SPECTROPHOTOMETRIC AND HPTLC STUDIES ON SELECTED INDIAN SPICE EXTRACTS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (08) ◽  
pp. 30-34
Author(s):  
P. Soni ◽  
◽  
V. Soni ◽  
P. Sharma
Keyword(s):  
Yield Loss ◽  
Total Phenolics ◽  
Major Peak ◽  
Drug Formulations ◽  
Extract Yield ◽  
Total Alkaloids ◽  
Phytochemical Compounds ◽  
Rf Values ◽  
Phytochemical Constituents ◽  
Cinnamon Zeylanicum

The present work was carried out with a view to analyze the major phytochemical compounds of Indian spices, dalchini (Cinnamon zeylanicum) bark and coriander (Coriander sativum) fruit. Hydro-alcoholic extracts were prepared from selected spices and analyzed for extract yield, loss on drying (LOD) and pH. phytochemical compounds such as total alkaloids, phenols, flavonoids and tannins were quantified by spectrometric methods. Hydro-alcoholic extracts from selected spices revealed the pH to be between 5.36-5.62, loss on drying 12.54–15.41% and extract yield 8.62–29.19%w/w. Among the investigated samples, higher levels of alkaloids (2.63%) and flavanoids (3.42%) were found in C. sativum while C. zeylanicum exhibited high content of total phenolics (3.35%) and tannins (0.82%). The hydro-alcoholic extracts were subjected to HPTLC analysis and the results suggested the presence of two different major phytochemical compounds in C. zeylanicum, whereas Coriander sativum extract displayed three major peak. C. sativum extracts showed relatively similar spots with Rf values, which indicates the presence of four similar types of flavonoids in each extract. Due to the presence of various phytochemical constituents and favorable extract yield, LOD and pH, the presently investigated spice extracts can be used in drug formulations.

scholarly journals Review Based Upon Ayurvedic and Traditional Uses of Cinnamomum tamala (Tejpatta)

2021 ◽  
Vol 68 (2) ◽  
Author(s):  
Shifali Thakur ◽  
Gitika Chaudhary
Keyword(s):  
Chemical Constituents ◽  
Bioactive Constituents ◽  
Pharmacological Activities ◽  
The Past ◽  
Cinnamomum Tamala ◽  
Medicinal Use ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
Herbal Plant ◽  
Flavoring Agent

Cinnamomum tamala is an evergreen plant native to Sri Lanka and India. This herbal plant is commonly called Indian cassia, Tejpatta and Indian bay leaf. There are many bioactive constituents isolated from plant Cinnamomum tamala. Leaves of the plant possess aromatic fragrance and are also utilized as a flavoring agent. It is used in food curry, pickles and other spices. Besides food application, the leaves have also been used for curing a number of ailments. All parts of the plant possess many major bioactive chemical constituents like cinnamaldehyde, trans-cinnamaldehyde, 3,4,5,7- tetrahydroxyflavone, 3,3,4,5,6- pentahydroflavone (non-glycoside compounds), kaempferol, eugenol, etc. These phytochemical compounds have many pharmacological activities such as anticancer, antidiabetic, antimicrobial, hepatoprotective, antidiarrheal and immunomodulatory. In the past time, it is also considered for medicinal use. Their medicinal use was also mentioned in the literature of ayurveda, yunani and other traditional systems of medicine. Due to aromatic fragrance, it is also utilized in the perfume industry. The major aim of this review is to give a brief knowledge about the plant Cinnamomum tamala based on their phytochemical constituents, ayurvedic view, folk view and pharmacological application.

scholarly journals GC–MS analysis of phytoconstituents from Amomum nilgiricum and molecular docking interactions of bioactive serverogenin acetate with target proteins

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Narasimhamurthy Konappa ◽  
Arakere C. Udayashankar ◽  
Soumya Krishnamurthy ◽  
Chamanalli Kyathegowda Pradeep ◽  
Srinivas Chowdappa ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Ethyl Acetate ◽  
Plant Species ◽  
In Silico ◽  
Leaf Extract ◽  
Target Proteins ◽  
Methanol Extracts ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
In Silico Molecular Docking

