides used by vegetable farmers and their risky behavior were investigated. Their blood samples were collected and some tests were performed for hematological and biochemical parameters. For the statistical analysis of the results, Student’s test was used. Our investigations revealed that vegetable farmers of Benin were exposed to different active ingredients of pesticides such as (Abamectin, Acetamiprid, Chlorpyriphos ethyl, Cyfluthrin, Cypermethrin, Emamectin benzoat, Flubendiamid, Lambdacyhalothrin, Mancozeb, Profenofos, Spinosad and Spirotétramate). The hematological parameters showed significant decrease in White blood cell (WBC), Red blood cell (RBC), Hemoglobin (HGB), Hematocrit (HTC), Mean Corpuscular Volume (MCV), Platelet (PLT) count among study group. The enzymatic activities of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) have significantly raised between vegetable farmers and the control group, while the serum concentration in urea and creatinine showed insignificant difference in the study group. These results suggest that pesticides have adverse effects on vegetable farmers of South of Benin.

scholarly journals Hematological and biochemical reference values of Asian house shrews (Suncus murinus) in Bangladesh

2019 ◽  
Vol 12 (9) ◽  
pp. 1514-1518
Author(s):  
Md. Kaisar Rahman ◽  
Shariful Islam ◽  
Mizanur Rahman ◽  
Jinnat Ferdous ◽  
Sazeda Akter ◽  
...  
Keyword(s):  
Reference Values ◽  
Biochemical Parameters ◽  
Leukocyte Count ◽  
Clinical Chemistry ◽  
Alanine Transaminase ◽  
Total Leukocyte Count ◽  
Suncus Murinus ◽  
Blood Samples ◽  
Calcium Phosphorus ◽  
Hematological And Biochemical Parameters

Background and Aim: Determining reference values for hematological and biochemical parameters of Asian house shrew (Suncus murinus) is important for wildlife research to protect human health in surrounding communities. This study aimed to establish the reference values for selected hematology and serum clinical chemistry analyses that may contribute to research on shrew in future. Materials and Methods: Blood samples (n=51) were collected from shrews between July and December 2015, Bangladesh, to estimate the levels of hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), total erythrocyte count (TEC), lymphocyte, monocyte, neutrophil, eosinophil, basophil, calcium, phosphorus (P), sodium (Na), chloride (Cl), urea, glucose, total protein (TP), creatinine, and alanine transaminase (ALT). Results: Although the values did not differ significantly among sexes, age was found to be a significant factor. Hb, PCV, TEC, glucose, and P were higher in males; eosinophil, Na, Cl, TP, and ALT were higher among females. Adults had significantly greater urea and glucose (p<0.05) while juveniles had insignificantly higher values for TLC, PCV, neutrophil, P, and TP. Conclusion: This study provides the first reference values for this species in Bangladesh and can be used to guide wildlife research studies.

scholarly journals The Effect of Adding Cumin Cyminym Seeds to Concentrated Feed on Some Blood Biochemical Parameters of Black Local Goats

2020 ◽  
Vol 13 (1) ◽  
pp. 101-105
Author(s):  
Amal Awad ◽  
Mohamed Shwayel
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Jugular Vein ◽  
Control Group ◽  
College Of Agriculture ◽  
Blood Samples ◽  
Blood Biochemical Parameters ◽  
The Third ◽  
Blood Biochemical ◽  
Cumin Seed

The current study was conducted the effect of adding cumin cyminym seeds to concentrated feed on some blood biochemical parameters of black local goats. Fifteen female black goats aged 3 years, third milk production season. The study was in a private animal field of the Department of Animal Production- College of Agriculture/ University of Diyala, during the period from 1/11/2018 to 16/4/2019. The goats were divided randomly into three equal groups, 5 for each group. The first group (T1) left without treatment as a control group. The second group (T2) was given 10 g of cumin seed powder to the concentrated feed/ animal per day. The third group (T3) was given 20 g of cumin seed powder to the concentrated feed/ animal per day. Blood samples were collected before the experiment (Zero day) and at the end of the experiment (3 months), 10 ml from jugular vein by via vacationer tubes without anticoagulant. Blood biochemical parameters were also estimated includes: Total protein, Albumin, Globulin, Glucose, Urea and Triglycerides. The results showed no significant (P>0.05) in Total protein, Albumin, Globulin, Glucose, Urea and Triglycerides between (Zero day) and at the end of the experiment (3 months) and between different treated groups during the study period. It was concluded from this study that addition of cumin seed powder to the black local goats ration have no effects on some blood biochemical parameters.

