scholarly journals Histopathological Changes on Testes, Liver, Kidney and Brain Tissues in Acute Boric Acid Administration

2021 ◽  
pp. 337-346
Author(s):  
Dursun Figen Emeksiz Ayranci ◽  
Unal Ozelmas ◽  
Unal Ayranci
Keyword(s):  
Light Microscopy ◽  
Boric Acid ◽  
Testicular Tissue ◽  
Average Weight ◽  
Histopathological Changes ◽  
Toxic Dose ◽  
Thin Sections ◽  
Experimental Group ◽  
Acid Administration ◽  
Brain Tissues

Introduction: In recent years as a result of the observation that the toxic effects of boron and its products have increased intensive studies have been initiated in our country and in the world regarding its effects, especially in the central nervous system, digestive system and reproductive system. The aim was to determine the histopathological changes caused by boric acid in rat testis, liver, kidney and brain tissues by light microscopy after oral administration of toxic dose of acute boric acid. Material and Methods: In the study, 1000 mg/kg/day boric acid was given orally for 7 days to 12-week-old 30 male albino SpragueDawley rats in total with an average weight of 285 g. Twelve male albino Sprague-Dawley rats of approximately the same weight and age were used as controls. At the end of the seventh day testes, liver, kidney and brain tissues were isolated from the animals. Results: At the end of the experiment, it was determined that the experimental group had significant body weight loss compared to the control group. Likewise, testicular, liver and kidney weights of the experimental group were decreased compared to the controls. In the histopathological examination performed with light microscopy in the testis, liver, kidney and brain tissues taken, congestion in the vascular bed of the testicular tissue and cellular degeneration at different rates were observed in paraffin sections and semi-thin sections. Conclusion: It was observed that acute boric acid administration, together with its widespread toxic effect, caused histopathological changes by inhibiting spermatogenesis, especially in testicular tissue.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Ectodesmata as Revealed By Freeze-Etch Preparations of Plant Epidermal Cell Walls

1973 ◽  
Vol 31 ◽  
pp. 468-469
Author(s):  
N.C. Lyon ◽  
W. C. Mueller
Keyword(s):  
Electron Microscopy ◽  
Acetic Acid ◽  
Nitric Acid ◽  
Oxalic Acid ◽  
Light Microscopy ◽  
Epidermal Cell ◽  
Cell Walls ◽  
Plant Viruses ◽  
Plant Leaves ◽  
Thin Sections

Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to saturation 2-3 g). Other workers have published electron micrographs of structures transversing the outer epidermal cell in thin sections of plant leaves that have been interpreted as ectodesmata. Such structures are evident following treatment with Hg++ or Ag+ salts and are only rarely observed by electron microscopy. If ectodesmata exist without such treatment, and are not artefacts, they would afford natural pathways of entry for applied foliar solutions and plant viruses.

Toxic effects of sub lethal concentration of dioctyl phthalate on the histology of liver of Clarias batrachus (Linn.).

2016 ◽  
Vol 5 (09) ◽  
pp. 4874
Author(s):  
Manisha Satpathi* ◽  
Ravinder Singh
Keyword(s):  
Acute Toxicity ◽  
Light Microscopy ◽  
Dioctyl Phthalate ◽  
Clarias Batrachus ◽  
Fresh Water Fish ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Water Fish ◽  
Toxicity Effects ◽  
Plastic Products

Dioctyl phthalate (DOP) commonly used as plasticizer enters into aquatic environment from the industries manufacturing plastic products, PVC resins, cosmetics and many other commercial products. Therefore, this study was designed to evaluate the acute toxicity effects of Dioctyl phthalate on fresh water fish Clarias batrachus. The 96 hour LC50 of Dioctyl phthalate in Clarias batrachus was estimated as 14.5ml/L. Histopathological changes in liver of Clarias batrachus were determined by exposing them to a fraction of LC50(1/5th) dose with every change of water for 30 days.The liver of Clarias batrachus was excised at every interval of 30 days and histological changes of liver were studied under light microscopy. Liver tissues showed abnormalities. Centrilobular vacuolation, necrosis, eccentric nuclei and enlarged nuclei, Centrilobular degeneration of hepatocytes were observed in liver tissue of fish.

