scholarly journals Effects of Seed Source, Moisture Content and Duration of Storage on the Viability of Vitellaria paradoxa C.F. Gaertn

2019 ◽  
pp. 1-6
Author(s):  
A. F. Aderounmu ◽  
I. O. Asinwa
Keyword(s):  
Storage Temperature ◽  
Seed Viability ◽  
Germination Percentage ◽  
Germination Test ◽  
River Sand ◽  
Temperature Regimes ◽  
Duration Of Storage ◽  
Duration Relationship ◽  
Relationship Of ◽  
Tropical Seeds

Viability of tropical seeds is crucial in plantation establishment. Temperature – moisture duration relationship of the seed is of great significance in seed viability. 3000 ripe fruits of V. paradoxa C. F. Gaertn were collected from each of three sources where it is endemic: Eruwa, Sakiand New-Bussa. The fruits were depulped; a sample of 100 seeds was drawn from each of the three sources and sown in washed and sterilized river sand to monitor the germination percentage of the seeds. The seeds were dried under dehumidifier to attain two moisture contents (43% and 32%). Thereafter, each seed lot from the three sources was divided into two batches (A and B) of 1200 seeds each, divided into four and stored at four different temperature regimes: -20°C, 5°C, 22°C and 28± 2°C. Samples were taken monthly for germination test for six months. Data were subjected to percentages and analysis of variance (ANOVA). Results showed that freshly collected seeds of V. paradoxa had 98% germination when sown within 48 hours of fallen and collection which eventually dropped to 4.4% after the six months storage. Storage temperature had significant effect (P > 0.05) on germination of the seeds. Seeds stored at 28±2°C had the highest mean germination (72.2%), those stored at 22°C had 70.2% while seeds stored at -20°C and 5°C failed to germinate. The seeds of V. paradoxa readily lost viability with time, hence fallen fruits should be collected during fruiting season processed and sown immediately or within 7 days of collection.

Seed Longevity and Germination Behaviour in Eugenia roxburghii

2021 ◽  
Vol 27 (3) ◽  
pp. 167-171
Author(s):  
Shareef Muhammed ◽  
Chitra Rajeswary ◽  
Anil Chandran
Keyword(s):  
Storage Temperature ◽  
Seed Viability ◽  
Seed Longevity ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Germination Behaviour ◽  
Garden Plant ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Eugenia roxburghii is an evergreen graceful shrub with a tremendous potential as garden plant. As a part of ex-situ conservation and popularization of the species, seed longevity was studied by understanding the relationship of seed viability with respect to different moisture contents and storage temperature. Seeds are recognized as recalcitrant, being desiccation as well as chilling sensitive. During hermetic storage, seeds stored at 300C/70%RH retained viability for about 5 months and 4 months in 200C/20% RH. Seeds can be best stored for five months in laboratory conditions.

Seed storage and germination studies of Garcinia talbotii Raizada ex Santapau seeds - an endemic tree from Western Ghats of India

2016 ◽  
Vol 23 (2) ◽  
pp. 73-78
Author(s):  
T. Sabu ◽  
P.S. Shameer ◽  
Chitra Rajeswary ◽  
N. Mohanan ◽  
C. Anilkumar
Keyword(s):  
Ex Situ Conservation ◽  
Storage Temperature ◽  
Seed Viability ◽  
Seed Storage ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Western Ghats Of India ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Seeds of Garcinia talbotii remained viable hardly for two weeks in open room conditions. As part of ex-situ conservation of Garcinia talbotii, seeds longevity was studied since seeds are the main propagule. For this, the relationship of seed viability with respect to different moisture content and storage temperature were analyzed. Seed storage behaviour is also investigated. Being recalcitrant, seeds are desiccation sensitive as well as chilling sensitive. During hermetic storage of seeds at 300C /70 % RH seeds retained viability about 6 months.

