scholarly journals EFICIÊNCIA DO USO DE NITROGÊNIO EM PLANTAS TRANSGÊNICAS DE TABACO COM ALTO ACÚMULO DE PROLINA

2018 ◽  
Vol 14 (4) ◽  
pp. 67-79
Author(s):  
Eliane Batista Viudes ◽  
Flaive Loyse Baldassarini Silva ◽  
Alessandra Ferreira Ribas ◽  
Luiz Gonzaga Esteves Vieira ◽  
Carlos Henrique Santos ◽  
...  
Keyword(s):  
Dry Mass ◽  
Proline Accumulation ◽  
Total Protein Content ◽  
High Accumulation ◽  
N Efficiency ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
And Control ◽  
Direct Use

Increasing the nitrogen use efficiencyofcrop plants is a result of the improvement in the mechanisms involved in nitrogen uptake, translocation, assimilation and remobilization. In this study, tobacco plants with high accumulation of proline caused by the insertion of the VaP5CSF129Amutant gene under control of the constitutive promoter CaMV35S was used as a model to investigate the effect of overexpression of the transgene in the uptake and utilization of N. For this, an experiment was carried out in a completely randomized design, in a 2x5 factorial arrangement, with two genotypes (transgenic eventand untransformed control) and five levels of nitrogen fertilization (25, 50, 100, 200 and 400 kg N ha-¹), cultivated under greenhouse conditions. Plants were evaluated for height, number of leaves, dry mass, free proline content, nitrogencontent, total protein content and use of the applied N (efficiency of absorption -EAbs, efficiency of leaf utilization –EUtiland efficiency of plant use -EUN). The transgenic plantswith high proline accumulation were less efficient than the control plants in terms of nitrogen absorption (with lowerN levelsin root, leaves and wholeplant), which resulted in lower EAbs. There was no difference between the transgenic and control genotypesfor EUtil. However, the transgenic plants presented lower EUN in comparison tothe control plants. Our data showed thattransgenictobacco plants withhigh proline accumulation do not have potential for direct use in agricultural systems, since the alteration of the biochemical balance between the metabolisms of nitrogen and carbon did not result in plants with higher EUN.

scholarly journals Integrated management of tough lovegrass (Eragrostis plana Nees): associating chemical control tools and plant physiology

2021 ◽  
Vol 51 (2) ◽  
Author(s):  
Eduardo Avelino Faleiro ◽  
Diego Martins Chiapinotto ◽  
Fabiane Pinto Lamego ◽  
Carlos Eduardo Schaedler ◽  
Eduardo Bohrer de Azevedo
Keyword(s):  
Solar Radiation ◽  
Integrated Management ◽  
Dry Mass ◽  
Radiation Factor ◽  
Factor B ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Factor C ◽  
Resource Conditions ◽  
And Control

ABSTRACT: The objective of the study was to evaluate the efficiency of herbicides use in the control of tough lovegrass according to the availability of solar radiation and the presence or absence of flooded. Two experiments were conducted in a completely randomized design, in a 2x2x4 and 2x2x5 factorial scheme, where factor A was equivalent to the environment (natural or reduced radiation); factor B to water condition (with a 2 cm flooded or without); and, factor C the herbicides: cyhalofop butyl (315 g ha-1), glyphosate (1080 g ha-1), cyhalofop butyl + glyphosate (315 g ha-1 + 1080 g ha-1) and control (without application) in the first experiment. In the second experiment, the herbicides used were cyhalofop butyl (315 g ha-1), glyphosate (1080 g ha-1), sethoxydim (184g ha-1), imazethapyr (106 g ha-1) and control (without application). Glyphosate efficiently controls plants of tough lovegrass with four tillers (>90%), being superior to cyhalofop, imazethapyr and sethoxydim; independently, of resource conditions. Reduction in the availability of solar radiation generates less shoot dry mass production from the weed, and improves the control only by imazethapyr and cyhalofop. In general, a flooded condition does not affect tough lovegrass control by herbicides.