scholarly journals A Novel ZRS Mutation Leads to Preaxial Polydactyly Type 2 in a Heterozygous Form and Werner Mesomelic Syndrome in a Homozygous Form

2014 ◽  
Vol 35 (8) ◽  
pp. 945-948 ◽  
Author(s):  
Julia E. VanderMeer ◽  
Reymundo Lozano ◽  
Miao Sun ◽  
Yuan Xue ◽  
Donna Daentl ◽  
...  
Keyword(s):  
Preaxial Polydactyly ◽  
Heterozygous Form ◽  
Homozygous Form

scholarly journals Natalizumab concentrations during pregnancy in three patients with multiple sclerosis

2021 ◽  
pp. 135245852110521
Author(s):  
Alyssa A Toorop ◽  
Theo Rispens ◽  
Eva MM Strijbis ◽  
Bob W van Oosten ◽  
Brigit A de Jong ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Disease Activity ◽  
Third Trimester ◽  
Blood Samples ◽  
The Third ◽  
Trough Concentrations ◽  
Active Multiple Sclerosis

In women with very active multiple sclerosis (MS), natalizumab can be continued during pregnancy to prevent rebound disease activity. Our aim was to evaluate changes in serum natalizumab trough concentrations during pregnancy. Blood samples of 3 patients were collected before, during, and after pregnancy. Natalizumab trough concentrations gradually decreased during pregnancy. The patient with the lowest trough concentrations during the third trimester was treated with extended interval dosing (EID). After delivery, natalizumab concentrations increased to similar levels as before pregnancy. All patients remained clinically and radiologically stable. MS neurologists should be aware of decreasing natalizumab concentrations during pregnancy, especially in patients with low initial trough concentrations and patients with EID.

scholarly journals Serum biochemical properties of Vietnamese indigenous Noi chicken at 56 days old

2020 ◽  
Vol 36 (3) ◽  
pp. 329-339
Author(s):  
Nguyen Giang ◽  
Nguyen Hien ◽  
Huynh Loan ◽  
Phan Phuc ◽  
Nguyen Dai ◽  
...  
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Animal Health ◽  
Correlation Coefficients ◽  
Biochemical Properties ◽  
Blood Samples ◽  
Male And Female ◽  
Native Breed ◽  
Serum Biochemical ◽  
The Relationship

Serum profile is generally considered as comprehensive data which directly reflected animal health and their potential resistance to environmental, nutritional and pathological stress. The present study aimed to provide physiological reference values for selected biochemical parameters in Noi chickens, a famous Vietnamese native breed, at 56 days old. The collected blood samples of 355 Noi birds (164 males and 191 females) were used to evaluate biochemical serum profile. Glucose, total protein, albumin, globulin, albumin/globulin ratio, triglycerides, cholesterol, creatinine, and uric acid in the serum were measured. The variation in these values arising from different sexes was also investigated. The results indicated that there were no differences (P>0.05) in the assessed indices between male and female Noi chickens. In overall for the whole group, these values were obtained as 250.86 mg/dL, 7.34 g/dL, 3.40 g/dL, 3.94 g/dL, 1.75, 148.54 mg/dL, 190.86 mg/dL, 0.44 mg/dL, and 1.44 mg/dL, respectively. The relationship among the biochemical parameters showed relatively low coefficients (r=0.79 in maximum). The females obtained higher correlation coefficients between levels of total protein and glucose (r=0.42) as well as between total protein and globulin (r=0.79), compared to the males. It was concluded that the male and female chickens performed similar values of serum indices. The contribution of this study might expand the knowledge on the biochemical profile and improved breeding strategies of Vietnamese indigenous Noi chickens.

scholarly journals Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits

2020 ◽  
Vol 8 (2) ◽  
pp. 205-212
Author(s):  
Tunji Akande ◽  
Timothy Olugbenga Ogundeko ◽  
Solomon Oyewale Opajobi ◽  
Samaila Garba Yaura ◽  
Isiaiah Iorkyase Idyu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Aqueous Extract ◽  
Azadirachta Indica ◽  
Total Bilirubin ◽  
Alanine Transaminase ◽  
The Body ◽  
High Rate ◽  
Care Needs ◽  
Conjugated Bilirubin

