scholarly journals Nephroprotective Effect of Cissampelos owariensis Extract on Renal Histomorphology of Wistar Rats during Exposure to Carbon Tetrachloride- induced Nephropathy

2020 ◽  
pp. 1-10
Author(s):  
Dayo Rotimi Omotoso ◽  
Olayinka Simbiat Lawal ◽  
Oluwasegun Davies Olatomide ◽  
Itohan Grace Okojie
Keyword(s):  
Phenolic Compounds ◽  
Treatment Group ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Kidney Tissue ◽  
Antioxidant Property ◽  
The Body ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Histopathological Changes

To assess nephroprotective potency of methanolic extract of C. owariensis on renal histomorphology of Wistar rats during exposure to nephropathic activity of CCl4. Twenty eight (28) albino Wistar rats divided into four groups which include normal control group administered with vehicles -distilled water (1 ml/kg b.w.) and olive oil (3 ml/kg b.w.), experimental control group administered with CCl4 (3 ml/kg b.w.) twice a week, first treatment group administered with CCl4 (3 ml/kg b.w.) twice a week + methanolic extract of C. owariensis (100 mg/kg b.w.) daily and second treatment group administered with CCl4 (3 ml/kg b.w.) twice a week + methanolic extract of C. owariensis (300 mg/kg b.w.) daily for twenty eight (28) days. Phytochemical analysis of methanolic extract of C. owariensis was carried out using GC-MS. The body weight of study animals was measure at days 0, 7, 14, 21 and 28 of study. Then, kidney tissue of study animals was collected, weighed and processed for histopathological study. Tissue sections were stained using H & E, examined under microscope, photomicrographs were generated and observable histopathological changes were quantified using image-J software. Phytochemical analysis of methanolic extract of C. owariensis showed abundance of phenolic compounds which may in turn confer antioxidant property on the extract. Results of this study also showed that treatment with extract helped to reduce body and tissue weight loss that follows exposure to CCl4. Also, treatment with the extract helped to reduce significantly (p < 0.05) renal histopathological changes following exposure to CCl4. The methanolic extract of C. owariensis contains abundant phenolic compounds which confer antioxidant property that in turn mediate the nephroprotective potency of the extract against nephropathic effect of CCl4.

scholarly journals GAMBARAN HISTOPATOLOGI HATI TIKUS WISTAR YANG DIBERIKAN JUS TOMAT (Solanum Lycopersicum) PASCA KERUSAKAN HATI WISTAR YANG DIINDUKSI KARBON TETRAKLORIDA (CCl4)

