scholarly journals Phytochemical Screening and Hepatoprotective Potential of Sida cordata

2021 ◽  
pp. 452-459
Author(s):  
Sunil Mistry
Keyword(s):  
Biochemical Markers ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Histological Structure ◽  
Phytochemical Screening ◽  
Significant Elevation ◽  
Quality Estimation ◽  
Histological Changes ◽  
Hepatic Markers

Sida cordata (Burn. f.) Borss. Waalk is highly distributed in entire part of India. The various parts of the plant are used in folklore medicine for the management of multiple diseases.  So,the present study was performed to examine the  hepatoprotective activity study of ethanolic extract of S. cordata leaves (SCLE) on alcohol mediated  hepatotoxicity in a rat model. Ethanol intoxicated rats showed noteworthy elevation of liver weight and volume. Further, ethanol insulted rats also showed significant elevation in the level of biochemical markers such as AST, ALT, TG, TB, and DB) and marked variation in the histological structure of liver. Oral administration of SCLE at the dose of 200 and 400 mg/kg for 30 days) significantly reduced the liver weight, hepatic markers level and restored the histological changes annoyed by ethanol thus indicating its hepatoprotective potential. Furthermore, HPTLC analysis was performed for the identification and quality estimation of S. cordata leaves.

scholarly journals Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7353
Author(s):  
Nasir Aziz Wagay ◽  
Shah Rafiq ◽  
Mohammad Aslam Rather ◽  
Younas Rasheed Tantray ◽  
Feng Lin ◽  
...  
Keyword(s):  
Total Protein ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Experimental Models ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Total Lipids ◽  
Anti Inflammatory

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.

scholarly journals Isolation, Characterization of Active Constituent and Evaluation of Hepatoprotective Activity of Inula Racemosa Hook. F. Roots

2021 ◽  
Vol 37 (5) ◽  
pp. 1199-1204
Author(s):  
Vivek Kumar ◽  
Harpreet Singh ◽  
Arun Kumar Mishra
Keyword(s):  
Gallic Acid ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Protective Effects ◽  
Active Constituent ◽  
Inula Racemosa ◽  
First Time

Ayurvedic literature claims that Inula racemosa Hook. f. roots are beneficial for the liver. The study’s aim was to test the hepatoprotective effect of Inula racemosa Hook. f. roots ethanolic extract (IRE) against paracetamol (PCM) induced hepatotoxicity in rats. Silymarin (100 mg/kg/day) was used for 7 days, then PCM was orally administered (3 g/kg b.wt.) on the eighth day. 24 hours after the last PCM dosing, blood was withdrawn from the retro-orbital plexus and later on the rats were sacrificed. Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), Albumin (ALB), Total Protein (TP), liver weight and relative liver weight were determined. Histopathology of the liver was performed. In the study, IRE was found to have comparable protective effects against PCM-induced hepatotoxicity as Silymarin. Gallic acid was isolated for the first time from Inula racemosa Hook. f. roots. IRE exhibited a hepatoprotective effect because gallic acid was present in it.

Phenolic Profile, Acute Toxicity, Hepatoprotective and Antiproliferative Activities of Algerian Ruta tuberculata forssk

2021 ◽  
Vol 17 ◽  
Author(s):  
Asma Saidi ◽  
Leila Hambaba ◽  
Burak Kucuk ◽  
Ercan Cacan ◽  
Ramazan Erenler
Keyword(s):  
Acute Toxicity ◽  
Antiproliferative Activity ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Phenolic Profile ◽  
Histological Changes ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Oral Acute Toxicity ◽  
Hepatic Markers

