Prevalence of brucellosis in the human, livestock and wildlife interface areas of Serengeti National Park, Tanzania

Between 2005 and 2006, a cross-sectional survey was carried out in domestic ruminants in agropastoral communities of Serengeti district, Tanzania to determine the seroprevalence of brucellosis in domestic–wildlife interface villages. Both the Rose Bengal Plate Test (RBPT) and Competitive Enzyme Linked-immunosorbent Assay (c-ELISA) were used to analyse 82 human and 413 livestock sera from four randomly selected villages located along game reserve areas of Serengeti National Park. Although both cattle (288) and small ruminants (125) were screened, seropositivity was detected only in cattle. The overall seroprevalence based on c-ELISA as a confirmatory test was 5.6%. In cattle both age and sex were not statistically associated with brucellosis seropositivity (P = 0.63; 95% CI = 0.03, 0.8 and 0.33; 95% CI = 0.6, 3.7, respectively). Overall herd level seropositivity was 46.7% (n = 7), ranging from 25% to 66.7% (n = 4–10). Each village had at least one brucellosis seropositive herd. None of the 82 humans tested with both RBPT and c-ELISA were seropositive. Detecting Brucella infection in cattle in such areas warrants further investigation to establish the circulating strains for eventual appropriate control interventions in domestic animals.

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Peste des Petits Ruminants Virus Infection at the Wildlife–Livestock Interface in the Greater Serengeti Ecosystem, 2015–2019

Viruses ◽

10.3390/v13050838 ◽

2021 ◽

Vol 13 (5) ◽

pp. 838

Author(s):

Bryony A. Jones ◽

Mana Mahapatra ◽

Daniel Mdetele ◽

Julius Keyyu ◽

Francis Gakuya ◽

...

Keyword(s):

Small Ruminants ◽

Viral Disease ◽

Global Strategy ◽

Enzyme Linked Immunosorbent Assay ◽

Weighted Estimate ◽

Peste Des Petits Ruminants ◽

African Buffalo ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Serengeti Ecosystem

Peste des petits ruminants (PPR) is a viral disease of goats and sheep that occurs in Africa, the Middle East and Asia with a severe impact on livelihoods and livestock trade. Many wild artiodactyls are susceptible to PPR virus (PPRV) infection, and some outbreaks have threatened endangered wild populations. The role of wild species in PPRV epidemiology is unclear, which is a knowledge gap for the Global Strategy for the Control and Eradication of PPR. These studies aimed to investigate PPRV infection in wild artiodactyls in the Greater Serengeti and Amboseli ecosystems of Kenya and Tanzania. Out of 132 animals purposively sampled in 2015–2016, 19.7% were PPRV seropositive by ID Screen PPR competition enzyme-linked immunosorbent assay (cELISA; IDvet, France) from the following species: African buffalo, wildebeest, topi, kongoni, Grant’s gazelle, impala, Thomson’s gazelle, warthog and gerenuk, while waterbuck and lesser kudu were seronegative. In 2018–2019, a cross-sectional survey of randomly selected African buffalo and Grant’s gazelle herds was conducted. The weighted estimate of PPRV seroprevalence was 12.0% out of 191 African buffalo and 1.1% out of 139 Grant’s gazelles. All ocular and nasal swabs and faeces were negative by PPRV real-time reverse transcription-polymerase chain reaction (RT-qPCR). Investigations of a PPR-like disease in sheep and goats confirmed PPRV circulation in the area by rapid detection test and/or RT-qPCR. These results demonstrated serological evidence of PPRV infection in wild artiodactyl species at the wildlife–livestock interface in this ecosystem where PPRV is endemic in domestic small ruminants. Exposure to PPRV could be via spillover from infected small ruminants or from transmission between wild animals, while the relatively low seroprevalence suggests that sustained transmission is unlikely. Further studies of other major wild artiodactyls in this ecosystem are required, such as impala, Thomson’s gazelle and wildebeest.

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Seroprevalence and potential risk factors of bovine brucellosis at the livestock-wildlife interface area of Yankari game reserve, Bauchi State, Nigeria.

