Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

ABSTRACT: Evaluation of differential candidate gene expression in contrasting soybean seeds is an auxiliary tool in the partial elucidation of processes involved in seeds formation, as well as it contributes to the generation of new information that can be used in future research or in the development of r genetic superior constitutions. The aim of this study was to evaluate the expression of two candidate genes, SBP and leginsulin genes, possibly involved in seed quality, in contrasting coats of four soybean genotypes. Two cultivars of yellow soybeans were used, BMX Potência RR and CD 202, and two lines of black soybean, TP and IAC. Gene expression was evaluated using qPCR in seven stages of development from seed coats for four genotypes, at 25, 30, 35, 40, 45, 50, and 55 days after anthesis. The design was completely randomized, with three replications. Data were subjected to analysis of variance and means compared by Tukey's test at 5% probability. SBP and leginsulin gene have higher expression in the early phases of development from seed coats of BMX Potência RR cultivar, followed by the IAC line. These genotypes are therefore of interest for further research involving these genes.

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Early Weaning Affects Liver Antioxidant Function in Piglets

Animals ◽

10.3390/ani11092679 ◽

2021 ◽

Vol 11 (9) ◽

pp. 2679

Author(s):

Lihuai Yu ◽

Hongmin Li ◽

Zhong Peng ◽

Yuzhu Ge ◽

Jun Liu ◽

...

Keyword(s):

Gene Expression ◽

Body Weight ◽

Antioxidant Enzymes ◽

Liver Weight ◽

The Body ◽

Early Weaning ◽

Large White ◽

Enzymes Activity ◽

Antioxidant Function ◽

The Impact

This study examined the impact of early weaning on antioxidant function in piglets. A total of 40 Duroc × Landrace × Large White, 21-day-old piglets (half male and half female) were divided into suckling groups (SG) and weaning groups (WG). Piglets in WG were weaned at the 21st day, while the piglets in SG continued to get breastfed. Eight piglets from each group were randomly selected and slaughtered at 24th-day (SG3, WG3) and 28th-day old (SG7, WG7). The body weight, liver index, hepatocyte morphology, antioxidant enzymes activity, gene expression of antioxidant enzymes, and Nrf2 signaling in the liver of piglets were measured. The results showed that weaning caused decreased body weight (p < 0.01), lower liver weight (p < 0.01), and decreased the liver organ index (p < 0.05) of piglets. The area and size of hepatocytes in the WG group was smaller than that in the SG group (p < 0.05). We also observed that weaning reduced the activity of superoxide dismutase (SOD) and catalase (CAT) (p < 0.05) in the liver of piglets. Relative to the SG3 group, the gene expression of GSH-Px in liver of WG3 was significantly reduced (p < 0.05). The gene expression of Nrf2 in the SG3 group was higher than that in the WG3 group (p < 0.01). The gene expression of NQO1 in the SG7 group was higher than that in the WG7 group (p < 0.05). In conclusion, weaning resulted in lower weight, slowed liver development, and reduced antioxidant enzymes activity, thereby impairing liver antioxidant function and suppressing piglet growth.

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The Effect of 8 Weeks of Resistance Training on Gene Expression Lymphocyte Antioxidant Enzymes and Malondialdehyde in Healthy Inactive Men

Qom Univ Med Sci J ◽

10.29252/qums.12.2.74 ◽

2018 ◽

Vol 12 (2) ◽

pp. 74-83

Author(s):

Hamid Amini ◽

Mohammad Ali Azarbayjani ◽

Kamal Azizbeigi Boukani ◽

◽

...

Keyword(s):

Gene Expression ◽

Antioxidant Enzymes ◽

Resistance Training

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Pro-oxidant activity of aluminum in the rat hippocampus: gene expression of antioxidant enzymes after melatonin administration

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2004.10.009 ◽

2005 ◽

Vol 38 (1) ◽

pp. 104-111 ◽

Cited By ~ 65

Author(s):

Mercedes Gómez ◽

José L. Esparza ◽

M. Rosa Nogués ◽

Montserrat Giralt ◽

Maria Cabré ◽

...

