QUORUM SENSING SCREENING OF SOME MEDICINAL PLANTS FROM MONGOLIA

2015 ◽  
Vol 13 (2) ◽  
pp. 63-65 ◽  
Author(s):  
B Zolboo ◽  
L Tsenguunmaa ◽  
O Undram ◽  
J Batkhuu
Keyword(s):  
Quorum Sensing ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Aerial Part ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacterial Communication ◽  
Standard Disc

The purpose of this study was to search for a novel quorum sensinginhibitor and analyseits inhibitory activity of medicinal plants of Mongolia. This study investigated the effects of some plant extracts on the bacterial communication system, expressed as quorum sensing activity. Quorum sensinghas directly propotional effect on the amount of certain compound such as pigments, produced by the bacteria. Alcohol extracts of 103 extracts of 66 medicinal plants were tested for anti-quorum sensingactivity by the Chromobacterium violaceum assay using the standard disc-diffusion method. The screening revealed the anti-quorum sensing activity of 18 extracts of 13 plants; particularly aerial part of Hedysarum alpinum L., Spongiocarpella gruboviiUlzii and Goniolimon speciosum (L.) Boiss.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 63-65

scholarly journals ANTIMICROBIAL ACTIVITY OF MIXTURE FROM DIFFERENT PLANT EXTRACTS IN GEL, CREAM AND BODY MILK FORMULATION, AGAINST DIFFERENT MICROORGANISMS

2017 ◽  
Vol 35 (2) ◽  
Author(s):  
Dzoko Kungulovski ◽  
Natalija Atanasova-Pancevska
Keyword(s):  
Antimicrobial Activity ◽  
Minimal Inhibitory Concentration ◽  
Plant Extracts ◽  
Broad Spectrum ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Inhibition Zones ◽  
Standard Disc

In this study the antimicrobial activity of a mixture of plant extracts originating from five different plants and suspended in three different forms of gel, cream and lotion was examined with the purpose of discovering new anti-microbial compounds. The antimicrobial activity was investigated through the standard disc diffusion method, as well as through a variation of the microdillution method. The formulations under examination (gel, cream and lotion) showed a broad spectrum of action against all the selected microorganisms, with inhibition zones of 9–46 mm. The minimal inhibitory concentration (MIC) for all the formulations against the microorganisms in the study was in the range of 0.000761μg ml-1 to 0.125μg ml-1. The results of this study have clearly demonstrated that the mixture of plant extracts originating from five different plants and suspended in the forms of gel, cream and lotion can definitely be used in the battle against the microorganisms under investigation.

scholarly journals IN-VITROSTUDIES ON ANTIFUNGALACTIVITY OF SOMEMISTLETOE SPECIES

2021 ◽  
Vol 9 (06) ◽  
pp. 420-427
Author(s):  
R. Neelima ◽  
◽  
B. Sujatha ◽  
N.V. Ramana ◽  
B.V. Rao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Phytophthora Infestans ◽  
Inhibitory Activity ◽  
Sclerotium Rolfsii ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts

In vitro evaluation of antifungal activity by disc diffusion method was carried out on leaf extracts of D.falcata, D. falcata var pubescens, V. monoicum and V. orientale and stem extracts of D. falcata, D. falcata var pubescens, V. articulatum and V.orientale using three different solvents viz., methanol, n-hexane and ethyl acetate. The antifungal activity was tested on three fungal strains include Fusarium oxysporum, Phytophthora infestans, Sclerotium rolfsii. Overall leaf extracts exerted better inhibitory activity than stem counterparts. Among all, only V. orientale was effective against the three fungal species.Compared to stem extracts, leaf extracts of D. falcata var pubescens have shown higher antifungal activity.

scholarly journals IDENTIFICATION OF ANTIBACTERIAL PROPERTY OF DIFFERENT MEDICINAL PLANT EXTRACTS

2020 ◽  
pp. 39-42
Author(s):  
SWETA KUMARI ◽  
VIRENDRA VAISHNAV
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
High Efficiency ◽  
Curcuma Longa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Cinnamomum Tamala ◽  
Medicinal Plant Extracts