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i4.292 ◽

2020 ◽

Vol 46 (4) ◽

pp. 33-44

Author(s):

Y. Ibrahim ◽

H. M. Mai ◽

D. J. U. Kalla ◽

J. Kabir ◽

J. Nathaniel

Keyword(s):

Risk Factors ◽

Potential Risk ◽

Age Groups ◽

Confirmatory Test ◽

Enzyme Linked Immunosorbent Assay ◽

Interface Area ◽

Indigenous Cattle ◽

Cross Sectional ◽

Game Reserve ◽

Potential Risk Factors

A cross-sectional study was conducted to determine the seroprevalence and associations with potential risk factors of brucellosis in indigenous cattle breeds at the livestock-wildlife interface area of Yankari Game Reserve, Bauchi State, Nigeria. A total of 1000 sera samples were examined from 44 herds using convenient and simple random sampling technique. Rose Bengal Plate Test (RBPT) and competitive Enzyme-linked Immunosorbent Assay (c-ELISA) were used as screening and confirmatory tests respectively. Of the 1000 samples, 23.5% samples were RBPT positive, and after the confirmatory test, the overall true animal-level prevalence was 4.1%. For the herd level prevalence, (47.7%) herds had at least one animal that is seropositive following both tests. Brucellosis seroprevalence was significantly associated with the following potential risk factors; herds that belong to a cooperative society member (p<0.042), herds that keep only cattle (p<0.001), herds that practice commercial farming system (p<0.011), herds that possess a herd size of 51 to 100 cattle (p?0.009) and herds that have no history of abortion and other reproductive disorder (p<0.009). No statistically significant differences in brucellosis seroprevalence was obtained within sex, breed and age groups It can be concluded that brucellosis is still endemic in the study area.

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Threats to the Serengeti Herds

Oryx ◽

10.1017/s0030605300018111 ◽

1982 ◽

Vol 16 (5) ◽

pp. 437-444 ◽

Cited By ~ 4

Author(s):

Stephen Makacha ◽

Michael J. Msingwa ◽

George W. Frame

Keyword(s):

National Park ◽

Serengeti National Park ◽

Conservation Area ◽

Fast Growing ◽

Game Reserve ◽

The Right ◽

Growing Population

The Serengeti National Park in Tanzania is famous for its huge herds of migrating wildebeest, zebras and other ungulates. But these herds spend much of the year in neighbouring reserves where their survival depends on preserving the right conditions. The authors made a study of two of these reserves with disturbing results. The Maswa Game Reserve they found was seriously threatened by invading (illegal) settlement with a fast-growing population cultivating land and felling trees; in the Ngorongoro Conservation Area they report that the Maasai have taken to poaching, both for subsistence meat and for trophies to sell – skins, ivory and rhino horn. In both places the guards are so poorly equipped they can do little to stop poaching.

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KNOWLEDGE MANAGEMENT LIFECYCLE AND ORGANISATIONAL DECISION MAKING OF PAINT MANUFACTURING COMPANIES IN RIVERS STATE, NIGERIA

Research Journal of Management Practice ◽

10.46654/rjmp.1312 ◽

2021 ◽

pp. 34-55

Author(s):

Odiri EDEBIRI ◽

Anthonia BESTMAN

Keyword(s):

Decision Making ◽

Knowledge Management ◽

Rank Order ◽

Threshold Level ◽

Primary Data ◽

Confirmatory Test ◽

Manufacturing Companies ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Rivers State

This study investigated the influence ofknowledge management lifecycle on organisational decision making of paint manufacturing companies in Rivers State. The study was operationalized with the dimensions of knowledge management lifecycle as knowledge identification, knowledge creation, knowledge storage and knowledge transfer. Organizational decision making was measured by timeliness and cost effectiveness.The target population of this study is all 51 registered paint manufacturing companies in Rivers State. Purposive sampling was used to select a sample of 90 respondents from 30 selected manufacturing companies in Rivers State. The study used cross-sectional survey approach and descriptive research design.The study was guided by five research questions and ten research hypotheses. Questionnaires were the major instrument used in gathering primary data which were analyzed using correlational analyses. The questionnaire used was subjected to face/content validity, this was done to ascertain the validity of the questionnaires to be administered. The scale used for this study had been previously adjudged reliable. However, we also checked by verifying reliability outcomes through confirmatory test of internal consistency on the instrument with our sample using Cronbach alpha at the threshold level 0.7 which is generally accepted by the rule of thumb. Data was analyzed and results presented in tables, mean and standard deviation. And, finally hypotheses were tested using the Spearman Rank Order Correlation Coefficient. The study found that knowledge management lifecycle significantly impact on organisational decision making. The study recommends that in order for paint manufacturing companies in Rivers State to maintain their hedge in this high rivalry economy, they should pay more attention to how knowledge within the organisation are effectively managed as this will facilitate an effective time management and cost optimisation within the organisation.

