scholarly journals The Side Effect of the Melastoma malabathricum L Ethanol Extract on the Gonad Maturation of Female Orange Mud Crab (Scylla olivacea)

2018 ◽  
Vol 25 (4) ◽  
pp. 188
Author(s):  
Nuril Farizah ◽  
Muhammad Zairin JR ◽  
Latifah Kosim Darusman ◽  
Arief Boediono ◽  
Muhammad Agus Suprayudi

The medicinal plants in aquaculture are currently being studied quite extensively by researchers. Medicinal plants can act as an inducer or aphrodisiac and an antifertility agent in gonad maturation. The aimed of the experiment was to known side effects of the herb M. malabathricum L. on maturation process in female mud crab (S. olivacea). This study used 40 crabs with an average weight of 200-250g. The experiment consists of two treatment groups. The controls group (A) that received 100 μl of aquadest and group treatment (B) received 100 μl of 2 mg/g of ethanol extract M. malabathricum L. were studied. For 5, 10, 15, and 20 days of experiment, crabs were anesthetized and sacrified. The ovary was collect for GSI and histology. The end of experiment days (20 day), the hemolymph was collect to used ELISA assay. The result showed, significant differences in treatment group and control groups (p<0.05) in terms the macroscopic morphology of ovary, the mean GSI and the diameter of oocytes. Histological showed the influence of extract M. malabathricum L. on inhibited the ovary development compared with the control groups. Based on this results, it is concluded that ethanol extract of M. malabathricum L. leaves at a dose of 2 mg/g act as an antifertility agent for ovary maturation of S. olivacea.

2015 ◽  
Vol 19 ◽  
pp. 65-70
Author(s):  
CT Samagoro ◽  
A Agunu ◽  
H Nuhu ◽  
Z Mohammed ◽  
EM Abdurrahman ◽  
...  

Traditional contraceptive practices and use of medicinal plants is a common occurrence in Kaduna State, Nigeria. Lawsonia inermis L. (Lythraceae) roots which is the most frequently used anti-fertility agent was evaluated in rats. Apparently healthy female and male Wistar rats were used. Pre-implantation and mating ratio, determinations of implantation sites and Corpora lutea were also conducted. The effects of extract on weight of the rats were also determined. There were loss of implantation sites and decrease in body weight. The number of implantation sites showed dose–response relationship significantly (p£0.05) among the doses of extract and to standard drug (ethinyl estradiol). There was also significant (p£0.05) difference observed in the number of Corpora lutea in all experimental and control groups. Similarly, there was significant (p£0.05) difference observed in all the experimental and control groups on percentage pre-implantation loss. The plant extract exhibit anti-fertility effects. The finding may explain the traditional use of the plant as antifertility agent.Keywords: Traditional Medicine, Contraception, Methanol Extract, L. inermis Root, Medicinal Plants


1982 ◽  
Vol 50 (3_suppl) ◽  
pp. 1151-1159 ◽  
Author(s):  
James H. Hilkey ◽  
Cynthia L. Wilhelm ◽  
Arthur M. Horne

This study was conducted to measure the effects of pretherapy training on selected process and outcome variables in time-limited psychotherapy. 90 inmates in a medium-security Federal Penitentiary who volunteered for the study were divided into experimental and control groups; the experimental subjects were exposed to a videotape presentation of a model psychotherapy group followed by a guided performance experience. Expert group psychotherapists conducted 8 sessions in consecutive weeks. Levels of anxiety and subjects' perceptions of therapy were assessed prior to entering the groups. Quality of group interactions were measured at intervals during the 8 weeks of the study. Attendance and clients', peers', and therapists' perception of progress were assessed as outcome variables. Pre-trained clients went into the therapeutic experience with clearer expectations of group treatment, demonstrated more desirable behaviors in the early stages of therapy and made more progress toward individual goals as perceived by both therapists and peers. Implications for pretraining for overtly resistant clients are discussed.


2020 ◽  
Vol 10 (6) ◽  
pp. 7061-7069

Lansau is a traditional herb medicine from Muna ethnic, Southeast Sulawesi, Indonesia, consisting of 44 medicinal plants. This study was performed to determine and evaluate the antihyperlipidemic effect of ethanol extract of Lansau in high cholesterol and propylthiouracil induced hyperlipidemic rats. The animal test Wistar strain were used and divided into six groups namely KN (Na. CMC 1%), K (-) (Na CMC 1% + MDLT), K (+) (simvastatin 0.40 mg/kg BW), LS I (Lansau ethanol extract 6.907 mg/kg BW), LS II (ethanol extract Lansau 13.814 mg/kg BW) and LS III (Lansau LS III ethanol extract 27.628 mg/kg BW. The results showed that ethanol extract of traditional Lansau medicine with dose LS I 6.907 mg/kg BW, LS II 27.628 mg/kg BW and LS III 27.628 mg/kg BW have significant activity as antihyperlipidemic (cholesterol and triglyceride) compared to negative control groups (p <0.05) and showed the same activity with simvastatin in lowering total cholesterol and triglyceride levels (p> 0.05), which shows its potential as antihyperlipidemic herbal medicine.


