scholarly journals Antimicrobial Efficacy of Biogenic Silver and Zinc Nanocrystals/Nanoparticles to Combat the Drug Resistance in Human Pathogens

2021 ◽  
Author(s):  
Gulzar Ahmed Rather ◽  
Saqib Hassan ◽  
Surajit Pal ◽  
Mohd Hashim Khan ◽  
Heshu Sulaiman Rahman ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Active Surface ◽  
Multi Drug Resistance ◽  
Human Pathogens ◽  
New Paradigm ◽  
Antimicrobial Drugs ◽  
Innovative Strategies ◽  
Health Organization ◽  
Conventional Antibiotics ◽  
Biogenic Nanoparticles

The emergence of biogenic nanomaterials as novel antimicrobials introduces a new paradigm in human health care. Based on the recent reports of the World Health Organization, infectious diseases pose one of the greatest health challenges. Increased multi-drug resistance prevalence among human pathogens, due to the inefficiency of commercially available antimicrobial drugs in the market is a great threat to humans. The poor solubility, stability and side effects of the antibacterial therapy prompted the researchers to explore new innovative strategies for developing new antimicrobials. Recently, biogenic nanoparticles have proven their effectiveness against multidrug-resistant (MDR) pathogens as an alternative to conventional antibiotics. Biogenic nanoparticles such as silver nanoparticles (AgNPs) and Zinc Oxide nanoparticles (ZnONPs) are easy to produce, biocompatible, provide enhanced uptake and are eco-friendly. Moreover, the capping of the biogenic nanocrystals provides an active surface for interaction with biological components, facilitated by free active surface functional groups to enhance their efficacy and delivery. Inorganic nanocrystals (AgNPs and ZnONPs) are effective both as nano-bactericides and as nanocarriers against sensitive and MDR) pathogens. The present chapter focuses on the utilization of the recent nanosystems to combat drug resistance in human pathogens. Nanomedicine represents a new generation of potiential antimicrobial candidates capable of combating the drug resistance in various pathogenic organisms.

scholarly journals Antimicrobial Effects of Biogenic Nanoparticles

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1009 ◽  
Author(s):  
Priyanka Singh ◽  
Abhroop Garg ◽  
Santosh Pandit ◽  
V. Mokkapati ◽  
Ivan Mijakovic
Keyword(s):  
Infectious Diseases ◽  
Metallic Nanoparticles ◽  
Active Surface ◽  
Multidrug Resistant ◽  
Antimicrobial Drugs ◽  
Long Term Stability ◽  
New Antibiotics ◽  
Conventional Antibiotics ◽  
Biogenic Nanoparticles

Infectious diseases pose one of the greatest health challenges in the medical world. Though numerous antimicrobial drugs are commercially available, they often lack effectiveness against recently developed multidrug resistant (MDR) microorganisms. This results in high antibiotic dose administration and a need to develop new antibiotics, which in turn requires time, money, and labor investments. Recently, biogenic metallic nanoparticles have proven their effectiveness against MDR microorganisms, individually and in synergy with the current/conventional antibiotics. Importantly, biogenic nanoparticles are easy to produce, facile, biocompatible, and environmentally friendly in nature. In addition, biogenic nanoparticles are surrounded by capping layers, which provide them with biocompatibility and long-term stability. Moreover, these capping layers provide an active surface for interaction with biological components, facilitated by free active surface functional groups. These groups are available for modification, such as conjugation with antimicrobial drugs, genes, and peptides, in order to enhance their efficacy and delivery. This review summarizes the conventional antibiotic treatments and highlights the benefits of using nanoparticles in combating infectious diseases.

