scholarly journals Phytochemical Screening and Determination of Polyphenols in the Hydro-Ethanolic Extract of Trunk Bark and Its Fractions of Stereospermum kunthianium Cham (Bignoniaceae)

2021 ◽  
pp. 1-9
Author(s):  
Abdou Sarr ◽  
Serigne Ibra Mbacké Dieng ◽  
Kady Diatta-Badji ◽  
Amadou Ibrahima Mbaye ◽  
William Diatta ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Aluminium Chloride ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Healing Agent

Background : Stereospermum kunthianum Cham., is a medicinal plant from the Bignoniaceae family, used in Ferlo (Senegal) against stomach aches, as a healing agent and aphrodisiac.Aims/Objective : The aim of this study was to contribute to the valorisation of the plant by carrying out a phytochemical screening followed by an assay of the polyphenols of the hydro-ethanolic extract of the bark and its fractions.Methods : Phytochemical screening was carried out by coloring and/or precipitation reactions. The total polyphenol and tannin contents were evaluated by the Folin-Denis method and the flavonoid content by a method using aluminium chloride (AlCl 3 ) and sodium nitrite (NaNO 2 ). Results: The search for chemical compounds revealed the presence of various secondary metabolites such as gallic and catechic tannins, flavonoids, saponosides, alkaloids, sterols and polyterpenes. The total polyphenol contents of the samples ranged from 0.81 ± 0.02 to 13.52 ± 0.06 mg EAT / g. Those of tannins and flavonoids were between 0.03 ± 0.01 and 4.56 ± 0.02 mg EAT / g and between 1.13 ± 0.04 to 31.88 ± 0.19 mg ER / g respectively.Conclusion : These different metabolites would be responsible for the activities noticed for this plant.

scholarly journals New alkaloids from Ocotea duckei vattimo (Lauraceae)

2021 ◽  
Vol 10 (4) ◽  
pp. e28910412800
Author(s):  
Laisla Rangel Peixoto ◽  
Josean Fechine Tavares ◽  
Yuri Mangueira do Nascimento ◽  
Augusto Lopes Souto ◽  
Raimundo Braz Filho ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Spectroscopic Methods ◽  
Drying Process ◽  
Isolation And Identification ◽  
Northeast Region ◽  
Toxicological Studies ◽  
Alkaloid Fraction

Ocotea duckei Vattimo, popularly known as “louro-de-cheiro”, “louro-pimenta”, and “louro-canela”  is a member of the Lauraceae family found in the Northeast region of Brazil. It is popularly used to treat neuralgia, dyspepsia, anorexia and pain. This current study aimed to promote the isolation and the identification / determination of new secondary metabolites of the species O. duckei Vattimo. In order to obtain the vegetable drug of O. duckei, its stem barks were harvested and submitted to a drying process followed by pulverization. The Crude Ethanolic Extract (BSE), obtained from the vegetable drug, was then subjected to an alkaloid extraction protocol in order to generate its Total Alkaloid Fraction (TAF). The isolation and identification of chemical compounds were carried out by chromatographic and spectroscopic methods respectively. The chemical investigation of O. duckei resulted in the isolation of three compounds: Ocoteaduccin A (OD-1, 0.020 g), Ocoteaduccin B (OD-2, 0.015 g), and N-methylcoclaurine (OD-3, 0.008 g). O. duckei proved to be a promising species, due to the variety of isolated chemical compounds. Further chemical, pharmacological and toxicological studies must be conducted in order to improve the phytochemical knowledge about this plant.

Validasi dari Spektrofotometri UV-Vis dan Kandungan Total Flavonoid Ekstrak Etanol dari Akar Alang-Alang (Imperata cylindrica) dan Daun Pegagan (Centella asiatica) Anita Puspa Widiyana

2021 ◽  
Vol 3 (2) ◽  
pp. 126-136
Author(s):  
Anita Puspa Widiyana ◽  
Keyword(s):  
Chemical Constituents ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Imperata Cylindrica ◽  
Total Flavonoid ◽  
Analysis Method ◽  
Flavonoid Content ◽  
Validation Testing