2013 ◽  
Vol 1 (3) ◽  
Author(s):  
Eka Sari Tappi ◽  
Poppy Lintong ◽  
Lily Loho
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Liver Cells ◽  
The Body ◽  
Tomato Juice ◽  
Control Group ◽  
Histopathological Changes ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Abstract: Liver is the largest organ in the abdominal cavity. As the center of metabolism in the body, liver is potentially damaged by exposure of toxic substances, inter alia carbon tetrachloride (CCl4). Metabolism of carbon tetrachloride (CCl4) produces CCl3 free radicals that can damage the liver. In Indonesia, there are a lot of natural ingredients that have antioxidant properties, such as tomato. Lycopene in tomatoes contains antioxidant compounds that can prevent damages due to free radical. This study aimed to obtain liver histopathological changes of wistar rats fed with tomato juice after being induced of carbon tetrachloride (CCl4). This was an experimental study, using 10 wistar rats which were divided into 4 groups. Group I was the negative control; group II was induced with CCl4 0,05 cc/day and was terminated on day 6; group III was induced with CCl4 0,05 cc/day and was given tomato juice 3 ml/day, and terminated on day 13; group IV was induced by CCl4 0,05 cc/day, given regular pellets, and terminated on day 13. The results showed that group II had histopathological changes of the liver indicating fatty liver, meanwhile group III showed regeneration of nearly all liver cells. Conclusion: Administration of tomato juice after the induction of 3 ml carbon tetrachloride (CCl4) for 7 day showed regeneration of almost all liver cells. Keywords: histopathological changes of the liver, carbon tetrachloride, tomato juice.   Abstrak: Hati merupakan organ terbesar dalam rongga abdomen, dan pusat metabolisme tubuh dengan fungsi yang sangat kompleks dan sangat berpotensi mengalami kerusakan akibat terpapar oleh bahan-bahan toksik, salah satunya yaitu karbon tertraklorida (CCL4). Metabolisme CCl4 menghasilkan radikal bebas CCl3 yang dapat merusak hati. Di Indonesia terdapat  banyak sekali bahan-bahan alami yang mempunyai kandungan antioksidan, salah satunya yaitu tomat. Tomat mengandung senyawa likopen sebagai antioksidan yang dapat mencegah kerusakan jaringan akibat radikal bebas. Penelitian ini bertujuan untuk mendapatkan gambaran histopatologi hati tikus wistar yang diberi jus tomat pasca induksi karbon tetraklorida (CCl4). Metode penelitian ialah eksperimental. Sampel sebanyak 10 ekor tikus wistar yang dibagi dalam 4 kelompok. Kelompok I sebagai kontrol negatif; kelompok II diinduksi CCl4 0,05cc/perhari dan diterminasi hari ke-6; kelompok III diinduksi CCl4 0,05 cc/hari kemudian diberikan jus tomat 3ml/hari;  dan kelompok IV diinduksi CCl4 0,05 cc/hari kemudian diberikan pelet biasa dan diterminasi hari ke-13. Hasil penelitian menunjukkan pada pemberian CCl4 pada tikus wistar selama 5 hari terdapat gambaran morfologik perlemakan sel hati. Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) menunjukkan terjadinya regenerasi pada hampir seluruh sel-sel hati. Simpulan: Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) selama 7 hari menunjukkan regenerasi pada hampir seluruh sel-sel hati. Kata kunci: gambaran histopatologi hati, karbon tetraklorida, jus tomat.

scholarly journals STUDY OF Coleus amboinicus STEM EXTRACT IN INHIBITING MACROPHAGE CD-68 EXPRESSION IN WISTAR RATS WITH URIC ACID-INDUCED NEPHROPATHY

2019 ◽  
Vol 13 (3) ◽  
Author(s):  
Rondius Solfaine ◽  
Wida Wahidah Mubarokah ◽  
Lailatul Muniroh
Keyword(s):  
Uric Acid ◽  
Treatment Group ◽  
Wistar Rats ◽  
Kidney Tissue ◽  
Control Group ◽  
Renal Tubules ◽  
Cd 68 ◽  
Stem Extract ◽  
Coleus Amboinicus ◽  
Oxonic Acid

The purpose of this study was to evaluate effect of Coleus amboinicus (CA) stem extracts on uric acid-induced nephropathy by comparingthe levels of Macrophage CD-68 expression and concentration of serum Cystatine C (CYS C ) in Wistar rats. Twenty-four male Wistar rats(Rattus norvegicus) with a body weight (bw) of 200-250 g, were allocated into three groups, with eight animals per group. The rats in controlgroup (PO) received 0.1% carboxymethyl cellulose (CMC) solution orally The rats in group 2 (P1) were orally induced with uric acid (UA) (500mg/kg) and oxonic acid (OA) (750 mg/kg.) and the rats in group 3 (P2) received uric acid (500 mg/kg), oxonic acid (750 mg/kg), and 500 mg/kgof the CA stem extracts for 35 days. Bloods were collected for analysis of serum CYS C expression and concentration of serum creatinine andblood nitrogen urea (BUN). The rats in all groups were sacrificed for kidney tissue extractions for macrophage CD-68 identification andhistopathology analysis. The levels of CYS C concentrations were analyzed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. Theresults showed that Coleus amboinicus stem extract at dose of 500 mg/kg bw can significantly reduce BUN and creatinine levels (P≤0.05), whileCys C levels were not different. In the treatment group (P2) compared with group (P1). CD-68 (ED-1) macrophage activity decreasedsignificantly (P≤0.05) in the treatment group (P2) compared to the control group and (P1). Nephrophaty induction using UA and OA causessevere kidney lesions characterized by degeneration, necrosis and inflammation of the renal tubules and glomerulus in the treatment group.

scholarly journals Histopathological Changes in Gastrointestinal Tissues of Wistar Rats Administered with Methanolic Leaf Extract of Caladium bicolor (Araceae)