Abstract Amomum nilgiricum is one of the plant species reported from Western Ghats of India, belonging to the family Zingiberaceae, with ethno-botanical values, and is well-known for their ethno medicinal applications. In the present investigation, ethyl acetate and methanol extracts of A. nilgiricum were analyzed by Fourier transform infrared spectrometer (FTIR) and gas chromatography-mass spectrometry (GC–MS) to identify the important functional groups and phytochemical constituents. The FTIR spectra revealed the occurrence of functional characteristic peaks of aromatic amines, carboxylic acids, ketones, phenols and alkyl halides group from leaf and rhizome extracts. The GC–MS analysis of ethyl acetate and methanol extracts from leaves, and methanol extract from rhizomes of A. nilgiricum detected the presence of 25 phytochemical compounds. Further, the leaf and rhizome extracts of A. nilgiricum showed remarkable antibacterial and antifungal activities at 100 mg/mL. The results of DPPH and ferric reducing antioxidant power assay recorded maximum antioxidant activity in A. nilgiricum methanolic leaf extract. While, ethyl acetate leaf extract exhibited maximum α-amylase inhibition activity, followed by methanolic leaf extract exhibiting aldose reductase inhibition. Subsequently, these 25 identified compounds were analyzed for their bioactivity through in silico molecular docking studies. Results revealed that among the phytochemical compounds identified, serverogenin acetate might have maximum antibacterial, antifungal, antiviral, antioxidant and antidiabetic properties followed by 2,4-dimethyl-1,3-dioxane and (1,3-13C2)propanedioic acid. To our best knowledge, this is the first description on the phytochemical constituents of the leaves and rhizomes of A. nilgiricum, which show pharmacological significance, as there has been no literature available yet on GC–MS and phytochemical studies of this plant species. The in silico molecular docking of serverogenin acetate was also performed to confirm its broad spectrum activities based on the binding interactions with the antibacterial, antifungal, antiviral, antioxidant and antidiabetic target proteins. The results of the present study will create a way for the invention of herbal medicines for several ailments by using A. nilgiricum plants, which may lead to the development of novel drugs.

scholarly journals Assessment of Phytochemical Constituents and Antimicrobial Activity of Lantana Camara L.

2017 ◽  
Vol 14 (3) ◽  
pp. 977-984
Author(s):  
Ajay Kumar ◽  
Shikha Singh ◽  
Pragati Saini
Keyword(s):  
Spectroscopic Analysis ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Lantana Camara ◽  
Proteus Vulgaris ◽  
Zone Of Inhibition ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
Microsporum Fulvum ◽  
Bacteria And Fungi

ABSTRACT: In present study the phytochemical constituents such as total phenol, total flavonoid contents and antibacterial activity against four gram negative and two gram positive isolates Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogens, Proteus vulgaris, Lactobacillus, S. aureus, Bacillus subtilis and antifungal activity against four fungal strains Penicillium, Aspergillus niger, T. mentagrophytes, Microsporum fulvum of petroleum ether, diethyl ether, chloroform and acetone extract of leaves and flowers of Lantana camara L were evaluated. Maximum zone of inhibition was recorded in the presence of free flavanoid fraction of the plant extract against Trichophyton mentagophytes and Microsporum fulvum which was the most susceptible fungus for all the extracts tested. The extract also compared favourably with streptomycin which serves as a positive control. Minimum inhibitory concentration (MIC) was recorded for all bacteria and fungi in which highest MIC was of B. subtilis and M. fulvum. The UV-Vis and FTIR spectroscopic analysis also revealed the presence of different active groups and bonds. L. camara contains phytochemical compounds with antibacterial & antifungal activities. Moreover, the chloroform & acetone leaf & flower extracts of L. camara are active against pathogenic microorganisms.

scholarly journals Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 90-94
Author(s):  
P Sasikala ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
M Padma ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fine Powder ◽  
Ethanol Extract ◽  
Taraxacum Officinale ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Biological Compounds ◽  
Phytochemical Compounds ◽  
Medicinal Properties ◽  
Phytochemical Constituents

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial  and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various  medicinal  properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.

scholarly journals GC-MS Analysis of Bioactive Compounds and Phytochemical Evaluation of the Ethanolic Extract of Gomphrena globosa L. Flowers

2020 ◽  
Vol 10 (2) ◽  
pp. 53-58
Author(s):  
Azmat Unnisa Esmat ◽  
Sirisha Mittapally ◽  
Safiya Begum
Keyword(s):  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Pharmacological Activities ◽  
Ms Analysis ◽  
Gomphrena Globosa ◽  
Whole Plant ◽  
Docosanoic Acid ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
Anti Inflammatory

