Antihyperlipidemic Effect of Ethanol Extract of Lansau a Traditional Medicine of Muna Ethnic, Indonesia

Lansau is a traditional herb medicine from Muna ethnic, Southeast Sulawesi, Indonesia, consisting of 44 medicinal plants. This study was performed to determine and evaluate the antihyperlipidemic effect of ethanol extract of Lansau in high cholesterol and propylthiouracil induced hyperlipidemic rats. The animal test Wistar strain were used and divided into six groups namely KN (Na. CMC 1%), K (-) (Na CMC 1% + MDLT), K (+) (simvastatin 0.40 mg/kg BW), LS I (Lansau ethanol extract 6.907 mg/kg BW), LS II (ethanol extract Lansau 13.814 mg/kg BW) and LS III (Lansau LS III ethanol extract 27.628 mg/kg BW. The results showed that ethanol extract of traditional Lansau medicine with dose LS I 6.907 mg/kg BW, LS II 27.628 mg/kg BW and LS III 27.628 mg/kg BW have significant activity as antihyperlipidemic (cholesterol and triglyceride) compared to negative control groups (p <0.05) and showed the same activity with simvastatin in lowering total cholesterol and triglyceride levels (p> 0.05), which shows its potential as antihyperlipidemic herbal medicine.

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UJI EFEK EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava L) TERHADAP KADAR KOLESTEROL TOTAL TIKUS WISTAR (Rattus norvegicus)

Jurnal e-Biomedik ◽

10.35790/ebm.1.1.2013.4565 ◽

2013 ◽

Vol 1 (1) ◽

Author(s):

Irianto Girik Allo ◽

Pemsi Mona Wowor ◽

Henoch Awaloei

Keyword(s):

Total Cholesterol ◽

Ethanol Extract ◽

Negative Control Group ◽

Psidium Guajava ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Cholesterol Levels ◽

Guava Leaves ◽

Guava Leaf

Abstract: This research aims to determine the effect of ethanol extract of guava leaves on levels of total cholesterol of rat that is induced propylthiouracil. This was an experimental research. The sample used was 15 rats were divided into 5 groups (1 group is consist of 3 rats), namely: 1) positive control, 2) extract dose of 200 mg/kgBB, 3) extract dose of 400 mg/kgBB, 4) extract dose of 800 mg/kgBB and 5) negative control. Group 1 to 4 are given propylthiouracil given for 14 days, then given treatment appropiate with the group. The result show that: 1) the average of total cholesterol levels increased after administration propylthiouracil from 156.34 mg/dL to 247.84 mg/dL, 2) at a dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB showed a decline in total cholesterol levels by 32.73%, 58.73% and 47.26%, 3) the positive control decreased total cholesterol levels by 39.46% and 4) total cholesterol levels on negative is unstable (there are up and some are down). The result of this research concluse that: guava leaf extract at dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg kgBB have an effect in lowering total cholesterol of rat had been induced prophylthiouracil. Keywords: guava leaves, total cholesterol. Abstrak: Penelitian ini bertujuan untuk mengetahui efek dari ekstrak etanol daun jambu biji terhadap kadar kolesterol total tikus wistar yang diinduksi propiltiourasil. Penelitian ini merupakan penelitian eksperimental. Sampel yang digunakan berjumlah 15 tikus wistar yang dibagi menjadi 5 kelompok (1 kelompok terdiri dari 3 tikus wistar), yaitu: 1) kontrol positif, 2) ekstrak dosis 200 mg/kgBB, 3) ekstrak dosis 400 mg/kgBB, 4) ekstrak dosis 800 mg/kgBB dan 5) kontrol negatif. Kelompok 1 sampai 4 diberikan propiltiourasil selama 14 hari, kemudian diberikan perlakuan sesuai kelompoknya. Hasil penelitian yang diperoleh: 1) rata-rata kadar kolesterol total meningkat setelah pemberian propiltiourasil yaitu dari 156,34 mg/dL menjadi 247,84 mg/dL, 2) pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB menunjukkan terjadi penurunan kadar kolesterol total sebesar 32,73%, 58,73% dan 47,26%, 3) pada kontrol positif terjadi penurunan kadar kolesterol total sebesar 39,46% dan 4) kadar kolesterol total pada kelompok negatif tidak stabil (ada yang naik dan ada yang turun). Hasil penelitian ini menyimpulkan bahwa ekstrak daun jambu biji pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB mempunyai efek dalam menurunkan kadar kolesterol total tikus wistar yang telah diinduksi propiltiourasil. Kata kunci: daun jambu biji, kadar kolesterol total.