2020 ◽  
pp. 12-19
Author(s):  
Dayo Rotimi Omotoso ◽  
Adeniran Oluwadamilare Akinola ◽  
Ibifuro Brown
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Body Weight Loss ◽  
The Body ◽  
Control Group ◽  
Surface Erosion ◽  
Histopathological Changes ◽  
Experimental Animals ◽  
Toxic Potential

To assess the effect of methanolic leaf extract of Caladium bicolor on the histomorphology of gastrointestinal tissues of experimental animals. Twenty four Wistar rats (weighing between 175-190 g) were randomly and equally divided into four groups which include one control group (CG) and three treatment groups (TG I, TG II and TG III). The CG was administered with distilled water [2 ml/kg body weight (b.w.)] while TGs I, II and III were administered with 100 ml/kg, 200 ml/kg and 300 ml/kg (b.w.) of C. bicolor extract respectively. All administrations were done orally and once daily for a period of thirty days. The body weight of all animals was recorded at the beginning and end of study. After the period of study, gastric and small intestinal tissues of experimental animals were harvested, processed, converted to tissue blocks and sectioned. Tissue sections were stained using Haematoxylin and Eosin (H&E) technique. Thereafter, stained sections microscopically examined for observable histopathological changes within study tissues. The results of this study showed that exposure to C. bicolor extract causes significant (p < 0.05) body weight loss in TGs I-III compared to CG. In addition, prominent histopathological changes were observed in gastrointestinal tissues of experimental animals in TGs I-III including gastric mucosal surface erosion and intestinal villi degeneration compared to normal gastrointestinal histomorphology of CG animals. These histopathological changes may be associated with toxic effect of phytochemicals constituents of the extract. Therefore, its application for therapeutic purposes needs to be thoroughly re-validated or perhaps disallowed where alternative therapeutic agents with minimal toxic potential exist.

scholarly journals Assessment of histo-reparative effect of methanolic extract of Camellia sinensis in rat model of Lead-induced testicular damage

2020 ◽  
Vol 10 (4-s) ◽  
pp. 46-52
Author(s):  
Dayo Rotimi Omotoso ◽  
Adeniran Oluwadamilare Akinola ◽  
Winners Prince Ehiemere
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Camellia Sinensis ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Control Group ◽  
Testicular Damage ◽  
Male Wistar Rats ◽  
Reparative Effect

Objective: To assess reparative effect of methanolic extract of Camellia sinensis on testicular histomorphology of male Wistar rats exposed to lead-induced testicular damage.  Methods: Twenty four male Wistar rats were grouped into four A–D (n = 6). These include normal control group A (distilled water 5 ml/kg), test control group B (lead acetate 30 mg/kg), treatment group C (lead acetate 30 mg/kg + MECS 5 mg/kg) and treatment group D (lead acetate 30 mg/kg + MECS 10 mg/kg). Administrations were through oral route for a period of 60 days and body weight of study animals was recorded during days 0, 20, 40 and 60 of study. Phytochemical analysis of extract was done using GCMS. At the end of sudy, testicular tissue weight of study animals was measured followed by histopathological study. Results: The extract significantly ameliorated body weight loss and exhibited histo-reparative effect on testicular histomorphology of study animals following lead exposure. Conclusion: Methanolic extract of Camellia sinensis exhibits reparative effect against toxic effect of tissue toxicant due to its antioxidant activity. Keywords: Camellia sinensis, Lead toxicity, Testis histomorphology, Wistar rats

scholarly journals The increase of VEGF expressions and new blood vessels formation in Wistar rats induced with post-tooth extraction sponge amnion

2018 ◽  
Vol 51 (1) ◽  
pp. 1
Author(s):  
Moh. Basroni Rizal ◽  
Elly Munadziroh ◽  
Indah Listiana Kriswandini
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Blood Vessels ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Freeze Dried ◽  
Significant Difference

