scholarly journals Feasibility of bioethanol production from rice bran

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 2951-2966
Author(s):  
Francieli Begnini Siepmann ◽  
◽  
Daneysa Lahis Kalschne ◽  
Caroline Zabotti ◽  
Eder Lisandro de Moraes Flores ◽  
...  
Keyword(s):  
Rice Bran ◽  
Alcoholic Fermentation ◽  
Starch Content ◽  
Bioethanol Production ◽  
Inoculum Concentration ◽  
Positive Effects ◽  
Alternative Sources ◽  
Optimized Conditions ◽  
Sequential Strategy ◽  
Central Composite

Rice bran is a by-product of rice production with a high carbohydrate and starch content and the potential for bioethanol production by alcoholic fermentation. This article describes bioethanol production by Saccharomyces cerevisiae from hydrolyzed defatted rice bran (DRB) a rice by-product applying ultrasonic treatment and protease addition, as well as a sequential strategy of experimental design (SEED). In the first Central Composite Rotatable Design (CCRD), the temperature (25-30 °C) and inoculum concentration (0.5-50 g L-1) had positive effects on bioethanol production, while the effect of pH (4.0-6.0) was not significant. In the second CCRD, the temperature (28-35 °C) and inoculum concentration (10-70 g L-1) had negative and positive effects on bioethanol production (p < 0.05). Protease addition (15 µL g-1) increased the conversion of substrate into bioethanol by 76%. The optimized conditions for the production of 40.7 g L-1 bioethanol were a temperature of 31.5 °C and an inoculum concentration of 70 g L-1. Validation in a benchtop bioreactor produced 40.0 g L-1 of bioethanol from hydrolyzed DRB, and the SEED was characterized as a useful tool to improve bioethanol production from DRB. Furthermore, the DRB proved to be a by-product with great potential for bioethanol production, derived from alternative sources not commonly used in human food.

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia Sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-Wastes for Bioethanol Production

2021 ◽  
Author(s):  
R.V. Beladhadi ◽  
Kumar Shankar ◽  
S.K. Jayalakshmi ◽  
kuruba sreeram
Keyword(s):  
Rice Bran ◽  
High Titer ◽  
Value Added ◽  
Bioethanol Production ◽  
Waste Biomass ◽  
Production Methods ◽  
Corn Husk ◽  
Novel Bacterium ◽  
Optimized Conditions ◽  
First Time

Abstract Purpose: This study focuses to assess the potentiality of the locally isolated Burkholderia sp SMB1. It was aimed for the cocktail of lignocellulolytic enzyme production involved in the depolymerization of the agro-wastes to release sugars for bioethanol production. Methods: The optimization of enzyme cocktail (lignolytic, cellulolytic, hemicellulolytic) production by isolated bacterium utilizing inexpensive substrates like bran and straw of rice was done using response surface methodology. Further this cocktail secreted by the bacterium at optimized conditions was employed for saccharifying untreated agro-wastes for sugars production which were fermented to bioethanol by S. cerevisiae.Results: This strain produced high titer of cellulase (10.8 U/mL), xylanase (76 U/mL), mannanase (14.23 U/mL), pectinase (62.18 U/mL) and laccase (24.25 U/mL) using rice bran at 7 pH and 40 oC using 10% (w/v) of rice bran. The production of cocktail of enzymes was slightly increased by adding CaCO3 (2mM) in the growth medium. This cocktail was able to hydrolyze untreated agro-wastes to release highest reducing sugars 28.5 g/L using untreated corn husk after 24 h of saccharification process. These sugars of corn husk were fermented by S. cerevisiae to produce highest 9.04 gL-1 bioethanol. Conclusion: This paper represents the exploitation of the locally isolated strain for the production of lignocellulolytic enzymes using rice bran and straw for the first time promising the novelty of the study. This study helps in waste disposal process by valorizing the waste biomass to produce value added products like sugars, bioethanol and hence increases agricultural economy.