Objective: The objective of this study was to evaluate the antibacterial activity of some plant extracts they are-Curcuma longa, Cinnamomum tamala, Ocimum tenuiflorum, Azadirachta indica, Datura stramonium, Zingiber officinale, Punica granatum and Cinnamomum cassia against Staphylococcus aureus and E. coli. Methods: The evaluation of antibacterial activity for different extracts of each plant was carried out by using the disc diffusion method and by pouring technique to determine the minimum inhibitory concentration (MIC). Results: In the disc diffusion method the plant extracts showed zone of inhibition ranging 02.0 to 16.0 mm against bacteria and In pouring technique plant extracts showed positive control against pure culture of a bacterium and their efficiency in terms of MICs where ranged from 0.2 gm/ml to 0.8 gm/ml. The extracts of Curcuma longa and Cinnamomum tamala shown high efficiency then other plant extracts in terms of zone of inhibition and also in pouring technique less amount of extracts was inhibited the growth of bacteria. Conclusion: This study indicates that extracts of these plants have antibacterial activity against bacteria. This report supports their use in the treatment of infections caused by bacteria.

In-vitro antioxidant and antimicrobial studies of ethanolic plant extracts of P. granatum, O. stamineus, A. bilimbi, M. nigra, and E. longifolia

2021 ◽  
Vol 22 ◽  
Author(s):  
Essam A. Makky ◽  
Manaf AlMatar ◽  
Mahmood H. Mahmood ◽  
Khoo Xu Wen ◽  
Tan Boon Gaik Qi
Keyword(s):  
Plant Extracts ◽  
Total Concentration ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Ethanolic Extracts ◽  
Mic Value

Background: Acne is a serious skin problem that affects mostly adolescents. The topical and systematic therapies are effective but could lead to several side effects and the emergence of antibacterial resistance of the acne-causing bacteria. Plant resources have been used as traditional medicine for centuries and can be the alternative therapies for acne treatment. Antioxidants are compounds that can prevent or delay the oxidation of substrates when present in low concentrations. Antioxidants are usually involved in several mechanisms of action, including the inhibition of free radical generation, enhancement of the scavenging capacity against free radicals, and reducing power. Methods: In this study, three antioxidant assays, DPPH, ABTS, and FRAP were used to evaluate the antioxidant properties of the ethanolic extracts of five plant extracts (A. bilimbi, M. nigra, O. stamineus, P. granatum, and E. longifolia). Furthermore, the study aimed to identify the most potent plant extracts and their combination which could provide better antibacterial activities against acne-causing bacteria. Ethanolic extracts of A. bilimbi, O. stamineus, M. nigra, P. granatum, and E. longifolia were prepared by the Ultrasound-Assisted Extraction (UAE) technique. Their phytochemical contents were screened using several biochemical tests and GC-MS analysis. Results: The study found that only the extracts of P. granatum and O. stamineus exhibited good antioxidant activity using DPPH assay (82.37% and 81.72% μg/mL respectively) and in ABTS assay (87.25% and 88.85% μg/mL respectively); their observed FRAP values were 115.1667 ± 5.6182 and 41.3860 ± 2.4583 μg/mL, respectively. The preliminary antibacterial screening using the disc diffusion method showed that P. granatum and O. stamineus were the most potent extracts; hence, both extracts were combined at the ratios of 1:1, 1:2, and 2:1 to a total concentration of 400 mg/mL. Discussion: The antibacterial efficacy of the mixture was studied using the disc diffusion method and the MIC value was determined. Both S. aureus and S. epidermidis were sensitive to all the individual and combined extracts but C. acnes was resistant to all of them. Conclusion: The antibacterial activity of the combined extracts against S. aureus showed a synergistic effect at the ratio of 2:1 with the inhibitory zone diameter of 18.00 ± 1.00 mm and MIC value of 12.5 mg/mL; however, antagonistic effects were observed against S. epidermidis while no effect was noted against C. acnes. Therefore, all the selected plant extracts exhibited antibacterial activities against certain bacteria and their effects may be enhanced by combining the plant extracts.