Background: Tooth extraction is the process of removing a tooth from the oral cavity potentially triggering a wound healing response in the body. As a result, many methods have been applied to improve the wound healing process, especially in wounds resulting in complications. One such method involves the application of amniotic membrane which has anti-inflammatory, anti-bacterial, anti-fibrosis, anti-scarring properties with low immunogenicity, epithelialization effects, and secretory leukocyte protease inhibitor (SLPI). It also contains collagen, various growth factors, transferrin, fibronectin, nidogen, proteoglycans, hyaluronan, and laminin. Purpose: This study aimed to determine the effects of sponge amnion on the number of VEGF expressions and new blood vessels in post-tooth extraction wounds of Wistar rats. Methods: Sponge amnion was produced by mixing freeze-dried amnion membrane from the Tissue Bank at RSUD Dr. Soetomo with 1% gelatin before freeze drying the mixture. Wistar rats were then divided into two groups. In Group 1, referred to as the control group, the post-extraction wounds of the rats received no treatment. Meanwhile, in Group 2, the treatment group, the subjects’ post-extraction wounds were treated with sponge amnion. The rats of both groups were sacrificed on day 3 to allow observation of the number of VEGF expressions and new blood vessels. A statistical analysis test, a t-test, was subsequently conducted. Results: There was a significant difference in the number of new blood vessels in the control group and that of the treatment group with a p value of 0.018 (p<0.05). There was also a significant difference in VEGF expression between the two groups with a p value of 0.000 (p <0.05). Conclusion: Sponge amnion can generate a number of VEGF expressions and new blood vessels in the post-extraction wounds of Wistar rats.

Histomorphometric Alterations in Hepatic Tissue from Malathion-Induced Toxicity: An Experimental Animal Study

2021 ◽  
Vol 10 (1) ◽  
pp. 370-375
Author(s):  
Shazia Parveen Channar ◽  
Nasreen Qazi ◽  
Sajjad Ali Almani ◽  
Sehar Gul Memon ◽  
Mansoor Mukhtar Qazi ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Low Dose ◽  
The Body ◽  
Hepatic Tissue ◽  
Control Group ◽  
High Dose ◽  
Dependent Manner ◽  
Histopathological Changes ◽  
Group A ◽  
Group B

Background: Malathion, a widely used insecticide readily absorbed through skin and seriously affects different tissues and organs of the body. The main objective of this study was to compare the histomorphometric alterations resulting from hazardous effects of different doses of Malathion on hepatic tissue of male albino Wistar rats. Material and Methods: This animal experimental study was conducted at the Department of Anatomy and Postgraduate Research Laboratory at the Isra University, Hyderabad, Sindh Pakistan from February to July 2019. Thirty male albino Wistar rats between 250-300 grams weight were distributed equally into group A (control), group B (low-dose Malathion group; 27mg/kg 1/50 of LD50), and group C (high-dose Malathion group; 50mg/kg). Bodyweight of all rats was taken twice, before and after the experiment. The liver was dissected out, washed and weighed. Histopathological examination was done under the light microscope. Grading was done for severity in histopathological changes in each group. Data was analyzed using one-way ANOVA and Post-hoc Tukey test for comparison with the level of significance set at P-value ≤ .05. Results: Statistically significant (P < .05) decline in body weight was observed in groups B and C in comparison with group A. The relative weight of the liver was increased significantly (P < .05) in the experimental groups, when compared with the control group. Mild-to-moderate histopathological changes were observed in the low-dose Malathion group (Group B) while moderate-to-severe histopathological changes were demonstrated in the high-dose group (Group C). Conclusions: Malathion is a potent toxic pesticide and its exposure can exhibit damage to the hepatic tissues in a dose-dependent manner.

Perbandingan Efek Suplementasi Tablet Tambah Darah Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Pada Ibu Hamil Dengan Usiakehamilan 16-32 Minggu Di Desa Keniten Kecamatan Mojo Kabupaten Kediri

2017 ◽  
Vol 3 (1) ◽  
pp. 76
Author(s):  
Siti Asiyah ◽  
Dwi Estuning Rahayu ◽  
Wiranti Dwi Novita Isnaeni
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Gestational Age ◽  
Group Treatment ◽  
Sampling Technique ◽  
Hemoglobin Level ◽  
The Body ◽  
Control Group ◽  
Fetal Organ ◽  
And Control