Effect of long non-coding RNA EIF3J-AS1 on multi-drug resistance and autophagy in gastric cancer.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e15581-e15581
Author(s):  
Yuhao Luo ◽  
Rui Zhou ◽  
Na Huang ◽  
Li Sun ◽  
Wangjun Liao
Keyword(s):  
Gastric Cancer ◽  
Drug Resistance ◽  
Treatment Options ◽  
Cancer Therapeutics ◽  
Underlying Mechanism ◽  
Multi Drug Resistance ◽  
New Paradigm ◽  
Gastric Cancer Cells ◽  
Non Coding Rna ◽  
Long Non Coding Rna

e15581 Background: Gastric cancer (GC) is a leading cause of cancer mortality worldwide, oxaliplatin and epirubicin based chemotherapy are one of the most important treatment options for GC patients. However, drug resistance, especially multi-drug resistance remains a major obstacle for successful chemotherapy. Recently, long non-coding RNAs (lncRNAs) have been widely identified to play emerging roles in diverse physiological and pathophysiological processes including drug resistance. Our previous bioinformatics analysis showed long non-coding RNA EIF3J-AS1 was a potential multi-drug resistance gene, but the underlying mechanism is still unknown. Methods: We generated oxaliplatin resistance cells (MGC803/OXA) and epirubicin resistance cells(MGC803/EPI) based on parental gastric cancer cells MGC803. Relative expression levels of EIF3J-AS1 were measured by qRT-PCR. Transmission electron microscopy was used to measure autophagosomes. Rapamycin was applied to inducing autophagy while chloroquine and 3-methyladenine were used to block autophagy. Protein level of autophagy related genes were examined by Western Blot. Coexpression genes of EIF3J-AS1 from TCGA RNA-seq datas were analyzed by cBiportal. RNA immunoprecipitation was used to analyze endogenous microRNAs and mRNAs. Results: EIF3J-AS1 was significantly upregulated in MGC803/OXA and MGC803/EPI cells compared with parental cells MGC803. EIF3J-AS1 inhibition increased chemosensitivity to both oxaliplatin and epirubicin. Moreover, EIF3J-AS1 silence lead to the decrease of autophagy. Autophagy related gene ATG14 was identified as a downstream target gene. EIF3J-AS1 promoted ATG14 expression by directly interacting with and increasing stability of ATG14 mRNA, On the other hand, EIF3J-AS1 competitively sponged miR-188-3p and promoted ATG14 expression in a ceRNA-dependent way. Conclusions: LncRNA EIF3J-AS1 is a crucial regulator of multi-drug resistance by inducing autophagy in gastric cancer. Targeting EIF3J-AS1/ATG14 axis might be a new paradigm for cancer therapeutics.

scholarly journals Trend and pattern of antimicrobial resistance in molluscanVibriospecies sourced to Canadian estuaries

2018 ◽  
Author(s):  
Swapan K. Banerjee ◽  
Jeffrey M. Farber
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Resistance ◽  
Health Hazard ◽  
Surveillance Study ◽  
Health Concern ◽  
Multi Drug Resistance ◽  
Seafood Consumption ◽  
Human Pathogens ◽  
Potential Health

ABSTRACTEmergence of antimicrobial resistance (AMR) in foodborne bacteria is a growing concern worldwide. AMR surveillance is a key element in understanding the implications resulting from the use of antibiotics for therapeutic as well as prophylactic needs. The emergence and spread of AMR in foodborne human pathogens is an indirect health hazard. This surveillance study reports the trend and pattern of AMR detected inVibriospecies isolated from molluscs harvested in Canada, between 2006 and 2012, against 19 commonly used antibiotics. Five common antibiotics, ampicillin, cephalothin, erythromycin, kanamycin and streptomycin, predominantly contributed to AMR including multi-drug resistance (MDR) in the molluscanVibriospp. isolated in 2006. A prospective follow-up analysis of these drugs showed a declining trend in the frequency of MDR/AMR-Vibriospp. in subsequent years until 2012. The observed decline appears to have been influenced by the specific downturn in resistance to the aminoglycosides, kanamycin and streptomycin. Frequently observed MDR/AMR-Vibriospp. in seafood is a potential health concern associated with seafood consumption. Our surveillance study provided an indication of the antibiotics that challenged the marine bacteria, sourced to Canadian estuaries, during and/or prior to the study period.

scholarly journals Antimicrobial Resistance in Staphylococcus aureus

2021 ◽  
Author(s):  
Riya Mukherjee ◽  
Anjali Priyadarshini ◽  
Ramendra Pati Pandey ◽  
Vethakkani Samuel Raj
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Resistance ◽  
Antimicrobial Agents ◽  
Multi Drug Resistance ◽  
Antimicrobial Drugs ◽  
Mortality And Morbidity ◽  
Gradual Development ◽  
Resistance Patterns ◽  
Resistant Strains ◽  
Mrsa Strains

