Evaluation of Selected Nutrients and Contaminants in Distillers Grains from Ethanol Production in Texas

ABSTRACTThis article contains the results of the evaluation of distillers grain (DG) coproducts from different ethanol plants around the United States and supplemented in animal diets in Texas, based on samples analyzed from 2008 to 2014. The samples were assessed for concentration, occurrence, and prevalence of selected nutrients and contaminants. Protein and sulfur contents of DG were largely different between maize and sorghum coproducts, as well as wet distillers grain with solubles (WDGS) and dried distillers grain with solubles (DDGS), indicating a significant effect of grain feedstock and dry grind process stream on DG composition and quality. Salmonella was isolated in 4 DDGS samples of a total of 157 DG samples, a percentage (2.5%) that is lower than the percentage of Salmonella-positive samples found in other feed samples analyzed during the same period. A small amount of virginiamycin residue was found in 24 maize DDGS, 1 maize WDGS, and 2 sorghum DDGS samples of 242 samples in total. One sorghum DDGS sample of 168 DG samples was contaminated with animal protein prohibited for use in ruminant feed and was channeled to poultry feed. The concentrations of aflatoxin and fumonisin DG coproducts averaged 3.4 μg/kg and 0.7 mg/kg, respectively. Among contaminated maize DG samples, five DDGS samples for aflatoxin contained a higher concentration than the U.S. Food and Drug Administration (FDA) minimum action level of 20 μg/kg for use in animal feed, whereas no sample for fumonisin was found above the action level of 5 mg/kg. The study provides the most current results involving DG coproducts and associated hazards that will assist development of food safety plans required by the FDA in their September 2015 rule titled “Current Good Manufacturing Practice Hazard Analysis and Risk Based Preventive Controls for Food for Animals.”

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Processing Technologies and Cell Wall Degrading Enzymes To Improve Nutritional Value of Dried Distillers Grain with Solubles for Animal Feed: an in Vitro Digestion Study

Journal of Agricultural and Food Chemistry ◽

10.1021/jf4019855 ◽

2013 ◽

Vol 61 (37) ◽

pp. 8821-8828 ◽

Cited By ~ 20

Author(s):

Sonja de Vries ◽

Annemieke M. Pustjens ◽

Mirjam A. Kabel ◽

Sergio Salazar-Villanea ◽

Wouter H. Hendriks ◽

...

Keyword(s):

Cell Wall ◽

Nutritional Value ◽

Animal Feed ◽

In Vitro Digestion ◽

Cell Wall Degrading Enzymes ◽

Processing Technologies ◽

Degrading Enzymes ◽

Distillers Grain ◽

Dried Distillers Grain

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ALIH TEKNOLOGI PENGOLAHAN PANGAN LOKAL DI KENAGARIAN ANDALEH, LIMAPULUH KOTA

LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat ◽

10.25077/logista.5.1.81-86.2021 ◽

2021 ◽

Vol 5 (1) ◽

pp. 81

Author(s):

Prima Yaumil Fajri ◽

Nela Eska Putri ◽

Rilma Novita ◽

Gusmalini Gusmalini ◽

Yenni Muchrida

Keyword(s):