Gomphrena globosa (L.) has high medicinal values. All parts are been used as folk medicines. The extract of whole plant / flowers has shown different phytochemical constituents with various clinical properties. These phytochemicals recognised are responsible for various activities such as anti-inflammatory, anticancer, antibacterial, analgesic and cytotoxic. The ethanolic extract of Gomphrena globosa L. flowers of biochemical test indicates the presence of sterols, triterpenoid, tannins, phenols and flavonoids, and on GC-MS analysis it has shown 11 phytochemical compounds with different pharmacological activities. The major bioactive compounds are Docosanoic Acid, Docosyl Ester (25.404%) and Hexatriacontane (24.324%), has proven anti-inflammatory activity. Keywords: Gomphrena globose L., phytochemical constituents, GC-MS and anti-inflammatory.

scholarly journals Proximate Composition, Mineral Content and Secondary Metabolites of Three Medicinal Wild Fagonia Species

Food Biology ◽  
2020 ◽  
pp. 1-6
Author(s):  
Mustafa M. El-Zayat ◽  
Yasser Ahmed El-Amier ◽  
El-Sayed F. El-Halawany ◽  
Iman A. Abo Aisha
Keyword(s):  
Secondary Metabolites ◽  
Proximate Composition ◽  
Nutritional Value ◽  
Mineral Content ◽  
Total Phenolics ◽  
Methanolic Extract ◽  
Phytochemical Composition ◽  
Total Alkaloids ◽  
Aerial Parts ◽  
Micro Elements

Proximate composition of the aerial parts of three Fagonia species (Fagonia arabica L., F. mollis Delile and F. cretica L.) collected from different habitats were analyzed. Macro- and micro-elements as well as some secondary metabolites were estimated. The obtained results revealed that F. creticus contains appreciable levels of nutritive components considering that its nutritional value (351.06 kcal/100g dry wt.) was remarkably higher than that of F. arabica and F. mollis (327.99 and 293.07 kcal/100g dry wt., respectively). The concentration of Na was relatively the highest among the other estimated macroelements in the studied species followed by K, Ca and Mg, respectively while Fe was the highest microelement followed by Cu, Mn and Zn, respectively. The phytochemical composition revealed that methanolic extract of F. creticus was the richest in total alkaloids and flavonoids, while F. arabica found to be the richest in total phenolics and tannins.

scholarly journals PENGARUH JENIS PELARUT TERHADAP EKSTRAKSI DARI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)

KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 271-279
Author(s):  
Amalia Noviyanty ◽  
Chitra Anggriani Salingkat ◽  
Syamsiar Syamsiar
Keyword(s):  
Total Phenolics ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Fruit Peel ◽  
Extract Yield ◽  
Randomized Design ◽  
Acetone Water ◽  
Dragon Fruit ◽  
Ic50 Value ◽  
Ic50 Values

This study aims to determine the effect of the type of solvent on extract yield, total phenolics and IC50 values of the skin of red dragon fruit and also obtain the best type of solvent that provides extract rendement, total phenolics and the highest or best IC50 value for extracting red dragon fruit peels. There are 3 types of solvents used (95% ethanol, ethyl acetate and acetone: water (7: 3) with a sample-solvent ratio (5:1 v/b).  The data obtained were analyzed using a Completely Randomized Design that was applied to observations of extract rendemen, phenolics total and IC50 values, if the treatment had a very significant or significant effect followed by continued BNJ test at 1% or 5% level. The results showed that the solvent ratio very significantly affected the extract rendemen, phenolics total and IC50 value of red dragon fruit peel extract.  Ethanol 95% solvent is the best solvent for extracting dragon fruit peels because it has the highest yield (26.15%), total phenolic (64.75 ppm) and antioxidant activity (IC50 value) (120.53 ppm) highest or best. Keywords : IC50, the red dragon fruit skin, solvent ratio, extract yield, phenolics total

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND HPTLC FINGER PRINTING ANALYSIS OF TRADITIONAL MEDICINAL PLANT PUERARIA TUBEROSA (ROXB. EX WILLD.)DC

