scholarly journals High Levels of Circulating IL-6 and IL-8 Signature can Predict COVID-19 Severity

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Didem Ozgür ◽  
Murat Karamese ◽  
Alpay Medetalibeyoglu ◽  
Onur Alkan ◽  
Naci Senkal ◽  
...  
Keyword(s):  
Serum Levels ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Cytokines And Chemokines ◽  
Tnf Α ◽  
Severe Group ◽  
Ifn Γ ◽  
The Mean ◽  
Circulating Levels ◽  
Human Cytokine

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may trigger a cytokine storm, which is characterized by uncontrolled overproduction of proinflammatory cytokines. Objectives: We aimed to investigate the association between circulating levels of inflammatory cytokines and severity of coronavirus disease 2019 (COVID-19). Methods: This cross-sectional study included 46 severe and 32 mildly symptomatic COVID-19 patients. The serum levels of cytokines and chemokines were determined using the Bio-Plex ProTM Human Cytokine Screening Panel. Results: Out of a total of 78 patients with confirmed COVID-19, 54 (69.2%) were males, and 24 (30.8%) were females. The mean age was 43.1 ± 13.3 and 58.2 ± 15 in mild and severe patients, respectively. Severe patients were characterized by significant laboratory abnormalities, such as increased WBC (P = 0.002) and neutrophil counts (P = 0.001), higher levels of ALT (P = 0.03), AST (P = 0.002), LDH (P < 0.001), urea (P = 0.013), ferritin (P < 0.001), D-dimer (P = 0.042), CRP (P < 0.001), and decreased lymphocyte (P < 0.001) and platelet (P = 0.045) counts. The levels of IL-6, IL-8, IL-13, TNF-α, IFN-γ, MIP-1β, and MCP-1 increased in the severe group compared to the mild group. However, significant differences were observed only for IL-6 (P < 0.001) and IL-8 (P < 0.001) levels. Conclusions: Serum IL-6 and IL-8 levels can be used as potential prognostic biomarkers of disease severity in COVID-19 patients.

scholarly journals Correlation between Circulating Levels of Pro-Inflammatory Cytokines TNF-alpha and Vascular Calcification Inhibitor Matrix Gla Protein in Obese Men

2010 ◽  
Vol 2 (3) ◽  
pp. 126
Author(s):  
Trilis Yulianti ◽  
Mansyur Arif ◽  
Andi Wijaya
Keyword(s):  
Vascular Calcification ◽  
Inflammatory Cytokines ◽  
Univariate Analysis ◽  
Cross Sectional Study ◽  
Matrix Gla Protein ◽  
Sectional Study ◽  
Cross Sectional ◽  
Tnf Α ◽  
Circulating Levels ◽  
Pro Inflammatory Cytokines

BACKGROUND: Adult obesity is rapidly increasing in the world including Indonesia. Tumor necrosis factor α (TNF-α) was chronically elevated in obese adipose tissue. TNF-α, a pleiotropic cytokine and also a regulator of bone formation, may might represent an important link between obesity and vascular calcification. Elegant genetic studies in mice and human have highlighted the important roles for Matrix Gla Protein (MGP) as an inhibitor of vascular calcification. The aim of this study was to examine the correlation between circulating levels of pro-inflammatory cytokines TNF-α and vascular calcification inhibitor MGP in obese men.METHODS: This was an observational cross-sectional study including 40 central obese men (waist circumference ≥90 cm) aged 31-60 years old. Serum MGP and serum TNF-α concentrations were quantified by ELISA principle. Fasting plasma glucose was assessed using hexokinase methods, triglyceride by GPO-PAP methods, and creatinine by Jaffe methods. All assays were performed according to the manufacture instruction. Statistical analysis was performed with SPSS for windows ver 16. Univariate analysis were performed to analyze mean, maximum, minimum value and SD. Pearson correlation statistic were performed to determine the correlation between variables. Significance value were define as alpha level = 0.05 based on two-tailed tests.RESULTS: The cross-sectional study (n=40) showed that the advancing age was correlated with plasma TNF-α concentration (r=0.348; p=0.028). The mean concentration of TNF-α and MGP were 8.323 and 8.368, respectively. We found a significant negative correlation between TNF-α with MGP (r=-0.425; p=0.006) and a significant correlation between TNF-α and triglyceride (r=0.375; p=0.017).CONCLUSIONS: Circulating level of TNF-α was inversely correlated with MGP concentration in obese men. This finding suggested that high level TNF-α leads to low MGP concentration obese men, hence, limits inhibitory capacity in vascular calcification.KEYWORDS: hypertension, obesity, vascular calcification, MGP, TNF-α

scholarly journals Hypovitaminosis D Is Associated with Higher Levels of Inflammatory Cytokines and with HAM/TSP in HTLV-Infected Patients

