Cellulolytic Enzyme Production by Three Fungi Grown in a Ground Corn Cob Medium1
Trichoderma reesei NRRL 11236, Trichoderma viride ATCC 32630, Trichoderma viride ATCC 32098 and Myrothecium verrucaria ATCC 9095 were evaluated for the production of toxic substances with the fertile egg tests. Strains NRRL 11236, ATCC 32630, and ATCC 9095 produced no detectable toxic substance. However, when an oil extract was made of fermented corn cobs, strain ATCC 32098 produced a significant kill of 23.1% of the embryos. Cellulases (C1,Cx) and beta glucosidase production were produced using different ammonium salts as additional nitrogen sources to what the corn cobs contained. Temperatures (23° and 30°C) had no effect on beta glucosidase except for T. viride ATCC 32630 which produced significantly smaller quantities at 30°C than at 23°C.