Uji Aktivitas Antikolesterol Ekstrak Etanol Daun Jambu Air(Syzygium aqueum (Burm.f.)Alston) SecaraIn Vitro

2021 ◽  
Vol 1 ◽  
pp. 839-849
Author(s):  
Miranda Noviani ◽  
S Slamet ◽  
W Wirasti ◽  
Urmatul Waznah
Keyword(s):  
Ethanol Extract ◽  
Activity Analysis ◽  
Test Solution ◽  
Percent Reduction ◽  
Flavonoid Compounds ◽  
Cholesterol Levels ◽  
Body Tissues ◽  
Guava Leaves ◽  
Guava Leaf

AbstractCholesterol is a natural substance that has physical properties similar to fat but has the formula steroids. Cholesterol belongs to the non-hydrolyzed lipid group and is the main sterol in body tissues. When cholesterol levels increase, it can cause blockages in blood vessels. Several studies have explained that one of the compounds that can reduce cholesterol is flavonoid compounds, one of the plants containing flavonoid compounds is guava leaf (Syzygium aqueum (Burm.f.) Alston). The purpose of this study was to determine the activity and percent reduction in cholesterol levels and the EC50 value of the ethanol extract of guava leaves (Syzygium aqueum (Burm.f.) Alston) in vitro using UV-Vis spectrophotometry with a wavelength of 665 nm. Anticholesterol activity analysis was carried out using Lieberman-Burchard reagent with a test solution series of 150; 300; 450; 600; and 750 ppm. The results showed that the greater the concentration, the lower the absorbance produced and the higher the percent reduction in cholesterol levels. At a concentration of 750 ppm cholesterol decreased by 58.74%. And the obtained EC50 value of 462 ppm, which means at that concentration the ethanol extract of guava leaves (Syzygium aqueum (Burm.f.) Alston) can reduce 50% of the initial cholesterol.Keywords:Cholesterol, Guava leaf, In Vitro, Spectrophotometry UV-Vis, Syzygium aqueum (Burm.f.)Alston. AbstrakKolesterol merupakan suatu zat alami yang memiliki sifat fisik hampir sama dengan lemak tetapi memiliki rumus steroida. Kolesterol adalah sterol utama yang ada pada jaringan tubuh dan termasuk dalam golongan lipid yang tidak terhidrolisis. Apabila kadar kolesterol mengalami kenaikan, dapat menyebabkan penyumbatan pada pembuluh darah. Dari beberapa penelitian dijelaskan bahwa salah satu senyawa yang dapat menurunkan kolesterol adalah flavonoid, salah satu tanaman yang mengandung senyawa flavonoid adalah daun jambu air (Syzygium aqueum (Burm.f.)Alston). Tujuan dari penelitian ini adalah untuk mengetahui aktivitas dan persen penurunan kadar kolesterol serta nilai EC50 dari ekstrak etanol daun jambu air (Syzygium aqueum (Burm.f.)Alston) secara in vitro menggunakan spektrofotometri UV-Vis dengan panjang gelombang 665 nm. Analisis aktivitas antikolesterol dilakukan dengan menggunakan pereaksi Lieberman-Burchard dengan seri larutan sampeli 150; 300; 450; 600; dan 750 ppm. Hasil penelitian menunjukkan semakin besar konsentrasi, absorbansi yang dihasilkan adalah semakin rendah dan persen penurunan kadar kolesterolnya semakin tinggi. Pada konsentrasi 750 ppm terjadi penurunan kolesterol sebesar 58,74%. Dan diperoleh nilai EC50 sebesar 462 ppm yang artinya pada konsentrasi tersebut ekstrak etanol daun jambu air (Syzygium aqueum (Burm.f.)Alston) dapat menurunkan 50% dari kolesterol awal.Kata kunci: Daun jambu air;Kolesterol;In Vitro;Spektrofotometri UV-Vis;Syzygium aqueum (Burm.f.)Alston

scholarly journals UJI EFEK EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava L) TERHADAP KADAR KOLESTEROL TOTAL TIKUS WISTAR (Rattus norvegicus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Irianto Girik Allo ◽  
Pemsi Mona Wowor ◽  
Henoch Awaloei
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
Guava Leaves ◽  
Guava Leaf

