PROTEIN LEVELS IN DEVELOPING AND MATURE PEA SEEDS

1975 ◽  
Vol 55 (1) ◽  
pp. 185-190 ◽  
Author(s):  
S. PANDEY ◽  
E. T. GRITTON
Keyword(s):  
Correlation Coefficient ◽  
Protein Concentration ◽  
Dry Weight ◽  
Protein Determination ◽  
Protein Levels ◽  
Dye Binding ◽  
Percent Protein ◽  
Kjeldahl Method ◽  
Pea Seeds ◽  
Pea Seed

Nine cultivars of peas (Pisum sativum L.) were grown in 1971 and 1972 to study the changes in percent crude protein levels during maturation. Percent protein was found to vary with years, cultivars, and stages of maturity. The year × cultivar, year × maturity, and cultivar × maturity interactions were also important. On a dry weight basis, pea protein concentration as determined by the Kjeldahl method showed a decrease with increasing pea seed maturity through the canning stage. The correlation coefficient between the Kjeldahl and Udy dye-binding methods of protein determination was 0.86 at the mature seed stage, and negligible at the canning stage. There was a highly significant correlation (r = 0.91) between protein concentration at the canning and mature stages of seeds (Kjeldahl method). The correlation coefficient between protein values obtained by the dye-binding method of Ashworth et al. and the Kjeldahl method was lower than between the Kjeldahl and Udy methods.

Measuring Protein in Frozen Dairy Desserts by Dye Binding

1980 ◽  
Vol 43 (10) ◽  
pp. 753-755 ◽  
Author(s):  
J. C. BRUHN ◽  
S. PECORE ◽  
A. A. FRANKE
Keyword(s):  
Statistical Analysis ◽  
Protein Content ◽  
Total Nitrogen ◽  
Protein Concentration ◽  
Protein Nitrogen ◽  
Acid Orange ◽  
Two Samples ◽  
Dye Binding ◽  
Kjeldahl Method ◽  
The Difference

The protein content of 24 samples of vanilla ice cream, ice milk and related frozen dairy desserts was determined by the Kjeldahl method and Acid Orange 12 dye binding method. Statistical analysis showed the correlation between the two methods was highly significant. Excepting two samples, the Kjeldahl protein results based on total nitrogen consistently gave higher protein values than dye binding, the difference averaging +.11%. When the Kjeldahl protein results were corrected for the non-protein nitrogen present, the resulting protein values averaged .20% lower than the dye binding values. These results indicate that the dye binding method is sufficiently accurate for monitoring protein concentration in the frozen dairy desserts studied.

LYSINE SCREENING IN BARLEY WITH A MODIFIED UDY DYE-BINDING METHOD

1975 ◽  
Vol 55 (3) ◽  
pp. 685-689 ◽  
Author(s):  
R. S. BHATTY ◽  
K. K. WU
Keyword(s):  
Amino Acid ◽  
Hordeum Vulgare ◽  
Correlation Coefficient ◽  
Initial Screening ◽  
Protein Determination ◽  
Amino Acid Analyzer ◽  
Dye Binding ◽  
Meal Protein ◽  
Barley Genotypes

A modified Udy method of protein determination and an amino acid analyzer method were compared for lysine screening in a number of lines and cultivars of barley (Hordeum vulgare and H. distichum). The lysine values obtained with the modified Udy procedure gave a correlation coefficient of + 0.96 with the analyzer lysine expressed as grams of lysine per 100 g meal, and a correlation coefficient of + 0.80 when the lysine values were expressed as grams of lysine per 100 g of meal protein. The modified Udy method was satisfactory for initial screening of lysine in barley genotypes.

scholarly journals Cerebrospinal fluid biomarkers of neuroinflammation in children with hydrocephalus and shunt malfunction

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Carolyn A. Harris ◽  
Diego M. Morales ◽  
Rooshan Arshad ◽  
James P. McAllister ◽  
David D. Limbrick
Keyword(s):  
Cerebrospinal Fluid ◽  
Inflammatory Cytokines ◽  
Protein Concentration ◽  
Shunt Revision ◽  
Csf Shunt ◽  
Shunt Failure ◽  
Revision Operation ◽  
Protein Levels ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