Validation as a quality control for the content of chemical compounds from natural ingredients. One of the chemical constituents is the flavonoids which are found in the Imperatacylindrica roots and Centella asiatica leaves. This study aims to ensure the analysis method meets the requirements and determines the levels of flavonoids. The research stages included extraction, validation and determination of total flavonoid. Extraction was carried out by immersing dry simplicia in 96% ethanol solvent for 3x24 hours. The solvent is evaporated using a rotary evaporator until a thick extract is formed. Validation testing includes linearity, accuracy, precision, LOD and LOQ. Determinationof the total flavonoid was carried out by measuring the absorption at a maximum wavelength of 428.2 nm. The validation results includethe correlation coefficient (R) of 0.998, precision % RSD <2 %, %accuracy 99,53-97,98%, LOD 3.02ppm and LOQ 9.15 ppm. The total flavonoid of the ethanol extract of Imperata cylindrica roots was 36.39 ± 0.08 mg/g QE and Centella asiatica leaves was 102.10 ± 0.08 mg/g QE. The conclusion is that the method used met the validation requirements and the total flavonoid content of the ethanol extract of Centella asiatica leaves was higher than Imperata cylindrica roots

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals Evaluation of Phytochemical and Antioxidant Activity of Gomphrena celosioides Mart. Grown in Tien Giang Province, Vietnam

2019 ◽  
Vol 32 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Nguyen Van Thuy ◽  
Nguyen Minh Tien ◽  
Nguyen Ngoc Quy ◽  
Tri Duc Lam ◽  
Huynh Thi Kieu Linh ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ether Extract ◽  
Flavonoid Content ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Polyphenol Compounds ◽  
Diethyl Ether Extract ◽  
Gomphrena Celosioides ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds

Gomphrena celosioides Mart. is well known for its medicinal values worldwide. In this study, three extracts, viz. diethyl ether extract (DEE), ethanolic extract (EE) and aqueous extract (AE), were successively obtained from the leaves and stem of Gomphrena celosioides to determine the polyphenol and flavonoid content in this plant. A wide variety of pharmacologically active compounds such as alkaloid, flavonoid, terpenoid, saponin, tannin and polyphenol compounds were present in Gomphrena celosioides. The results of quantitative determination showed that total polyphenol content of DEE, EE and AE reached 35.35 ± 1.47, 250.17 ± 2.95 and 133.92 ± 3.17 mgGAE/g, respectively. Moreover, total flavonoid content of the DEE, EE and AE was 23.21 ± 1.87, 50.74 ± 2.32 and 27.25 ± 1.34 mgQE/g, respectively. In comparison with DEE and AE, the ethanolic extract exhibited the highest DPPH (IC50 = 13.29 ± 0.10 μg/mL) and ABTS (IC50 = 6.3 ± 0.11 μg/mL).

Validation of Spectrophotometric Methods for the Determination of Total Polyphenol and Total Flavonoid Content

2017 ◽  
Vol 100 (6) ◽  
pp. 1795-1803 ◽  
Author(s):  
Petra Matić ◽  
Marija Sabljić ◽  
Lidija Jakobek
Keyword(s):  
Total Flavonoid ◽  
Chlorogenic Acids ◽  
Flavonoid Content ◽  
Polyphenol Content ◽  
Validation Process ◽  
Total Polyphenol ◽  
Spectrophotometric Methods ◽  
Calibration Curves ◽  
Validation Parameters

Abstract The aim of this study was to validate spectrophotometric methods for the measurement of total polyphenol (TP; via the Folin–Ciocalteu method) and total flavonoid (TF) content [via the aluminum chloride (AlCl3) method]. Validation parameters of these methods were determined, including linearity, sensitivity, precision (intra-assay and intermediate), accuracy, LOD, and LOQ. For the validation process, groups of polyphenol standards were used, including phenolic acids (gallic, p-coumaric, caffeic, and chlorogenic acids), flavan-3-ols [(+)-catechin and procyanidins B1 and B2], flavonols (quercetin and quercetin-3-rutinoside), and dihydrochalcones (phloretin and phloretin-2-glucoside). Obtained validation parameters were within acceptable ranges with high determination coefficients, reasonably low LODs and LOQs, and high slopes in the calibration curves for both methods, except for phloretin and phloretin-2-glucoside, for which there were low slopes in the calibration curves for the AlCl3 method. To evaluate differences in polyphenol content, the validated spectrophotometric methods were used to determine TP and TF content in wines (Plavac, Graševina, and Vranac) and juices (blueberry, strawberry, and blackcurrant juice) according to the polyphenol calibration curves. Polyphenol contents were different for both methods in all wines and juices.