scholarly journals EMISSÃO DE ÓXIDO NITROSO ASSOCIADO AO USO DE UREIA NO CULTIVO DE MELÃO AMARELO

Nativa ◽  
2018 ◽  
Vol 6 (6) ◽  
pp. 600
Author(s):  
Vanessa Cláudia Vasconcelos Segundo ◽  
Renato Innecco ◽  
Ebenézer De Oliveira Silva
Keyword(s):  
Leaf Area ◽  
Aerial Part ◽  
Dry Mass ◽  
Nitrogen Fertilizers ◽  
Root Part ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Brazilian Northeast ◽  
Total Soil Nitrogen

O meloeiro tem grande importância econômica para o Nordeste brasileiro. Entretanto, em seu cultivo utiliza-se insumos que podem prejudicar o ambiente; como, os fertilizantes nitrogenados. Objetivou-se avaliar a emissão de N2O em cultivo de meloeiro amarelo ‘Goldex’. O experimento foi instalado em delineamento inteiramente casualizado, com cinco repetições. Os tratamentos foram: 0 (controle), 1,0; 1,5 e 2 vezes a quantidade de nitrogênio recomendada para cada estádio. Foi analisado o fluxo de N2O, área foliar, número de folhas, massa seca da parte aérea, massa seca dos frutos, massa seca da parte radicular, nitrogênio total do solo, das plantas e N (NH4+ e NO3-) do solo. O fluxo de N2O teve comportamento temporal irregular durante a fase de plântulas e crescente na floração. Os tratamentos afetaram significativamente a área foliar, número de folhas, massa seca da parte aérea, massa seca da parte radicular, nitrogênio da parte aérea e parte radicular das plantas e do solo. A aplicação de nitrogênio, em dosagens superiores à recomendada, não aumentou a produção do meloeiro. E o cálculo do fator de emissão mostrou que a emissão do N2O não atingiu valores prejudiciais ao ambiente.Palavras-chave: fator de emissão, efeito estufa e nitrogênio. NITROUS OXIDE EMISSION ASSOCIATED WITH UREA USE THE YELLOW MELON CROP ABSTRACT: The melon has great economic importance for the Brazilian Northeast. However, in its cultivation uses inputs that can harm the environment; Such as nitrogen fertilizers. The objective of this study was to evaluate the emission of N2O in yellow 'Goldex' cultivar. The experiment was installed in a completely randomized design with five replications. The treatments were: 0 (control), 1.0; 1.5 and 2 times the amount of nitrogen recommended for each stage. N2O flux, leaf area, leaf number, shoot dry mass, fruit dry mass, dry mass of the root portion, total soil nitrogen, of the plants and N (NH4 + and NO3 -) of the soil were analyzed. The flow of N2O had irregular temporal behavior during the seedling phase and increased in flowering. The treatments affected significantly the leaf area, number of leaves, dry mass of the aerial part, dry mass of the root part, nitrogen of the aerial part and root part of the plants and the soil. The application of nitrogen, in dosages higher than the recommended one, did not increase the production of the melon. And the calculation of the emission factor showed that the emission of N2O did not reach values harmful to the environment.Keywords: emission fator, greenhouse and nitrogen.

scholarly journals Trichoderma Contributes to the Germination and Seedling Development of Açaí Palm

Agriculture ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 456
Author(s):  
Bruno Fróes Campos ◽  
Anselmo Junior Corrêa Araújo ◽  
Cristina Aledi Felsemburgh ◽  
Thiago Almeida Vieira ◽  
Denise Castro Lustosa
Keyword(s):  
Dry Mass ◽  
Initial Growth ◽  
Randomized Design ◽  
Trichoderma Isolate ◽  
Number Of Leaves ◽  
Collar Diameter ◽  
Completely Randomized Design ◽  
Set Up ◽  
Root Size ◽  
Trichoderma Isolates