Objective: Ruta tuberculata forssk. (Rutaceae) is an aromatic plants widely used in Algerian traditionally medicine due to its pharmaceutical virtues against various disorders. This study aims to determine the phenolic profile of aqueous (RAE) and methanol (RME) extracts of R. tuberculata aerial parts and to investigate their acute oral toxicity, as well as their possible antiproliferative and hepatoprotective effects. Methods: Polyphenols were identified by quantitative LC-MS/MS analysis. Oral acute toxicity was performed according to OCDE guidelines. The hepatoprotective activity was evaluated by paracetamol-induced hepatotoxicity and supported by biochemical and histological analysis of liver and kidneys. The antiproliferative activity against human colorectal HT-29 and ovarian OV2008 cancer cell lines was determined using SRB assay. Results: LC-MS/MS analysis revealed that RME has higher phenols and flavonoids content than RAE, however, it’s major identified flavonoids namely Kaempferol, rutin and naringenin. R. tuberculata seems mildly toxic at several doses, with oral LD50 greater than 5000 mg/kg. the significant increase in hepatic markers enzymes activities as well as cholesterol, triglycerides and glycemia levels, caused by PCM-administration, was potentially reduced following the co-treatments with vitamin C and RME, respectively, compared to RAE. Moreover, RME-treatment markedly prevented all histological changes. Compared to RAE, RME (100 μg/mL) exhibited excellent antiproliferative activity against both tested cancer lines (% inhibition ≥ 80 %). Conclusion: Both R. tuberculata extracts (200 mg/kg/daily) were non-toxic and exerted a potential hepatoprotective effect against PCM-induced hepatotoxicity. Accordingly, RME may be considered a good candidate for the development of new therapies against colorectal and ovarian cancers.

scholarly journals Evaluation of Hepatoprotective and Antioxidant Activity of Ethanolic Extract of Artabotrys zeylanicus Stem against Various Hepatotoxins Induced Hepatotoxicity in Albino Wister Rats

2021 ◽  
pp. 25-37
Author(s):  
K. Suresh ◽  
Hindustan Abdul Ahad ◽  
S. V. Satyanarayana
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Markers ◽  
Oxidant Stress ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Glutamic Pyruvic Transaminase ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Ic50 Value ◽  
Stem Extract

The objective of the present study was to investigate the antioxidant and hepatoprotective activity of ethanolic stem extract of Artabotrys zeylanicus against paracetamol (PCT), Ethanol (ETN) and Isoniazid and Rifampicin (IR) induced hepatotoxicity in Albino wister rats. Methodology: The material was dried in shade, they were powdered and extracted with ethanol. Preliminary Phytochemical tests were done. The hepatoprotective activity of the ethanol extract was assessed in Albino wister rats. PCT (3 g/kg), ETN (5 gm/kg) and IR (100 mg/kg) has enhanced the levels of various biochemical markers of hepatic damage like Serum Glutamic Oxaloacetic Trasaminase (SGOT), Serum Glutamic pyruvic transaminase (SGPT), Alkaline phosphatise (ALP), bilirubin. Antioxidant levels were tested in all the Hepatotoxins treated and untreated groups. Results: The various biochemical and Histopathological investigations done were Serum Glutamic Oxaloacetic Trasaminase (SGOT), Serum Glutamic pyruvic transaminase (SGPT), Alkaline phosphatise (ALP), Bilirubin, antioxidant activity by 1,1-diphenyl 2-picryl hydrazyl (DPPH), Nitro Blue Tetrazolium (NBT), Hyderogen peroxide (H2O2), lipid perioxidation, hyderoxil radical and nitric oxide. Treatment of ethanolic extract of stem of A. zeylanicus (100mg/kg, 200mg/kg and 400mg/kg body weight) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner. Ethanolic extract of A. zeylanicus were observed to inhibit oxidant stress with the maximum value of 71% and 62% at the concentration of 100 µg/mL. The crude ethanolic extract of A. zeylanicus had a calculated IC50 value of 62.2 and 63.25 μg/mL, which is nearly similar to the calculated IC50 value of the known antioxidant, ascorbic acid, ie 65.3 μg/mL. While the rats treated with AZ extract (100, 200 and 400 mg/kg) which were shown as reduction/absence of inflammatory cells, vascular congestion, cellular degeneration, necrosis and vacuoles. In contrast, the lower doses (100 mg/kg) of ethanolic extract of AZ stem shown low protection than at higher dose 400 mg/kg. Conclusion: Our findings suggested that A. zeylanicus ethanol stem extract possessed a potent antioxidant and hepatoprotective activity.