scholarly journals A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera

2019 ◽  
Vol 20 (2) ◽  
pp. 17-24
Author(s):  
Hesty Parbuntari ◽  
Sri Benti Etika ◽  
Melindra Mulia ◽  
Elfanny Delvia
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Light Exposure ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
West Sumatra ◽  
Ocimum Tenuiflorum ◽  
Qualitative Test

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.

scholarly journals Phytochemical screening and in vitro anti-inflammatory activity of ethanolic extract of Epidendrum coryophorum leaves.

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1387-1393
Author(s):  
Irina Francesca González Mera ◽  
Orestes Darío López Hernández ◽  
Vivian Morera Córdova
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Inflammatory

Epidendrum coryophorum belongs to the Orchidaceae family. Traditional uses of some species for this genus include infusions of the leaves used for kidney problems, treat influenza, conjunctivitis, liver pain, relieve kidney symptoms, and hypoglycemic effect. This work's objective was to determine the phytochemical profile of the ethanolic extract of Epidendrum coryophorum leaves and to evaluate the potential anti-inflammatory activity in vitro of the extract employing the erythrocyte membrane stabilization method. The phytochemical screening carried out in this work suggested phenols, coumarins, flavonoids, tannins, steroids, and sterols in the ethanolic extract of Epidendrum coryophorum leaves. Cardiotonic glycosides and carbohydrates were also found. The ethanolic extract's UV-Vis spectrum showed absorption maxima at 268 nm and 332 nm, which could correspond to flavonoids of the flavonoid classes, 3-OH substituted flavonols, or isoflavones. The quantitative determination of total phenols of the ethanolic extract was carried out using the Folin-Ciocalteu method. The total phenolic content expressed as mg Gallic acid equivalent (G.A.E.) per gram of extract was found to be 19,96 mgGAE/g of Epidendrum coryophorum. The ethanolic extract of Epidendrum coryophorum leaves showed hemolysis inhibition values ​​of 18,19% at 1,0 mg/mL, 38,98% at 1,5 mg/mL and 40,94% at 2,5 mg/mL compared with aspirin (positive control) giving values ​​of 65,33% at 1,0 mg/mL, 72,26% at 1,5 mg/mL and 73,75% at 2,5 mg/mL. The values ​​obtained for inhibition of hemolysis with ethanolic extract, compared with the values ​​obtained with a pure anti-inflammatory, are significant and demonstrate anti-inflammatory activity in Epidendrum coryophorum. Keywords: Epidendrum coryophorum, total phenolic content, microencapsulation, anti-inflammatory activity

scholarly journals Efektivitas gel ekstrak etanol daun senggani (Melastoma candidum D. Don.) terhadap diameter luka pasca pencabutan gigi pada tikus putih (Rattus norvegicus)

2021 ◽  
Vol 10 (1) ◽  
pp. 44-54
Author(s):  
Sussana Halim ◽  
Hariyanto Halim ◽  
I Nyoman Ehrich Lister ◽  
Saipul Sihotang ◽  
Ali Napiah Nasution ◽  
...  
Keyword(s):  
Wound Healing ◽  
Secondary Metabolites ◽  
Phenolic Compound ◽  
Rattus Norvegicus ◽  
Tooth Extraction ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Average Data

