ANTIBACTERIAL STUDY OF SHEETAMSHU RASA AND ITS MODIFIED METH-ODS
Knowledge of microorganisms was well stablished in Ayurveda in terms of krimi since Vedic period and have told that the organisms were invisible, scattered and present everywhere in which some are harmful or harmless to human beings. In Samhita clear cut description of invisible krimi is found and also said that they are causes various diseases like Jawar, Kushtha considered as contagious disease by Accharya Sushruta. Number of Ayurvedic formulation possess krimighna (antimicrobial) properties. Therefore, need of study to evaluate antimicrobial property of Ayurvedic formulations due to resistance of antibiotic which is challenge for modern medical science. Sheetamshu Rasa (SR) is one of the unique herbomineral formu-lation explained in text Basavarajiyam under Sannipataja Jwara Rogadhikara and also it has many indica-tions. Four samples of SR were prepared according to textual reference and slight modification in leviga-tion media & ratio of ingredients. Antimicrobial study was done in different concentrations in DMSO, against three bacteria Klebsiella pneumonia, Pseudomonas aeruginosa & Staphylococcus aureus produc-ing fever as a symptom by Agar well diffusion method and Ciprofloxacin (5 μg/ml) as antibacterial stand-ards. Result shows that SR samples had antibacterial activity moderate to high. SR1 and SR2 showed the best result in higher concentration than the other two samples (ZI= 11mm and AI= 0.92) on Klebsiela pneumonia. Trikatu and levigation media have no special significance in antibacterial activity.