Abstract: This research aims to determine the effect of ethanol extract of guava leaves on levels of total cholesterol of rat that is induced propylthiouracil. This was an experimental research. The sample used was 15 rats were divided into 5 groups (1 group is consist of 3 rats), namely: 1) positive control, 2) extract dose of 200 mg/kgBB, 3) extract dose of 400 mg/kgBB, 4) extract dose of 800 mg/kgBB and 5) negative control. Group 1 to 4 are given propylthiouracil given for 14 days, then given treatment appropiate with the group. The result show that: 1) the average of total cholesterol levels increased after administration propylthiouracil from 156.34 mg/dL to 247.84 mg/dL, 2) at a dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB showed a decline in total cholesterol levels by 32.73%, 58.73% and 47.26%, 3) the positive control decreased total cholesterol levels by 39.46% and 4) total cholesterol levels on negative is unstable (there are up and some are down). The result of this research concluse that: guava leaf extract at dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg kgBB have an effect in lowering total cholesterol of rat had been induced prophylthiouracil. Keywords: guava leaves, total cholesterol.     Abstrak: Penelitian ini bertujuan untuk mengetahui efek dari ekstrak etanol daun jambu biji terhadap kadar kolesterol total tikus wistar yang diinduksi propiltiourasil. Penelitian ini merupakan penelitian eksperimental. Sampel yang digunakan berjumlah 15 tikus wistar yang dibagi menjadi 5 kelompok (1 kelompok terdiri dari 3 tikus wistar), yaitu: 1) kontrol positif, 2) ekstrak dosis 200 mg/kgBB, 3) ekstrak dosis 400 mg/kgBB, 4) ekstrak dosis 800 mg/kgBB dan 5) kontrol negatif. Kelompok 1 sampai 4 diberikan propiltiourasil selama 14 hari, kemudian diberikan perlakuan sesuai kelompoknya. Hasil penelitian yang diperoleh: 1) rata-rata kadar kolesterol total meningkat setelah pemberian propiltiourasil yaitu dari 156,34 mg/dL menjadi 247,84 mg/dL, 2) pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB menunjukkan terjadi penurunan kadar kolesterol total sebesar 32,73%, 58,73% dan 47,26%, 3) pada kontrol positif terjadi penurunan kadar kolesterol total sebesar 39,46% dan 4) kadar kolesterol total pada kelompok negatif tidak stabil (ada yang naik dan ada yang turun). Hasil penelitian ini menyimpulkan bahwa ekstrak daun jambu biji pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB mempunyai efek dalam menurunkan kadar kolesterol total tikus wistar yang telah diinduksi propiltiourasil. Kata kunci: daun jambu biji, kadar kolesterol total.

scholarly journals PENGARUH EKSTRAK ETANOL DAUN JAMBU AIR (SYZYGIUM AQUEUM) TERHADAP BAKTERI ISOLAT KLINIS

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Titi Hariyati ◽  
Dwi Soelistya Dyah Jekti ◽  
Yayuk Andayani
Keyword(s):  
Ethanol Extract ◽  
Clinical Isolates ◽  
E Coli ◽  
Randomized Design ◽  
Inhibition Concentration ◽  
The Family ◽  
Ethanol Extracts ◽  
Guava Leaves ◽  
Guava Leaf

Syzygium aqueum plant of the family Myrtaceae is native of Malaysia and Indonesia and is known as the water rose. The active compound is useful as an antibacterial. This study aimed to determine the effects of ethanol extracts of guava leaves (S. aqueum) as an antimicrobial against clinical isolates bacteria in vitro. The design used in experiment was a Completely Randomized Design (CRD). Data were analyzed using the Kruskal-Wallis test. Results of statistical analysis using SPSS 20 for windows indicated that the ethanol extract of guava leaves has a significant (P<0,05) effect in inhibiting the growth of clinical isolates bacteria. MIC (Minimum Inhibition Concentration) of ethanol extracts for each bacterium can not be determined because the guava leaf extract was very dark. The MBC (Minimum Bacterisidal Concentration) of ethanol extract of guava leaf  for both S. aureus and S. dysenteriae was 20%.  The MBC of the extract for E. coli, S. thypi, V. cholerae was 25%, where as for B. cereus was at concentration of 50%. It can be concluded that guava leaf has highly potential as a source of antimicrobial agent.Keywords:Antibacteria, Guava Leaf, Clinical Isolates Bacteria, Ethanol Extract