scholarly journals Evaluation of Liver Protective Activity of Moringa oleifera Bark Extract in Paracetamol Induced Hepatotoxicity in Rats

2019 ◽  
pp. 1-9
Author(s):  
Rajibul Islam ◽  
Md. Jahir Alam ◽  
Sara Benojir Shanta ◽  
Md. Habibur Rahman ◽  
Sultan Mahmud ◽  
...  
Keyword(s):  
Body Weight ◽  
Moringa Oleifera ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Bark Extract ◽  
Once Daily ◽  
Injury Recovery ◽  
Hepatoprotective Drug

Background: Moringa oleifera has been used in folk medicine to alleviate several diseases. In the present study, ethanolic extract of Moringa oleifera bark has been investigated to study its potential on paracetamol induced hepatotoxicity on model rats. Methods: Rats (150–200 gm) were divided into 5 groups containing 6 animals each. Acute hepatotoxicity was induced by paracetamol (600 mg/kg body weight) administered once daily for one week whereas the extract of investigated plant was given orally throughout the whole experiment at 250 and 500 mg/kg body weight. Silymarin (100 mg/kg body weight) was given orally as standard hepatoprotective drug. The level of hepatic injury recovery was determined by the estimation of liver enzymes like SGPT, SGOT, ALP, Bilirubin, Total protein, globulin and Albumin. Results: Treatment with MO extract as well as standard hepatoprotective agent silymarin ameliorated plasma levels of hepatic enzymes. Body weight was improved significantly by MO extracts (p < 0.01), whereas liver weight was recovered insignificantly. SGPT, SGOT and ALP levels were improved very highly significantly (p<0.001) and highly significantly (p<0.01) at MO 250mg dose. While at the dose of 500 mg/kg ameliorated SGPT Level very highly significantly (p<0.001), SGOT Level highly significantly (p<0.01) but insignificant to ALP level.   Conclusion: The biochemical parameters provide evidence that the ethanolic extract of of Moringa oleifera bark has shown hepatoprotective activity.

Biological and Phytochemical Screening of Tephrosia purpurea extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Marker Enzymes ◽  
Tephrosia Purpurea ◽  
Single Intraperitoneal Injection ◽  
Chemically Induced ◽  
Ccl4 Treatment ◽  
Dose Dependent

Tephrosia purpurea possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Tephrosia purpurea would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Tephrosia purpurea is very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of aTephrosia purpurea of 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt.Tephrosia purpureaextract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Tephrosia purpurea extract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats.

Biological and Phytochemical Screening of Fumaria indica extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao2 ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Liver Disease ◽  
Liver Cancer ◽  
Early Stage ◽  
Food Additives ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Industrial Chemicals ◽  
Toxic Industrial Chemicals

Liver disease or liver cancer is the sixth most common cancer and the third leading cause of cancer mortality in the world. Hepatitis viral infection, food additives, alcohol, fungal toxins (aflatoxins), toxic industrial chemicals, air and water pollutants are the major risk factors of liver cancer. Moreover, due to high tolerance of liver, HCC is seldom detected at an early stage and once detected treatment faces a poor prognosis in most cases.Fumaria indica possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Fumaria indica would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Fumaria indicais very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of a Fumaria indicaof 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt. Fumaria indica extract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Fumaria indicaextract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats. In addition to this, studies on molecular aspect of hepatoprotective therapy will give mechanistic information in hepatoprotective therapy and also critical balance should be there between the animal model and clinical research. The hepatoprotective properties of Fumaria indicashould provide useful information in the possible application in hepatic liver disease.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

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