Staphylococcus aureus is a Gram-Positive bacteria that are responsible to cause skin infections and also shows toxic shock syndrome. Several antibiotics were given against the S. aureus infections but eventually, the prevalence of multidrug resistance of Staphylococcus aureus started emerging. Since then Methicillin-resistant Staphylococcus aureus strains (MRSA)were very common which causes nosocomial infections. Microorganisms for the need of the survival undergoes mutational changes either in their chromosomal DNA/RNA which confers the resistance. One of the famous examples is the resistance against methicillin in Staphylococcus aureus. The evolution of S. aureus is successful in developing multiple resistant strains. Plasmids are capable of carrying the resistant genes and also several toxic genes. In a recent study, it has been observed that drug resistance genes are located in the R plasmids and they are also responsible in conferring multi drug resistance and induce less utilization of multiple antimicrobial therapy. MRSA was not only resistant to methicillin, studies proved MRSA strains were resistant to macrolides, tetracyclines, chloramphenicol. Resistance to vancomycin was very evidently observed, and its transfer among the population and rising of resistant strains was becoming a major threat globally. The resistance of all these antimicrobial agents against the pathogenic microorganisms are taking a rise in some patients due to prolong use of the antimicrobial agents by these patients. The multi drug resistance has enhanced the mortality and morbidity rate which referred to the infecting agents as the “Super Bugs”. Survival of the microorganisms has increased due to the gradual development of extensive resistance against varied antimicrobial drugs. Possible treatments with combinations are found to be the only hope for infections against S. aureus. Few drugs are in development such as Dalbavancin, Oritavancin, Tigecycline. These are the possible treatments upon which the work is going on to reduce the resistance against the invasive MRSA. This chapter highlights the profiles of Staphylococcus aureus and the resistance patterns along with transmission and the role of the plasmid in transmitting the resistance.

scholarly journals ROLE OF MEDICINAL PLANT EXTRACTS IN ERADICATION OF MULTI DRUG RESISTANT MICROBES

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Smruti Mahapatra ◽  
Pratyush Kumar Das ◽  
Gopal Krishna Purohit ◽  
Sangram Pattanaik ◽  
Swadhin Palo
Keyword(s):  
Drug Resistance ◽  
Significant Role ◽  
Folk Medicine ◽  
Herbal Medicines ◽  
Multi Drug Resistance ◽  
Product Lines ◽  
Antimicrobial Drugs ◽  
Medicinal Plant Extracts ◽  
Solid Foundation

The Indian Ayurvedic medicine is a classical medicinal method that has a rich history and solid foundation. Herbal medicines play a significant role in the prevention and management of human diseases. For millennia, plants have been used as folk medicine. Medicinal plants are an abundant source of the secondary metabolite formed by plants, the bioactive phytochemicals. Scientific studies in recent decades have demonstrated the significant role of these phytochemicals in the prevention of infectious disease. In recent decades, the re-discovery of medicative plants as a source of potential therapies has become a great deal of interest. Plant-based product lines can regulate microbial development in different scenarios. The worldwide danger to public health arose from the resistance among distinct microbial species to multiple antimicrobial drugs. Approximately every competent infective agent has high levels of multi drug resistance with strengthened morbidity & mortality, which makes them called 'superbugs.' Only by designing entirely new methods, the multi-drug resistance crisis can be tackled. The goal of this study is true to clarify the information of medicinal herbs as a potential basis for herbal supplements against microbes with multi-drug resistance.

scholarly journals Investigation into infectability and antimicrobial susceptibility of Salmonella spp. isolated from fresh seafood at traditional markets in Ho Chi Minh city

2021 ◽  
Vol 4 (1) ◽  
pp. 18-20
Author(s):  
Vu Truong Huynh Anh ◽  
Khue Tu Nguyen Hoang ◽  
Hai Chu Van ◽  
Ha Huynh Yen ◽  
◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Antimicrobial Susceptibility ◽  
Traditional Method ◽  
Ho Chi Minh City ◽  
Multi Drug Resistance ◽  
Salmonella Spp ◽  
Antimicrobial Drugs ◽  
Traditional Markets ◽  
Fish Samples