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2223
Author(s):  
Elaine Coutinho Netto ◽  
Alfredo Carlos Silva ◽  
Célia Pedroso ◽  
Carlos Brites
Keyword(s):  
Vitamin D ◽  
Spastic Paraparesis ◽  
Hypovitaminosis D ◽  
Cross Sectional Study ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
Asymptomatic Carriers ◽  
Tnf Α ◽  
Ifn Γ ◽  
And Gender

Recent studies have shown the effects of vitamin D on host response to infectious diseases. Some studies detected a high prevalence of hypovitaminosis D in HIV-infected patients, but scarce information exists for HTLV-1 infection. We conducted a cross-sectional study to evaluate the frequency of hypovitaminosis D in HTLV-1 patients and its relationship with their immune response in HTLV-infected patients and in age- and gender-matched controls at a Brazilian rehabilitation hospital. We compared vitamin D, interleukin-6 (IL-6), tumoral necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) levels across groups. Logistic regression was utilized to assess the association between hypovitaminosis D and cytokine levels. We enrolled 161 HTLV-infected subjects (129 HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients, 32 asymptomatic HTLV carriers) and equal number of HTLV-negative controls. We observed a significantly higher prevalence of hypovitaminosis D in patients with HAM/TSP than in HTLV asymptomatic carriers (p < 0.001), or controls (p < 0.001). HAM/TSP patients also had higher levels of IL-6 and IFN-γ than asymptomatic carriers. Patients with HAM/TSP and hypovitaminosis D had higher levels of TNF-α than asymptomatic HTLV carriers. These findings suggest hypovitaminosis D plays a role in HAM/TSP pathogenesis, and it needs to be evaluated in further studies.

scholarly journals PERBEDAAN RERATA KADAR AKTIVIN A SERUM MATERNAL ANTARA PREEKLAMSIA BERAT DENGAN BUKAN PREEKLAMSIA BERAT

2019 ◽  
Vol 1 (1) ◽  
pp. 30-37
Author(s):  
Dovy Djanas ◽  
Bayu Pramudyo Ariwibowo ◽  
Hafni Bachtiar
Keyword(s):  
Serum Levels ◽  
Activin A ◽  
Maternal Serum ◽  
Severe Preeclampsia ◽  
Inclusion Criteria ◽  
Exclusion Criteria ◽  
Cross Sectional ◽  
Tnf Α ◽  
The Mean ◽  
In Pregnancy

At the start of preelampsia there is a failure of cytotrophoblst invasion into the maternal spiral arteries that will lead to decreased uteroplacetal perfusion which will be followed by the failure of the unit fetoplacenter to get enough oxygen from the room intervillous that ultimately lead to a state of hypoxia in placenta. This will cause the expenditure of TNF-α dan IL-1β from placenta and a factors called hypoxia-inducible transcription factors that will spur the trophoblast to produce activin A lot more. This research was conducted by cross sectional method in maternal room of obstetrics and gynecology department of Central General Hospital of Dr. M. Djamil Padang from August 2015 until February 2016 with 20 patients of severe preeclampsia and 20 patients not severe preeclampsia, who met inclusion criteria and there is no exclusion criteria. Then performed statistical analysis using Mann-Whitney test to determine difference in mean maternal activin A serum levels of severe preeclampsia and not severe preeclampsia. The mean maternal serum levels of activin A in severe preeclampsia is 32,55 ± 1,84 ng/ml and in pregnancy with no severe preeclampsia is 8,59 ± 0,59 ng/ ml. Difference in mean maternal serum level of activin A in the two groups was statistically significant (p=0,001). Ma-ternal serum activin A levels is significantly higher in severe preeclampsia than pregnancy with no severe preeclampsia.Keywords: Activin A, severe preeclampsia, not severe preeclampsia

Variation in the Level of Thyroid Markers in Association with Inflammation in Patients with Type 2 Diabetes

2020 ◽  
Vol 20 (6) ◽  
pp. 924-929
Author(s):  
Khalid Siddiqui ◽  
Teena P. George ◽  
Salini Scaria Joy ◽  
Muhammad Mujammami
Keyword(s):  
Type 2 Diabetes ◽  
Glucose Metabolism ◽  
Thyroid Hormones ◽  
Inflammatory Markers ◽  
Inflammatory Marker ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Tnf Α