Food Processing ◽

Animal Feed ◽

New Products ◽

Good Manufacturing Practice ◽

Poultry Feed ◽

Local Foods ◽

Village Community ◽

Dent Corn ◽

Corn Processing ◽

Local Dent

Jagung banyak dibudidayakan di Kenagarian Andaleh di Kecamatan Luak, Kabupaten Limapuluh Kota. Jenis jagung yang dibudidayakan adalah jagung gigi kuda yang sering dijadikan sebagai pakan ternak terutama pakan unggas. Jagung gigi kuda memiliki biji berbentuk gigi, mengandung karbohidrat (pati) yang tinggi, dan bertekstur keras. Ciri khas jagung ini adalah memiliki biji yang melekuk di bagian tengah atau bagian atas biji. Kegiatan ini bertujuan untuk meningkatkan pengetahuan masayarakat Nagari Andaleh Kecamatan Luak, tentang pemanfaatan bahan pangan lokal, khususnya komoditi jagung ini melalui alih teknologi pengolahan menjadi nugget jagung. Kegiatan diawali dengan memberikan penyuluhan tentang praktik sanitasi, meliputi sanitasi pekerja, sanitasi peralatan, dan sanitasi pengolahan, serta cara pengolahan pangan yang baik. Nugget jagung dibuat dari campuran daging ayam dan jagung dengan perbandingan 1:0.2. Melalui kegiatan ini, masyarakat mampu menerapkan higieni dan sanitasi dalam pengolahan pangan, serta memiliki keahlian dalam pengolahan jagung menjadi produk pangan baru, yaitu nugget jagung, sehingga meningkatkan pemanfaatan bahan pangan lokal dan meningkatkan perekonomian masyarakat. Dengan alih teknologi pemanfaatan bahan pangan lokal jagung diharapkan tumbuh motivasi untuk mengembangkan usaha skala rumah tangga yang berpengaruh pada peningkatan perekonomian masyarakat di Kenagarian Andaleh. Kata kunci: Jagung Gigi Kuda, Nagari Andaleh, Nugget Jagung, Alih Teknologi, Pangan Lokal ABSTRACT Corn is widely cultivated in Andaleh Village, Luak Sub-district, Lima Puluh Kota Regency. The type of corn that cultivated in this village is dent corn (Zea mays var. indentata), which often used as animal feed, especially poultry feed. Dent corn has a tooth-shaped seed, contains high carbohydrates (starch), and has a hard texture. The characteristic of this corn form is that the seeds have an indentation in the middle or top of the seed. The purpose of this programs was to increased the knowledge Andaleh Village community, Luak District, about the utilization of local food, especially dent corn through the transition technology of corn processing to be corn nuggets. The activity began with provided counseling on sanitation practices, including personal sanitation, equipment sanitation, and processing sanitation, as well as good manufacturing practice. Corn nuggets were made from a mixture of chicken and corn in a ratio of 1: 0.2. Through this programs, the community will be able to apply hygiene and sanitation in food processing, and have expertise in processing corn into new products like nuggets, thereby can increase the utilization of local foodstuffs and improve the community's economy. Transition technology to use local dent corn was expected to grow the motivation of community to develop household-scale businesses that have an effect to increase the economy of the Andaleh Village community. Keywords: Dent Corn, Andaleh Village, Corn Nugget, Transition of Technology, Local Foods

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Occurrence of Aflatoxin B1 in Animal Feed Collected from the Northeastern Area of Morocco

World s Veterinary Journal ◽

10.54203/scil.2021.wvj74 ◽

2021 ◽

Vol 11 (4) ◽

pp. 587-593

Author(s):

Naoual Alahlah ◽

Mohammed El Maadoudi ◽

Nourredine Bouchriti ◽

Réda Triqui ◽

...

Keyword(s):

Aflatoxin B1 ◽

High Performance ◽

Animal Feed ◽

Mean Value ◽

Poultry Feed ◽

Fish Feed ◽

Animal Products ◽

Production Chain ◽

Significant Difference ◽

Feed Samples

The carry-over of contaminants from feed to animal products is an important issue in the animal production chain, therefore, the quality control of those animal products should include the control of the animal feed. The current study was carried out to assess the contamination levels of three types of animal feed (dairy animal feed, poultry feed, and fish feed) by Aflatoxin B1. A total of 68 animal feed samples were collected from the Northeastern Moroccan area (Tangier-Tétouan-AL Hoceima). The samples were extracted with a mixture of acetone/water. The sample extractions were filtered, diluted with phosphate-buffered saline, and applied to an immunoaffinity column. Aflatoxin B1 was eluted with methanol then analyzed by high-performance liquid chromatography with fluorescence detection, after post-column photochemical derivatization. The analytical results for the level of Aflatoxin B1 in the animal feed samples revealed an average presence of 44.12% for all analyzed samples. The concentrations were between 1.02 and 13.59 µg/Kg, with a mean value of 4.08 ± 3.11 µg/Kg. The results indicated that there was a significant difference across the three types of animal feeds regarding the concentrations of Aflatoxin B1.