Consequences of Microwave oven Exposed Diet on The Basal Lamina of Seminiferous Tubules of Mice

2021 ◽  
Vol 15 (8) ◽  
pp. 2141-2144
Author(s):  
Kishwar Naheed ◽  
Muhammad Saad Abdullah ◽  
Maria Yousaf ◽  
Humaira Ali ◽  
Fareeha Mushtaq ◽  
...  
Keyword(s):  
Basal Lamina ◽  
Treated Group ◽  
Testicular Tissue ◽  
Microwave Oven ◽  
Mentha Piperita ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Protective Effects ◽  
Trial Methodology ◽  
Experimental Group

Usage of electronic gadgets like microwave oven is increasing day by day that heats the food by exposing it to electromagnetic radiations which has many hazardous effects on human health including fertility. Aim: To find the effects of microwave oven exposed diet on basal lamina of seminiferous tubules of mice alongwith protective effects of Mentha piperita and melatonin on the same tissue. Study Design: Randomized control trial. Methodology: Adult male mice (n=32) were divided into four groups. Control group (G1) received standard pellets prepared for mice. Second group (G2) was given mice pellets exposed to microwave oven. Third group (G3) received Mentha Piperita leaf extract along with mice pellets exposed to microwave oven and the fourth group (G4) received oral melatonin along with pellets exposed to microwave oven. Later their testicular tissue was removed for histological examination while basal lamina disruption was assessed by scoring. Data analyzed by SPSS 22.0v. Results: In group G2, there was slight disruption in the basal lamina in 75% of the cases while in experimental group G3, there was slight disruption of basal lamina only in 12.5% of the cases. However, in group G4, only 25% specimen had slight disruption of basal lamina Conclusion: It was concluded that microwave oven exposed diet produced severe disruption of basal lamina in group G2 that decreased in Mentha piperita and melatonin treated groups. However, Mentha piperita treated group produced better results than melatonin treated group. Keywords: Mice, Testis, Basal Lamina, Mentha piperita and Melatonin

scholarly journals Histomorphological effect of ascorbic acid on mercury chloride-induced changes on the cerebellum of adult wistar rats

2014 ◽  
Vol 31 (04) ◽  
pp. 219-224 ◽  
Author(s):  
A. Ibegbu ◽  
A. Animoku Abdulrazaq ◽  
Ayuba Micheal ◽  
Brosu Daniel ◽  
A. Adamu Sadeeq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cerebellar Cortex ◽  
Wistar Rats ◽  
Oral Route ◽  
Mercury Chloride ◽  
Average Weight ◽  
The Central Nervous System ◽  
Living Organisms ◽  
Induced Changes ◽  
Acid Administration

Abstract Introduction. Mercury is one of the most hazardous environmental contaminants to living organisms and the central nervous system has been shown to be the main target. Objective. The present work was aimed at evaluating the effect of ascorbic acid on mercury chloride-induced changes on the cerebellar cortex of adult Wistar rats. Material and method. Thirty Wistar rats of average weight of 200g and were randomly divided into 6 groups of 5 rats each. The animals in Group 1 (control) were administered with distilled water, Groups 2 and 3 were administered with 52mg/kg and 26.25mg/kg body weight of HgCl respectively while Groups 4 and 5 were administered with 52mg/kg of HgCl and 5mg/kg of ascorbic acid and 26.25gm/kg of HgCl and 5mg/kg of ascorbic acid respectively, while Group 6 was administered with 5mg/kg of ascorbic acid. The administration was through oral route, daily for 3 weeks. Results. The result of the biochemical parameters showed a significant increase (P < 0.05) on the mean SOD and LPO values after the administration of mercury chloride and Ascorbic acid. Histological observation of the cerebellar cortex, showed normal histo-morphology in Groups 1 and 6 while, the cerebellum in Groups 2, 3, 4 and 5 showed some degenerative, necrotic and cellular changes. Conclusion. However, ascorbic acid administration has shown to ameliorate the induced degenerative changes in the cerebellum caused by mercury chloride toxicity in Wistar rats.

Doping and effects of anabolic androgenic steroids on the heart: histological, ultrastructural, and echocardiographic assessment in strength athletes

2009 ◽  
Vol 28 (5) ◽  
pp. 273-283 ◽  
Author(s):  
NA Hassan ◽  
MF Salem ◽  
Mael Sayed
Keyword(s):  
Light Microscopy ◽  
Strain Rate ◽  
Anabolic Steroids ◽  
Negative Control ◽  
Anabolic Androgenic Steroids ◽  
Control Group ◽  
Albino Rats ◽  
Two Dimensional ◽  
Androgenic Steroids ◽  
Experimental Group

