Evaluation of Varieties of Caupi Bean to Meloidogyne Incognita Parasitism

Author(s):  
Gilberto Antonio Torres Júnior ◽  
Fernandes Antonio de Almeida ◽  
Erik Gomes Sampaio ◽  
Francisco Tarcísio Lucena ◽  
Maria Lucia Tiburtino Leite ◽  
...  

The culture of Cowpea (Vigna unguiculata L.) of wide adaptation of the various edaphoclimatic conditions of Brazil is of fundamental importance for the socioeconomic development of the north and northeast regions, however, the productivity can be limited by pathogenic agents, such as phytonematodes, belonging to the genus Meloidogyne. Due to this condition, the objective was to evaluate the behavior of different varieties of cowpea against the parasitism of M. incognita. The reaction test was performed with inoculation of 5.000 eggs/juveniles in the region of the root. The experiment was carried out in a greenhouse, with a completely randomized design, with 14 varieties (Bastiãozinho; Mosqueado; Maravilha; Balinha; Garanhão; Sempre verde; Canapuzinho; Canapu branco; Paulistinha; Pujante; Canapu da Vagem Roxa; Rajado PE; Costela de Vaca e Setentão) with five replications. 60 Sixty days after inoculation, some agronomic characteristics and parasitism were evaluated. All varieties were susceptible to M. incognita, with RR > 1. However, the Paulistinha variety proved to be moderately resistant.

2020 ◽  
pp. 1236-1241
Author(s):  
Gustavo H. Sera ◽  
Filipe G. Carvalho ◽  
Inês C. de B. Fonseca ◽  
Luciana H. Shigueoka ◽  
Santino A. da Silva ◽  
...  

The aim of this study was to prove that Arabica coffee introgressed with C. liberica, have resistance to Meloidogyne paranaensis (Mp). Open pollinated fruits were harvested from mother plants of 29 Arabica coffee genotypes from the IAPAR germplasm bank. Seeds were collected from the fruits and were sown to obtain seedlings to test the resistance to Mp. The experiment was set up in a completely randomized design with 29 coffee genotypes, 8 replications, and one plant per plot. Cultivars Catuaí Vermelho IAC 99 and IPR 100 were used as susceptible and resistant checks, respectively. Seedlings with three to four pairs of leaves were inoculated with 1,400 eggs and juveniles J2 of Mp (IP). At 120 days after inoculation, seedlings were evaluated by counting the nematodes per gram of roots, and the final nematodes population was obtained (FP). The reproduction factor (RF) was calculated using the formula: RF = FP/IP. The reproduction factor reduction was used to classify the resistance levels of genotypes, which were classified as highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) an highly susceptible (HS). All genotypes differed from Catuaí in resistance factor (RF), five of which did not differ from IPR 100 for RF, and only the line IAPAR 15242 had RF < 1.0. Out of 28 Arabica genotypes introgressed with C. liberica, five HR, 11 R, 11 MR and one MS were identified. However, only IAPAR 15242 and IPR 100 were classified as HR and presented 100% of HR plants, but only the first showed an RF < 1.0. Results revealed that these Arabica coffee genotypes with introgression of C. liberica genes have great potential to be used in breeding programs and they are a new alternative as a source of resistance.


2018 ◽  
Vol 1 (2) ◽  
pp. 38
Author(s):  
Ika Susanti Hendriyani ◽  
Yulita Nurchayati ◽  
Nintya Setiari

Chlorophyll is a pigment of photosynthesis which is also efficacious as a cure for brain, lung, and oral cancer, and can be used as a disinfectant, antibiotic and dietary supplement, while carotenoids are useful as antioxidants. The content of the two pigments is strongly influenced by the age of the plant, especially for vegetable crops used in leaves such as cowpea (Vigna unguiculata (L.) Walp.). The purpose of this research is to know the pattern of chlorophyll content and carotenoid of cowpea leaf during the growth phase and to know the age of the right plant for harvesting cowpea so that the highest chlorophyll and carotenoid content is obtained. Cowpea seeds obtained from seeds are added then planted in pots containing ready-made planting media in the greenhouse. Chlorophyll and carotenoid analyzes were performed at different plant ages from 1 to 11 weeks. The research design used is Completely Randomized Design. Data were analyzed using variance analysis and continued with Duncan test at 95% significance level. The results showed that the highest chlorophyll and carotenoid content was produced by the cowpea leaf in the vegetative phase or before the flowering time. The highest chlorophyll content was obtained at 4WAP (weeks after planting) of age crop, while the highest carotenoid content was obtained in 3WAP.  