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

2016 ◽  
Vol 27 (1) ◽  
pp. 22-35 ◽  
Author(s):  
Elsa Cherian ◽  
M. Dharmendira Kumar ◽  
G. Baskar
Keyword(s):  
Design Methodology ◽  
Simultaneous Saccharification And Fermentation ◽  
Agricultural Waste ◽  
Cellulase Production ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Corn Cob ◽  
Content Type ◽  
Optimized Conditions ◽  
Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

Drum drying of unpeeled green banana pulp (Musa cavendishii) for bake stable fruit filling production.

2019 ◽  
Vol 36 (1) ◽  
Author(s):  
Mário José Andrade MENDES ◽  
Dayane Rosalyn IZIDORO ◽  
Agnes De Paula SCHEER
Keyword(s):  
Chemical Composition ◽  
Central Composite Design ◽  
Resistant Starch ◽  
Starch Content ◽  
Pasting Properties ◽  
Absorption Capacity ◽  
Production Chain ◽  
Banana Flour ◽  
Green Banana ◽  
Central Composite

There is a growing interest in food matrixes for the use of flour from unpeeled green banana in order to reduce waste in the production chain. Considering this, the present paper aims to studying the application of unpeeled green banana flour in the cold process performed to obtain bake stable fruit filling. The unpeeled green banana pulp (Musa Cavendishii) was dried using a single rotating drum, thus obtaining the flour. The dried flour was then analyzed for its chemical composition, amylose and resistant starch content, water absorption capacity and pasting properties. The drying reduced the amount of resistant starch and produced pregelatinized starch. The obtained flour showed physical and nutritional characteristics which enabled the development of the filling formula by using a central composite design combining levels -1 and +1, two axial points (± α), two central points, and chemical composition, water activity, Brix, and texture as response variables. The amount defined by central-composite design of unpeeled green banana flour, modified starches and other ingredients resulted in an elastic, viscous, bake stable fruit filling.

scholarly journals A study on alkali pretreatment conditions of sorghum stem for maximum sugar recovery using statistical approach

2014 ◽  
Vol 20 (2) ◽  
pp. 261-271 ◽  
Author(s):  
Maryam Nikzad ◽  
Kamyar Movagharnejad ◽  
Farid Talebnia ◽  
Ghasem Najafpour ◽  
Farahi Hosein
Keyword(s):  
Sorghum Bicolor ◽  
Alternative Energy ◽  
Solid Loading ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Model Predictions ◽  
Pretreatment Time ◽  
Pretreatment Conditions ◽  
Energy Production System ◽  
Central Composite

Bioethanol production from lignocellulosic biomass provides an alternative energy-production system. Sorghum bicolor stem is a cheap agro-waste for bioethanol production. In this study, response surface methodology (RSM) was used to optimize alkali pretreatment conditions for sorghum bicolor stem with respect to substrate concentration, NaOH concentration and pretreatment time based on a central composite rotary design. The main goal was to achieve the highest glucose and xylose yields after enzymatic hydrolysis. Under optimum conditions of pretreatment i.e. time 60.4 min, solid loading 4.2%, and NaOH concentration 1.7%, yields of 98.94% g glucose/g cellulose and 65.14% g xylose/g hemicelluloses were obtained. The results of a confirmation experiment under the optimal conditions agreed well with model predictions. Pretreatment of sorghum bicolor stem at the optimum condition increased the glucose and xylose yields by 7.14 and 3.02 fold, respectively. Alkali pretreatment showed to be a great choice for the pretreatment of sorghum bicolor stem.

scholarly journals Acid and Enzymatic Hydrolyses of Rice Bran for Bioethanol Production

ALCHEMY ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Dewi Yuliani ◽  
Khoirul Achmad Julianto ◽  
Akyunul Jannah
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrochloric Acid ◽  
Acid Hydrolysis ◽  
Sulphuric Acid ◽  
Rice Bran ◽  
Dilute Hydrochloric Acid ◽  
Cellulolytic Enzyme ◽  
Bioethanol Production ◽  
By Products ◽  
Sugar Source