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals Antimicrobial Activity of Some Indigenous Plants of Bangladesh

1970 ◽  
Vol 7 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Mohammad S Rahman ◽  
Mohammed Z Rahman ◽  
Md Abdul Wahab ◽  
Rasheduzzaman Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Indigenous Plants ◽  
Gram Negative ◽  
Agar Disc ◽  
Bacteria And Fungi

The antimicrobial activity of methanol extracts of 17 plant species of Bangladesh was evaluated by the agar disc diffusion method. Among those, eight plant extracts exhibited potent antimicrobial activity against a wide variety of Gram positive and Gram negative bacteria and fungi at a concentration of 400 μg /disc.Key words: Antimicrobial activity, Disc diffusion method.DOI = 10.3329/dujps.v7i1.1213Dhaka Univ. J. Pharm. Sci. 7(1): 23-26, 2008 (June)

scholarly journals Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech Republic

2017 ◽  
Vol 62 (No. 7) ◽  
pp. 410-415 ◽  
Author(s):  
J. Bzdil ◽  
O. Holy ◽  
J. Toporcak
Keyword(s):  
Salmonella Enterica ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
The Czech Republic ◽  
Selective Enrichment ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Paratyphi B ◽  
Standard Disc

The aim of this study was to describe two case studies of reptile disease, in which the primary pathogen was apparently Salmonella enterica subspecies enterica serovar Paratyphi B. Pathological examinations, sampling from different organs and cloacal swabs was performed on a dead savannah monitor (Varanus exanthematicus) and a sick green tree python (Chondropython viridis). This material was subjected to culture examination, including selective enrichment using standard methods. Typing was performed using MALDI-TOF and strains were also serologically typified. The utilisation of d-tartrate was confirmed biochemically and also using PCR. Antibiotic susceptibility was determined by the standard disc diffusion method using Mueller-Hinton agar without blood and antibiotic discs. In both cases the detected Salmonella enterica subspecies enterica serovar Paratyphi B was positive for d-tartrate and exquisitely susceptible to chloramphenicol, tetracycline, ampicillin, amoxycillin/clavulanic acid, gentamicin, ceftazidime, enrofloxacin and piperacillin.

scholarly journals Combinations of herbs and probiotics as an alternative growth promoter: An in vitro study

2019 ◽  
Vol 12 (4) ◽  
pp. 614-620 ◽  
Author(s):  
Vinsa Cantya Prakasita ◽  
Widya Asmara ◽  
Sitarina Widyarini ◽  
Agnesia Endang Tri Hastuti Wahyuni
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Herbal Extract ◽  
Diffusion Method ◽  
Growth Promoter ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Ginger Extract ◽  
Adhesion Ability ◽  
Red Ginger

Background and Aim: Antibiotic growth promoters (AGPs) are added to animal feed to stimulate growth and increase livestock productivity. However, the regular use of antibiotics in animal diets has a considerable contribution to the occurrence of antibiotic resistance in livestock and humans. This study aimed to investigate the feasibility of red ginger (Zingiber officinale var. Rubrum), turmeric (Curcuma domestica), and wild ginger (Curcuma xanthorrhiza), Lactobacillus acidophilus, and Lactobacillus brevis as an alternative to AGPs. Materials and Methods: The antibacterial activities and probiotic stimulatory effects of herbs were screened through the disc diffusion method and optical densitometry. The inhibitory ability of probiotics against pathogens was also tested through the disc diffusion method. The adhesion ability of probiotics was tested by mixing the optimal herbal combinations with broiler intestinal epithelial cells (105 cells/ml). The cells were then subjected to Gram staining, and the number of adherent bacteria was calculated. Results: The test results showed that 3.13% ethanolic wild ginger extract had the highest inhibitory activity against Salmonella Enteritidis, followed by ethanolic red ginger extract and aqueous wild ginger extract at the same concentration. The three extracts also supported the growth of L. acidophilus and L. brevis. Further tests showed that the combination of 3.13% ethanolic red ginger extract had the highest inhibitory activity against S. Enteritidis, followed by ethanolic and aqueous wild ginger extract at the same concentration. The three extracts also supported the growth of L. acidophilus and L. brevis. Further tests showed that the combination of 3.13% ethanolic red ginger extract and 3.13% aqueous wild ginger extract had the best inhibitory effect on the growth of S. Enteritidis. The stimulatory effect of the combinations of herbal extract on the growth of L. acidophilus (0.18±0.00) and L. brevis (0.21±0.01) was better than those of individual extract, positive controls, and the glucose control. L. acidophilus and L. brevis had a weak inhibitory effect on the growth of S. Enteritidis (<6 mm). The adhesion ability of L. acidophilus (420.00±28.21) and L. brevis (259.33±24.03) was stronger than that of S. Enteritidis (202.00±14.00) under treatment with combined extracts. Conclusion: The tested combinations of herbs and probiotics can adhere to the intestinal tract. Given this characteristic, herb and probiotic combinations may be developed as an alternative to conventional AGPs.