2020 ◽  
pp. 61-65
Author(s):  
И.Ю. Постраш ◽  
И.В. Сучкова ◽  
Е.Г. Скворцова ◽  
О.В. Филинская ◽  
А.В. Мостофина

Изучение влияния пробиотического препарата ЭМ-курунга на приросты, анализы крови и помёта птиц проведено на примере цыплят техасских белых перепелов. В качестве объекта исследований использовались цыплята перепелов, начиная с суточного возраста. Средняя масса цыплят на начало эксперимента составила 9,2±0,12 г. Цыплят забивали в возрасте 90 суток. Установлено, что введение в рацион цыплят пробиотического препарата ЭМ-курунга в количестве 0,2 мл на 1 кг живого веса увеличивает сохранность молодняка на 14,8% и приросты птенцов – на 10%. Сравнительный анализ биохимических данных опытной и контрольной групп птицы показал, что введение в рацион опытной группы пробиотика практически не повлияло на белковый обмен. Общий белок у опытной птицы был недостоверно меньше, по сравнению с контролем, на 1,25%, та же картина наблюдалась по уровню альбуминов – меньше на 1,9%, глобулинов – на 1%. Сравнительный анализ показателя кислотности кала птиц опытной и контрольной групп показал достоверное различие. Так, у контрольной группы рН кала имел среднее значение 6,5, у перепелов опытной группы – 7,5. Таким образом, у опытной птицы, в сравнении с контрольной, активная кислотность кишечного содержимого достоверно сдвинулась в слабощелочную сторону, что свидетельствует о благоприятном воздействии пробиотика на микрофлору желудочно-кишечного тракта перепелов, снижая уровень патогенной микрофлоры, активно развивающейся в кислой среде. The study of the effect of the probiotic preparation EM-Kurunga on gains, blood tests and poultry litter was carried out using the example of White Texas quail chickens. Quail chickens starting at daily age were used as the subject of research. The average weight of chickens at the beginning of the experiment was 9.2±0.12 g. Chickens were slaughtered at the age of 90 days. It was established that the introduction of the probiotic preparation EM-Kurunga into the diet of chickens in the amount of 0.2 ml per 1 kg of live weight increases the safety of young animals by 14.8% and the gains of poults – by 10%. A comparative analysis of the biochemical data of the experimental and control groups of poultry showed that the introduction of the probiotic into the diet of the experimental group had practically no effect on protein metabolism. The total protein in the experimental poultry was insignificantly less than in the control by 1.25%, the same pattern was observed in the level of albumin – less by 1.9%, globulins – by 1%. Comparative analysis of the acidity index of poultry feces from the experimental and control groups showed a significant difference. So, in the control group the pH of feces had an average value of 6.5, in the quails of the experimental group – 7.5. Thus, in the experimental poultry in comparison with the control one the active acidity of the intestinal contents significantly shifted to the slightly alkaline side, which indicates a favorable effect of the probiotic on the microbiota of the quail gastrointestinal tract, reducing the level of pathogenic microflora actively developing in an acidic condition.


2021 ◽  
Vol 1 (Volume 1 No 1) ◽  
pp. 38-48
Author(s):  
Hillda Herawati ◽  
Fahrauk Paramayudh ◽  
Rudi Satria Darwis ◽  
Sarah Syarifah

The optimal achievement of orthodontic treatment is determined by a bone remodelling process involving osteoblast, osteoclasts, and the estrogen hormone. Estrogen deficiency can increase osteoclast age and decrease osteoblast, resulting in an imbalance between osteoclasts and osteoblasts. One natural alternative that can replace the role of the hormone estrogen is phytoestrogens. Sauropus androgynus (L.) Merr (katuk) is a phytoestrogen that contains isoflavones with many similarities with estrogens. This research aims to determine the effectiveness of the various doses of ethanol extract of katuk leaves orally on the number of osteoblasts and osteoclasts. This research was conducted using experimental laboratory methods using 24 female Guinea pigs divided into a control group and three groups with various doses of 39.15 mg/BW, 78.3 mg/BW, and 156.5 mg/BW. The observations made in this test were the number of osteoblasts and osteoclasts on the alveolar guinea pig on day 14 and analysed using the one way ANOVA test (p <0.05). All guinea pigs have applied a rubber separator to the left incisor and given a dose according to the group, and after 14th days, histological preparations were made. The results showed that the highest number of osteoblasts was at a dose of 78.3 mg/BW, and the lowest number of osteoclasts was at a dose of 39.15 mg/BW with values of 15.03 ± 2.27 and 1.73 ± 0.56, respectively. Statistically, the number of osteoblasts between the treatment and control groups significantly differed (p = 0.04), while the number of osteoclasts between the treatment and control groups had no significant difference (p = 0.228). This study concludes that katuk leaves extract has effectiveness in increasing the number of osteoblasts in orthodontic treatment, while the decrease in osteoclasts is not statistically proven.