<p class="BodyAbstract">Rice bran is one among many agricultural by-products containing ~50-60 wt.% of carbohydrate. The carbohydrate is a prominent sugar source for bioethanol production. The objective of this research was to study bioethanol production from rice bran by acid and enzymatic treatment. The variations of acid used were dilute hydrochloric acid and sulphuric acid, while variations of enzyme used were amylolytic and cellulolytic enzyme. Ethanol production of acid-hydrolyzed rice bran was 24.95±1.61% (v/v) by hydrochloric acid and 29.57±2.04% (v/v) by sulphuric acid. Ethanol produced by enzymatic hydrolysis was quite low i.e. 6.7±0.04%, and 8.86±0.29% (v/v) for amylolytic and cellulolytic hydrolysate, respectively.</p><p class="BodyAbstract"> </p><p>Keywords: Bioethanol, rice bran, acid hydrolysis, enzymatic hydrolysis</p>

Evaluation of Ultrasound Waves on S. cerevisiae Stimulation in the Bioethanol Production from Rice Bran

2020 ◽  
Vol 13 (1) ◽  
pp. 314-324 ◽  
Author(s):  
Mônica Stobienia ◽  
Daneysa Lahis Kalschne ◽  
Bianca Peron-Schlosser ◽  
Luciane Maria Colla ◽  
Ilton José Baraldi ◽  
...  
Keyword(s):  
Rice Bran ◽  
Bioethanol Production ◽  
Ultrasound Waves

scholarly journals Carrageenan-Based Films Incorporated with Jaboticaba Peel Extract: An Innovative Material for Active Food Packaging

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5563
Author(s):  
Luisa Bataglin Avila ◽  
Elis Regina Correa Barreto ◽  
Paloma Krolow de Souza ◽  
Bárbara De Zorzi Silva ◽  
Thamiris Renata Martiny ◽  
...  
Keyword(s):  
Food Packaging ◽  
Extraction Process ◽  
Assisted Extraction ◽  
Active Food Packaging ◽  
Light Barrier ◽  
Innovative Material ◽  
Bioactive Potential ◽  
Optimized Conditions ◽  
Central Composite ◽  
Peel Extract

This research investigated the bioactive potential of jaboticaba peel extract (JPE) and proposed an innovative material for food packaging based on carrageenan films incorporated with JPE. The extract was obtained through microwave assisted extraction (MAE) according to central composite rotational design and the optimized conditions showed a combined antimicrobial and antioxidant actions when the extraction process is accomplished at 80 °C and 1 min. The carrageenan film incorporated with JPE was manageable, homogeneous and the presence of JPE into film increased the thickness and improved the light barrier of the film. The results of solubility and mechanical properties did not show significant differences. The benefit of using MAE to improve the recovery of bioactive compounds was demonstrated and the carrageenan film with JPE showed a great strategy to add additives into food packaging.

scholarly journals Influence of Lactose Concentration in Bioethanol Production from Cheese Whey

2017 ◽  
Vol 39 (5) ◽  
pp. 533
Author(s):  
Cleidiane Samara Murari ◽  
Débora Cristina Moraes Niz da Silva ◽  
Bruna Lima da Silva ◽  
Vanildo Luiz Del Bianchi
Keyword(s):  
Alcoholic Fermentation ◽  
Cheese Whey ◽  
Ethanol Yield ◽  
Oxygen Demand ◽  
Bioethanol Production ◽  
Organic Substances ◽  
Promising Alternative ◽  
Renewable Source ◽  
Initial Concentration ◽  
Lactose Concentration

The present study aimed the utilization of the cheese whey as substrate for ethanol production by Kluyveromyces marxianus.  Was studied the effect of the initial concentration of cheese whey (M1 57,6 g L-1; M2 45,6 g L-1; M3 32,5 g L-1 e M4 18,8 g L-1) on the alcoholic fermentation. After sterilization, the medium were incubated at 30ºC for 48 hours, performing analysis of lactose, proteins, ethanol, cell growth and chemical oxygen demand. According to the results, the medium M1 (the highest concentration) showed a higher production and productivity of ethanol 16.9 g L-1 and 1.26 g L.h-1, respectively, and also obtained the highest production and productivity of cell of 5.8 g L-1 and 0.40 g L.h-1, respectively. However, in terms of ethanol yield, the most satisfactory result was obtained with the M3 medium with 82.30% in 12 hours of fermentation. The organic substances content has been reduced in relation to COD in the medium M4 in 82.28%, representing a promising alternative for valorization of cheese whey as an effective alternative to obtain a renewable source of biofuel.

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