ABSTRAKDaun senggani (Melastoma candidum D. Don.) mengandung flavonoid, triterpenoid, tanin, saponin, steroid, glikosida, dan fenolik yang berfungsi sebagai antioksidan, antiinflamasi, dan bersifat antifungi, antivirus dan antibakteri. Penelitian ini bertujuan untuk mengetahui konsentrasi ekstrak daun senggani (M. candidum D. Don.) yang memberikan efektivitas terbaik terhadap penyembuhan luka pasca pencabutan gigi pada tikus putih (Rattus norvegicus). Hasil ekstraksi daun senggani diformulasikan dalam bentuk salep dengan konsentrasi 1%, 3%, 5%, dan 10%. Hasil data rata-rata persentase kesembuhan luka yang terlihat dari diameter luka diuji secara statistik menggunakan SPSS versi 23. Hasil analisis pada hari ke-5 menunjukkan salep ekstrak etanol daun senggani (M. candidum D. Don.) konsentrasi 10% memiliki efektivitas penyembuhan luka yaitu sekitar 2,96 mm (p>0,05). Hasil skrining fitokimia daun senggani menunjukkan adanya senyawa metabolit sekunder seperti tanin, saponin, flavonoid, streroid/triterpene, dan glikosida. Kata kunci: ekstrak; gel; luka gigi; Melastoma candidum D. Don.  ABSTRACTThe effectiveness of senggani (Melastoma candidum D. Don.) leaves ethanolic gel extract on wound diameter after tooth extraction in Rattus norvegicus.Senggani (Melastoma candidum D. Don.) contains flavonoid, triterpenoid, tanin, saponin, steroid, glicoside, and phenolic compound which use as antioxidant, antiinflamation, antifungus, antivirus, and antibacteria. This study aims to determine the concentration of senggani (M. candidum D. Don.) leaves extract which gives the best effectiveness on wound healing after tooth extraction in Rattus norvegicus. The results of senggani (M. candidum D. Don.) leaves extraction was formulated in the form of ointments with concentrations of 1%, 3%, 5%, and 10%. The results of the average data on the percentage of wound healing visible from the diameter of the wound were statistically tested using SPSS version 23. The results of the analysis on the day 5 showed the ointment of 10% ethanolic extract of senggani (M. candidum D. Don.) leaves  had an effective wound healing of about 2.96 mm (p> 0.05). Furthermore, the results of phytochemical screening are secondary metabolites such as tanins, saponins, flavonoids, streroids/triterpene and glycosides. Keywords: extract; gel; tooth wounds; Melastoma candidum D. Don.

scholarly journals Uji Daya Hambat Ekstrak Etanol Daun Jerangau Hijau terhadap Staphylococcus aureus

2018 ◽  
Vol 1 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Susi Novaryatiin ◽  
Guntur Satrio Pratomo ◽  
Cindia Yunari
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plant ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Extraction Process ◽  
Central Kalimantan ◽  
Percolation Method ◽  
The People ◽  
Anti Inflammatory

Jerangau Hijau is a medicinal plant believed by the people of Central Kalimantan to have an efficacy of treating fever, postpartum injuries, and anti-inflammatory. Based on previous research, Jerangau Hijau is known to contain chemical compounds namely flavonoids and saponins that have activity as an antibacterial. The purpose of this study was to find out whether the ethanolic extract of Jerangau Hijau leaves able to inhibit the growth of Staphylococcus aureus bacteria and to know the concentration of ethanolic extract of Jerangau Hijau leaves that able to inhibit the growth of Staphylococcus aureus bacteria. This research was conducted using the Kirby-Bauer method with disc paper. The extraction process was carried out by percolation method using 96% ethanol solvent. The results showed that the ethanolic extract of Jerangau Hijau leaves was able to inhibit the growth of Staphylococcus aureus bacteria, at concentrations of 1%, 5%, 10%, and 15% with mean inhibitory zones respectively 22�0,2 mm; 32,3�1,4 mm; 26,5�3,8 mm; and 13,1�3 mm.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING, TOTAL PHENOL AND FLAVONOID CONTENT OF MIMOSA RUBICAULIS AND REINWARDITA INDICA

2019 ◽  
pp. 54-58
Author(s):  
ROSHANI GURUNG
Keyword(s):  
Plant Species ◽  
Biological Effects ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Specific Chemical ◽  
Dry Extract

Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica. Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method. Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight followed by stem and root of M. rubicaulis i.e. 381.06±5.23 mg QE/g dry extract weight and 337.43±1.39 mg QE/g dry extract weight. Conclusion: Phytochemical analysis concluded the presence of biologically important phytoconstituents like flavonoid and phenol in both plant species. Further studies, should be carried out to isolate specific chemical constituents and should be used in different studies to explore their biological effects.

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