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Serological Evidence of Henipavirus among Horses and Pigs in Zaria and Environs in Kaduna State, Nigeria

Advances in Biology ◽

10.1155/2015/632158 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Olaolu T. Olufemi ◽

Jarlath U. Umoh ◽

Asabe A. Dzikwi ◽

Yemisi O. Olufemi

Keyword(s):

Rna Virus ◽

Close Contact ◽

Nipah Virus ◽

Hendra Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Public Health Implication ◽

Commercial Feed ◽

Associated Risk Factors

Henipavirus is an emerging, zoonotic, and lethal RNA virus comprising Hendra virus (HeV) and Nipah virus (NiV), to which fruit bats are reservoir. Husbandry practices in Nigeria allow close contact between bat reservoir and animals susceptible to Henipavirus. This cross-sectional survey investigated antibodies reactive to Henipavirus sG antigen and associated risk factors in horses and pigs in Zaria, Nigeria. Using convenience sampling, 510 sera from horses (n=200) and pigs (n=310) were screened by an indirect Henipavirus enzyme-linked immunosorbent assay (ELISA) (CSIRO, Australia). Structured questionnaires were employed with questions on the demographics and management of the animals. Data were analysed using SPSS-17. 5. Seroprevalence was higher for horses managed intensively (21.1%); used for sports (25.5%); watered with pipe borne water (17.9%); fed commercial feed (22.3%); and fed in the pen (17.6%). Seroprevalence was higher for pigs managed intensively (58.1%); imported (69.5%); watered with pipe-borne water (31.3%); fed commercial feed (57.4%); fed in the pen (23.4%), and fed with feed prestored in a feed house (49.5%). Horses <5 years and pigs <6 months had higher seroprevalences of 18.1% and 21.3%, while the female horses and pigs had seroprevalences of 19.8% and 22.8%, respectively. Exotic horses and pigs revealed 25.5% and 55% and horses in Igabi and pigs in Giwa revealed 24.7% and 70.2% seroprevalence, respectively (P<0.05). There is a suggestive evidence of Henipavirus in horses and pigs in Zaria, Nigeria, with a huge public health implication. Local and exotic pigs and horses, pigs in Zaria and Sabon-Gari, and horses in Zaria, Sabon-Gari, and Kaduna North are associated with the seroprevalence of henipaviruses.

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The prevalence and risk factors for human Brucella species infection in a cross-sectional survey of a rural population in Punjab, India

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1093/trstmh/trz133 ◽

2020 ◽

Vol 114 (4) ◽

pp. 255-263 ◽

Cited By ~ 2

Author(s):

Punam Mangtani ◽

Isha Berry ◽

Wendy Beauvais ◽

Hannah R Holt ◽

Amit Kulashri ◽

...

Keyword(s):

Risk Factors ◽

Bovine Milk ◽

Buffalo Milk ◽

Enzyme Linked Immunosorbent Assay ◽

Infection Prevalence ◽

Milk Consumption ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Goat’S Milk ◽

Goat's Milk

Abstract Background Brucellosis is an important neglected zoonosis. Effective cattle vaccines are available but are infrequently used in India, where rural households commonly own one or two cattle as sources of protein and income. We assessed the prevalence of infection and risk factors in humans. Methods We conducted a cross-sectional sero-survey in randomly selected individuals in 60 villages in Punjab. Infection prevalence was assessed by positive Rose Bengal testing or immunoglobulin G enzyme-linked immunosorbent assay. Risk factors were adjusted for potential confounding using multivariable analyses. Results Of the 1927 subjects who were approached, 93% agreed to participate. Age-standardised prevalence for Brucella infection was 2.24% (95% confidence interval [CI] 1.61 to 3.11). More than 60% of households kept cattle and 10% assisted with calving or abortions. Nearly all individuals consumed boiled cow/buffalo milk from their own or neighbours' cattle and 3.3% consumed goat’s milk. There was a 2.18 times increased odds (95% CI 0.96 to 4.95) of infection with calving/abortions and a 4.26 times increased odds (95% CI 1.33 to 13.6) with goat’s milk but not bovine milk consumption. Conclusions An association with calving/abortions and goat’s milk consumption was seen. Brucella vaccination of household livestock would reduce the risk to humans in such settings. Additional measures include biosecurity training around calving/abortions, education to boil all milk and for healthcare workers to test for brucellosis.