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Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v18i01.12880 ◽

2021 ◽

Vol 18 (1) ◽

pp. 23-37

Author(s):

Madyawati Latief ◽

Indra Lasmana Tarigan ◽

Putri Maya Sari ◽

Fiolita Etsa Aurora

Keyword(s):

Uric Acid ◽

Point Of Care ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Test Method ◽

Point Of Care Test ◽

Randomized Design ◽

Wistar Strain ◽

Percent Decline

Hyperuricemia is a disease caused by an increase in uric acid levels in the blood. Currently, various treatments are developed by utilizing natural ingredients as an anti-hyperuricemia. Sungkai (Peronema canescens Jack) is one of the plants that contain a natural compound that can use as an anti-hyperuricemia treatment is the leaves of sungkai (Peronema canescens Jack.). The aim of this study to determine the anti-hyperuricemia activity of the ethanol extract of Sungkai leaves. The test animals used in this study were male white mice Wistar strain. This study used a completely randomized design (CRD) with 5 groups consisting of negative control (Na CMC 0.5%) positive control (allopurinol 10 mg / kg BW), treatment 1 (extract 125 mg / kg BW), treatment 2 (extract 250 mg / kg BW) and treatment 3 (extract 500 mg / kg BW). The parameters seen were uric acid levels measured using the POCT (Point of Care Test) method and analyzed using One Way Anova statistical analysis and Duncan's continued test. Our results showed that the treatment group with a dose of 125-500 mg / Kg BW reduce uric acid levels in mice. The best dose was a dose of 500 mg / Kg BW in reducing uric acid levels in mice with percent decline of 38.66%.

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The Side Effect of the Melastoma malabathricum L Ethanol Extract on the Gonad Maturation of Female Orange Mud Crab (Scylla olivacea)

HAYATI Journal of Biosciences ◽

10.4308/hjb.25.4.188 ◽

2018 ◽

Vol 25 (4) ◽

pp. 188

Author(s):

Nuril Farizah ◽

Muhammad Zairin JR ◽

Latifah Kosim Darusman ◽

Arief Boediono ◽

Muhammad Agus Suprayudi

Keyword(s):

Medicinal Plants ◽

Group Treatment ◽

Ethanol Extract ◽

Ovary Development ◽

Gonad Maturation ◽

Average Weight ◽

Mud Crab ◽

Control Groups ◽

Melastoma Malabathricum ◽

And Control

The medicinal plants in aquaculture are currently being studied quite extensively by researchers. Medicinal plants can act as an inducer or aphrodisiac and an antifertility agent in gonad maturation. The aimed of the experiment was to known side effects of the herb M. malabathricum L. on maturation process in female mud crab (S. olivacea). This study used 40 crabs with an average weight of 200-250g. The experiment consists of two treatment groups. The controls group (A) that received 100 μl of aquadest and group treatment (B) received 100 μl of 2 mg/g of ethanol extract M. malabathricum L. were studied. For 5, 10, 15, and 20 days of experiment, crabs were anesthetized and sacrified. The ovary was collect for GSI and histology. The end of experiment days (20 day), the hemolymph was collect to used ELISA assay. The result showed, significant differences in treatment group and control groups (p<0.05) in terms the macroscopic morphology of ovary, the mean GSI and the diameter of oocytes. Histological showed the influence of extract M. malabathricum L. on inhibited the ovary development compared with the control groups. Based on this results, it is concluded that ethanol extract of M. malabathricum L. leaves at a dose of 2 mg/g act as an antifertility agent for ovary maturation of S. olivacea.