Background: A possible relationship between thyroid hormones and glucose metabolism in diabetes has already been established. Objectives: We aimed to evaluate the thyroid function markers and their relationship with inflammation, which is considered as a pathogenic condition of diabetes. Methods: This cross-sectional study included 276 patients with type 2 diabetes. Serum levels of thyroid (TSH, FT4, and FT3) and inflammatory markers (CRP, IL-6, and TNF-α) were measured. Results: The mean age of the subjects was 55.2 years and mean diabetes duration of 16.8 years. The inflammatory markers showed significant differences with the tertiles of TSH and thyroid hormones. TSH was significantly correlated with inflammatory markers, IL-6 (r = 0.13, P = 0.020) and TNF-α (r = 0.17, P = 0.003), while FT4 had a correlation only with TNF-α (r = 0.25, P = <0.001). FT3 was negatively correlated with inflammatory marker IL-6 (r = -0.14, P = 0.020), HbA1c (r = -0.12, P = 0 .040), and HOMA-IR (r = -0.17, P = 0.010). Conclusion: Abnormalities in the thyroid hormone metabolism are related to the increased inflammatory activity as well as insulin resistance, and are associated with the disorders of glucose metabolism.

scholarly journals Circulating Levels of Soluble Receptor Activator of NF-κB Ligand and Matrix Metalloproteinase 3 (and Their Antagonists) in Asian Indian Patients with Ankylosing Spondylitis

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Varun Dhir ◽  
Rajni Srivastava ◽  
Amita Aggarwal
Keyword(s):  
Ankylosing Spondylitis ◽  
Asian Indian ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Matrix Metalloproteinase 3 ◽  
Indian Patients ◽  
Receptor Activator ◽  
And Gender ◽  
Circulating Levels

Background. Bone loss in ankylosing spondylitis may be related to inflammation. Data from previous studies on circulating levels of sRANKL, OPG, MMP3, and TIMP is inconsistent; thus this study is planned to look at this aspect in Asian Indian patients.Methods. Cross-sectional study included patients with ankylosing spondylitis and age- and gender-similar controls. Serum levels of sRANKL, OPG, MMP-3, and TIMP-1 were measured by ELISA.Results. Included 85 patients (M : F = 82 : 3) having mean age (±SD)33.0±10.0years and disease duration11.3±7.3years. BASDAI, BASFI, BASMI, and ESR were4.0±2.2,3.9±2.8,3.0±2.8,and59.2±31.2,respectively. Patients had higher mean (±SD) OPG level (649.7±286.8,389.3±244.8 pg/mL,P<0.001). However, there was no difference in sRANKL (349.2±872.0,554.7±1850.1,P=ns). Serum MMP-3 (91.4±84.7,55.9±37.1 ng/mL,P<0.01) and TIMP-1 (520.6±450.7,296.5±114.2 ng/mL,P<0.001) levels were higher in patients; however, there was no difference in MMP-3/TIMP-1 ratio.Conclusion. Circulating levels of OPG were higher; however, there was no difference in sRANKL in Asian Indian ankylosing spondylitis patients. Although both MMP-3 and TIMP-1 were raised, their ratio was not different from that of controls.

Tamoxifen, Soy, and Lifestyle Factors in Asian American Women With Breast Cancer

2007 ◽  
Vol 25 (21) ◽  
pp. 3024-3030 ◽  
Author(s):  
Anna H. Wu ◽  
Malcolm C. Pike ◽  
Lee D. Williams ◽  
Darcy Spicer ◽  
Chiu-Chen Tseng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Asian American ◽  
Menopausal Status ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Health Concern ◽  
Blood Levels ◽  
Cross Sectional ◽  
Circulating Levels

PurposeSoy foods have been a staple in Asia for centuries but the consumption of this food in the West is recent. Intake of soy among women at high risk for or with breast cancer has become a public health concern because genistein, a major component of soy, has weak estrogenic effects on breast epithelium, and has been found to negate the benefit of tamoxifen in some animal and in vitro studies.Patients and MethodsWe conducted a cross-sectional study in Asian Americans with breast cancer who were tamoxifen users (n = 380) to investigate the association between soy intake and circulating levels of tamoxifen and its metabolites (N-desmethyl tamoxifen [N-DMT], 4-hydroxytamoxifen [4-OHT], and 4-hydroxy-N-desmethyl-tamoxifen [endoxifen]).ResultsSerum levels of tamoxifen or its metabolites were unrelated to self-reported intake of soy or serum levels of isoflavones. Blood levels of tamoxifen were 81% higher in postmenopausal women age 65 or older compared with premenopaual women age 45 or younger (P = .005); similar patterns of results were observed for the tamoxifen metabolites. Levels of N-DMT were 27% (P = .03) lower among women in the highest tertile of body mass index (BMI, > 24.4 kg/m2) compared with those in the lowest category (BMI ≤ 21.5). Women who used hypertensive medications had higher levels of tamoxifen (P = .02) and N-DMT (P = .04) compared with nonusers.ConclusionWe found no evidence that soy intake adversely affected levels of tamoxifen or its metabolites. However, age, menopausal status, BMI, and use of hypertensive medications significantly influenced circulating levels of tamoxifen and its metabolites in this population.