The application of Trichoderma fungus can be used to obtain seedlings of higher quality for plantations. The objective of this study was to evaluate the effects of Trichoderma isolates on the germination and initial growth of açaí seedlings. Two trials were set up. In the laboratory, the seeds were inoculated in suspensions prepared with each of the isolates for 24 h, and they were arranged in trays containing filter paper in a completely randomized design with five replicates containing 50 seeds each. For the control, the seeds were submerged only in water. Part of the seedlings resulting from seeds treated and not treated with Trichoderma were transferred to bags containing forest soil and were submitted to seven modes of application of Trichoderma and kept in a nursery. The speed of germination and hypocotyl were not influenced by the biological treatment. The application of the different Trichoderma isolates positively influenced the height, collar diameter, number of leaves, root size, leaf area and root, and aerial part dry mass of the açaí seedlings. The application of the Trichoderma isolate TAM01 on the preplanting substrate and biweekly applications on the postplanting substrate was the combination that most positively influenced the development of açai seedlings.

scholarly journals Safflower genotypes submitted to soil density levels

2018 ◽  
Vol 9 (2) ◽  
pp. 142-153
Author(s):  
Edna Maria Bonfim-Silva ◽  
Juliana Tertezinha Sasso Paludo ◽  
Tonny José Araújo da Silva ◽  
William Fenner ◽  
Maurício Dutra Zanotto
Keyword(s):  
Soil Structure ◽  
Dry Mass ◽  
Carthamus Tinctorius ◽  
Soil Density ◽  
Randomized Design ◽  
Crop Development ◽  
Number Of Leaves ◽  
Agricultural Use ◽  
The Mean ◽  
Completely Randomized Design

It is essential to have knowledge of changes in soil structure, as a result of agricultural use and its relationship with crop development, especially those with potential for use in new regions, such as the cultivation of safflower (Carthamus tinctorius L.) in the Cerrado. In view of this dynamics, the objective was to evaluate the phytometric components of safflower genotypes submitted to levels of soil densities in Cerrado Oxisol. Ten safflower genotypes were then submitted to compaction (five densities: 1.0, 1, 2, 1.4, and 1.8 Mg m-3) in a completely randomized design with four replications under greenhouse conditions. The plots consisted of an overlap of three rings of polyvinylidene chloride of 0.01 m height each, and the treatments were applied in the intermediate layer. The phytometric components (plant height, number of leaves and stem diameter) and shoot dry mass of the crop were evaluated at 30, 60 and 90 days after sowing. There was no significant interaction between safflower genotypes and soil densities, with the effects being isolated for genotypes and/or densities when significant. For the phytometric characteristics evaluated, the genotypes PI250196, PI301049, PI305173, and PI305205 are more stable about the variations of soil density in comparison to the others. The phytometric components and dry mass of safflower shoot are impaired from the mean density of 1.2 Mg m-3.

scholarly journals Ruellia simplex cuttings propagation produced on carnauba waste substrate

2020 ◽  
Vol 14 ◽  
Author(s):  
Taciella Fernandes Silva ◽  
Klayton Antonio Do Lago Lopes ◽  
Janaiane Ferreira Dos Santos ◽  
Ana Paula De Almeida Sousa ◽  
Francisca Gislene Albano-Machado ◽  
...  
Keyword(s):  
Root System ◽  
Aerial Part ◽  
Ornamental Plants ◽  
Dry Mass ◽  
Fresh Mass ◽  
Randomized Design ◽  
Shoot Number ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design