Abstract Background Approximately 30% of cerebrospinal fluid (CSF) shunt systems for hydrocephalus fail within the first year and 98% of all patients will have shunt failure in their lifetime. Obstruction remains the most common reason for shunt failure. Previous evidence suggests elevated pro-inflammatory cytokines in CSF are associated with worsening clinical outcomes in neuroinflammatory diseases. The aim of this study was to determine whether cytokines and matrix metalloproteinases (MMPs) contribute towards shunt failure in hydrocephalus. Methods Using multiplex ELISA, this study examined shunt failure through the CSF protein concentration profiles of select pro-inflammatory and anti-inflammatory cytokines, as well as select MMPs. Interdependencies such as the past number of previous revisions, length of time implanted, patient age, and obstruction or non-obstruction revision were examined. The pro-inflammatory cytokines were IL-1β, IL-2, IL-5, IL-6, IL-8, IL-12, IL-17, TNF-α, GM-CSF, IFN-γ. The anti-inflammatory cytokines were IL-4 and IL-10, and the MMPs were MMP-2, MMP-3, MMP-7, MMP-9. Protein concentration is reported as pg/mL for each analyte. Results Patient CSF was obtained at the time of shunt revision operation; all pediatric (< 18), totaling n = 38. IL-10, IL-6, IL-8 and MMP-7 demonstrated significantly increased concentrations in patient CSF for the non-obstructed subgroup. Etiological examination revealed IL-6 was increased in both obstructed and non-obstructed cases for PHH and congenital hydrocephalic patients, while IL-8 was higher only in PHH patients. In terms of number of past revisions, IL-10, IL-6, IL-8, MMP-7 and MMP-9 progressively increased from zero to two past revisions and then remained low for subsequent revisions. This presentation was notably absent in the obstruction subgroup. Shunts implanted for three months or less showed significantly increased concentrations of IL-6, IL-8, and MMP-7 in the obstruction subgroup. Lastly, only patients aged six months or less presented with significantly increased concentration of IL-8 and MMP-7. Conclusion Non-obstructive cases are reported here to accompany significantly higher CSF cytokine and MMP protein levels compared to obstructive cases for IL-10, IL-6, IL-8, MMP-7 and MMP-9. A closer examination of the definition of obstruction and the role neuroinflammation plays in creating shunt obstruction in hydrocephalic patients is suggested.

β-Amyrin Synthase1 Controls the Accumulation of the Major Saponins Present in Pea (Pisum sativum)

2021 ◽  
Author(s):  
Vanessa Vernoud ◽  
Ludivine Lebeigle ◽  
Jocelyn Munier ◽  
Julie Marais ◽  
Myriam Sanchez ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Bitter Taste ◽  
Crop Improvement ◽  
Functional Protein ◽  
Saponin Biosynthesis ◽  
Physiological Quality ◽  
Pea Seeds ◽  
Identification And Characterization ◽  
Pea Seed

Abstract The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart an undesirable bitter taste when used as an ingredient in foodstuffs. In this paper, we describe the identification and characterization of a gene involved in saponin biosynthesis during pea seed development, by screening mutants obtained from two Pisum sativum TILLING (Targeting Induced Local Lesions in Genomes) populations in two different genetic backgrounds. The mutations studied are located in a gene designated PsBAS1 (β-amyrin synthase1) which is highly expressed in maturing pea seeds and which encodes a protein previously shown to correspond to an active β-amyrin synthase. The first allele is a nonsense mutation, while the second mutation is located in a splice site and gives rise to a mis-spliced transcript encoding a truncated, non-functional protein. The homozygous mutant seeds accumulated virtually no saponin without affecting seed nutritional or physiological quality. Interestingly, BAS1 appears to control saponin accumulation in all other tissues of the plant examined. These lines represent a first step in the development of pea varieties lacking bitterness off-flavours in their seeds. Our work also shows that TILLING populations in different genetic backgrounds represent valuable genetic resources for both crop improvement and functional genomics.

