Proteolysis in alfalfa silages made from different cultivars

2001 ◽  
Vol 81 (4) ◽  
pp. 685-692 ◽  
Author(s):  
G. F. Tremblay ◽  
G. Bélanger ◽  
K. B. McRae ◽  
R. Michaud

During silage fermentation, proteolysis reduces the nutritional value of N, particularly in alfalfa. This study evaluated the proteolysis of 27 alfalfa cultivars seeded in triplicate in 2 consecutive years. Forage from each plot was wilted to a targeted dry matter (DM) content of 250 g kg–1 and ensiled in laboratory silos. Minisilos were made with spring growth and summer regrowth in the first year of production for the first seeding, and with the first 2 yr of production for the second seeding, giving 3 harvest years altogether. Concentrations of NPN, NH3, FAA, DM, TN, and pH were measured in silages, whereas RUP concentration was evaluated in forages. Low NPN concentration indicates low proteolysis during silage fermentation. Spring growth and summer regrowth were analyzed separately by ANOVA as a completely randomized block design replicated over 3 harvest years. A principal component analysis was then performed on the ANOVA means. Silage DM was used as covariate when it was deemed appropriate. There was a significant variation among cultivars for NPN concentration in silages made of summer regrowth; it varied from 612 to 717 g kg–1 of TN. Concentration of NPN was significantly lower for cultivars WL 225, Rangelander, Iroquois, and WL 222, and higher for cultivars Oneida VR, Arrow, Vernal, Spredor 2, and Armor. Oneida VR and Rangelander had respectively high and low silage NPN concentration in both spring growth and summer regrowth. From cultivars with low silage NPN concentration, Rangelander was the only one with high forage RUP concentration in both spring growth and summer regrowth; proteins in this cultivar would be more resistant to microbial degradation during ensiling and in the rumen. Key words: Non protein nitrogen; proteolysis; alfalfa silage; cultivars

2018 ◽  
Vol 39 (6) ◽  
pp. 2517
Author(s):  
Armindo Neivo Kichel ◽  
Luis Carlos Ferreira de Souza ◽  
Roberto Giolo de Almeida ◽  
José Alexandre Agiova da Costa

This study aimed to assess productivity and nutritional value of the tropical grasses Brachiaria brizantha cv. Piatã, Xaraés, and Marandu, Panicum maximum cv. Mombaça, and B. ruziziensis cv. Kennedy in the interseason of an integrated crop-livestock (ICL) system since alternatives are needed for forage production for animal grazing in Autumn and Winter. The experimental design was a randomized block design in a split-split plot scheme with four replications. The treatments of plots consisted of five grasses, subplots consisted of three cropping systems (monoculture, intercropping with corn and unsuppressed grass, and intercropped with corn and suppressed grass), and sub-subplots consisted of four cutting intervals of grasses (50, 90, 125, and 195 days after emergence - DAE). The experiment was carried out from February to September 2014. Dry matter (DM) productivity, obtained at 195 DAE for the three cropping systems (monoculture grass, unsuppressed and suppressed grass in intercropping), were 18.45, 7.15, and 3.05 t ha?1, respectively, and average crude protein contents of leaf blades of grasses decreased linearly between the cutting intervals of 50 to 195 DAE from 19.95 to 9.70%, respectively. Under integrated systems, the studied grasses showed better yields and nutritional quality when compared to traditional grazing systems. Panicum maximum cv. Mombaça and Brachiaria brizantha cv. Xaraés and Piatã had the highest leaf and crude protein yields when compared to Brachiaria ruziziensis cv. Kennedy and Brachiaria brizantha cv. Marandu. In terms of nutritional value, Brachiaria ruziziensis cv. Kennedy was superior to Mombaça and Xaraés grasses but had lower total dry matter yield. Finally, Xaraés, Piatã and Mombaça grasses are recommended choices ICL systems when fodder grass production is the goal.