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Anaemia and iron deficiency during pregnancy in rural Bangladesh

Public Health Nutrition ◽

10.1079/phn2004645 ◽

2004 ◽

Vol 7 (8) ◽

pp. 1065-1070 ◽

Cited By ~ 24

Author(s):

SM Ziauddin Hyder ◽

Lars-Åke Persson ◽

Mushtaque Chowdhury ◽

Bo Lönnerdal ◽

Eva-Charlotte Ekström

Keyword(s):

Iron Deficiency ◽

Antenatal Care ◽

Pregnant Women ◽

Economic Status ◽

Venous Blood ◽

Haemoglobin Concentration ◽

Severe Anaemia ◽

Enzyme Linked Immunosorbent Assay ◽

Cross Sectional Survey ◽

Cross Sectional

AbstractObjective:To study the prevalence of anaemia and its association with measures of iron deficiency (ID) among a group of pregnant women.Design:Cross-sectional survey.Setting:Pregnant women identified through house-to-house visits and participating in community-based antenatal care activities in a rural location of Mymensingh, Bangladesh.Subjects:The estimates are based on 214 reportedly healthy pregnant women in their second trimester. Information on socio-economic status and reproductive history were obtained through home visits and venous blood samples were collected at antenatal care centres. Haemoglobin concentration (Hb) was measured by HemoCue, serum ferritin (sFt) by radioimmunoassay and serum transferrin receptor (sTfR) by enzyme-linked immunosorbent assay methods. ID was defined as presence of either low sFt (<12 μg l−1) or high sTfR (>8.5 mg l−1).Results:The prevalence of anaemia (Hb <110 g l−1) was 50%, but severe anaemia (Hb >70 g l−1) was absent. Low sFt was observed in 42%, high sTfR in 25%, either low sFt or high TfR in 54% and both low sFt and high TfR in 13% of the pregnant women. Two out of three anaemic women had an indication of ID, which was present in 80% of women with moderate (Hb 70–99 g l−1) and 50% with mild (Hb 100–109 g l−1) anaemia. Four out of 10 non-anaemic women (Hb >110 g l−1) also had ID, but the prevalence was significantly lower than that observed in anaemic women (P=0.001).Conclusions:Despite the high prevalence of anaemia, severe cases were absent. The prevalence of ID increased at lower Hb. However, an increased prevalence was also found among women in the highest category of Hb.

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Adherence to vector preventive measures decrease cases of acute Dengue among Abuja residents, Nigeria

Microbiologia Medica ◽

10.4081/mm.2017.6704 ◽

2017 ◽

Vol 32 (2) ◽

Author(s):

Idris Abdullahi Nasir ◽

Adamu Babayo ◽

Muhammad Sagir Shehu ◽

Peter Omale Musa

Keyword(s):

Dengue Virus ◽

Preventive Measures ◽

Indoor Residual Spraying ◽

Enzyme Linked Immunosorbent Assay ◽

Statistical Association ◽

Virus Infections ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Blood Samples ◽

Preventive Practices

Background: Nigeria is one of the dengue hyper-endemic nations. This study investigated the level of knowledge about dengue and vector preventive practices and their impacts on acute dengue among febrile patients at Abuja, Nigeria. Materials and methods: This cross-sectional survey was conducted on febrile patients attending University of Abuja Teaching Hospital, Abuja, Nigeria. Blood samples were individually collected from 171 febrile patients residing at Gwagwalada suburb. Interviewer administered questionnaires were used to assess subjects’ knowledge about preventive measures against vector breeding and bites. Blood samples were tested for dengue virus Nonstructural glycoprotein-1antigen using enzyme linked Immunosorbent assay (ELISA). Results: Fifteen out of 171 febrile persons (8.8%) were Dengue NS1 positive. Sixty percent of the subjects do not know about dengue, while 33% knew about dengue virus infection through television/ radio programs, 5% through healthcare professionals and 2% from friends/families. Those who persistently use indoor residual spraying and long sleeves/trousers during daytime had less cases of DENV NS1 than those who do not. There was statistical association between DENV NS1 and residence in proximity to waste dumpsites (P<0.0001) and frequent use of long sleeve clothing and trousers (P=0.005). However, there was no statistical association between DENV NS1 antigenemia and persistent use of indoor residual spraying and presence of in-door water containers (P>0.05). Conclusions: Findings from this study imply that proper education and adherence to preventive measures minimize people from being susceptible to Dengue virus infections.