2021 ◽  
Vol 34 (4) ◽  
pp. 957-964
Author(s):  
RISONEIDE DE CÁSSIA ZEFERINO SILVA ◽  
ANTHONY CARLOS DA SILVA ◽  
REZANIO MARTINS CARVALHO ◽  
ANTONIO FÉLIX DA COSTA ◽  
ALESSANDRO NICOLI ◽  
...  

ABSTRACT Fusarium wilt, caused by soil-borne Fusarium oxysporum f. sp. tracheiphilum (Fot), can reduce cowpea bean yield. Considering that genetic control through resistant genotypes is pivotal for Fusarium wilt control, the aim of the present study was to identify cowpea genotypes that are resistant to Fot from the Germplasm Bank of the Pernambuco Agronomic Institute, Embrapa Middle North, and other producing areas in the North and Northeast regions of Brazil. The cultivar BR-17 Gurguéia was used as a susceptible control and MNC01-649F-2-1 was used as a resistant control. Two experiments were carried out in a completely randomized design in the greenhouse of the Department of Agronomy of the University Federal Rural of Pernambuco. Plants with the first pair of expanded leaves were inoculated with a conidial suspension (106 conidia/mL) using the conidia root-immersion methodology. Fusarium wilt severity in plants was assessed 21 d after inoculation, with the aid of a descriptive scale. In the first experiment, we evaluated the resistance of 38 cowpea genotypes, of which 19 (5 moderately resistant and 14 resistant) were selected for the second experiment. In the first experiment, four genotypes (Canapu PE, Miranda IPA 207, Esperança, and BRS Pujante) did not show any symptoms of Fusarium wilt. In the second, 15 genotypes showed high resistance to Fot, including Canapu PE and Miranda IPA 207, which again had no symptoms of Fusarium wilt. In conclusion, this study identified that the genotypes, Canapu PE, Miranda IPA 207, Esperança, and BRS Pujante have greater resistance to Fusarium wilt.


2020 ◽  
Vol 13 (1) ◽  
pp. 30-37
Author(s):  
Ika Ratna Agustin ◽  
Yunianta ◽  
Teti Estiasih

Cowpea (Vigna unguiculata L. Walp) has a protein content of 22,9% to obtain the protein in high concentrations, is made as a concentrates or isolates protein. The aim of study to determine of chemical and functional properties of flour and cowpea protein concentrates on their solubility. The study uses a completely randomized design with precipitation method based on solvent, there is distilled water, 5% salt solution, alkaline solution (NaOH 0,5 N) and ethanol 70%. The result showed that cowpea protein fractions of albumin, globulin, glutelin and prolamin had different minimum and maximum solubility. Flour and cowpea protein concentrates had different chemical composition and functional properties. Flour and cowpea protein concentrates can be develop in various food product


2017 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Sri Nugroho Marsoem ◽  
Harry Feryanto ◽  
Hiroyuki Yamamoto

Sukun (Artocarpus communis FORST), also known as breadfruit, is a multipurpose tree and is mostly grown in community forest. In an effort to increase the added value of Sukun trees grown in community forest, a study on the cell proportion and dimension of the wood was conducted by using Sukun trees grown on Karangmojo Village, Bantul Regency, Yogyakarta. Samples for the study were taken from pith to bark in the north direction of the stem. Cell proportion as well as fiber dimension were then observed. The properties observed were analyzed by analysis of variance using a Completely Randomized Design. The result shows that the wood was composed of fiber of only 37.91%, while its rays, parenchyma, and vessel are of 26.60%, 23.85% and 11.61% respectively. The wood had a rather long average fiber length that is 1.65 mm and fiber diameter of 42.48 µm, lumen diameter of 37.28 µm and cell-wall thickness of 2.60 µm.


2012 ◽  
Vol 38 (1) ◽  
pp. 24-29 ◽  
Author(s):  
Leonardo Nazário Silva dos Santos ◽  
Fábio Ramos Alves ◽  
Leônidas Leoni Belan ◽  
Pablo Diego Silva Cabral ◽  
Frederico de Pina Matta ◽  
...  