2021 ◽  
pp. 2163-2168
Author(s):  
Saad Thabit Jassim Alrawi ◽  
A. O. Humide ◽  
Mohanad D. Al- jeboori

     This study aimed to determine the effects of alcoholic and aqueous extracts of caper (Capparis Spinosa) and acetic acid on serum lipid profile and proteins levels in mice.  Sixty adult mice with an average weight of 24±4 g grams were divided into four groups (15 mice for each). The first group (G1) was administrated daily with an oral dose of caper alcoholic extract (200 mg/kg) for 28 days. The second group (G2) was administrated daily with an oral dose of caper aqueous extract (200 mg/kg) for 28 days. The third group (G3) was administrated with a daily dose of 10 % acetic acid  (2 ml/kg) for 28 days. The fourth Group (G4) was administrated daily with distilled water for 28 days, as a control group. The levels of lipid profile parameters, blood urea, total protein, albumin, and globulin were determined. The results showed a significant reduction (P≤0.05) in cholesterol and triglyceride levels in mice that were treated with alcohol or aqueous extracts of caper compared with acetic acid-treated and control groups. On the other hands, the results showed a significant reduction (P≤0.05) of blood urea levels in mice that were treated with alcohol or aqueous extracts of caper compared with acetic acid-treated and control groups. While the results recorded non-significant differences in the levels of total protein, albumin, and globulin in the serum of mice of different treatment groups. From the results, it can be concluded that caper has protective effects via acting to improve the lipid profile and urea level in the blood of mice.


1972 ◽  
Vol 136 (5) ◽  
pp. 1031-1042 ◽  
Author(s):  
Chi-Jen Lee ◽  
Rene Dubos

The effects of neonatal infection, perinatal malnutrition, and crowding on the metabolism of brain catecholamine were studied in specific pathogen-free mice. Metabolic turnover of catecholamine was determined by measuring the incorporation of precursor tyrosine-14C into brain tissue, catabolic activity of norepinephrine-3H at various times after intracisternal injection, and tissue levels of dopamine and norepinephrine. The rate of tyrosine incorporation was decreased by neonatal infection but was increased by perinatal malnutrition and crowding. There was no difference in catabolic activity of norepinephrine between infected, crowded, and control groups. In the malnourished group, however, the total radioactivity from norepinephrine was significantly higher than in the control group ½ and 2 hr after injection. The brain contents of dopamine and norepinephrine were depressed in the malnourished group. There was no significant difference in catecholamine levels between infected, crowded, and control groups. In the malnourished group, treatment of the mothers with growth hormone prevented almost completely weight loss during lactation, and also resulted in higher fetal weight. Hormone treatment restored to normal the levels of brain catecholamine and the enzymatic activity of brain tyrosine hydroxylase in progeny of malnourished mothers.


2021 ◽  
Vol 2 (1) ◽  
pp. 41-48
Author(s):  
Suaebah ◽  
Kammisari Dewi ◽  
Ayu Rafiony

Thin nutrition is an acute nutritional problem, as a result of events that occur in a short time such as lack of food intake. The high prevalence of thin underweight nutrition in infants is influenced by three main factors namely poor quality of quantity pangan food consumption as a result of low family food security, poor patterns of foster care and low access to health facilities. The impact of underweight nutrition in infants can reduce intelligence, productivity, creativity, and very influential on the quality of HR. This type of research is a Quasi-Experiment with the design used is pretest-posttest design with control group design. The number of samples for each group of 28 people was taken by purposive sampling technique. Data analysis used parametric statistical tests and non-parametric tests. The results of the analysis of differences in knowledge before and after nutrition education in the two treatment groups there was no significant difference (p = 0.271). For weight gain in children under five given intervention that is the average weight of the pre-test 9.91kg and the average weight of the post-test was 10.13kg with the results of statistical tests showed that there was a significant weight gain (p = 0, 00). The conclusion of this study is that there is no difference between the treatment and control groups for the knowledge of mothers under five and there are differences in the treatment and control groups for toddler weight gain. Parents of toddlers are expected to have more access to information about health through various mass media such as newspapers, magazines, radio and television. In addition, it is also important to get a lot of information by participating in empowering organizations