scholarly journals FORMULASI DAN UJI PELEPASAN KRIM EKSTRAK ETANOL DAUN JAMBU BIJI DENGAN POTENSI ANTIJERAWAT

Jurnal Kimia ◽  
2020 ◽  
pp. 119
Author(s):  
N. P. Y. A. Dewi ◽  
N. L. G. W. Pebriani ◽  
P. A. Duarsa ◽  
P. C. I. Warnaya ◽  
I. D. A. A. D. Candraningrat ◽  
...  
Keyword(s):  
Stearic Acid ◽  
Chemical Properties ◽  
Ethanol Extract ◽  
Physical And Chemical Properties ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Optimum Formula ◽  
Physical And Chemical ◽  
Active Substances ◽  
And Chemical Properties

Guava leaves contain quercetin which can inhibit the growth of Propionibacterium acne. Thus, guava leaves have the potential to be anti-acne. Based on the benefits of guava leaves, it is necessary to formulate the ethanol extract of guava leaves in cream preparation and its characterization as well as to know the rate of releasing active substances. Preparation and standardization of guava leaf simplicia was carried out. After that, maceration, standardization and phytochemical screening of guava leaf ethanol extract were done. Positive extracts containing flavonoids were formulated into cream preparation. The concentration of stearic acid as emulgator of 14% and 18% was optimized. The formula was tested for physical and chemical properties such as organoleptic, spreadability, adhesion, viscosity and pH. The optimum formula was characterized using Franz diffusion test to determine the release of the active substance. The results showed that concentration of stearic acid as emulgator influenced the physical and chemical properties of the cream. Based on the results of this study it could be concluded that the optimum formula was stearic acid concentration of 14% with the release active substances for 3 hours of 2,5882 mg. Keywords: Guava leaves, acne, cream, stearic acid, diffusion

scholarly journals Aktivitas In Vitro Penurun Gula Darah dari Ekstrak Buah Parijoto (Medinilla speciosa B.)

2020 ◽  
Vol 31 (1) ◽  
pp. 31
Author(s):  
Rissa Vifta ◽  
NFN Wilantika ◽  
Yustisia Dian Advistasari
Keyword(s):  
Block Design ◽  
Enzymatic Reaction ◽  
Ethanol Extract ◽  
Complementary Therapy ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Flavonoid Compounds ◽  
Treatment And Prevention ◽  
Ethanol Extracts

Diabetes mellitus is one of the highest causes of death in the world, with symptoms of increased blood glucose levels (hyperglycemia). One of the efforts made as a treatment and prevention of DM is through complementary therapy using natural ingredients. Parijoto fruit (<em>Medinilla speciosa</em>) contains flavonoid compounds potential to reduce glucose levels. The study aimed to analyze the effectiveness of ethanol extracts and its fractions of parijoto fruit (EEBP) to decrease glucose levels in vitro. The study was conducted at the Ngudi Waluyo University Laboratory in April to August 2018. Identification and screening of flavonoid compounds from ethanol extracts and its fractions of parijoto fruit were performed qualitatively and by thin-layer chromatography (TLC). <em>In vitro</em> testing for  antidiabetic activity of EEBP was performed with non-enzymatic reaction by Nelson Somogyi method (ethanol extract and its fractions). Experiment  was arranged in a randomized block design, with 12 treatments and three replications. The concentrations of parijoto fruit tested were 10 ppm, 20 ppm, 30 ppm, 40 ppm, 50 ppm, and 60 ppm. The results of qualitative identification and TLC showed that the ethanol extract and its fraction of EEBP contained flavonoid compounds. At a concentration of 30 ppm, EEBP was able to reduce glucose levels by 42.43 %, while the ethanol fraction decreased glucose levels by 83.38 %. The results showed parijoto fruit ethanol extract and its fractions were sources of antidiabetic. Hence its pharmacologically effect was necessary to be further studied.

scholarly journals In Vitro Determination of SunProtective Factor (SPF) of Dadap Serep (Erythrina Subumbrans (Haks.) Merr.) Leaf Extract Using Spectrophotometric Method

2019 ◽  
Vol 1 (1) ◽  
pp. 64-67
Author(s):  
Indri Maharini
Keyword(s):  
Bioactive Compounds ◽  
Spectrophotometric Method ◽  
Leaf Extract ◽  
Protective Factor ◽  
Ethanol Extract ◽  
Flavonoid Compounds