The production of quality seedlings is necessary for the trading of ornamental plants. Seedling quality depends on several factors, among these factors, the substrate is highlighted. Thus, this study aimed to evaluate the effect of substrates based on bagana of carnauba, in the production of Ruellia simplex seedlings, by vegetative propagation. A completely randomized design was adopted, with six treatments and four replications. The treatments (substrates) were composed of bagana of carnauba (BC) and soil, in the following proportions: BC0 - 0% BC + 100% of soil; BC20 - 20% BC + 80% of soil; BC40 - 40% BC + 60% of soil; BC60 - 60% BC + 40% of soil; BC80 - 80% BC + 20% of soil; BC100 - 100% BC + 0% of soil. The plot consisted of a plant. After 45 days of cutting, it was evaluated: number of shoots; number of leaves per shoot; diameter of the highest shoot; length of the highest shoot; root length; inflorescence number; number of flowers per inflorescence; leaf area; root volume; aerial part fresh mass; root system fresh mass; aerial part dry mass; and root system dry mass. There was a significant difference for the variables, number of leaves per shoot, diameter and length of the highest shoot, number of flowers per inflorescence, fresh and dry mass of the aerial part. Having a direct relationship between the increase in the proportion of bagana of carnauba volume and the inflorescence number, the substrate 100% of bagana of carnauba is indicated.

scholarly journals Production of papaya seedlings and initial growth produced in different polyethylene recipients

2021 ◽  
Vol 14 (8) ◽  
Author(s):  
M. Marcolin ◽  
J. A. Massaroto ◽  
R. N. Souza ◽  
R. R. Rocha
Keyword(s):  
Dry Weight ◽  
Dry Mass ◽  
Initial Growth ◽  
State University ◽  
Mato Grosso ◽  
Randomized Design ◽  
Green Matter ◽  
Number Of Leaves ◽  
Polyethylene Bags ◽  
Completely Randomized Design

The objective of this study was to evaluate the vegetative and productive performance of papaya seedlings in response to different volumes of containers. The experiment was conducted in a seedling production nursery at the State University of Mato Grosso (UNEMAT), Campus de Nova Mutum - MT, using a completely randomized design with five replications, in which four sizes of polyethylene bags were tested in the production of seedlings: 10 x 16 cm, 15 x 21 cm, 17 x 27 cm and 20 x 21 The plots were composed of 3 plants. The parameters evaluated at 70 days after sowing were: plant height, stem diameter, number of leaves, root length, green matter mass and percentage of dry weight of the stem, leaves and roots. The container 17 x 27 cm stood out from the other containers, except for the characteristics of the length of the stem, percentage of dry mass of the stem and leaves where it was equivalent to the container 20 x 21 cm and the percentage of dry mass where all containers were equivalent. The experiment showed that the 17 x 27 cm container is the most recommended for the cultivation of papaya seedlings cultivar Sunrise Solo.

scholarly journals Proline synthesis and physiological response of cassava genotypes under in vitro salinity

2019 ◽  
Vol 49 (6) ◽  
Author(s):  
Milena Nascimento Cardoso ◽  
Aparecida Gomes de Araújo ◽  
Leila Albuquerque Resende Oliveira ◽  
Bruno Trindade Cardoso ◽  
Ana Veruska Cruz da Silva Muniz ◽  
...  
Keyword(s):  
Salt Stress ◽  
Physiological Response ◽  
Dry Mass ◽  
Proline Content ◽  
Randomized Design ◽  
Regression Test ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Double Factorial

ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro.

Effect of hormone on the seed germination of Garcinia kola Heckel.

2015 ◽  
Vol 11 (2) ◽  
pp. 223-231
Author(s):  
FB Yakubu ◽  
JO Ogunade ◽  
OT Bolanle-Ojo ◽  
DK Yahaya
Keyword(s):  
Seed Germination ◽  
Early Growth ◽  
Garcinia Kola ◽  
Randomized Design ◽  
In The Wild ◽  
Number Of Leaves ◽  
Collar Diameter ◽  
Completely Randomized Design ◽  
Garcinia Kola Heckel ◽  
And Control