The damage and the resistance levels of cultivars and accessions of common beans rescued in the South and mountain regions of Espírito Santo State, Brazil, to M. incognita race 3 and M. javanica parasitism were evaluated under a greenhouse. Four rescued bean genotypes ("FORT-10", "FORT-13", "FORT-16" and "FORT-19") and 2 commercial cultivars: "Pérola", and "Aporé", were tested. The cultivar "Rico-23" was included as standard of susceptibility to nematodes and non-inoculated plants constituted the control. Thus, the experiment was carried out in a completely randomized design in 3 (treatments considering nematodes) x 7 (genotypes and bean cultivars) factorial arrangement, with 7 replicates. Data were measured at 50 days after plant inoculation. For damage quantification, the following variables were evaluated: plant height (PHE), number of nodes (NNO), number of trifoliate leaves (NRT), fresh matter weight (FWE) and dry matter weight (DWE) of shoots, root weight (RWE), number of root nodules (NRO) and final population (FPO) of nematodes per root system. There were no significant differences between the effects caused by M. incognita and M. javanica, but both species showed inferior values of PHE, NNO, NRT, RWE, FWE and DWE compared to controls. Concerning the levels of resistance of bean plants to M. incognita, the genotypes "FORT-10", "FORT-13", "Aporé" and "FORT-16" behaved as moderately resistant, the cultivars "Rico 23" and "Pérola" low resistant, and the genotype "FORT-19" as highly susceptible. When parasitized by M. javanica, the beans "FORT-19", "Rico-23", "FORT-16" and "FORT-13" were low resistant, "Pérola" and "Aporé" susceptible and "FORT-10" highly susceptible.


2016 ◽  
Vol 46 (4) ◽  
pp. 413-420 ◽  
Author(s):  
Wéverson Lima Fonseca ◽  
◽  
Fernandes Antonio de Almeida ◽  
Augusto Matias de Oliveira ◽  
Maria Lúcia Tiburtino Leite ◽  
...  

ABSTRACT Manipueira, a liquid residue obtained from the cassava industrialization, shows high toxicity to the microbial diversity. This study aimed at evaluating the potential of manipueira applied to the soil to control Meloidogyne incognita in soybean. A completely randomized design, in a 2 x 11 factorial scheme, was used, consisting of two application forms of manipueira (single and two applications), in eleven concentrations (0 %, 10 %, 20 %, 30 %, 40 %, 50 %, 60 %, 70 %, 80 %, 90 % and 100 %), with five replications per treatment. Some agronomic traits and parasitism characteristics were also evaluated. The plants that received a single application of manipueira showed a gain of 100.41 % in root length, while the volume and fresh root mass showed gains of 81.52 % and 28.11 %, respectively, with the two applications. Regarding parasitism, the single application was more effective in reducing the number of juveniles in the soil and roots, where the concentrations of manipueira to kill 50 % of the nematodes were 1.65 % and 4.37 %, respectively. Thus, besides being effective in controlling M. incognita, manipueira has a positive effect on the development of soybean and may be recommended as a nematicide and also as an organic fertilizer.


2019 ◽  
Vol 49 ◽  
Author(s):  
João Pedro Gomes da Costa ◽  
Pedro Luiz Martins Soares ◽  
Roberta Luiza Vidal ◽  
Daniel Dalvan do Nascimento ◽  
Rivanildo Ferreira Junior

ABSTRACT The production of common bean (Phaseolus vulgaris L.) may be compromised by a variety of pests and diseases, with root-knot nematodes standing out among the soil-borne pathogens that cause significant losses. It was assessed the reaction of common bean genotypes to the reproduction of these nematodes, with emphasis on the two species most frequently found parasitizing the crop: Meloidogyne javanica and Meloidogne incognita. The experiments were conducted in a greenhouse, using a completely randomized design, with 26 treatments (genotypes), ten replications and plot consisting of a pot containing one plant. Each plot was inoculated with 1,000 eggs and second-stage juveniles and assessed at 62 days after the inoculation, when the final populations were estimated. The genotypic resistance was determined based on two criteria: reproduction factor and index. All the genotypes were susceptible to M. incognita according to both assessment criteria. The IAC Alvorada, IAC Imperador, BRS Esplendor and BRS Esteio cultivars, although may be classified as resistant to M. javanica by the reproduction factor, are classified only as moderately resistant if the assessment criterion is the reproduction index.


Author(s):  
U. C. M. Pessôa ◽  
E. N. Silva Terceiro ◽  
A. S. Souza ◽  
A. A. Soares Filho ◽  
T. A. Pimenta