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UJI EFEKTIVITAS SALEP EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L.) TERHADAP PENYEMBUHAN LUKA SAYAT PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.9.2020.29283 ◽

2020 ◽

Vol 9 (2) ◽

pp. 284

Author(s):

Repatri A Bawotong ◽

Edwin De Queljoe ◽

Deby A Mpila

Keyword(s):

Wound Healing ◽

Jatropha Curcas ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Steroid Glycoside ◽

Treatment Groups ◽

Jatropha Curcas L ◽

White Rats ◽

Wistar Strain

ABSTRACTJatropha leaves (Jatropha curcas L.) contains alkaloid, saponin, tannin, terpenoid, steroid, glycoside, phenol compound and flavonoid through ethanol extract. Until today the community still used the plant as a wound healing medicine. The aim of this study is to examine the effectiveness of jatropha curcas L. leaf extract ointment for healing in male white rats wistar strain. Jatropha leaves extract ointment was tested on 5 which consists of 3 rats in each treatment groups, that is base ointment (negative control), ointment betadine (positive control), ointment extract of Jatropha leaves 10%, 20% and 40%. Observations has made until the 14th day. And data were tested using ANOVA (Analysis of Variants) and continued with LSD (Least Significant Different) test. Statistical test results showed a difference in the duration of wound healing in male white rats wistar strain between the ointment extract of jatropha leaf with ointment betadine and vaseline ointment. Keywords: Ointment, Jatropha extract (Jatropha curcas L.), Galur wistar, Wound.ABSTRAK Daun jarak pagar (Jatropha curcas L.) mengandung zat-zat alkaloid, saponin, tannin, terpenoid, steroid, glikosida, senyawa fenol dan flavonoid melalui ekstrak etanol. Hingga saat ini masyarakat masih menggunakan tanaman tersebut sebagai obat penyembuh luka. Penelitian ini bertujuan untuk menguji efektivitas salep ekstrak etanol daun jarak pagar terhadap penyembuhan luka sayat pada tikus putih jantan galur wistar. Salep ekstrak daun jarak pagar diujikan pada hewan uji tikus sebanyak 5 yang terdiri dari 3 ekor tikus disetiap kelompok perlakuan, yaitu basis salep (kontrol negatif), salep betadine (kontrol positif), salep ekstrak etanol daun jarak pagar 10%, 20% dan 40%. Pengamatan dilakukan sampai hari ke 14. Data diuji dengan menggunakan ANOVA (Analisis Of Variant) dan dilanjutkan dengan uji LSD (Least Significant Different). Hasil uji statistik menunjukan adanya perbedaan terhadap lamanya penyembuhan luka pada tikus putih jantan galur wistar antara sediaan salep ekstrak daun jarak pagar dengan salep salep betadine dan basis salep. Kata Kunci: Salep, Daun jarak pagar (Jatropha curcas L.), Galur wistar, Luka sayat.

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Difference in Level of Malondialdehyde, Total Cholesterol, and Triglyceride After Administration of Passion Fruit Seed’s Ethanol Extract in Wistar Rats

10.20944/preprints201907.0179.v1 ◽

2019 ◽

Author(s):

Dina Keumala Sari ◽

Marianne Marianne ◽

Sri Lestari ◽

Lidya Imelda Laksmi

Keyword(s):

Total Cholesterol ◽

Wistar Rats ◽

Ethanol Extract ◽

Negative Control ◽

Passion Fruit ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

Preclinical Trial ◽

Significant Difference

High oxidative stress in cells due to inflammation process or excessive cell proliferation would produce oxidants or free radicals with biomarkers, one of which is malondialdehyde (MDA). Passion fruit seed’s contain high antioxidant and are expected to decrease the level of cholesterol and MDA. The objective is to identify the effect of passion fruit seed’s ethanol extract in Wistar rats that have been fed with atherogenic feed. The method was preclinical trial (post-test control group design) in rats, by administering passion fruit seed’s ethanol extract for 14 days. This study used 26 male rats aged two months, divided into 5 groups. The result showed significant difference in MDA level which was found in group that was given passion fruit seed extract 10mg/kg BW with positive control group that was given standard feed. Passion fruit seed’s extract showed significant difference in level of triglyceride, which was found in negative control group that was given atherogenic feed with group that was given passion fruit seed’s extract 5mg/kg BW (mean±standard deviation: 1.09±0.30 mg/dL vs 0.77±0.25mg/dL; p=0.048). This study showed that passion fruit seed’s ethanol extract had significant lowering effect in level of MDA, total cholesterol, and triglyceride for 14 days.