scholarly journals The effect of gamma radiation on plasma levels of zinc and selenium in nuclear medicine staff

2018 ◽  
Vol 21 (1) ◽  
pp. 51-56
Author(s):  
Mahdiyeh Mirnam Niha ◽  
Mehdi Salehi Barough
Keyword(s):  
Nuclear Medicine ◽  
Gamma Radiation ◽  
Graphite Furnace ◽  
Serum Zinc ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Mean

Background and aims: Gamma radiation is widely being used in medical-imaging centers. The aim of this study was to measure possible alterations in serum zinc and selenium levels among nuclear medicine staff. Methods: This cross-sectional study was carried out on 30 nuclear medicine staff as well as on 10 individuals as the control group. Blood samples (20 mL) were obtained from the participants and the serum specimens were isolated by centrifugation at 4000 rpm for 7 minutes. The samples were then used for measurement of zinc and selenium levels. Zinc and selenium levels were measured using atomic absorption spectroscopy (AAS) with a graphite furnace. The mean serum levels of zinc and selenium were compared using SPSS. Results: The mean level of zinc in the radiated group (70.91±14.46 μmol/L) was significantly lower than that in the control group (89.75±17.35 μmol/L) (P=0.002). A negative significant correlation was found between the duration of exposure to radiation and zinc levels (P=0.005). Furthermore, a negative significant relationship was observed between the mean radiation dose after 5 years and zinc concentration (P=0.019). Non-significant difference was found in the mean level of selenium between control (1.71±0.35 μmol/L) and radiation (2.13±1.12 μmol/L) groups. Furthermore, no significant correlation was found between selenium level and exposure time. Conclusion: Based on the results, declined level of zinc can be considered as one of the possible mechanisms caused by gamma radiation on cells which may be associated with oxidative damage. Therefore, zinc therapy can be helpful for those who work at medical radiation centers; however, it merits further studies.

scholarly journals HMGB1, NLRP3, IL-6 and ACE2 levels are elevated in COVID-19 with headache: a window to the infection-related headache mechanism

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hayrunnisa Bolay ◽  
Ömer Karadas ◽  
Bilgin Oztürk ◽  
Riza Sonkaya ◽  
Bahar Tasdelen ◽  
...  
Keyword(s):  
Serum Levels ◽  
Pulmonary Involvement ◽  
Cross Sectional Study ◽  
Trigeminal System ◽  
Cross Sectional ◽  
Trigeminal Neurons ◽  
Moderate Disease ◽  
The Mean ◽  
Inflammatory Molecules ◽  
Second Wave

Abstract Background and aim Pathogenesis of COVID-19 -related headache is unknown, though the induction of the trigeminal neurons through inflammation is proposed. We aimed to investigate key systemic circulating inflammatory molecules and their clinical relations in COVID-19 patients with headache. Methods This cross-sectional study enrolled 88 COVID-19 patients, hospitalized on a regular ward during the second wave of the pandemic. Clinical characteristics of COVID-19 patients were recorded, and laboratory tests were studied. Results The mean ages of 48 COVID-19 patients with headache (47.71 ± 10.8) and 40 COVID-19 patients without headache (45.70 ± 12.72) were comparable. COVID-19 patients suffered from headache had significantly higher serum levels of HMGB1, NLRP3, ACE2, and IL-6 than COVID-19 patients without headache, whereas CGRP and IL-10 levels were similar in the groups. Angiotensin II level was significantly decreased in the headache group. COVID-19 patients with headache showed an increased frequency of pulmonary involvement and increased D- dimer levels. Furthermore, COVID-19 was more frequently associated with weight loss, nausea, and diarrhea in patients with headache. Serum NLRP3 levels were correlated with headache duration and hospital stay, while headache response to paracetamol was negatively correlated with HMGB1 and positively associated with IL-10 levels. Conclusion Stronger inflammatory response is associated with headache in hospitalized COVID-19 patients with moderate disease severity. Increased levels of the circulating inflammatory and/or nociceptive molecules like HMGB1, NLRP3, and IL-6 may play a role in the potential induction of the trigeminal system and manifestation of headache secondary to SARS-CoV-2 infection.

