scholarly journals Antioxidant and Anti-Cancer Effect of Ethanolic Extract of Citrus Fruits on Hep G2 and MCF-7 Cell Lines

2021 ◽  
pp. 84-90
Author(s):  
Shahid Rasool ◽  
Hammad Ahmed ◽  
Mehreen Malik Uttra ◽  
Ambreen Malik Uttra ◽  
Muhammad Rizwan Khan ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Citrus Sinensis ◽  
Radical Scavenging ◽  
Maximum Effect ◽  
Citrus Reticulata ◽  
Citrus Fruits ◽  
Dpph Method ◽  
Anti Cancer ◽  
Citrus Peels

Background/objective: Cancer is a complex genetic disease that occurs due to mutation in genes that control apoptosis and cell growth. Uncontrolled cell growth leads to the formation of tumors. Free radical causes mutation in genes and DNA sequence, however antioxidants can stabilize these harmful effects. Citrus fruits are the rich source of antioxidants. owing to this property this study was planned to evaluate the potential of the citrus fruits for the treatment of the cancer. Aim: This study aimed to evaluate antioxidant as well anticancer potential of five different citrus strains (Citrus deliciosa, Citrus maxima, Citrus limetta, Citrus sinensis and Citrus reticulata). Methods: Peel of all the citrus fruits were collected, grinded and ethanolic extracts were prepared separately, to evaluate radical scavenging ability by employing DPPH method followed by MTT assay of the cancer cell lines to explore the anticancer potential of the extracts. Results: It was observed that citrus peels exhibited good radical scavenging activity and inhibited tumor growth. Maximum effect was produced by Citrus reticulata, and least results were obtained with Citrus sinensis. Conclusion: It was concluded that antioxidant and anti-cancer effects of citrus peels may be due to be owing to the presence of antioxidants (ascorbic acid, flavonoids, phenols, limonene). This research might open new horizon in the treatment of cancer chemotherapy.

scholarly journals Composition Analysis and Anti-Cancer Properties of Two Endemic Phlomis Species (Phlomis cypria Post and Phlomis brevibracteata Turrill) from Cyprus

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 29
Author(s):  
Kunter ◽  
Kosar
Keyword(s):  
Cell Lines ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Composition Analysis ◽  
Endemic Plants ◽  
Ros Scavenging ◽  
Pharmacological Activities ◽  
Anti Cancer ◽  
Inhibitory Activities ◽  
Motility Inhibition

Recently, different herbal compounds have been studied for their curative properties against Hepatocellular Carcinoma (HCC). There are over 100 Phlomis species native to the Mediterranean region, which have pharmacological activities against gastrointestinal system and liver diseases. Phlomis brevibracteata Turrill (PBT) and Phlomis cypria Post (PCP) are endemic plants of North-Cyprus belonging to the Lamiaceae family. In this study, chemical composition of the 70% methanol extracts of Phlomis species were analyzed by LC/MS/MS and antiradical activities were evaluated by DPPH● and ABTS+● radical scavenging activity tests. Anti-carcinogenic activities on HCC cell lines were investigated using MTT and wound healing assays, intercellular ROS scavenging activities were tested by DCFH-DA assay. Forsytoside B and caffeoylquinic acids were found as the main phenolic compounds which are possibly responsible for antiradical activities of the plant extracts. Significant decrease in cell viability was noticed for both extracts at concentrations over 1000 µg/mL. PBT treatment also resulted in cell motility inhibition. Additionally, both basal and induced oxidative states of all cell lines significantly were decreased by PBT. Since extracts showed cytotoxic, antioxidant and motility inhibitory activities, Phlomis species can be a good candidate for further studies with the goal of new anticancer chemotherapeutic discoveries.

scholarly journals Population dynamics of aphids (Aphididae) on orange (Citrus sinensis ‘Thomson Navel’) and mandarin (Citrus reticulata ‘Blanco’)