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AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

CHEMISTRY PROGRESS ◽

10.35799/cp.13.2.2020.31894 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Devy Angreani M ◽

Meiske Sangi ◽

Feti Fatimah

Keyword(s):

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Inflammatory Activity ◽

Control Group ◽

Treatment Groups ◽

White Rats ◽

Wistar Strain ◽

Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

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UJI AKTIVITAS ANTIDIABETES KOMBINASI GLIBENKLAMID DAN EKSTRAK DAUN SALAM (Syzygium polyanthumWight.) TERHADAP MENCIT (Mus musculus) YANG DIINDUKSI ALOKSAN

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2015.v1.i1.4831 ◽

2015 ◽

Vol 1 (1) ◽

pp. 42-47

Author(s):

Steffi Liem ◽

Yuliet Yuliet ◽

Akhmad Khumaidi

Keyword(s):

Blood Glucose ◽

Herbal Medicine ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Study Data ◽

Pharmacological Therapy ◽

Glucose Levels ◽

Blood Glucose Levels ◽

The Difference

Pharmacological therapy of Diabetes Mellitus (DM) is to use antidiabetic oral. Herbal medicine are also often used as an alternative treatment by patients. Even in some cases found patients combine drugs and herbal medicine to speed up the healing of DM. This study aims to know the effects of a combination of glibenclamide and bay leaf ethanol extract (BLEE) to decrease blood glucose levels and to determine its optimal combination dose as antidiabetic. The method was inducted alloxan at dose of 120 mg/kg BW intraperitoneally. The animal test used were mice which divided into 8 groups of tests, the negative control (Na CMC 0,5 %), positive control (glibenclamide 0,65 mg/kg BW), combination 1(glibenclamide+BLEE 250 mg/kg BW), combination 2 (glibenclamide+BLEE 500 mg/kg BW), combination 3 (glibenclamide+BLEE 750 mg/kg BW), BLEE 1 (250 mg/kg BW), BLEE 2 (500 mg/kg BW), and BLEE 3 (750 mg/kg BW). After 14 days study, data were analyzed by ANOVA showed the difference in decrease of blood glucose levels combination 2 (230±23,69) mg/dL and combination 3 (233,75±9,93) mg/dL were significantly different to the negative control (4±6,82) mg/dL, positive control (150,75±11,34) mg/dL, combination 1 (170±10,51) mg/dL, BLEE 1 (134,5±4,61) mg/dL, BLEE 2 (151,25±6,72) mg/dL, and BLEE 3 (158,75±17,64) mg/dL. While combination 2 and combination 3 were not significantly different. Combination of glibenclamide and bay leaf ethanol extract decreased blood glucose levels greater than a single dosage glibenclamide or bay leaf extract. Optimal doses decreased blood glucose levels was combination 2.

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Uji Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Notika (Arcboldiodendron calosericeum Kobuski) Terhadap Kadar Kolesterol Total Tikus (Rattus norvegicus) Jantan Galur Wistar

Journal Syifa Sciences and Clinical Research ◽

10.37311/jsscr.v2i1.2704 ◽

2019 ◽

Vol 2 (1) ◽

pp. 1-10

Author(s):

Nuralifah Nuralifah ◽

Wahyuni Wahyuni ◽

Parawansah Parawansah ◽

Ulan Dwi Shintia

Keyword(s):

Total Cholesterol ◽

Cholesterol Level ◽

Blood Lipids ◽

Total Cholesterol Level ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Antioxidant Compounds ◽

Cholesterol Levels ◽

Fat Consumption

Hyperlipidemia is an increase of lipid due to excessive fat consumption, that the intake and alteration of lipid is not balanced. Notika are plants that contain secondary metabolites in the form of flavonoids, tannins, saponins and triterpenoids which are antioxidant compounds that are thought to have the effect of reducing blood lipids. This study aims to determine the effect of ethanol extract of leaves of Notika on decreasing total cholesterol levels in rats induced by egg and propylthiouracil (PTU). This research was experimental in 6 groups, namely 4 groups given ethanol extract of leaves of Notika at a dose of 50 mg/kgBB, 100 mg/kgBB, 200 mg/kgBB, and 300 mg/kgBB, one group was given Na CMC 0.5% as a negative control, and one group was given simvastatin 10 mg as a positive control. The results showed that the ethanol extract of the leaves of Notika significantly reduced the total cholesterol level of rats with a significant value 0.05 and 300 mg/kgBB is an effective dose to reduce total cholesterol level.