Changes in casein composition of goats' milk during the course of lactation: physiological inferences and technological implications

1995 ◽  
Vol 62 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Joanna R. Brown ◽  
Andrew J. R. Law ◽  
Christopher H. Knight
Keyword(s):  
Cation Exchange ◽  
Protein Concentration ◽  
Stage Of Lactation ◽  
Sds Page ◽  
Lower Protein ◽  
Kjeldahl Method ◽  
Compositional Changes ◽  
The Individual ◽  
Cheese Production ◽  
Peak Lactation

SummaryFive British Saanen goats were milk sampled during the first 39 weeks of lactation to determine changes in casein composition. Caseins were separated by anion- and cation-exchange FPLC to determine the relative amounts of the individual caseins. Acid, alkaline and SDS-PAGE were used to determine possible genetic polymorphisms and observe any lactational changes. Total casein nitrogen was determined using a micro-Kjeldahl method and this allowed the concentrations of individual caseins to be calculated. The milk of one animal, which had the deduced genotype αs1-CnAB, showed higher concentrations of both total and αs1-casein. The remainder of the group were either heterozygous αs1-CnBE or, more probably, homozygous αs1-CnE and produced milk of a generally lower protein concentration. Both FPLC and PAGE results showed that the relative amounts and concentrations of αs2-casein decreased with stage of lactation, consistent with its susceptibility to proteolysis. The relative amounts of the breakdown products of plasmin attack on β-casein, γ-caseins, were highly negatively correlated with milk yield (r = –0·942, P < 0·001) in the declining phase of lactation, reflecting the gradual involution of the gland at this time. The relative amount of κ-casein increased by ∼ 50% after peak lactation and its concentration almost doubled near the end of lactation. These compositional changes may alter the processing qualities of goats' milk in relation to cheese production.

SELECTED PHYSIOLOGICAL EFFECTS RESULTING FROM ADDITION OF SODIUM BICARBONATE TO DIETS FOR YEARLING HORSES

1985 ◽  
Vol 65 (3) ◽  
pp. 745-751
Author(s):  
N. R. DEUEL ◽  
L. M. LAWRENCE ◽  
W. W. ALBERT
Keyword(s):  
Sodium Bicarbonate ◽  
Venous Blood ◽  
Average Daily Gain ◽  
Dry Weight ◽  
Serum Glucose ◽  
Feed Additives ◽  
Urine Ph ◽  
Lower Serum ◽  
Mineral Supplement ◽  
Protein Levels

Sodium bicarbonate or a commercial vitamin-mineral mixture was included at the 1% level (air-dry weight) in the diets of 16 yearling horses over a 140-day period in a 2 × 2 factorial design. Feed additives resulted in a pH range from 5.78 to 7.87 in the concentrate portion of the diets. Feces, urine and venous blood were collected 24 h after feeding the concentrate. Average daily gain, respiration rate, venous pCO2 and pO2, as well as serum K, Cl, Ca, P, urea N and protein levels were unaffected by treatment. Horses receiving the vitamin-mineral supplement had higher serum Na and lower serum glucose concentrations than horses not receiving the supplement. Horses receiving sodium bicarbonate had a higher urine pH, lower fecal pH, higher packed cell volume, lower blood pH and lower serum glucose concentrations at 24 h after a meal than horses not receiving the buffer. Key words: Horse, sodium bicarbonate, dietary supplements, hematology

scholarly journals Genotypic Variationin Dry Weight and Nitrogen Concentration of Wheat Plant Parts; Relations to Grain Yield and Grain Protein Concentration

2012 ◽  
Vol 4 (11) ◽  
Author(s):  
Ali Hafeez Malik ◽  
Allan Andersson ◽  
Ramune Kuktaite ◽  
Muhammad Yaqub Mujahid ◽  
Bismillah Khan ◽  
...  
Keyword(s):  
Grain Yield ◽  
Protein Concentration ◽  
Nitrogen Concentration ◽  
Wheat Plant ◽  
Dry Weight ◽  
Grain Protein ◽  
Grain Protein Concentration ◽  
Plant Parts