scholarly journals Synergism of Zinc Oxide Nano-Powder with Active Compound from Turmeric and Lemongrass as Bacterial Inhibitor

2021 ◽  
Vol 18 (11) ◽  
Author(s):  
Gee Een LAU ◽  
Che Azurahanim CHE ABDULLAH ◽  
Mohd Sabri MOHD GHAZALI ◽  
Muhamad Syazlie CHE IBRAHIM
Keyword(s):  
Zinc Oxide ◽  
Plant Extracts ◽  
Zinc Oxide Nanoparticles ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Zones Of Inhibition

Green nanoparticles are receiving great attention due to their broad fields of application. Plant extracts are promising sources, since the synergizing process is easy and cost-effective. Green synergism offers safer nanomaterials for both human health and the environment. The antimicrobial application of prepared nanoparticles is due to special capabilities at the nanoscale size. Green zinc oxide nanoparticles (ZnONPs) from lemongrass and turmeric revealed good antibacterial properties. Antibacterial active compounds were extracted from lemongrass and turmeric using methanol as a solvent. The extracts were introduced to zinc oxide solution to create synergized green ZnONPs and tested for their antibacterial properties. The samples were characterized by means of XRD, SEM, FTIR, and GC-MS. The zones of inhibition of synergized green ZnONPs were successfully measured using the disc diffusion method. The findings using gram-negative bacteria Escherichia coli. showed a higher antibacterial inhibition zone, with a diameter of 1.6 cm. The gram-positive bacteria, Staphylococcus aureus., showed low antibacterial inhibition. The addition of ZnO had positively revealed greater inhibition. Green ZnONPs synthesized using plant extracts will be further tested for various applications. HIGHLIGHTS Green zinc oxide nanoparticles (ZnONPs) prepared from lemongrass and turmerics Plant extracts are significant in reducing and stabilizing metallic ions The synergism of ZnONPs was successfully prepared from plant extract active and evaluated for their antibacterial activity using Staphylococcus aureus and Escherichia coli The zones of inhibition of synergized green ZnONPs measured using the disc diffusion method showed a higher antibacterial inhibition zone Antibacterial activity revealed the potential applications of green synergism of ZnONPs for treatment of contaminated water, medical applications, and cosmetics GRAPHICAL ABSTRACT

scholarly journals Antibacterial activity of lemon, caraway and basil extracts on Listeria spp.

2012 ◽  
pp. 239-246
Author(s):  
Gordana Dimic ◽  
Suncica Kocic-Tanackov ◽  
Olivera Jovanov ◽  
Dragoljub Cvetkovic ◽  
Sinisa Markov ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Inhibitory Activity ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Inhibitory Zone ◽  
Disc Diffusion Method

Commercial extracts of three spices (lemon, caraway and basil) against Listeria monocytogenes, L. innocua and L. welshimeri were investigated using disc diffusion method. Lemon and basil extracts inhibited all the organisms at the level of ? 5 ?l (lemon) and 20 ?l (basil). These extracts produced inhibitory zones of 9-19 mm (lemon) and 8-11.5 mm (basil). The extract of caraway showed activity only against L. innocua at the highest level (20 ?l), producing an inhibitory zone of 14.7 mm. Generally, the lemon extract was the most effective. This extract exhibited greater inhibitory activity against L. monocytogenes, while the basil extract had the strongest effect on L. welshimeri.

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