Author(s):  
Nyimas Popi Indriani ◽  
Yuyun Yuwariah ◽  
Ana Rochana ◽  
, Iin Susilawati ◽  
Lizah Khairani

The role of Vesicular Arbuscular Mycorrhiza (VAM) and rock phosphate application on production and nutritional value of centro legumes was studied. The aim of the study was to know the interaction of rock phosphate and VAM and to know the optimum dosage of VAM and rock phosphate on production and nutritional value of centro legumes. The experimental design was randomized block design. The treatments and rock phosphate levels was 0, 100, 200 and 300 kg ha-1 of P2O5 with and without VAM. The results revealed that VAM application increase the plant height and dry matter of centro legumes. The optimum dosage of Rock Phosphate was 200 kg ha-1 of P2O5.


2021 ◽  
Vol 42 (6) ◽  
pp. 3463-3484
Author(s):  
Ícaro Daniel Alves dos Santos Souza ◽  
◽  
Vicente Ribeiro Rocha Júnior ◽  
Flávio Pinto Monção ◽  
Rafael Augusto da Costa Parrella ◽  
...  

The objective was to evaluate the structural, yield and nutritional characteristics of biomass sorghum BRS 716 managed in different row planting spacing and harvest age in a semiarid region of Brazil. Three row spacing (45, 70 and 90 cm; plots) and four maturity at harvest (70, 100, 130 and 160 days; subplots) were evaluated following a randomized block design in a 3 x 4 split plot arrangement, with eight blocks. The useful area was 3 x 15 m. Variation in soil fertility in the area was the blocking factor. There was interaction between row spacing and maturity at harvest (P < 0.05) on plant height (P = 0.01), dry matter yield (DMY; P < 0.01) and proportion of senescent material (P = 0.01). The DMY observed at the age of 160 days and spacing of 90 cm was 41.40% higher than the DMY at the same age and in spacings of 45 and 70 cm (mean of 21.45 t/ha). As the maturity at harvest increased, there was a reduction in the crude protein content (P < 0.01), potential degradability (P < 0.01) and effective degradability (P < 0.01) of dry matter, and the content of non-fiber carbohydrates (P < 0.01), total digestible nutrients (P < 0.01) and the readily soluble fraction (P < 0.01) of the dry matter increased. Considering the yield and nutritional characteristics of biomass sorghum BRS 716 managed in the semiarid region for silage production, the row spacing of 90 cm and the maturity at harvest of 160 days after planting are recommended.


2017 ◽  
Vol 47 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Júnior Melo Damian ◽  
Clovis Orlando Da Ros ◽  
Rodrigo Ferreira da Silva ◽  
Ivanir José Coldebella ◽  
Diego Henrique Simon

ABSTRACT Maize and sorghum are the main raw materials in the production of silage for animal feed, with mineral fertilization being worthy of note when the goal is to increase gains in the amount and quality of the forage. This study aimed at evaluating the contribution of N, P or K doses to the dry matter and crude protein yield in maize and sorghum grown for silage. The experiments were carried out in a randomized block design, with four replications, during five successive crops of maize (three summer seasons) and sorghum (two off-season). Five doses of N (0 kg ha-1, 50 kg ha-1, 100 kg ha-1, 150 kg ha-1 and 200 kg ha-1), five doses of P2O5 (0 kg ha-1, 40 kg ha-1, 80 kg ha-1, 120 kg ha-1 and 160 kg ha-1) and five doses of K2O (0 kg ha-1, 30 kg ha-1, 60 kg ha-1, 90 kg ha-1 and 120 kg ha-1) were applied to each crop in the same experimental area. The N doses contributed to an increase in the crude protein yield in the five successive crops of maize and sorghum, together with an increase in dry matter and/or protein concentration. Crude protein increased 59.5-312.9 % for both crops. The soils used for the succession cropping system of maize and sorghum for silage had "very high" levels of P and K. Therefore, the P fertilization had no effect on the dry matter or crude protein yield in the first year of cultivation, similarly to the K fertilization during the five successive crop seasons.