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Outbreak investigation and control case report of brucellosis: Experience from livestock research centre, Mpwapwa, Tanzania

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v81i1.818 ◽

2014 ◽

Vol 81 (1) ◽

Cited By ~ 4

Author(s):

Gabriel M. Shirima ◽

Seleman N. Masola ◽

Obeid N. Malangu ◽

Brant A. Schumaker

Keyword(s):

Clinical Signs ◽

Dairy Farms ◽

Control Measures ◽

Research Centre ◽

Confirmatory Test ◽

Medical Attention ◽

Enzyme Linked Immunosorbent Assay ◽

And Control ◽

The Rose ◽

S19 Vaccine

Brucellosis screening was conducted between 2005 and 2010 at the National Livestock Research Institute headquarters, Mpwapwa, Tanzania, following an abortion storm in cattle. The initial screening targeted breeding herds; 483 cattle were screened using the Rose Bengal Plate Test (RBPT) followed by the Competitive Enzyme-linked Immunosorbent Assay (c-ELISA) as a confirmatory test. The seropositivity on c-ELISA was 28.95% in 2005; it subsequently declined to 6.72%, 1.17%, 0.16% and 0.00% in 2006, 2007, 2009 and 2010, respectively. Brucella seropositivity was not detected in goats. Seropositivity declined following institution of stringent control measures that included: gradual culling of seropositive animals through slaughter; isolation and confinement of pregnant cows close to calving; proper disposal of placentas and aborted foetuses; the use of the S19 vaccine; and restricted introduction of new animals. It was thought that the source of this outbreak was likely to have been from the introduction of infected animals from another farm. Furthermore, humans were found with brucellosis antibodies. Out of 120 people screened, 12 (10%) were confirmed seropositive to brucella antigen exposure by c-ELISA analysis. The majority of the seropositive individuals (80%) were milkers and animal handlers from the farm. Nine individuals had clinical signs suggestive of brucellosis. All cases received medical attention from the district hospital. This achievement in livestock and human health showed that it is possible to control brucellosis in dairy farms, compared to pastoral and agro-pastoral farms, thus providing evidence to adopt these strategies in dairy farms thought to be at risk.

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Sero-Epidemiological Study of Hepatitis E Virus among Thalassemia as High Risk Patients: A Cross-Sectional Survey in Jahrom, Southern, Iran

Global Journal of Health Science ◽

10.5539/gjhs.v8n9p245 ◽

2016 ◽

Vol 8 (9) ◽

pp. 245 ◽

Cited By ~ 5

Author(s):

Abdolreza Sotoodeh Jahromi ◽

Abbas Ahmadi-vasmehjani ◽

Hassan Zabetian ◽

Hossein Hakimelahi ◽

Alireza Yusefi ◽

...

Keyword(s):

Hepatitis E Virus ◽

Age Groups ◽

Serum Levels ◽

Hepatitis E ◽

Cross Sectional Study ◽

Enzyme Linked Immunosorbent Assay ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Significant Difference ◽

Ig G

Hepatitis E virus (HEV) could be cause of viral hepatitis in the developing countries and cause severe epidemics. According to other studies, blood transfusion as a probable route of HEV infection has been suggested. An infection with hepatitis agents such as HEV causes active liver failure in multi-transfusion patients in particular thalassemia. The purpose of this study determines the seropositivity of anti-HEV antibodies in thalassemia individuals in Jahrom. In a cross-sectional study, sera from 110 thalassemia were collected between 2013 and 2014. Enzyme-linked immunosorbent assay (ELISA) method was performed to detection of anti-HEV antibodies. Individuals’ data were collected such as, demographic and clinical, for statistical analysis. Our results show that 10% and 1.8% of the enrolled patients were HEV Ig-G and Ig-M positive antibodies respectively. In addition, there was statiscally significant difference in age groups for prevalence of anti-HEV Ig-G (P = 0.01). Also the serum levels of liver enzymes such as ALT and AST in the HEV Ig-G and Ig-M positive samples were significantly higher than anti-HEV negative samples. But there were no significant difference between sex and splenectomy with anti-HEV positive samples. The results indicate more study are needed to assess HEV screening of blood products to these patients that those have a probably risk of exposure to HEV especially in higher years old.

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