2016 ◽  
Vol 107 (1) ◽  
pp. 137 ◽  
Author(s):  
Salim LEBBAL ◽  
Malik LAAMARI
Keyword(s):  
Citrus Sinensis ◽  
Common Species ◽  
Aphid Species ◽  
Climatic Conditions ◽  
Citrus Reticulata ◽  
Citrus Fruits ◽  
June July August ◽  
Aphis Spiraecola ◽  
June July ◽  
Orange Trees

Citrus fruits represent one of the most important fruit productions worldwide. However, they suffer from a numerous constraints. Aphids are among the causes of the decline in the production of citrus. In this study, the diversity of citrus aphids and their seasonal occurrence were explored on orange and mandarin, during 2012 and 2013, in an orchard located in Skikda province (Algeria). In total, six different aphid species were found during two years. The most common species was <em>Aphis spiraecola </em>Patch, 1914.  Climatic conditions had an important role in the infestation level by aphids. There were changes of aphid dynamics between the two years of the investigation. No aphids was recorded in six months in 2012 (January, June, July, August, September and December) and in three months in 2013 (January, February and August).  Besides, the number of identified aphid species increased from two to five. On the other hand, the orange trees seemed to be the most infested host species.

Molecular Docking, Antioxidant, Anticancer and Antileishmanial Effects of Newly Synthesized Quinoline Derivatives

2020 ◽  
Vol 20 (13) ◽  
pp. 1516-1529 ◽  
Author(s):  
Zoonish Malghani ◽  
Arif-Ullah Khan ◽  
Muhammad Faheem ◽  
Muhammad Z. Danish ◽  
Humaira Nadeem ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Adverse Effects ◽  
Cell Lines ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Quinoline Derivatives ◽  
Brine Shrimp Lethality Assay ◽  
Anti Cancer ◽  
Ic50 Values ◽  
Hct 116

Background: Due to the pressing need and adverse effects associated with the available anti-cancer agents, an attempt was made to develop the new anti-cancer agents with better activity and lesser adverse effects. Objective: Synthetic approaches based on chemical modification of quinoline derivatives have been undertaken with the aim of improving anti-cancer agents’ safety profile. Methods: In the present study, quinoline derivatives 6-hydroxy-2-(4-methoxyphenyl) quinoline-4-carboxylic acid (M1) and 2-(4-chlorophenyl)-6-hydroxyquinoline-4-carboxylic acid (M3) were synthesized by the reaction of aldehyde and pyruvic acid. The complete reaction was indicated by thin-layer chromatography. Newly synthesized M1and M3were tested for in silico and in vitro studies. Results: M1 and M3 were docked against selected targets. Both the test compounds showed good affinity against all targets except the p300\CBP-associated factor target as there was no H-bond formed by M1. IC50 values of M1 and M3 against 1, 1-diphenyl-picrylhydrazyl free radical scavenging activity were 562 and 136.56ng/mL, respectively. In brine shrimp lethality assay, M1 and M3 showed IC50 value of 81.98 and 139.2ng/mL, respectively. IC50 values recorded for M1 and M3 in tumor inhibition activity were 129 and 219μg/mL, respectively. M1 and M3 exhibited concentration-dependent anti-cancer effects against human cell lines of hepatocellular carcinoma (HepG2) and colon cancer (HCT-116). Against HepG2 cells, M1 and M3 exhibited IC50 of 88.6 and 43.62μg/mL, respectively. M1 and M3 utilized against HCT-116 cell lines possessed IC50 values of 62.5 and 15.3μg/mL. M1 and M3 also showed an anti-leishmanial effect with IC50 values of 336.64 and 530.142μg/mL, respectively. Conclusion: From the results of pharmacological studies, we conclude that the newly synthesized compound showed enhanced anti-oxidant, anti-cancer and anti-leishmanial profile with good yield.

scholarly journals Curcumin Encapsulated in Crosslinked Cyclodextrin Nanoparticles Enables Immediate Inhibition of Cell Growth and Efficient Killing of Cancer Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 489
Author(s):  
Karin Möller ◽  
Beth Macaulay ◽  
Thomas Bein
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Metabolism ◽  
Drug Carriers ◽  
Cell Killing ◽  
Molar Ratio ◽  
Cellular Delivery ◽  
Curcumin Nanoparticles ◽  
Anti Cancer