2015 ◽  
Vol 87 (4) ◽  
pp. 2301-2307 ◽  
Author(s):  
MATHEUS G. SILVA ◽  
ORIVALDO ARF ◽  
PAULO E. TEODORO

ABSTRACT The objective of this study was to investigate the effect of interaction between nitrogen topdressing and different application ways (active ingredients) a.i. fluazifop-p-butyl + fomesafen in weed control and agronomic performance of common bean. The experiment was conducted during winter 2003 in Selvíria/MS. The experimental design used was a randomized block design with four replications in a factorial scheme 2x7. The first factor was composed by the absence or presence of nitrogen topdressing, while the second factor consisted of different application ways of fluazifop-p-butyl + fomesafen. The following variables were measured: leaf N content, dry matter of plants, yield components (number of pods plant-1, number of grains plant-1, the average number of grains pod-1 and mass of 100 grains), grain yield, phytotoxicity and weed control percentage. The nitrogen topdressing with 75 kg ha-1provided higher dry matter of plants, higher weed control and higher common bean yield of irrigated winter. In the absence of nitrogen topdressing in the application of urea before or together to fluazifop-p-butyl + fomesafen increased their effectiveness in controlling weeds without interference in the agronomic performance of common bean.


2010 ◽  
Vol 39 (8) ◽  
pp. 1666-1675 ◽  
Author(s):  
Marcio Mahmoud Megda ◽  
Francisco Antonio Monteiro

The objective of this work was to study morphogenic characteristics, and dry matter production of roots and shoots of marandu palisadegrass (Brachiaria brizantha cv. Marandu) submitted to combinations of nitrogen and potassium, in a nutritive solution, employing silica as substrate. The experiment was carried out in a greenhouse during the summer. It was used a 5² fractionated factorial scheme with 13 combinations of nitrogen and potassium, which were distributed in a randomized block design, with four replications. The nitrogen × potassium interaction was significant for the number of tillers and leaves, for leaf area, for shoots and root section dry mass, for total length and surface and specific length and surface in the roots. Production of aerial part dry mass positively correlated with the number of tillers and leaves and grass leaf area. Nitrogen rates modulated the root system development, and the root specific length and surface decreased when high rates of nitrogen and potassium were supllied. Nitrogen and potassium influence Marandu palisadegrass morphogenic characteristics, which are determinant for grass dry matter production.


CERNE ◽  
2017 ◽  
Vol 23 (4) ◽  
pp. 507-515 ◽  
Author(s):  
Veronica Brito da Silva ◽  
Rogerio Figueiredo Daher ◽  
Bruna Rafaela da Silva Menezes ◽  
Maria Lorraine Fonseca Oliveira ◽  
Maria do Socorro Bezerra Araújo ◽  
...  

ABSTRACT The elephant-grass undergoes successive cutting and periodical evaluations that it possible to identify clones with high stability for dry matter production, which can be used for energy production. The present study was carried out to evaluate stability dry matter yield for different parametric and non-parametric methods in elephant grass genotypes for biomass production undergoes successive cutting in the agroclimatic conditions of the Norte Fluminense (RJ, Brazil). The variable measured in the 40 genotypes was dry matter yield (DMY) at 2009, 2010 and 2011 in a field study designed as randomized block design with two replicates. Each sample was grown in different environment condition. The stability methods tested were the Yates and Cochran’s, Plaisted and Peterson’s, Wricke’s ecovalence, Annicchiarico’s, Lin and Binns’ and Kang and Phan’s. Results indicated that cutting (E) and genotypes (G) influenced significantly on the performance of dry matter yield. The non-parametric stability methods were effective for the evaluation of stability in dry matter yield. Genotypes Mercker, Pinda-México, Mercker 86-México, Guaçu/IZ, Mercker Pinda, P-241-Piracicaba and Cubano Pinda were stable stability dry matter yield. Hence, there are genotypes may be exploited in future breeding programmes in order to improve productivity of upland elephant grass over environment.