scholarly journals Increased Serum Levels of IFN-γ, IL-1β, and IL-6 in Patients with Alopecia Areata and Nonsegmental Vitiligo

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Katarzyna Tomaszewska ◽  
Magdalena Kozłowska ◽  
Andrzej Kaszuba ◽  
Aleksandra Lesiak ◽  
Joanna Narbutt ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alopecia Areata ◽  
Skin Diseases ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Cytokine Levels ◽  
Common Skin ◽  
Registration Number ◽  
Ifn Γ

Alopecia areata (AA) and vitiligo are both common skin diseases of autoimmune origin. Both alopecia areata and vitiligo have shown to be affected by oxidative stress. The present work is aimed at evaluating and comparing the serum proinflammatory cytokine levels in AA and nonsegmental vitiligo (NSV). A cross-sectional study was conducted of 33 patients with AA, 30 patients with NSV, and 30 healthy controls. Serum levels of interferon γ (IFN-γ), interleukin- (IL-) 1β, and IL-6 were determined quantitatively by ELISA method. Our analysis identified a signature of oxidative stress associated with AA and NSV, characterized by elevated levels of IFN-γ (AA: p=0.007283; NSV: p=0.038467), IL-1β (AA; NSV: p≤0.001), and IL-6 (AA; NSV: p≤0.001). IL-6 was also significantly increased in NSV patients in comparison with AA patients (p=0.004485). Our results supported the hypothesis that oxidative stress may play a significant role in promoting and amplifying the inflammatory process both in AA and vitiligo. The complex understanding of both disease etiopathogenesis involves interrelationships between oxidative stress and autoimmunity. The clinical study registration number is RNN/266/16/KE.

scholarly journals Anion Gap as a Determinant of Ionized Fraction of Divalent Cations in Hemodialysis Patients

2017 ◽  
Vol 13 (2) ◽  
pp. 274-281 ◽  
Author(s):  
Yusuke Sakaguchi ◽  
Takayuki Hamano ◽  
Keiichi Kubota ◽  
Tatsufumi Oka ◽  
Satoshi Yamaguchi ◽  
...  
Keyword(s):  
Divalent Cations ◽  
Cross Sectional Study ◽  
Anion Gap ◽  
Maintenance Hemodialysis ◽  
Sectional Study ◽  
Cross Sectional ◽  
Ionized Magnesium ◽  
The Mean ◽  
Circulating Levels ◽  
Total Magnesium

Background and objectivesCirculating levels of anions that bind to magnesium and calcium are often altered in patients with CKD. However, it is unknown how these alterations affect the ionized fraction of magnesium and calcium.Design, setting, participants, & measurementsThis cross-sectional study involved patients on maintenance hemodialysis and patients not on dialysis who visited the outpatient department of nephrology. We collected whole-blood samples to measure ionized magnesium and calcium concentrations. Adjusted anion gap was calculated as an integrative index of unmeasured anions.ResultsA total of 118 patients on hemodialysis and 112 patients not on dialysis were included. Although the prevalence of hypermagnesemia defined by total magnesium was much higher in patients on hemodialysis than in patients not on dialysis (69% versus 12%; P<0.001), the prevalence of hypermagnesemia defined by ionized magnesium did not differ significantly (13% versus 18%; P=0.28). Among patients on hemodialysis with high total magnesium, 83% had normal or low ionized magnesium. Consequently, the mean ionized fraction of magnesium in patients on hemodialysis was significantly lower than that in patients not on dialysis (51% versus 63%; P<0.001). Similarly, the mean ionized fraction of calcium in patients on hemodialysis was lower than that in patients not on dialysis (55% versus 56%; P<0.001). In patients on hemodialysis who had a higher adjusted anion gap than patients not on dialysis (mean [SD]: 14.1 [2.2] versus 5.1 [3.1]), the ionized fractions of magnesium and calcium were inversely associated with the adjusted anion gap. Furthermore, the anion gap significantly improved predictions of ionized magnesium and calcium in patients on hemodialysis.ConclusionsAnions that accumulate in patients on hemodialysis contribute to the lower ionized fraction of magnesium and calcium. Equations that incorporate the anion gap provide better predictions of ionized magnesium and calcium in patients on hemodialysis.

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