Dadap serep (Erythrina Subumbrans Haks Merr) contains bioactive compounds of flavonoids, saponins, isoflavones, alkaloids, and lectins. Flavonoid compounds have a sunscreen effect. This studyaimed to determinethe value of Sun Protective Factor (SPF) using spectrophotometry method with variations in the extract concentrations, such as500 ppm,750 ppm, and 1000 ppm.The results showed that the SPF values of each concentration were11.47 ±0.583335, 17.05 ± 0.898838,21.93 ± 0.791557, respectively. In conclusion, the ethanol extract of dadap serep hastheability to become sunscreen in the maximal and ultra category.

scholarly journals Preliminary Phitochemical Analysis Of Guava Leaves (Psidium guajava L.) Extract As Antidiarrheal In Calves

2019 ◽  
Vol 3 (2) ◽  
pp. 21
Author(s):  
I Wayan Sudira ◽  
I Made Merdana ◽  
Suci Nur Qurani
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
The West ◽  
Active Compounds ◽  
Central Java ◽  
Guava Leaves ◽  
Guava Leaf

Guava plants (Psidium guajava L) are fruit plants originating from South America and can thrive in the territory of Indonesia. Now the biggest center for guava cultivation is spread in DKI Jakarta, West Java, Central Java, East Java, Yogyakarta, Bali, West Nusa Tenggara, Sumatra and Kalimantan. In addition to taking the fruit, guava leaves are also used as a traditional medicine for the treatment of diarrhea in human and animals. This study aims to identify active compounds in guava leaves. That is a secondary  metabolites with pharmacological effects as anti diarrhea such as flavonoid, alkaloid, tannins and essential oils. The leaves sample used in this study were taken from farmers in the West Denpasar area, Bali. Extraction of guava leaf leaves using 96% ethanol, and phytochemical analysis was carried out to detect the presence of active compounds. The data obtained is presented and analyzed descriptively qualitatively. The results showed that the ethanol extract of guava leaves contained active compound inclaude steroid/triterpenoid, phenolics, alkaloids, flavonoids, saponins and tannins. It can be concluded that guava leaf extract contains are potential antidiarrheal

scholarly journals In Vitro Antidiabetic Activities of Extract and Isolated Flavonoid Compounds from Artocarpus altilis (Parkinson) Fosberg

2014 ◽  
Vol 14 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Puspa Dewi Narrij Lotulung ◽  
Tjandrawati Mozef ◽  
Chandra Risdian ◽  
Akhmad Darmawan
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Inhibition Mechanism ◽  
Artocarpus Altilis ◽  
Flavonoid Compounds ◽  
Mechanism Of Inhibition ◽  
Ethyl Acetate Extracts

The antidiabetic activity test through a mechanism of inhibition of α-glucosidase enzyme was studied against ethanol, n-hexane, ethyl acetate and n-butanol fractions of ethanol extract of Artocarpus altilis (Parkinson) Fosberg (Moraceae) leaves and four flavonoid compounds isolated from ethyl acetate extracts of A. altilis. Ethyl acetate fraction has strongest antidiabetic activity compared to ethanol, n-hexane, and n-butanol fractions with IC50values5.98,6.79, 440.18and14.42μg/mL, respectively. Four flavonoid compounds (1-(2,4-dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-1-benzopyran-5-yl]-1-propanone (AC-31), 2-geranyl-2',3,4,4'-tetrahydroxy dihydrochalcone (AC-51), 8-geranyl-4',5,7-trihydroxyflavone (AC-33) andcyclocommunol (AA-3), have been isolated from ethylacetate fraction. AC-31 was the strongest antidiabetic compound compared to AC-51, AC-33 and cyclocommunolwithIC50values are 15.73, 24.41,49.49,and72.20μg/mL. Kineticstudies of AC-31 using Lineweaver-Burk method showed that inhibition mechanism of enzymeα-glucosidase was anon-competitivetype.

scholarly journals Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering

2018 ◽  
Vol 21 (2) ◽  
pp. 54-58 ◽  
Author(s):  
Devina Ingrid Anggraini ◽  
Lily Fathrah Nabillah
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
The Body ◽  
Flavonoid Content ◽  
Cholesterol Lowering ◽  
Cholesterol Levels ◽  
Method Of Extraction ◽  
Lowering Activity ◽  
Solvent Analysis