scholarly journals SEED VIABILITY TEST OF COMMERSIAL DATES ON THE MARKET

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ekosari Roektiningroem ◽  
Purwanti Widhy
Keyword(s):  
Room Temperature ◽  
Germination Rate ◽  
Seed Viability ◽  
Dry Weight ◽  
Germination Percentage ◽  
State University ◽  
Germination Test ◽  
Viability Test ◽  
Seed Moisture ◽  
Dark Orange

This research aims to to know the seed viability of the two dates on the market. It has been done from July to November 2014, in the laboratory of  Mathematics and Natural Science Faculty, Yogyakarta State University. The type of Dates seeds are Sayer (oval-cilindrical, ±3cm, dark orange brown/more light than another),  and Lulu (round, ±2cm, dark maroon reddish /more dark than Sayer’s). Viability test by germination testing; conducted on plastic tray with cotton sheet and fabric covered; fill with 100 grains of seeds. Repeated 4 times. Incubation at room temperature (±30oC). The parameters were seed fresh weight, seed dry weight, seed moisture, germination percentage and germination rate. The results were analyzed by compare mean - independent samples t test of SPSS 18’th. The results showed that the viability of both of Date’s seeds from local’s market (Sayer and Lulu) is high. Sayer’s have better viability than Lulu’s (due to the germination percentage and germination rate). Keywords: date seed, viability test, germination test

scholarly journals Effects of Desiccation and Storage Temperature on Seed Germination in Kapok

2016 ◽  
Vol 26 (1) ◽  
pp. 83-88
Author(s):  
YanLing Zheng ◽  
GaoJuan Zhao ◽  
HuanCheng Ma
Keyword(s):  
Seed Germination ◽  
Storage Temperature ◽  
Seed Viability ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Deciduous Tree ◽  
Seed Moisture Content ◽  
Seed Collection ◽  
And Storage ◽  
Mature Seeds

Kapok (Bombax ceiba) is a deciduous tree that can grow in the dry-hot valley of southwestern China where its natural regeneration by seedlings is difficult. As mature fruit split open and seeds disperse by wind, it is difficult to collect fully mature seeds. The effects of seed moisture content (MC) and storage temperatures on seed germination of dark-brown seeds collected from split fruit and light-brown seeds collected ≈10–15 days earlier than the time of fruit split were studied to determine the effective germplasm preservation via the seeds. Dark-brown mature seeds could tolerate desiccation to less than 5% MC and could tolerate −20 and −80 °C. Seeds of kapok showed orthodox storage behavior. They can be stored at subzero temperatures with low MC for a long time. For light-brown seeds, germination percentage (GP), germination index (GI), seedling fresh weight (SFW), and vigor index (VI) decreased significantly after seed desiccation. Germination percentage of light-brown seeds with different MC increased to a different extent after being stored at different temperatures for 1 year (76% to 99%), compared with the fresh seeds (73%). Storing fresh seeds at 4 °C was most favorable to keep seed viability and seed vigor of light-brown seeds. Seed collection could be done several days earlier than the time of fruit burst to ensure increased quantity of collected seeds.

scholarly journals Germination of Osmotically Primed Asparagus and Tomato Seeds after Storage up to Three Months

1994 ◽  
Vol 119 (3) ◽  
pp. 636-641 ◽  
Author(s):  
Paul L. Owen ◽  
Wallace G. Pill
Keyword(s):  
Relative Humidity ◽  
Osmotic Stress ◽  
Storage Temperature ◽  
Germination Rate ◽  
Seed Viability ◽  
Germination Percentage ◽  
Asparagus Officinalis ◽  
Synthetic Seawater ◽  
Tomato Seeds ◽  
Storage Temperatures