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Evaluation of airway inflammation in mechanically ventilated patients using cell count and protein concentration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rodopi Stamatiou ◽  
Vasiliki Tsolaki ◽  
Apostolia Hatziefthimiou ◽  
Epaminondas Zakynthinos ◽  
Demosthenes Makris
Keyword(s):  
Airway Inflammation ◽  
Protein Concentration ◽  
Cell Number ◽  
Mechanically Ventilated ◽  
Mechanically Ventilated Patients ◽  
Bronchial Secretions ◽  
Protein Levels ◽  
Tnf Α ◽  
Standardize Sampling ◽  
Ventilated Patients

AbstractMechanically ventilated (MV) patients may present airway inflammation and elevated secretion production. However, it is unknown whether cell and/or protein counts in bronchial samples may be useful to evaluate their clinical condition. Our aim was to standardize sampling and propose a new mechanical mucus dissolution in Tracheal-Bronchial secretions. In all patients, bronchial lining fluid aspiration (BLF), Bronchoalveolar lavage (BAL) and Bronchial Washings (BW40, BW5) were performed, while visible bronchial secretions were obtained via bronchoscopy (VBS) and blinded, via a common catheter for tracheobronchial aspiration (AC). Mucus was mechanically or DTT dissolved and cell number was count. Protein, albumin and TNF-α levels were measured, in mucus dissolved samples from control and MV patients. Cell number and protein levels were elevated in mucus dissolved compared to non-dissolved, or DTT dissolved. Cell number and TNF-α levels were elevated in MV patients compared to controls, while protein levels were lower in MV patients. Differences in cell and protein levels were observed in samples acquired using different sampling technics. Therefore, mechanical mucus dissolution provides a proper sample for evaluation, and the sampling technic used can influence the sample’s characteristics.

scholarly journals The distribution of phospholipase D in developing and mature plants

1969 ◽  
Vol 112 (5) ◽  
pp. 787-794 ◽  
Author(s):  
R. H. Quarles ◽  
R. M. C. Dawson
Keyword(s):  
Phospholipase D ◽  
Dry Weight ◽  
Total Activity ◽  
Weight Basis ◽  
Carrot Root ◽  
Aerial Parts ◽  
Central Stalk ◽  
Pea Seeds ◽  
The Individual ◽  
Germination And Growth

1. The distribution of phospholipase D (phosphatidylcholine phosphatido-hydrolase, EC 3.1.4.4) was examined in the tissues of a number of plants and seeds. 2. The highest activities were found in various swollen storage tissues of certain plants: cabbage, central stalk; cauliflower, flower; celery, swollen leaf stalk; Kohl rabi, swollen stem; carrot, root; pea and marrow, seed. 3. Appreciable activity was retained in pea seeds for at least 1 year after drying. After germination and growth in the dark the total activity present in the cotyledons and also in the whole seedling decreased. 4. In the growing pea seedling (7 days old), about 3% of the total activity was in the plumule, 9% in the root and the remainder in the cotyledons. However, the activity in the root on a dry-weight basis was higher than that in the cotyledons. In both the root and the plumule the activity on a wet- or a dry-weight basis was highest in the growing tip. 5. The activity per dry weight in the roots and aerial parts of pea plants declined to low values as growth continued, but roots struck from cuttings of mature plants showed the same high activity as found in roots from young seedlings with cotyledons attached. 6. The total phospholipids present in the cotyledons of pea seeds were depleted on germination and growth. Of the individual phospholipids, phosphatidylcholine and phosphatidylethanolamine showed the same loss in 11 days as the whole phospholipid fraction, whereas phosphatidylinositol was decreased to a greater extent and cardiolipin and phosphatidylserine were not decreased. There was no increase of phosphatidic acid, as might have been expected if the phospholipids had disappeared through phospholipase D hydrolysis. 7. It is concluded that phospholipase D in plant storage tissues and seeds may be related to the rapid growth involved in their formation rather than being necessary for the utilization of their food reserve substances.

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