Cholesterol is a natural substance with physical characteristic similar to fat but has a steroidal group. The body requires cholesterol in normal amount; however, it will harm the body in excess amount. High cholesterol levels in the blood are dangerous because of the precipitation of cholesterol and other fatty substances resulting in atherosclerosis. Suji leaf (Dracaena angustifolia Roxb.) used as a natural dye has a high flavonoid content that is inferred to have cholesterol-lowering activity. This study aims to test the in vitro activity of suji leaf (Dracaena angustifolia Roxb.) extract in decreasing cholesterol level with various concentrations and to find the effective concentration (EC50). The method of extraction used was remaceration method with 70% ethanol solvent. Analysis of cholesterol-lowering activity was done by Lieberman-Burchard method by making variation of ethanol extract 400 ppm, 500 ppm, 600 ppm, 700 ppm, and 800 ppm. The results showed the percentage of cholesterol-lowering activity by 33.62%, 36.15%, 46.61%, 56.39% and 64.05% respectively. Value of EC50 activity of suji leaf extract is 632.50 ppm.

scholarly journals ANTI-CHOLOSETEROL ACTIVITIES OF WHITE (RAPHANUS RAPHANISTRUM) AND RED (RAPHANUS SATIVUS) RADISH ROOTS

2021 ◽  
pp. 24-29
Author(s):  
NOVI YANTIH ◽  
WINDA DESTIANA ◽  
DIAH KARTIKA PRATAMI
Keyword(s):  
Statistical Analysis ◽  
Raphanus Sativus ◽  
Ethanol Extract ◽  
Raphanus Raphanistrum ◽  
Cholesterol Levels ◽  
Radish Root ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Red Radish

Objective: The purpose of this research is to explore the in vitro activities of white (Raphanus raphanistrum) and red (Raphanus sativus) radish root ethanol extract in decreasing cholesterol levels. Methods: Ultrasonification method was used in obtaining 96% ethanol extract of white and red radish root. The cholesterol levels were analyzed by visible spectrophotometry, which was validated using Lieberman-Buchard reagents. The decreased cholesterol levels were converted into IC50 values. Results: The results showed that the IC50 of 96% white and red radish root ethanol extracts were 743.7 and 634.7 µg/ml, respectively. The results of statistical analysis using the T-test obtained a significant value greater than the probability value (P) of 0.05. Conclusion: Therefore, it was concluded that the activities of 96% ethanol extract of white and red radish roots did not have a significant ability to reduce the in vitro cholesterol levels.

scholarly journals Effect of Psidium guajava L on Biofi lm Forming Multidrug Resistant Extended Spectrum Beta Lactamase (ESBL) Producing Pseudomonas aeruginosa

2019 ◽  
Vol 6 ◽  
pp. 19-25
Author(s):  
Bhagawati Khadka ◽  
Moni Mahato ◽  
Reshma Tuladhar ◽  
Anjana Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Disk Diffusion Method ◽  
Extended Spectrum Beta Lactamase ◽  
Guava Leaves

Objectives: In order to investigate the relative efficacy of Psidium guajava L (guava) leave, in vitro antibacterial effect of ethanolic extract and leaf tea of guava against pathogenic Pseudomonas aeruginosa was carried out. Methods: This study was carried out at Microbiology laboratory of KIST medical college and teaching hospital, Lalitpur. Pseudomonas aeruginosa was isolated from different clinical samples. Antibiotic susceptibility of the isolates was performed by Kirby-Bauer disk diffusion method. Biofilm formation was detected by microtitre culture plate method and ESBL production by combine disk methods. Antimicrobial activity of guava leaf tea and ethanol extract of leaf were determined by agar well diffusion method. Results: Ethanol extract of fresh guava leaves exhibited higher antibacterial activity than dry and fresh leaves tea, but significantly less than the standard antibiotics. In this study, 7% of the total bacterial isolates were P. aeruginosa with 65.30% of these isolates being MDR. Similary, significant number of these MDR strains ie. 83.67% of these P. aeruginosa isolates produced biofilm. While as 6.12% of the isolated were ESBL producer. Conclusion: The leaves extract of guava have shown effective result against P. aeruginosa and could serve as good source of antibacterial agents. Guava leaves extract can be an economic alternative to antibiotics. However, active compound of this extract need to be purified and pharmacologically tested before its application.

Sign in / Sign up

Export Citation Format

Share Document