In this study, a total of 380 fresh seafood samples (fish, shrimp, squid) were collected randomly at conventional markets of districts in Ho Chi Minh city. Salmonella strains were detected by the traditional method (ISO 6579-1:2017) and conformed by PCR technique (TCVN 8342:2010), serotyping according to ISO/TR 6579-3:2014 and Kirby-Bauer methods evaluated antibiotic resistance's ability. As a result, 85 Salmonella strains were isolated, and the proportion of infection was 22,37% (85/380). The proportion of Salmonella strains that resisted 01 antibiotics was 85,88% (73/85), and 10,59% (09/85) accounted for that of strains that resisted 02 to 05 antibiotics. Also, strains representing resistance towards 06 to 11 antibiotics occupied 4,71% (04/85). Antimicrobial drugs resisted the most were tetracycline 43, 53% (37/85). In contrast, 98,82% (84/85) of Salmonella strains were sensitive to ceftazidime. The proportion of multi-drug resistance was 15,29% (13/85). The familiar combinations of antibiotic resistance were ampicillin, streptomycin, tetracycline, and trimethoprim/sulfamethoxazole (AM, STR, TE, SXT), with 46,15% (6/13). There were six distinguished serovars, including S. Kentucky (05 strains); S. Infantis (02 strains); S. Agona and S. Postdam (01 strain); S. Saintpaul, S. Braenderup (01 strain). 92.31% of serovar detected resistance genes (blaTEM, strA: 53.85%; blaSHV: 7.69%; tetA: 92.31%; tetB: 7.69% and sul1: 23.08%). Three serovars with genotype (blaTEM, strA, tetA, sul1) matched the antibiotic resistance phenotype (AM, STR, TE, SXT), namely S. Kentucky (02) and S. Saintpaul (01), the both isolated from fish samples.

The gut microbiota resistome provides development of drug resistance in causative agents of human infectious diseases

2017 ◽  
pp. 20-32 ◽  
Author(s):  
Е.Н. Ильина ◽  
Е.И. Олехнович ◽  
А.В. Павленко
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Integrated Approach ◽  
Modern Medicine ◽  
Pathogenic Microorganisms ◽  
Human Pathogens ◽  
Potential Source ◽  
Causative Agents ◽  
Bacterial Genes

С течением времени подходы к изучению резистентности к антибиотикам трансформировались от сосредоточения на выделенных в виде чистой культуры патогенных микроорганизмах к исследованию резистентности на уровне микробных сообществ, составляющих биотопы человека и окружающей среды. По мере того, как продвигается изучение устойчивости к антибиотикам, возникает необходимость использования комплексного подхода для улучшения информирования мирового сообщества о наблюдаемых тенденциях в этой области. Все более очевидным становится то, что, хотя не все гены резистентности могут географически и филогенетически распространяться, угроза, которую они представляют, действительно серьезная и требует комплексных междисциплинарных исследований. В настоящее время резистентность к антибиотикам среди патогенов человека стала основной угрозой в современной медицине, и существует значительный интерес к определению ниши, в которых бактерии могут получить гены антибиотикорезистентности, и механизмов их передачи. В данном обзоре мы рассматриваем проблемы, возникшие на фоне широкого использования человечеством антибактериальных препаратов, в свете формирования микрофлорой кишечника резервуара генов резистентности. Over the time, studies of antibiotic resistance have transformed from focusing on pathogenic microorganisms isolated as a pure culture to analysis of resistance at the level of microbial communities that constitute human and environmental biotopes. Advancing studies of antibiotic resistance require an integrated approach to enhance availability of information about observed tendencies in this field to the global community. It becomes increasingly obvious that, even though not all resistance genes can geographically and phylogenetically spread, the threat they pose is indeed serious and requires complex interdisciplinary research. Currently, the antibiotic resistance of human pathogens has become a challenge to modern medicine, which is now focusing on determining a potential source for bacterial genes of drug resistance and mechanisms for the gene transmission. In this review, we discussed problems generated by the widespread use of antibacterial drugs in the light of forming a reservoir of resistance genes by gut microflora.

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