Keyword(s):

Gene Expression ◽

Antioxidant Enzymes ◽

Rat Hippocampus ◽

Melatonin Administration

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Fruit maturation stage on the physiological quality of maroon cucumber seeds

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632019v4953188 ◽

2019 ◽

Vol 49 ◽

Author(s):

Cleisson Dener da Silva ◽

Andréia Márcia Santos de Souza David ◽

Josiane Cantuária Figueiredo ◽

Jorge Luiz Rodrigues Barbosa ◽

Rayane Aguiar Alves

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Accelerated Aging ◽

Dry Mass ◽

Maturation Stage ◽

Physiological Maturity ◽

Physiological Quality ◽

Photography Analysis ◽

The Ideal

ABSTRACT To obtain seeds of high physiological quality, it is of paramount importance to define the ideal harvest moment, which oftentimes corresponds to the period when the physiological maturity is reached. This experiment aimed to study the maturation process and determine the best harvest season of maroon cucumber fruits (Liso Gibão cultivar), in order to reach the maximum physiological seed quality. The flowers were labeled during the anthesis and the fruits harvested at 14, 21, 28, 35, 42, 49 and 56 days after the anthesis (DAA). The fruits had their visual aspects registered by photography analysis and, later, the seeds were extracted for physical (water and dry matter contents) and physiological (germination, seedling emergence, emergence speed index, accelerated aging and electrical conductivity) analyses. It was observed that the seeds reach the maximum dry mass (8.08 mg seed-1) between 47 and 49 DAA. However, the seed physiological maturity, characterized by a maximum germination (70 %) and vigor, occurs at 56 DDA. The best season to harvest seeds with a higher physiological quality takes place from 49 to 56 DDA, when the fruits present a yellowish color.

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Muscle Antioxidant Enzymes Activity and Gene Expression Are Altered by Diet-Induced Increase in Muscle Essential Fatty Acid (α-linolenic acid) Concentration in Sheep Used as a Model

Nutrients ◽

10.3390/nu11040723 ◽

2019 ◽

Vol 11 (4) ◽

pp. 723 ◽

Cited By ~ 3

Author(s):

Eric Ponnampalam ◽

Vahid Vahedi ◽

Khageswor Giri ◽

Paul Lewandowski ◽

Joe Jacobs ◽

...

Keyword(s):

Gene Expression ◽

Antioxidant Enzymes ◽

Fatty Acid ◽

Mrna Expression ◽

Linolenic Acid ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities ◽

Post Mortem ◽

Annual Ryegrass ◽

Alpha Linolenic Acid

This study investigated the effect of dietary manipulations on muscle fatty acid composition, the activities and relative mRNA expressions of antioxidant enzymes and the relationship between muscle enzyme activity or mRNA expression and alpha linolenic acid (ALA) concentration in sheep. Eighty-four lambs blocked on liveweight were randomly allocated to four dietary treatments, lucerne pasture (Lucerne), annual ryegrass pasture (Ryegrass), feedlot pellets (Feedlot) or annual ryegrass plus feedlot pellets (RyeFeedlot). After six weeks of feeding, lambs were slaughtered and within 30 min post-mortem, samples collected from the longissimus lumborum (LL) muscle for RNA isolation and measurement of antioxidant enzyme activities. At 24 h post-mortem, LL samples were collected for determination of fatty acid concentrations. Feedlot treatment decreased ALA, eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA) concentrations compared with other treatments and increased linoleic acid (LA) and arachidonic acid (AA) compared with Lucerne and Ryegrass (p < 0.001). The activity of Glutathione peroxidase (GPX1, p < 0.001) and Superoxide dismutase (SOD2, p < 0.001) enzymes in the muscle increased with Lucerne compared to other treatments. Lucerne increased muscle gpx1 mRNA expression by 1.74-fold (p = 0.01) and 1.68-fold (p = 0.05) compared with Feedlot and other diets, respectively. The GPX1 (r2 = 0.319, p = 0.002) and SOD2 (r2 = 0.244, p = 0.009) enzyme activities were positively related to ALA. There was a positive linear relationship between muscle gpx1 (r2 = 0.102, p = 0.017) or sod2 (r2 = 0.049, p = 0.09) mRNA expressions and ALA concentration. This study demonstrates that diet can affect concentrations of ALA and other fatty acids as well as change activities and gene expression of antioxidant enzymes in muscle. Increased antioxidant activity may, in turn, have beneficial effects on the performance, health and wellbeing of animals and humans.

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