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Oil Recovery from Fractionated Dried Distillers Grains with Solubles (DDGS) Using Enzymes

Processes ◽

10.3390/pr9091507 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1507

Author(s):

Md. Sanaul Huda ◽

Nurun Nahar ◽

Ewumbua Monono ◽

Sagar Regmi

Keyword(s):

Enzymatic Hydrolysis ◽

Oil Recovery ◽

Animal Feed ◽

Enzyme Hydrolysis ◽

Plant Design ◽

Distillers Grains With Solubles ◽

Design Changes ◽

Distillers Grain ◽

Heavy Fractions ◽

Dried Distillers Grain

Oil recovered from dried distillers grain with solubles (DDGS) can be a high-value product over animal feed to provide an additional profit to ethanol plants currently operating at slim profit margins. Fractionations of DDGS and enzymatic hydrolysis were considered in this study to improve the oil recovery from DDGS. A combination of sieving and then air aspiration was used to separate the original DDGS into three different fractions: light, medium, and heavy. The heavier fraction had up to 24% increased oil content compared to the original DDGS. Commercial enzymes, protease, cellulase, and hemicellulase were tested separately and in combinations at 55 °C for 3 h at 130 rpm to determine their effect on oil recovery from the original and fractionated DDGS. Oil recovery was significantly improved (around 20%) following enzyme hydrolysis of the sieved aspirated heavy fractions of DDGS compared to the original DDGS. More than 90% of oil recovery was achieved by using a combination of cellulase and protease enzymes. Increasing the temperature above 55 °C without any enzyme did not impact oil recovery using the heavy-fraction DDGS. Overall, fractionation and enzymatic hydrolysis showed promise to increase oil recovery from DDGS without any current ethanol plant design changes.

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The Politics of Precaution

10.23943/princeton/9780691124162.001.0001 ◽

2012 ◽

Cited By ~ 6

Author(s):

David Vogel

Keyword(s):

United States ◽

Animal Feed ◽

The United States ◽

Hazardous Substances ◽

Risk Regulation ◽

Scientific Certainty ◽

European Public ◽

Wide Range ◽

Regulatory Controls ◽

Milk Hormones

This book examines the politics of consumer and environmental risk regulation in the United States and Europe over the last five decades, explaining why America and Europe have often regulated a wide range of similar risks differently. It finds that between 1960 and 1990, American health, safety, and environmental regulations were more stringent, risk averse, comprehensive, and innovative than those adopted in Europe. But since around 1990 global regulatory leadership has shifted to Europe. What explains this striking reversal? This book takes an in-depth, comparative look at European and American policies toward a range of consumer and environmental risks, including vehicle air pollution, ozone depletion, climate change, beef and milk hormones, genetically modified agriculture, antibiotics in animal feed, pesticides, cosmetic safety, and hazardous substances in electronic products. The book traces how concerns over such risks—and pressure on political leaders to do something about them—have risen among the European public but declined among Americans. The book explores how policymakers in Europe have grown supportive of more stringent regulations while those in the United States have become sharply polarized along partisan lines. And as European policymakers have grown more willing to regulate risks on precautionary grounds, increasingly skeptical American policymakers have called for higher levels of scientific certainty before imposing additional regulatory controls on business.

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Physicochemical characterisation of kafirins extracted from sorghum grain and dried distillers grain with solubles related to their biomaterial functionality

Scientific Reports ◽

10.1038/s41598-021-94718-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Umar Shah ◽

Deepak Dwivedi ◽

Mark Hackett ◽

Hani Al-Salami ◽

Ranjeet P. Utikar ◽

...

Keyword(s):

Secondary Structure ◽

Physicochemical Properties ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Chemical Properties ◽