The influence of two drying regimes and two storage temperatures of primed asparagus (Asparagus officinalis L.) and tomato (Lycopersicon esculentum Mill.) seeds on germination after storage up to 3 months was examined. Seeds of `Mary Washington' asparagus and `Ace 55' tomato primed in synthetic seawater (-1.0 MPa, 20C, 1 week, dark) were surface-dried at 20C and 50% relative humidity (RH) for 2 h (42% to 49% moisture) or dried-back at 20C and 32.5% RH for 48 h (moisture = 13% tomato and 22% asparagus). These and nonprimed seeds were stored in tight-lidded metal cans and heat-sealed plastic pouches at 4 or 20C for up to 3 months before germination at 20C. After 3-month storage, primed surface-dried asparagus seeds stored at 4C had greater germination percentage and rate than nonprimed seeds, surface-dried seeds stored at 20C, or primed dried-back seeds. Dried-back primed tomato seeds had higher germination percentage than surface-dried primed seeds after 2 or 3 months of storage, with storage temperature having no effect on germination perecentage or rate. In a further study, primed surface-dried and primed dried-back seeds stored at 4 or 20C for 1.5 months in sealed containers were germinated at 15, 25, or 35C under low (-0.05 MPa) or high osmotic stress (-0.4 MPa). Primed surface-dried asparagus seeds stored at 4C, compared to nonprimed seeds, surface-dried seed stored at 20C, or primed dried-back seeds, had greater germination percentage at 15 and 35C and low osmotic stress, and higher germination rate at 15 or 25C. Primed tomato seeds had greater germination percentage than nonprimed seeds only at 35C and low osmotic stress, and higher germination rate at 15 or 25C. Storage of primed tomato seeds at 4C rather than 20C increased germination rate at 15 or 25C, and increased germination percentage at 35C and low osmotic stress. For maximal seed viability and germination rate after 1.5 to 3 months of storage, primed asparagus and tomato seeds should be stored at 4C rather than 20C; however, asparagus seeds should be surface-dried, and tomato seeds should be dried-back.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Using Microwave Soil Heating to Inhibit Invasive Species Seed Germination

2017 ◽  
Vol 10 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Mélissa De Wilde ◽  
Elise Buisson ◽  
Nicole Yavercovski ◽  
Loïc Willm ◽  
Livia Bieder ◽  
...  
Keyword(s):  
Invasive Species ◽  
Soil Moisture ◽  
Seed Germination ◽  
Invasive Plant ◽  
Organic Matter Content ◽  
Seed Viability ◽  
Germination Percentage ◽  
Matter Content ◽  
Burial Depth ◽  
Experimental Conditions

Successful invasive plant eradication is rare, because the methods used target the adult stage, not taking into account the development capacity of a large seedbank. Heating by microwave was considered, because it offers a means to quickly reach the temperature required for loss of seed viability and inhibition of germination. Previous results were not encouraging, because homogeneous and deep-wave penetration was not achieved, and the various parameters that can affect treatment effectiveness were incompletely addressed. This study aimed to determine, under experimental conditions, the best microwave treatment to inhibit invasive species seed germination in terms of power (2, 4, 6 kW) and duration (2, 4, 8 min) of treatments and depending on soil moisture (10%, 13%, 20%, 30%) and seed burial depth (2, 12 cm). Three invasive species were tested: Bohemian knotweed, giant goldenrod, and jimsonweed. The most effective treatments required relatively high power and duration (2kW8min, 4kW4min, 6kW2min, and 6kW4min; 4kW8min and 6kW8min were not tested for technical reasons), and their effectiveness diminished with increasing soil moisture with germination percentage between 0% and 2% for the lowest soil moisture, 0% and 56% for intermediate soil moisture, and 27% and 68% in control treatments. For the highest soil moisture, only 2kW8min and 4kW4min reduced germination percentage between 2% and 19%. Occasionally, germination of seeds located at the 12-cm depth was more strongly affected. Giant goldenrod seeds were the most sensitive, probably due to their small size. Results are promising and justify further experiments before developing a field microwave device to treat large volumes of soil infested by invasive seed efficiently and with reasonable energy requirements. Other types of soil, in terms of texture and organic matter content, should be tested in future experiments, because these factors influence soil water content and, consequently, microwave heating.