<p>As plantas daninhas interferem no crescimento e desenvolvimento da cultura do feijão-caupi, podendo reduzir drasticamente a sua produtividade. Objetivou-se com o trabalho, avaliar a interferência de <em>Cyperus rotundus</em> e da compactação do solo no crescimento do feijão-caupi [<em>Vigna unguiculata</em> (L.) Walp]<ins cite="mailto:Bruno%20Anacleto" datetime="2016-03-01T10:35"> </ins>no sertão paraibano. O experimento foi realizado em estufa agrícola no Centro de Ciências e Tecnologia Agroalimentar da Universidade Federal de Campina Grande, CCTA/UFCG, Campus de Pombal-PB. As unidades experimentais foram compostas por vasos com capacidade de 6 litros. Utilizou-se o delineamento inteiramente casualizado com tratamentos distribuídos em esquema fatorial 3 x 2 , sendo os fatores três populações de <em>C. rotundus</em> (0, 2 e 3 tubérculos por vaso) e dois níveis de compactação (solo sem compactação e solo com subsuperfície compactada), com quatro repetições. Foram coletados os dados de produção de fitomassa, área foliar, altura da planta, número de folhas e diâmetro do caule, os quais foram submetidos à análise da variância e teste de médias. A presença de <em>C. rotundus</em>, independente de sua população prejudicou os componentes de crescimento, reduzindo a área foliar, de forma mais intensa do que a compactação do solo. Com isto, urgem medidas que reduzam os prejuízos causados por <em>C. rotundus </em>a cultura do<em> </em>feijão-caupi.</p><p align="center"><strong><em>Interference of nut grass (</em></strong><strong><em>Cyperus rotundus L.)</em></strong><em> </em><strong><em>and soil compaction on cowpea growth</em></strong></p><p><strong>ABSTRACT</strong> - Weeds interfere with the growth and development of the cowpea crop and can dramatically reduce their productivity. The objective of this study was to evaluate the interference of nut grass and soil compaction in the growth of cowpea [<em>Vigna unguiculata</em> (L.) Walp]. The experiment was conducted in an agricultural greenhouse in the Science and Technology Center of Agrifood Federal University of Campina Grande, CCTA/UFCG, Campus de Pombal-PB, Brazil. The experimental were composed of pots with 6 liters capacity. Used a completely randomized design with treatments distributed in a factorial 3 x 2 x 4, comprising the three populations of <em>C. rotundus</em> (0, 2 and 3 tubers per pot) and two compression levels (soil without compaction and soil compacted subsurface), with four replications. Were collected biomass production data, leaf area, plant height, leaf number and stem diameter, which were submitted to analysis of variance and mean test when necessary. The presence of <em>C. rotundus</em>, regardless of its population hampered growth components, reduced leaf area, more intensely than soil compaction. With this, urge measures to reduce the harm caused by <em>C. rotundus</em> the cowpea.</p><p> </p>


Nativa ◽  
2018 ◽  
Vol 6 (6) ◽  
pp. 608
Author(s):  
Eveline Nogueira Lima ◽  
Kátia Regina Alves Pires ◽  
Elaine Facco Celin ◽  
Cândida Hermínia Campos de Magalhães Bertini ◽  
Rosilene Oliveira Mesquita

Objetivou-se com esse trabalho identificar genótipos de feijão-caupi tolerantes e suscetíveis ao déficit hídrico, utilizando-se duas metodologias, estresse hídrico simulado com o uso de PEG6000 (Polietilenoglicol) e Screening Box. Os experimentos foram conduzidos em casa de vegetação no delineamento inteiramente casualizado com três repetições. Para a metodologia com PEG6000 foram utilizados 15 genótipos e duas testemunhas, com três repetições, totalizando 90 amostras. Foram selecionadas seis plântulas por genótipo, utilizaram-se três caixas contendo apenas água destilada e outras três com nível de potencial osmótico de - 0,2 MPa de PEG6000. A avaliação foi feita até atingir o ponto máximo de crescimento das raízes. Para a metodologia de Screening Box foram utilizados 15 genótipos, onde estes foram avaliados quanto à tolerância ao déficit hídrico no estádio de plântula. As duas metodologias mostraram-se eficientes na seleção de genótipos de feijão-caupi, os genótipos Santo Inácio Vermelho e BRS Tumucumaque, foram os mais sensíveis à seca, em ambas as metodologias.Palavras-chave: Vigna unguiculata, seca, plântulas. COWPEA GENOTYPES TOLERANT AND SUSCEPTIBLE TO WATER DEFICIT ABSTRACT: Objective to identify tolerant and susceptible cowpeas genotypes to water deficiency at the seedlings stage of growth, using two methodologies such as (a) water stress with the use of PEG6000 (Polyethylene glycol) and the Screening Box were used in the present research. The experiments were conducted in greenhouse environments using a completely randomized design with three replications. For the PEG6000 approach, 15 genotypes and two controls treatments were used in three replications in a total of 90 samples. Six seedlings were selected per genotype by using three boxes containing only distilled water. In the other treatment, three level of osmotic potential of -0.2 MPa of PEG6000. The evaluations of the trials were done until the Screening Box approach, 15 genotypes were used and were evaluated for tolerance to water deficit at the seedling stage of growth. The two methodologies were efficient in the selection of cowpea genotypes, the genotypes Santo InácioVermelho and BRS Tumucumaque were the most sensitive to drought in both methodologies.Keywords: drought, Vigna unguiculata, seedlings.


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