The needed of Iron Tablet in pregnancy was increase than mother who not pregnant.  That  cause of  high metabolism at the pregnancy for formed of  fetal organ and energy. One of effort for prevent anemia in mother pregnant with giving the Iron tablet and vitamin c. The reason of  this research in 4 June – 11 July 2014 is for compare the effect of  iron tablet suplementation with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks In Desa Keniten Kecamatan Mojo Kabupaten Kediri. This research method using comparative analytical.  Research design type of Quasy Eksperiment that have treatment group and control group. Treatment group will giving by Iron tablet and 100 mg vitamin C, and control group just giving by iron tablet during 21 days. Population in this research are all of mother pregnant with Gestational Age Of 16-32 Weeks with Sampling technique is  cluster random sampling is 29 mother pregnant. Comparison analysis of  iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks, data analysis using Mann Whitney U-test and the calculated U value (44,5) less than U-table (51). So there was difference of iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks Therefore, the addition of vitamin C on iron intake is needed to increase the uptake of iron tablets. When the amount of iron uptake increases, the reserves of iron in the body will also increase, so as to prevent anemia in pregnant women; Keywords : Iron Tablet (Fe), Vitamin C, Hemoglobin level, Mother Pregnant

Effect of High Plane of Nutrition on Blood Biochemical Profile and Onset of Puberty in Prepubertal HF X Kankrej Crossbred Heifers

2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Plasma Levels ◽  
Age Of Onset ◽  
The Body ◽  
Monthly Interval ◽  
Control Group ◽  
Blood Biochemical ◽  
Lower Protein ◽  
The Mean

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.

scholarly journals Impact of Vanadium Complexes Treatment on the Oxidative Stress Factors in Wistar Rats Plasma

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
R. Francik ◽  
M. Krośniak ◽  
M. Barlik ◽  
A. Kudła ◽  
R. Gryboś ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
The Body ◽  
Stress Factors ◽  
Control Group ◽  
Vanadium Complexes ◽  
Healthy Male ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters

The aim of this study was to investigate the clinical efficacy of vanadium complexes on triglycerides (TG), total cholesterol (Chol), uric acid (UA), urea (U), and antioxidant parameters: nonenzymatic (FRAP—ferric reducing ability of plasma, and reduced glutathione—GSH) and enzymatic (glutathione peroxidase—GPx, catalase—CAT, and GPx/CAT ratio) activity in the plasma of healthy male Wistar rats. Three vanadium complexes: [VO(bpy)2]SO4⋅2H2O, [VO(4,4′Me2bpy)2]SO4⋅2H2O, and Na[VO(O2)2(bpy)]⋅8H2O are administered by gavage during 5 weeks in two different diets such as control (C) and high fatty (F) diets. Changes of biochemical and antioxidants parameters are measured in plasma. All three vanadium complexes statistically decrease the body mass growth in comparison to the control and fatty diet. In plasma GSH was statistically increased in all vanadium complexes-treated rats from control and fatty group in comparison to only control group. Calculated GPX/CAT ratio was the highest in the control group in comparison to others.

Effect Of Pregnancy Exercise On Third Trimester Primigravida Anxiety In Dealing With Childbirth

2021 ◽  
pp. 76-83
Author(s):  
Ismi Puji Astuti
Keyword(s):  
Treatment Group ◽  
Sampling Technique ◽  
The Body ◽  
Rank Test ◽  
Control Group ◽  
P Value ◽  
Research Subjects ◽  
Third Trimester ◽  
Quasi Experimental ◽  
Signed Rank Test

Background: The first labor anxiety is an unpleasant psychological condition due to physiological changes that cause psychological instability. The condition of excessive anxiety, worry, fear without cause, and stress, causes the muscles of the body to tense up, especially the muscles in the birth path to become stiff and hard, making it difficult to expand. This study analyzed the effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth. Methods: Quasi experimental design with nonrandomized pretest-postest control group. The sampling technique used was purposive sampling with 34 research subjects. 17 people for the treatment group were given pregnancy exercise 8 times, and 17 people for the control group were given health education about preparation for childbirth. Results: The results of data analysis using the Wilcoxon signed rank test showed that the average reduction in the treatment group was 8.00 and the control group was 3.00. The effect of pregnancy exercise on third trimester primigravida anxiety in the treatment group with a p-value (0.000) <(0.05), while the control group with a p-value (0.180)> (0.05). Conclusion: There is an effect of pregnancy exercise on third trimester primigravida anxiety in dealing with childbirth in the Wuryantoro Health Center work area. 

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