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.

scholarly journals Effects of Hamo NK hard capsule on experimental atherosclerosis model

2021 ◽  
Vol 141 (5) ◽  
pp. 95-103
Author(s):  
Pham Thuy Phuong ◽  
Pham Thi Van Anh ◽  
Dang Thi Thu Hien ◽  
Nguyen Trong Thong ◽  
Pham Quoc Binh
Keyword(s):  
Oral Administration ◽  
Triglyceride Level ◽  
High Fat Diet ◽  
Biochemical Parameters ◽  
Serum Triglyceride ◽  
Experimental Atherosclerosis ◽  
Serum Triglyceride Level ◽  
High Fat ◽  
Blood Samples ◽  
Aortic Artery

This study evaluated the effects of Hamo NK hard capsule on athresclerosis using experimental atherosclerosis model. NewZealand White rabbits were fed a high-fat diet (HFD) containing cholesterol and peanut oil. The animals received oral administration of HFD and Hamo NK hard capsule at two doses of 0.126 and 0.378 g/kg bw/day for 8 consecutive weeks. Blood samples were collected for analyis of biochemical parameters at before treatment, week 4 and week 8. Histopathology assessments of the aortic artery and liver were carried out at the end of the experiment. Hamo NK was effective in reducing serum triglyceride level after 8 weeks of the experiment. In addition, Hamo NK at two doses of 0.126 g/kg b.w and 0.378 g/kg b.w for 8 consecutive weeks did not affect the cholesterol, LDL-C and HDL-C concentrations induced by a HFD. Hamo NK at the dose of 0.126 g/kg bw/day was not only able to decrease significant aortic surface lesions but also capable of managing atherosclerosis plaques formation in aorta; whereas theses activities were not notiaceable at the dose of 0.378 g/kg b.w.

scholarly journals Six-Month Physical Activity Versus Sclerostin and Interleukin 6 Concentration in Patients Receiving Renal Replacement Therapy by Hemodialysis – Pilot Study

2020 ◽  
Author(s):  
Agnieszka Turoń-Skrzypińska ◽  
Grazyna Dutkiewicz ◽  
Malgorzata Marchelek-Mysliwiec ◽  
Violetta Dziedziejko ◽  
Krzysztof Safranow ◽  
...  
Keyword(s):  
Physical Activity ◽  
Renal Replacement Therapy ◽  
Kidney Disease ◽  
Replacement Therapy ◽  
Biochemical Parameters ◽  
The Third ◽  
Reduced Physical Activity ◽  
Level Of Significance ◽  
The Relationship ◽  
Lower Physical Activity

Background: Chronic kidney disease and renal replacement therapy are associated with reduced physical activity, which may result in the presence of mineral-bone disorders and an increase in inflammation markers. The aim of the study was to assess the relationship between the performance of daily physical activity, expressed in the number of steps performed by patients undergoing hemodialysis and the concentration of selected biochemical parameters (SCL, IL-6). Materials and Methods: The study involved 33 patients aged 59.8 &plusmn; 9.8 years from the dialysis station at the Department of Nephrology, Transplantology and Internal Medicine PUM. Group C consisted of 30 people aged 54.9 (9.37), with GFR over 60 mL/min/1.73m. The study group was divided into S-N and N-S. Participants of the S-N group were assigned the task of performing the appropriate daily number of steps, and the N-S group was to perform spontaneous physical activity. The tasks were replaced after the third month of the research project. Physical activity was measured with pedometers. Anthropometric and biochemical parameters were assessed at baseline, after the third and sixth month of the study. Descriptive statistics, intergroup comparisons using Mann-Whitney U or Kruskal-Wallis tests and Spearman correlation analysis were performed. The level of significance was set at p&le;0.005. Results: Patients from the S-N and N-S groups compared to group C performed significantly fewer steps in the 0-3 month and 4-6 month periods. In the S-N group, with an increase in steps performed in the period of 4-6 months, sclerostin levels dropped in the 6th month. In group C, the concentration of SCL and IL-6 decreased with the increase in the number of steps taken. Only in group C the waist circumference decreased with the increase of the number of steps performed. Conclusions: Patients receiving renal replacement therapy by hemodialysis showed significantly lower physical activity compared to people without kidney disease. Performing bigger number of steps can lower sclerostin levels in hemodialysis patients.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

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