The efficiency of anti-cancer drugs is commonly determined by endpoint assays after extended incubation times, often after days. Here we demonstrate that curcumin encapsulated in crosslinked cyclodextrin nanoparticles (CD-NP) acts extremely rapidly on cell metabolism resulting in an immediate and complete inhibition of cell growth and in efficient cancer-cell killing only few hours after incubation. This early onset of anti-cancer action was discovered by live-cell high-throughput fluorescence microscopy using an environmental stage. To date, only very few examples of covalently crosslinked nanoscale CD-based (CD-NP) drug carriers exist. Crosslinking cyclodextrins enables the adsorption of unusually high payloads of hydrophobic curcumin (762 µg CC/mg CD-NP) reflecting a molar ratio of 2.3:1 curcumin to cyclodextrin. We have investigated the effect of CD-NP encapsulated curcumin (CD-CC-NP) in comparison to free, DMSO-derived curcumin nanoparticles (CC-NP) on 4 different cell lines. Very short incubations times as low as 1 h were applied and cell responses after medium change were subsequently followed over two days. We show that cell proliferation is inhibited nearly immediately in all cell lines and that a cell- and concentration dependent cancer-cell killing occurs. Anti-cancer effects were similar with free and encapsulated curcumin, however, encapsulation in CD-NP drastically extends the long-term photostability and anti-cancer activity of curcumin. Curcumin-sensitivity is highest in HeLa cells reaching up to 90% cell death under these conditions. Sensitivity decreased from HeLa to T24 to MDA MB-231 cells. Strikingly, the immortalized non-cancerous cell line MCF-10A was robust against curcumin concentrations that were highly toxic to the other cell lines. Our results underline the potential of curcumin as gentle and yet effective natural anti-cancer agent when delivered solvent-free in stabilizing and biocompatible drug carriers such as CD-NP that enable efficient cellular delivery.

scholarly journals Tolerance of Selected Orange and Mandarin Hybrid Fruit to Low-dose Irradiation for Quarantine Purposes

HortScience ◽  
2000 ◽  
Vol 35 (7) ◽  
pp. 1288-1291 ◽  
Author(s):  
W.R. Miller ◽  
R.E. McDonald ◽  
J. Chaparro
Keyword(s):  
Citrus Sinensis ◽  
Low Dose ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Citrus Reticulata ◽  
Citrus Fruits ◽  
Dose Irradiation ◽  
Irradiation Treatment ◽  
Dose Tolerance ◽  
Dose Dependent

Tolerance of many citrus cultivars to low-dose irradiation treatment is not known. Ten citrus cultivars grown in Florida, including the five orange [Citrus sinensis (L.) Osbeck] cultivars, Ambersweet, Hamlin, Navel, Pineapple, and Valencia, and the five mandarin hybrids (Citrus reticulata Blanco), `Fallglo', `Minneola', `Murcott', `Sunburst', and `Temple', were exposed to irradiation at 0, 0.15, 0.3, and 0.45 kGy, and stored for 14 days at 1 °C or 5 °C plus 3 days at 20 °C, to determine dose tolerance based on fruit injury. Softening of `Valencia', `Minneola', `Murcott', and `Temple' was dose-dependent, but that of other cultivars was unaffected. Only `Ambersweet', `Valencia', `Minneola', and `Murcott' did not develop peel pitting at 0.15 kGy or higher. Total soluble solids of `Ambersweet' and `Sunburst' declined slightly with increasing dose. Titratable acidity (TA) of oranges was not affected, but TA of `Sunburst' and `Temple' juice was slightly reduced by irradiation at 0.45 kGy. Juice flavor of `Hamlin', `Navel', `Valencia', and `Minneola', and pulp flavor of `Hamlin', `Valencia', `Fallglo', `Minneola', and `Murcott' was less acceptable after irradiation at 0.3 or 0.45 kGy. The appearance of all cultivars was negatively affected by the loss of glossiness with the 0.45 kGy dose. Less than 1.0% of fruit decayed and irradiation treatment had no effect on decay. Our study indicates that growers and shippers need to be aware that the effects of irradiation on citrus fruits are highly variable and both cultivar-dependent and dose-dependent.