1985 ◽  
Vol 36 (3) ◽  
pp. 483 ◽  
Author(s):  
JK Egan ◽  
PT Doyle

Six mature Merino sheep received three treatments in a randomized block design experiment. The treatments were: chopped oaten hay diet at 90% of ad libitum intake without urea (L); the ciet offered at the same level as for L with urea infused into the rumen at 11.5 g kg-1 dry matter intake (LU); and the diet offered at 90% of the ad libitum intake achieved with urea infused at 11.5 g kg-1 dry matter intake (HU). Sheep given HU consumed 37% more (P < 0.01) organic matter (OM) than those fed L or LU, but the apparent digestibility of OM did not vary (59.2-61.8%) between treatments. The addit onal food consumption was associated with c. 20% increase (P < 0.05) in the weight of OM in the reticulorumen and significantly higher (by 10-35%; P < 0.05) fractional outflow rates of most dietary and microbial constituents of digesta. The fractional digestion rate of potentially digestible plant cell walls was not affected by urea, but the flow of microbial non-ammonia nitrogen from the abomasum was enhanced (L, 7.0; LU, 8.2; HU, 12.5 g day-1; P < 0.05). The results of this study suggest that the stimulatory effect of urea upon food intake was associated with the provision of additional microbial protein for digestion in the intestines, rather than changes in the rate or extent of organic matter fermentation in the reticulorumen.


2009 ◽  
Vol 38 (9) ◽  
pp. 1757-1761
Author(s):  
José Vieira Neto ◽  
Vinícius de Souza Cantarelli ◽  
Elias Tadeu Fialho ◽  
Márcio Gilberto Zangeronimo ◽  
Nikolas de Oliveira Amaral ◽  
...  

The objective of this study was to evaluate different textures and storage forms of corn in piglet diets. Two experiments were conducted, one of which tested digestibility of nutrients and the other diet performance. In the digestibility trial, 24 crossbred (Landrace X Large White) barrows with an initial weight of 18.7 ± 1.5 kg were used. They were kept in metabolic cages in a randomized block design using the factorial arrangement 2 X 2 (type of corn - dent and flint X type of storage - moist grain silage and dry grain) and six replicates, with one animal as the experimental unit. The values of metabolizable energy (ME) obtained were 3841 kcal of ME/kg of dry matter (DM) from dry corn, 3912 kcal of ME/kg of DM from moist corn, 4022 kcal of DM from ensiled dry corn and 3928 kcal of ME/kg of DM from ensiled humidity corn. The ensilage process increases the digestibility coefficient of dry matter and crude protein and increases the digestible energy of the diets, independent of the process form. The type of corn did not influence these variables. In the performance trial, 60 barrows (initial weight of 6.5 ± 1.5 kg) of the same stock were used during 28 days of post-weaning distributed in the same experimental design, with five replicates and three animals as the experimental unit. The treatments did not influence the daily weigh gain or the daily feed intake. The dentate corn and the ensilage process decreased the feed conversion of the independent form (no significant interaction). The substitution of dry for ensilage corn increased the digestibility of the nutrients in the diets and the feed conversion of the piglets from 7 to 15 kg. Dentate corn promotes best feed conversion in this phase.


2013 ◽  
Vol 48 (4) ◽  
pp. 411-416 ◽  
Author(s):  
Cecília Khusala Verardi ◽  
Erivaldo José Scaloppi Junior ◽  
Guilherme Augusto Peres Silva ◽  
Lígia Regina Lima Gouvêa ◽  
Paulo de Souza Gonçalves

The objective of this work was to assess the genetic parameters and to estimate genetic gains in young rubber tree progenies. The experiments were carried out during three years, in a randomized block design, with six replicates and ten plants per plot, in three representative Hevea crop regions of the state of São Paulo, Brazil. Twenty-two progenies were evaluated, from three to five years old, for rubber yield and annual girth growth. Genetic gain was estimated with the multi-effect index (MEI). Selection by progenies means provided greater estimated genetic gain than selection based on individuals, since heritability values of progeny means were greater than the ones of individual heritability, for both evaluated variables, in all the assessment years. The selection of the three best progenies for rubber yield provided a selection gain of 1.28 g per plant. The genetic gains estimated with MEI using data from early assessments (from 3 to 5-year-old) were generally high for annual girth growth and rubber yield. The high genetic gains for annual girth growth in the first year of assessment indicate that progenies can be selected at the beginning of the breeding program. Population effective size was consistent with the three progenies selected, showing that they were not related and that the population genetic variability is ensured. Early selection with the genetic gains estimated by MEI can be made on rubber tree progenies.


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