2018 ◽  
Vol 5 (1) ◽  
pp. 56-59
Author(s):  
Zereena Viji ◽  
Paulsamy S
Keyword(s):  
Medicinal Plant ◽  
Acetone Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Pueraria Tuberosa ◽  
Medicinal Value ◽  
Percentage Area ◽  
Finger Printing ◽  
Phytochemical Compounds ◽  
Rf Values

The medicinal value of a plant lies in the phytoconstituents present in it. These phytochemical compounds form the base of modern drugs. The aim of the present study is to identify the phytochemical constituents present in the traditional medicinal plant Pueraria tuberosa and to develop HPTLC fingerprint profile of acetone extract. Preliminary phytochemical screening was done to identify the phytoconstituents and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5applicator, TLC scanner 3, Reprostar 3 and WINCATS-1.4.3 software were used. The present study revealed the presence ofcarbohydrates, proteins alkaloids, flavonoids, saponins, phenols and tannins in various extracts. The HPTLC fingerprint analysis of acetone extract of Pueraria tuberosa showed 10 peaks at 254nm. The components with Rf values 0.05, 0.21 and 0.72 were predominant with the percentage area of 34.52, 16.16 and 10.10respectively. The preliminary phytochemical analysis revealed the presence of various phytochemicals, which were confirmed by the HPTLC fingerprint profile

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF METHANOLIC LEAF EXTRACT OF DRYPETES SEPIARIA (WIGHT & ARN.) PAX. & HOFFIM. FROM SILAMBUR SACRED GROVE, TAMILNADU

2017 ◽  
Vol 4 (2) ◽  
pp. 157-161
Author(s):  
Saravanakumar K
Keyword(s):  
Extraction Method ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Sacred Grove ◽  
Phytochemical Screening ◽  
Plant Materials ◽  
Ms Analysis ◽  
Successive Extraction ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents

Drypetes sepiaria (Wight & Arn.) Pax. & Hoffim a medium sized tree member of Euphorbiaceae was investigated to determine the phytochemical constituents present in various extracts of the leaves through GC-MS analysis. Powdered leaf plant materials were subjected to successive extraction with organic solventssuch as methanol by Soxhlet extraction method. In the present study, GC-MS analysis revealed that a total of 23 different compounds identified by using methanol extract and all the identified compounds were medicinally valuable for the treatment of various human ailments. In addition, all the phytochemical compounds were needed for further investigations on toxicological aspects for the development of new lead of therapeutic interest.

Preliminary Phytochemical Screening, Isolation, Characterization, Structural Elucidation and Antibacterial Activities of Leaves Extracts Rhus Vulgaris (Kimmo)

2021 ◽  
Author(s):  
Aderaw Anteneh ◽  
Getachew G/Mariam W/Hana ◽  
Desta Shumuye Meshesha
Keyword(s):  
Antibacterial Activity ◽  
1H Nmr ◽  
13C Nmr ◽  
Antibacterial Activities ◽  
Solvent Mixture ◽  
Solvent Ratio ◽  
Herbal Plants ◽  
Rf Values ◽  
Phytochemical Constituents ◽  
Isolated Compound

Abstract Background: Rhus vulgaris commonly known as sumac, a plant that is known to possess different therapeutic values including antioxidant and antibacterial activities. Medicines from plants contributed largely to human health. The aim of this study was to screen the phytochemical constituents, isolate, elucidate the structure and antibacterial activity of methanol extract from the leaves of Rhus vulgaris.Methods: The methanolic extract of Rhus vulgaris was subjected to column chromatography and eluted with solvent mixture of methanol: chloroform (1:8) ratio. The eluted fractions were run in the TLC mobile phase with the different solvent ratio. Based on the TLC profile the fractions with similar Rf values were pooled together. The structure of the isolated compound was characterized based on the spectral data (IR, 1H NMR, 13C NMR, and DEPT) and extracts from Rhus vulgaris has been shown to have antibacterial activity were tested against four strains bacteria Streptococcus aureus(gram-positive) and Escherichia coli, Salmonella typhimurium, and K. pneumoniae (gram-negative) using Agar well diffusion method.Result: The results showed that the methanol extracts were active against all the tested bacteria. The structure of this compound 1-p-tolyl pentadeca-7,9-dien-1-ol was characterized by means of 1H NMR, 13C NMR, and IR spectral data.Conclusion: Therefore, it is concluded that the use of herbal plants and their recipes are the major source of drugs in a traditional medicinal system to cure different diseases.

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