X Ray ◽

Distillers Grain ◽

Sorghum Grain ◽

Dried Distillers Grain ◽

Polypeptide Profile

AbstractKafirin, the hydrophobic prolamin storage protein in sorghum grain is enriched when the grain is used for bioethanol production to give dried distillers grain with solubles (DGGS) as a by-product. There is great interest in DDGS kafirin as a new source for biomaterials. There is however a lack of fundamental understanding of how the physicochemical properties of DDGS kafirin having been exposed to the high temperature conditions during ethanol production, compare to kafirin made directly from the grain. An understanding of these properties is required to catalyse the utilisation of DDGS kafirin for biomaterial applications. The aim of this study was to extract kafirin directly from sorghum grain and from DDGS derived from the same grain and, then perform a comparative investigation of the physicochemical properties of these kafirins in terms of: polypeptide profile by sodium-dodecyl sulphate polyacrylamide gel electrophoresis; secondary structure by Fourier transform infra-red spectroscopy and x-ray diffraction, self-assembly behaviour by small-angle x-ray scattering, surface morphology by scanning electron microscopy and surface chemical properties by energy dispersive x-ray spectroscopy. DDGS kafirin was found to have very similar polypeptide profile as grain kafirin but contained altered secondary structure with increased levels of β-sheets. The structure morphology showed surface fractals and surface elemental composition suggesting enhanced reactivity with possibility to endow interfacial wettability. These properties of DDGS kafirin may provide it with unique functionality and thus open up opportunities for it to be used as a novel food grade biomaterial.

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71 Effects of timing of supplementation during late gestation in Bos indicus-influenced beef cows on post-weaning growth, immunity, and carcass characteristics of steer progeny

Journal of Animal Science ◽

10.1093/jas/skz397.135 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 59-59

Author(s):

Elizabeth Palmer ◽

Marcelo Vedovatto ◽

Juliana Ranches ◽

Vinicius Gouvea ◽

Rhaiza Oliveira ◽

...

Keyword(s):

Bos Indicus ◽

Carcass Characteristics ◽

Beef Cows ◽

Late Gestation ◽

Experimental Unit ◽

Carcass Quality ◽

Diarrhea Virus ◽

Randomized Design ◽

Distillers Grain ◽

Dried Distillers Grain

Abstract This study evaluated the growth, immunity, and carcass characteristics of beef steers born to cows offered dried distillers grains (DDG) at different timing during late gestation. Approximately 84 d before calving (d 0), 84 mature Brangus cows were stratified by BW and BCS (422 ± 42 kg; 5.0 ± 0.14) and allocated into 1 of 6 bahiagrass pastures (14 cows/pasture). Treatments were randomly assigned to pastures: 1) no prepartum supplementation (CON); 2) 1 kg/d DDG from d 0 to 84 (LATE84); or 3) 2 kg/d from d 0 to 42 (LATE42). Calves were weaned at 261 ± 23 d of age. At weaning, 38 steers were vaccinated against bovine viral diarrhea virus (BVDV-1) and parainfluenza-3 (PI-3) and transported to a feedlot facility. Steers were penned according to cow prepartum pasture distribution and fed a similar diet until slaughter (d 587). Data were analyzed as complete randomized design using MIXED procedure of SAS, pasture as the experimental unit, treatment as fixed effect, and pasture(treatment) and steer(pasture) as random effects. Steer feedlot BW, ADG, DMI, and G:F did not differ (P ≥ 0.12) among treatments. Plasma haptoglobin concentrations were greater (P ≤ 0.03) for LATE42 and LATE84 vs. CON steers 2 d after feedlot entry. Plasma cortisol and seroconversion for BVDV-1 and PI-3 did not differ (P ≥ 0.15) among treatments. The percentage of carcasses grading Choice did not differ (P = 0.13), but carcasses grading low Choice were greater (P = 0.03) for CON vs. LATE42, with LATE84 being intermediate (91, 40, and 58%, respectively). Thus, prepartum dried distillers grain supplementation to B. indicus-influenced cows did not enhance feedlot growth but had subtle positive impacts on carcass quality and innate immunity in steer progeny. In addition, timing of dried distillers grain supplementation (last 84 d vs. first 42 d of late gestation) did not impact steer post-weaning feedlot performance and carcass quality.