SYNTHESIS, CHARACTERISATION, ANTIOXIDANT AND ANTICANCER EVALUATION OF NOVEL FLAVONE-4-OXIMES

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (11) ◽  
pp. 7-14
Author(s):  
B. S Jayashree ◽  
◽  
Gurushyam Sri ◽  
A Pai
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Scavenging Activity ◽  
Hep G2 ◽  
Dpph Method ◽  
Anti Cancer ◽  
Aldehyde Derivative ◽  
Cancer Activity ◽  
Better Than ◽  
Mcf 7

A series of novel flavone-4-oximes were synthesized by the oximation of substituted flavones. The synthesized compounds were characterized by various spectrochemical methods including IR, MS and NMR spectroscopy. Out of the 14 test compounds screened for their antioxidant activity, compounds such as JGS-VI (a N,N dimethyl benzaldehyde derivative) and JGS-VII (a 3,4 dimethoxy benzaldehyde derivative) exhibited antioxidant activity comparable to that of ascorbic acid and quercetin as standards following DPPH method. Compounds such as JGS-II (a p-fluoro benzaldehyde derivative), JGS-IV (a p-methyl benzaldehyde derivative) and JGS-V (a thiophene-2-aldehyde derivative) exhibited antioxidant activity among all the test compounds screened against ABTS. However, none of them showed any significant scavenging activity against nitric oxide scavenging assay in the concentration range of 200 μM-25μM. Further, anti-cancer potency for all the test compounds were evaluated by MTT assay against two different cell lines namely MCF-7 and Hep-G2. Compounds such as JGS-I (a p-chloro benzaldehyde derivative), JGS-II (a p-fluoro benzaldehyde derivative), JGS-IV, JGS-V (a thiophene -2-aldehyde derivative), JGS-VI and JGS-IX (a 3,4 chloro benzaldehyde derivative) exhibited activity better than the rest of the test compounds tested against MCF-7 cell lines. Compounds such as JGS-VI, JGS-VII, JGS-VIII (a p-bromo benzaldehyde derivative) and JGS-IX exhibited anti-cancer activity better than other test compounds tested against Hep-G2 cell lines. Thus, a few of the synthesized test compounds could become promising anti-cancer agents.

scholarly journals SKA3 Promotes Cell Growth in Breast Cancer by Inhibiting PLK-1 Protein Degradation

2020 ◽  
Vol 19 ◽  
pp. 153303382094748
Author(s):  
Li-wei Ruan ◽  
Peng-peng Li ◽  
Lang-ping Jin
Keyword(s):  
Breast Cancer ◽  
Cervical Cancer ◽  
Cell Proliferation ◽  
Cell Growth ◽  
Cell Lines ◽  
Cancer Progression ◽  
Cancer Drug ◽  
Anti Cancer ◽  
Mental And Physical Health

Breast cancer (Bca) remains the most common form of malignancy affecting females in China, leading to significant reductions in the mental and physical health of those with this condition. While spindle and kinetochore associated complex subunit 3 (SKA3) is known to be linked with cervical cancer progression, whether it is similarly associated with Bca progression remains unknown. Using shRNA, we specifically knocked down the expression of SKA3 in Bca cell lines and then assessed the resultant changes in cell proliferation using CCK-8 and colony formation assays. In addition, we used western blotting to quantify the expression levels of relevant proteins in these cells, and we assessed the interaction between SKA3 and polo-like kinase-1 (PLK-1) via co-immunoprecipitation.In this study, we observed elevated SKA3 expression in Bca tissues and cell lines. When we knocked down SKA3 expression in Bca cells, we were able to determine that it functions in an oncogenic manner so as to promote the growth and proliferation of these cells in vitro. From a mechanistic perspective, we were able to show that in Bca cells SKA functions at least in part via interacting with PLK-1 and preventing its degradation. In summary, we found that SKA3 is able to regulate PLK-1 degradation in Bca cells, thus controlling their growth and proliferation. These results highlight SKA3 as a potentially viable target for anti-cancer drug development aimed at combatting Bca.

scholarly journals Antiproliferative Properties Against Human Breast, Cervical and Ovarian Cancer Cell Lines, and Antioxidant Capacity of Leaf Aqueous Ethanolic Extract from Cotinus coggygria Scop.