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The effect of ethanol extract from Portulaca oleracea on inhibiting total cholesterol on animal subjects

Pharmacy Education ◽

10.46542/pe.2021.212.2226 ◽

2021 ◽

pp. 22-26

Author(s):

Afifah Bambang Sutjiatmo ◽

Fahmy Ahsanul Haq ◽

Sulaeman Al Jati ◽

Suci Nar Vikasari

Keyword(s):

Body Weight ◽

Total Cholesterol ◽

Ethanol Extract ◽

Portulaca Oleracea ◽

Control Group ◽

High Cholesterol Diet ◽

High Cholesterol ◽

Cholesterol Levels ◽

Test Parameters ◽

Effect Test

Introduction: Hypercholesterolemia occurs when cholesterol levels in the blood increases. Traditionally, krokot (purslane, portulaca oleracea) is used to treat cardiovascular disease. Aim: This research evaluated the effect of purslane extract to inhibit increasing of cholesterol levels. Methods: The ethanol extract dosage of purslane was 27.5, 55, and 110 mg/kg body weight (bw) and simvastatin 1.8 mg/kg bw were used as comparisons. The anti-hyper cholesterol effect test was done by feeding a high cholesterol diet and drinks containing 0.01% propylthiouracil. The test parameters were body weight and total cholesterol levels on days 0, 7, 14, and 21. Results: The results showed that the extract was able to prevent the increase in body weight compared to the control group (p<0.05) and that it could inhibit the increase of total cholesterol levels at day 14 and 21 compared to control group (p<0.05) and equivalent to simvastatin (p>0.05).

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Anti-inflammatory activities of ethnomedicinal plants from Dayak Abai in North Kalimantan, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d180433 ◽

2017 ◽

Vol 18 (4) ◽

pp. 1556-1561 ◽

Cited By ~ 2

Author(s):

SWANDARI PARAMITA ◽

KHEMASILI KOSALA ◽

DZULKIFLI DZULKIFLI ◽

DEBY INDAH SAPUTRI ◽

ENGGAR WIJAYANTI

Keyword(s):

Medicinal Plants ◽

Healing Process ◽

Area Under The Curve ◽

Ethanol Extract ◽

Health Problems ◽

Negative Control ◽

Antiinflammatory Drugs ◽

Ethnomedicinal Plants ◽

Rat Paw Edema ◽

Anti Inflammatory

Paramita S, Kosala K, Dzulkifli D, Saputri DI, Wijayanti E. 2017. Anti-inflammatory activities of ethnomedicinal plants from Dayak Abai in North Kalimantan, Indonesia. Biodiversitas 18: 1556-1561. Inflammation is a normal process in the human body as a response to injury from the healing process. Meanwhile, chronic inflammation will cause new health problems to patients. Antiinflammatory drugs generally used for those conditions, have several side effects to patients. The objective of this research was to find alternative anti-inflammatory drugs, especially from natural sources. Three medicinal plants recorded from Dayak Abai in North Kalimantan, Indonesia for health problems caused by the inflammation process i.e. Amomum xanthophlebium Baker, Clerodendrum buchananii (Roxb.) Walp., and Donax canniformis (G.Forst.) K.Schum. were used as material in this research. The experimental method using carrageenan-induced rat paw edema was used followed by the resulting measurement using plethysmometer. The results showed that significant differences of AUC (area under the curve) with p = 0.001 (p < 0.05) were achieved between negative control, positive control, and treatment group with plant medicinal extracts. AUC of leaves ethanol extract of C. buchananii showed the strongest antiinflammatory activities. It could be concluded that the medicinal plants recorded from ethnomedicinal data from Dayak Abai in North Kalimantan, have anti-inflammatory activities, with C. buchananii as the most potential ones which could be further developed as a new source of the anti-inflammatory drug.

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