Anabolic androgenic steroids (AAS) are used by some athletes to enhance performance despite the health risk they may pose in some persons. This work was carried out to evaluate the possible structural and functional alterations in the heart using two-dimensional, M-mode, tissue Doppler imaging (TDI) and strain rate imaging (SRI) in athletes using supraphysiological doses of AAS. Additionally, the histological and ultrastructural changes in cardiac muscles of adult albino rats after injection of sustanon, as an example of AAS, were studied. Fifteen male bodybuilders using anabolic steroids constituted group 1, five male bodybuilders who are not using anabolic steroids constituted group 2, and five nonathletic males constituted negative control group (group 3). They were investigated by two-dimensional, M-mode, TDI and SRI. This study was performed on 30 adult albino rats. They were divided into two groups. Group I (Control group) (10) was subdivided into negative control, subgroup 1a (5), and subgroup 1b (5), which received 0.8 ml olive oil intramuscular once a week for 8 weeks. Group II (Experimental group) (20) received sustanon 10 mg/kg intramuscularly once a week for 8 weeks. The heart specimens were prepared for light microscopy and transmission electron microscopy. Echocardiographic results showed that bodybuilders who use steroids have smaller left ventricular dimension with thicker walls, impaired diastolic function, as well as higher peak systolic strain rate in steroid-using bodybuilders as compared to the other two groups. Light microscopy examination of cardiac muscle fibers showed focal areas of degeneration with loss of striations and vacuolation in the experimental group. Ultrastructural examination showed disturbance of the banding pattern of the cardiac muscle fiber with disintegration, loss of striations, dehiscent intercalated disc, and interrupted Z-bands. Administration of supraphysiological doses of AAS caused severe deleterious effects in the myocardium both in athletes and in experimental animals. The SRI shows promise in the early detection of systolic dysfunction in those athletes who use steroids.

scholarly journals Research on the Influence of the Light and Sexing on Growth Performance and Slaughter Results at Young Quails from Mixed Baloteşti Population of Eggs-Meat

2017 ◽  
Vol 74 (1) ◽  
pp. 15
Author(s):  
Lucian IONITA ◽  
Elena POPESCU-MICLOSANU ◽  
George NICA ◽  
Cornel Octavian PANA
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Conversion Rate ◽  
Live Weight ◽  
Control Group ◽  
Average Weight ◽  
Feed Conversion ◽  
Feed Conversion Rate ◽  
Average Weight Gain ◽  
Experimental Group

The purposes of determining the influence of the light and sexing on growth performance and slaughter results atyoung quails from Balotesti population, was organized an experiment on 150 quails, betwen age of 28 and 70 days. The chicks were divided into two groups (control and experimental), and growth and slaughter performance were determined separately by sex. Have studied 2 lighting programs, a program of continous lighting with photoperiod duration of 16 hours (16 L + 8 N, control group) and a program with lighting with asymetric hours intervals with photoperiod duration of 16 hours (10L+2N+6L+6N, experimental group).Males and females from the experimental group were recorded average performance of growth high during the period 28 - 70 days. Live weight at age of 70 days was higher with 7.54 %, average weight gain was higher with 14.86 % and feed conversion rate was lower with 13.51 % at males from experimental group compared to males from the control group. Also, the live weight at the age of 70 days was higher with 18.09 %; average weight gain was higher with 35.12 %, while feed conversion rate was reduced with 30.08 % on females from the experimental group compared to females from the control group.The results of the slaughter at 70 days were superior in the caseof females from the both groups versus males. The average weight of the carcase was higher with 17.53 % in case of the females than that of males from the experimental group, and the carcase yield was 65.37 % at the females and 65.56 % for males. Also, the average weight of the carcase was higher with 5.08 % in case of females compared to that males from the control group, and the carcase yield was 64.20 % to females 66.70 % on males.Superior results recorded in the case of females and males from the experimental group recommended the program of lighting with asymmetric hours intervals in the growth of youth quail. Also, the higher slaughter results recorded in the case of females of the two groups recommended them in the meat production through a broiler of quail that combine advantages in the production of both males and females. 

scholarly journals Efficiency of zeolite basis preparation in fattening lambs diet

2012 ◽  
Vol 28 (3) ◽  
pp. 545-552 ◽  
Author(s):  
J. Stojkovic ◽  
Z. Ilic ◽  
S. Ciric ◽  
B. Ristanovic ◽  
M.P. Petrovic ◽  
...  
Keyword(s):  
Natural Zeolite ◽  
Dry Matter ◽  
Test Group ◽  
Daily Weight Gain ◽  
Control Group ◽  
Average Weight ◽  
Sheep Milk ◽  
The Impact ◽  
Experimental Group ◽  
At Will

The paper presents the results of a research on the impact of products based on natural zeolite on the production results of fattening lambs. The experiment involved two groups of lambs (the control - C and experimental - E groups), each consisting of 15 heads, for a period of 90 days. The meal was made from sheep milk, feed mixtures for fattening lambs and meadow hay. The test group lambs, unlike the ones from the control group, were given mixtures based on natural zeolite. Min-a-Zel S mixture (in the form of 25% composite) was fed to lambs from birth till their 14th day of life, directly into the mouth, once a day (before the morning feed), in the amount of 10 ml. Min-a-Zel Plus was given to them from their 15th day of life, together with the feed mixture (0.5%). Feeding was at will. The average weight of lambs at the end of the experiment, in accordance with the sequence of treatments (C:E) was 24.40:26.94 kg (P <0.01). Daily weight gain of lambs, during the experiment, was 229:256 g and in the experimental group it was by 27g or 11.79% higher (P <0.01). The test group lambs had a better utilization of dry matter, protein and energy, which justifies the use of products based on natural zeolite in fattening lambs.

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