2017 ◽  
Vol 44 (2) ◽  
pp. 20-25 ◽  
Author(s):  
Z. Gospodinova ◽  
N. Bózsity ◽  
M. Nikolova ◽  
M. Krasteva ◽  
I. Zupkó
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Cancer Cell Lines ◽  
Aqueous Ethanolic Extract ◽  
Cotinus Coggygria

AbstractCotinus coggygria Scop. leaf aqueous ethanolic extract was examined for its in vitro antiproliferative and antioxidant activity. Antiproliferative effect was assessed on four human gynecological cancer cell lines: breast (MCF7, T47D), cervical (HeLa) and ovarian (A2780) and compared to the cell growth inhibitory effect on non-cancerous breast epithelial cell line MCF10A using MTT cell proliferation assay. Radical scavenging assay with DPPH was applied to evaluate antioxidant potential of the extract. The obtained results showed that the herb inhibited cell growth of all of the tested cancer cell lines and the highest was the cytostatic effect on A2780 cells with a half maximal inhibitory concentration (IC50) value of 30.8 μg/ml. For the other cell lines the IC50values were in the range of 55-122.7 μg/ml. Additionally, the extract exerted considerably weaker reduction in cell proliferation of the non-cancerous cell line MCF10A compared to cancer cells, which indicates for antiproliferative selectivity. C. coggygria extract showed high free radical scavenging activity with an IC50value of 11.2 μg/ml. The obtained data provide evidence for pharmacological potential of the tested extract and future more detailed studies concerning the molecular mechanisms of the anticancer effect of the herb are needed.

scholarly journals Phenolic content and antioxidant activity of ethanolic extracts from Citrus sinensis L. and Citrus reticulata L. fruits

2020 ◽  
Vol 10 (5-s) ◽  
pp. 308-313
Author(s):  
Assia Bentahar ◽  
Amel Bouaziz ◽  
Saliha Djidel ◽  
Seddik Khennouf
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Citrus Sinensis ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Citrus Reticulata ◽  
Dry Extract ◽  
Ethanolic Extracts

Citrus fruits, in particular the genus Citrus, are very rich in antioxidants which have beneficial effects on human health. The objective of this study was to evaluate the phenolic content and the antioxidant activity of the ethanolic extracts from orange (Citrus sinensis L.) and mandarin (Citrus reticulata L.) fruits. The quantitative estimation of the total phenolic content in the two extracts revealed their richness in these compounds. The results obtained showed that the polyphenols content were 159.66 ± 2.62 mg GAE / g of dry extract and 127.33 ± 2.32 mg GAE / g of dry extract for C. sinensis and C. reticulata, respectively.The quantification of flavonoids content showed that the ethanolic extracts of C. sinensis and C. reticulata contained 0.85 ± 0.01 mg QE / g of dry extract and 0.876 ± 0.073 mg QE / g of dry extract, respectively. Whereas, the tannins contents were 46.32 ± 1.02 mg TAE / g of dry extract for  C. sinensis and 47.65 ± 1.36 mg TAE / g of dry extract for C. reticulata. In vitro antioxidant activity was evaluated using two tests: the reducing power and the hydroxyl radical scavenger test. The evaluation of the two extracts by the ferric iron reducing power test showed an EC50 value of 0.882 ± 0.037 mg / ml for  C. sinensis extract and an EC50 of 1.085 ± 0.068 mg / ml for C. reticulata extract. In addition, C. sinensis and C. reticulata showed good hydroxyl radical scavenging effect with IC50 values of 0.303 ± 0.026 mg /ml and 0.572 ± 0.100 mg / ml for C. sinensis and C. reticulata, respectively. These results suggested that these fruit extracts could be good sources of phenolic compounds and ingredients with high antioxidant potential. Keywords: Citrus sinensis, Citrus reticulata, Polyphenols, Flavonoids, Tannins, Antioxidant activity.

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