scholarly journals Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction

2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Gabriel Streck Bortolin ◽  
Alfredo José Salinas-Arcienega ◽  
Yutcelia Carolina Galviz-Fajardo ◽  
Luciano do Amarante ◽  
Carlos Eduardo da Silva Pedroso ◽  
...  
Keyword(s):  
Seed Germination ◽  
Physiological Responses ◽  
Dry Mass ◽  
Germination Percentage ◽  
Early Growth ◽  
Paspalum Notatum ◽  
Water Restriction ◽  
Germination Test ◽  
Growth Activity ◽  
Higher Temperature

ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).

scholarly journals Mid storage seed hardening: a mechanical method to maintain seed viability during long term jute seed preservation

2021 ◽  
Vol 8 (1) ◽  
pp. 79-88
Author(s):  
Md. Nasir Uddin ◽  
S. M. Mahbub Ali ◽  
Md. Abu Sadat ◽  
Md Amazed Hossain Chowdhury ◽  
Israt Jahan Mumu ◽  
...  
Keyword(s):  
Crop Production ◽  
Agricultural Sector ◽  
Seed Viability ◽  
Early Period ◽  
Chemical Properties ◽  
Seed Storage ◽  
Germination Percentage ◽  
Oil Degradation ◽  
Hardening Treatment

Seed plays an important role in agricultural sector for both production and consumption purpose. Availability of vigour seed is one of the major constraints for maximizing crop production. However, healthy seed can also lose its viability during seed storage by changing different physio-chemical properties. Influence of environmental factors and seed containers during storage leading to seed deterioration. In this research, mid storage seed hardening treatment was applied in different aged seeds of jute species (C. Capsularis & C. olitorius) with two types of storage bags. Seed hardening treatment showed the less moisture content with better germination percentage compared to the untreated species of jute seeds. Seed packing in polythene bags during both short and long term seed storages had higher viable seeds compared to the cloth packing seeds. The effect of seed hardening treatment on seed oil content and pattern of oil degradation is distinct in early period of storage. The faster rate of oil degradation, soluble protein and free amino acids was found in seeds of un-treated stored seeds in cloth bag. Contrary, very slow rate of oil degradation was observed in harden seed and stored in polythene bag which indicated better storability of harden seeds.

scholarly journals THE EFFECT OF CORONA INCANDESCENT PLASMA RADIATION AND STORAGE TEMPERATURE ON THE VIABILITY OF GARLIC SEEDS (ALLIUM SATIVUM L. LUMBU KUNING VARIETY)

2021 ◽  
Vol 5 (1) ◽  
pp. 11-17
Author(s):  
Febiasasti Trias Nugraheni ◽  
Erma Prihastanti ◽  
Endah Dwi Hastuti
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Storage Temperature ◽  
Germination Percentage ◽  
Plasma Radiation ◽  
Agricultural Commodity ◽  
Time Duration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Storage

Garlic (Allium sativum L.) is an agricultural commodity which is widely consumed by the community. The obstacle experienced in cultivation is that a relatively long dormancy period of about 6-7 months. The dormancy of garlic seeds occurs because the embryo has not yet been fully formed, and the growth hormone and inhibitory hormone are not balanced. The methods used to break dormancy and accelerate germination are by using corona incandescent plasma radiation and storage temperature. The research utilized factorial Completely Randomized Design (CRD), consisting of six treatments with five replications. The first factor was the corona incandescent plasma radiation carried out on the garlic seeds ‘Lumbu Kuning’ variety with time duration of 0, 15, and 30 minutes. The second factor was storage temperature comprising of room temperature and cold temperature of 7oC stored for 30 days. Viability parameters observed are germination percentage dan the length of sprouts. The data were analyzed by using ANOVA followed by the DMRT test at the 95% confidence level. The results showed that corona incandescent plasma radiation influenced the germination percentage using plasma radiation for 15- 30 minutes.

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