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22 Effects of Housing Cow-calf Pairs on Drylots vs Pasture on Calf Performance and Behavior Through Weaning

Journal of Animal Science ◽

10.1093/jas/skab054.042 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 24-24

Author(s):

Megan Myerscough ◽

Wes Chapple ◽

William T Meteer ◽

Daniel W Shike

Keyword(s):

Corn Silage ◽

Time Interaction ◽

Creep Feed ◽

Calf Performance ◽

Ad Libitum ◽

Cow Calf ◽

Distillers Grain ◽

Dried Distillers Grain ◽

And Behavior ◽

Fence Line

Abstract The objectives were to analyze the effects of housing cow-calf pairs in drylots or pasture on calf performance and behavior through weaning. Simmental × Angus (2 yr; 108/yr; 81 ± 15.3 d postpartum) spring-calving cows were stratified by age, BW, BCS, and calf sex and allotted to six groups/yr. Groups were randomly allotted to 1 of 2 treatments: drylot (DL) or pasture (PAST). Calves in the drylot had ad libitum access to a diet consisting of corn silage, dried distillers grain, corn stalks, dry rolled corn, and soybean hulls. Calves on PAST received creep feed three weeks prior to weaning. Calf BW was measured on d 0, 55, and 110. Calves were fence-line weaned on d 110. Behavior was observed for two days after weaning. Data were analyzed using the MIXED procedure of SAS. Drylot calves had greater (P < 0.01) BW at d 55 and 110. There was treatment by time interactions (P < 0.01) for lying and eating on d 111. More PAST calves were lying at h 2 and 3 and eating at h 11 and 12. More DL calves were lying at h 9, 10, 11, and 12. More DL calves were eating at h 2 and tended to at h 3. There was also a treatment by time interaction (P < 0.01) for vocalizations on d 111. More PAST calves vocalized at h 1, however, DL calves vocalized more at h 4 and tended to at h 5. There was treatment by time interactions (P < 0.01) for eating and walking on d 112. More DL calves were eating at h 2, 3, 4, 7, and 9. More PAST calves were walking at h 1 and 2. There was also a treatment by time interaction (P < 0.01) for vocalizations on d 112. More PAST calves vocalized at h 1 and 10. In conclusion, housing cow-calf pairs in drylots improved preweaning performance and altered postweaning behavior.

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43 Cow Performance and Lameness Are Affected by Housing in Drylots vs Pasture During the Summer

Journal of Animal Science ◽

10.1093/jas/skab054.251 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 147-148

Author(s):

Lucas Hofer ◽

Megan Myerscough ◽

Wes Chapple ◽

Travis T Meteer ◽

Keela Trennepohl ◽

...

Keyword(s):

Milk Production ◽

Binary Data ◽

Body Condition Score ◽

Hair Coat ◽

Milk Protein Content ◽

Condition Score ◽

Milk Urea ◽

Cow Performance ◽

Distillers Grain ◽

Dried Distillers Grain

Abstract The objective was to compare the performance of cows housed in drylots or on pasture. Spring-calving, Simmental × Angus cow-calf pairs (n = 108; 77 ± 18 days postpartum) were stratified by age, body weight (BW), body condition score (BCS), calving date, and calf sex. Cows were allotted into six groups which were randomly assigned to drylot or pasture. Drylot cows were limit-fed a ration consisting of corn silage, dried distillers grain, corn stalks, cracked corn, and a corn-based supplement to meet protein and energy requirements. Cows on pasture were rotationally grazed with access to free-choice mineral. Cows were artificially inseminated on day 0. Cow BW, BCS, hair coat scores, locomotion scores, and lameness treatments were evaluated throughout the 110-day experiment. Milk production and composition were evaluated on day 56. Data were analyzed using the MIXED and GLIMMIX (binary data) procedures of SAS. Artificial insemination and overall pregnancy rates did not differ (P ≥ 0.79) between groups. Drylot cows were 22 and 51 kg heavier (P ≤ 0.02) than pasture cows on days 83 and 110, respectively. Drylot cow BCS was greater (P = 0.03) on day 110. Hair coat scores were more desirable (P = 0.03) in drylot cows than pasture cows on day 110. Drylot cows had greater (P = 0.04) milk production than pasture cows. Pasture cows had greater (P ≤ 0.03) milk protein content and milk urea nitrogen. Although locomotion scores did not differ (P ≥ 0.45) on days 0 and 34, they were less desirable (P = 0.02) for the drylot cows on day 110. A greater (P = 0.02) percentage of drylot cows (33%) were treated for lameness than pasture cows (7%). Housing cows in drylots increased BW, BCS, and milk production, but resulted in poorer locomotion scores and increased lameness treatments.

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