Garcinia kola plays an important socioeconomic role in the Africa culture. Inspite of this, the plant only exists in the wild or as part of left over in the Agroforestry farms. Therefore, this research examined the phenology in seed germination for possible plantation establishment. G. kola seed dormancy and growth was investigated using hormone Napthalene Acetic Acid (NAA) and Benzene Amino Purine (BAP) application to induce quick germination and early growth The experiment was laid in a Completely Randomized Design (CRD) with seven treatments (0.5mg/ml BAP, 0.5mg/ml NAA, 1mg/ml BAP, 1mg/ml NAA, 2mg/ml BAP, 2mg/ml NAA and Control) in twenty replications. The results showed that Garcinia kola seeds germinated in seven weeks in IBA. Growth were assessed for 19wks and the result showed that Length of Radicle (LR) and Number of Radicle (NR) were significantly affected by the treatments, while 0.5mg/ml IBA gave the best result among the treatments. Length of Plumule, Number of Leaves and Collar diameter were not significantly affected by the treatments.Keywords: Garcinia kola, seed, hormone, dormancy, germination

scholarly journals Nitrogen, potassium, calcium and sulfur omission in grass Convert

2018 ◽  
Vol 8 (3) ◽  
pp. 452-458
Author(s):  
Rafaela Pereira Miranda ◽  
Marizane Pietroski ◽  
Fabiano Bernardo Matos ◽  
Getulio Freitas Seben Junior ◽  
Gustavo Caione
Keyword(s):  
Nutrient Solution ◽  
Solution Treatment ◽  
Dry Mass ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Root Size ◽  
Mass Accumulation

Intensive use of Brazilian pastures without the maintenance of soil fertility has decreased their productivity and favoring the onset of symptoms of nutritional deficiency. The objective of this study was to evaluate the effect of nitrogen, potassium, calcium and sulfur omission in symptomatology, growth and dry mass accumulation in grass Convert. The experiment was conducted in a greenhouse with a completely randomized design, with five treatments and four replications. The treatments were: full nutrient solution, nitrogen omission (N), potassium omission (K), calcium omission (-Ca) and sulfur omission (S). The variables were the number of tillers, leaf number, root size, shoot height, characterization of symptoms of deficiency and shoot and root dry mass accumulation. The data were submitted to the F test by analysis of variance and comparison of means by Tukey’s test (p <0.05). The omission of N, K, Ca and S limited the development of Convert grass compared to the full nutrient solution treatment, reducing the number of leaves, tillers, height, root size and dry mass production. The nutrient omission modified forage morphology, showing visual symptoms of deficiency of each nutrient evaluated. The decreasing order of onset of symptoms was N, Ca, S and K.

scholarly journals Growth variation and proline accumulation of Echinacea purpurea cultivated to CaCl2 salinity

2021 ◽  
Vol 905 (1) ◽  
pp. 012017
Author(s):  
J P Choirunnisa ◽  
Y Widiyastuti ◽  
B Pujiasmanto ◽  
A T Sakya ◽  
A Yunus
Keyword(s):  
Medicinal Plant ◽  
Echinacea Purpurea ◽  
Proline Accumulation ◽  
Purple Coneflower ◽  
Randomized Design ◽  
Growth Variation ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Saline Land ◽  
Number Of Branches

Abstract Purple coneflower (Echinacea purpurea (L.) Moench) is classified as medicinal plant comes from North America and not much developed in Indonesia. E. purpurea can be developed by utilizing suboptimal land such as saline land. This study aims to determine the effect of CaCl2 concentration on growth and proline accumulation of three accessions E. purpurea. This study used a Completely Randomized Design (CRD) with 2 factors and five replications. The first factor is three accessions E. purpurea (accession 1; 2; and 3). The two factor is four concentrations CaCl2 (0; 2500; 5000; and 10000 ppm). The observations are plant height, number of leaves, number of branches and proline accumulation. Data were analyzed using SPSS to test difference. The results showed that highest concentration of CaCl2 can inhibit growth and increase proline accumulation. The highest proline accumulation in accession 2 was 22.8002 µmol g−1, accession 2 as an indicator of accession tolerant to salinity.

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