2020 ◽  
Vol 11 (1) ◽  
pp. 15-19
Author(s):  
Awaludin ◽  
Nuril Fahrizah ◽  
Heppi Iromo ◽  
Muhammad

Karamunting is a plant that contains cholesterol. Cholesterol is needed by mud crabs as precursors of reproductive hormones in ovarian development. This study aims to determine the effect of karamunting leaf extract which is injected with mangrove crabs (Scylla sp) on the level of ovarian maturity. The stages of the research included extraction of karamunting leaves using ethanol 70%, experiments by injecting the parent crabs consisting of 3 treatments A (control), B treatment (0.25 mg / g body weight), C treatment (0.5 mg / g body weight) maintained for 20 days by feeding trash fish and measuring parameters including morphological ovarian development, somatic gonad index (GSI), and hepatosomatic index (HSI). The results showed that by giving karamunting extract at the end of the GSI study on control (1.70%), treatment A (5.61%), treatment B (4.24%) while on ovarian development with morphological observation at the end of the study on treatment ovarian control develops in the TKG II phase, A treatment in the TKG V phase while treatment B in TKG IV. This shows that by giving karamunting extract can provide the development of mud crab ovaries.


2020 ◽  
Vol 13 (1) ◽  
pp. 25-31
Author(s):  
Ari Nurwijayanto ◽  
Mohammad Na’iem ◽  
Atus Syahbudin ◽  
Subagus Wahyuono

ABSTRACT Mount Merapi National Park (MMNP) has known for its high biodiversity. However, exploration related to the potential for antioxidant activity of the medicinal plants in this region is still very limited. Therefore, the aim of this research was to explore of medicinal plants from MMNP and to evaluate the antioxidant activity of the plants. The research began with the collection and identification the medicinal plants to determine the scientific names. Then, plants that have been identified, are dried, mashed and macerated with ethanol (96%). The ethanol extract were evaluated for antioxidant activity using 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) to determine the IC50 value and compared with positive control (ascorbic acid). The results of this exploration were obtained 50 plant species which have been identified its scientific names and classified into 16 families. The largest distribution of plant species was belongs to the family Poaceae, followed by Asteraceae and Rubiaceae. Antioxidant activity assay of the medicinal  plant resulted three plant species with IC50 values ​​less then 6 ppm, i.e. Melastoma malabathricum, Phyllanthus urinaria, and Clidemia hirta. The alkaloid compound detected in M. malabathricum expected to be a potential antioxidant compound. This information can be used as a basic data for developing alternative sources of antioxidants and as an new drug raw materials. Keywords: medicinal plants, Merapi Volcano National Park, antioxidants, DPPH   ABSTRAK Taman Nasional Gunung Merapi (TNGM) telah lama dikenal dengan keanekaragaman hayatinya yang tinggi. Akan tetapi, eksplorasi terkait potensi aktivitas antioksidan pada koleksi tumbuhan obat di wilayah tersebut masih sangat terbatas. Fakta tersebut yang mendasari tujuan dari penelitian ini, yaitu eksplorasi potensi aktivitas antioksidan tumbuhan yang berasal dari TNGM. Penelitian ini diawali dengan pengumpulan dan identifikasi nama ilmiah tumbuhan obat yang tumbuh di wilayah tersebut. Tumbuhan yang telah teridentifikasi kemudian dikeringkan, dihaluskan dan dimaserasi dengan etanol (96%). Ekstrak etanol yang diperoleh kemudian diuji aktivitas antioksidannya melalui penentuan penangkapan radikal bebas menggunakan 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) dengan menentukan nilai IC50-nya dan dibandingkan dengan kontrol positif (asam askorbat). Hasil dari kegiatan eksplorasi ini diperoleh 50 spesies tumbuhan yang telah berhasil diidentifikasi nama ilmiahnya dan digolongkan menjadi 16 famili. Distribusi spesies tumbuhan terbesar termasuk dalam famili Poaceae, diikuti Asteraceae dan Rubiaceae. Pengujian aktivitas antioksidan pada 50 sampel tumbuhan diperoleh tiga spesies tumbuhan dengan nilai IC50 kurang dari 6 ppm, yaitu Melastoma malabathricum, Phyllanthus urinaria, dan Clidemia hirta. Kandungan alkaloid yang terdeteksi pada M. malabathricum, diduga sebagai senyawa antioksidan yang sangat potensial. Informasi tersebut dapat digunakan sebagai dasar pengembangan sumber antioksidan alternatif untuk inovasi bahan baku obat baru.  


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