Genetic analysis of the hybrid rice population ‘Mavr × Kontakt’

Rice can form not only white-color kernels, but also of red, brown and black colors. In black-color rice kernels, the pericarp contains anthocyanin, which has antioxidant activity and therefore has a positive effect on human health. The purpose of the current study was to develop rice samples with black pericarp. The paper has presented the study results of the hybrid population ‘Mavr × Kontakt’ of the second generation. The variety ‘Mavr’ has a black pericarp, the variety ‘Kontakt’ has a white one. In the process of hybridological analysis there have been identified the patterns of inheritance of the main quantitative traits affecting productivity; there have been identified the best samples, in which formed compact erect panicles and black kernels; there has been selected the initial material for breeding work. The study was carried out in 2020 on the plots of the ES “Proletarskoye” in the Rostov region. There was established that the color of pericarp was inherited according to the type of complementary interaction of two genes. There was found an overdominance and interaction of two pairs of genes of different strengths according to ‘plant height’. There was also seen partial positive dominance, transgressions, and digenic differences of the initial varieties according to ‘panicle length’. According to the traits ‘number of spikelets per panicle’, there was identified overdominance of large values and positive transgression. There were found the forms with well-kerneled panicles. The trait ‘1000 kernel weight’ was characterized by negative dominance and dihybrid cleavage of 9:6:1. There have been selected the best morphotype F2 forms with black pericarp, which possessed optimal plant height, long panicles, larger kernel size, and an average 1000 kernel weight.

Breeding and genetic peculiarities of spring barley 1000 kernel weight under conditions of the central part of the Ukrainian Forest-Steppe

Purpose. To reveal the breeding and genetic peculiarities for spring barley 1000 kernel weight and to identify genetic sources for breeding under conditions of the central part of the Forest-Steppe of Ukraine. Methods. Investigations were carried out at the V. M. Remeslo Myronivka Institute of Wheat of the NAAS of Ukraine. Spring barley F1 of two comp­lete (6 ´ 6) diallel crossing schemes was investigated. The first scheme included modern both domestic and foreign spring barley malting varieties (‘MIP Tytul’, ‘Avhur’, ‘Datcha’, ‘Quench’, ‘Gladys’, ‘Beatrix’), the second – classic covered awned (‘MIP Myroslav’, ‘Sebastian’), awnless (‘Kozyr’, ‘Vitrazh’) and naked (‘Condor’, ‘CDC Rattan’) varieties. Results. Various types of inheritance of 1000 kernel weight were revealed, with the exception of negative dominance. The proportion of combinations with corresponding values ​​of the degree of phenotypic dominance varied depending on the genotypes involved in the crossing and the years of testing. In both crossing schemes, both in 2019 and in 2020, positive overdominance was found in most combinations. The grea­test number of combinations with the manifestation of hete­rosis under different growing conditions was noted when varieties ‘Gladys’ and ‘MIP Myroslav’ were used in crossing. According to the parameters of genetic variation, it was revealed that the trait was determined mainly by the additive-dominant system. The dominance was aimed at increasing the 1000 kernel weight. Only in the second crossing scheme in 2019 the value of the directional dominance indicator was unreliable. In the loci, incomplete dominance in 2019 and over-dominance in 2020 were revealed. Reliably high effects of the general combining ability in both years were noted for varieties ‘Datcha’, ‘Gladys’, ‘MIP Myroslav’, ‘Kozyr’ and ‘Vitrazh’. Conclusions. The revealed breeding and genetic peculiarities indicate that for the overwhelming majority of created hybrid combinations will be required the final selection in later generations, when the dominant alleles are homozygous. Spring barley awned varieties ‘Gladys’, ‘MIP Myroslav’ and ‘Datcha’, as well as awnless varieties ‘Kozyr’ and ‘Vitrage’ can be used as effective genetic sources for increa­sing the 1000 kernel weight

Comparison of the F2 and F3 traits’ inheritance by the rice hybrid ‘Kontro x Kuboyar’

Rice is a very moisture-loving crop, therefore it suffers greatly from drought. However, in the world in recent years, there is an increasing shortage of fresh water, which makes the use of upland rice relevant. Therefore, rice varieties with a reduced demand for water supply, developed as a result of breeding work, are more profitable for agricultural producers than agrotechnical or reclamation measures. It is much cheaper to produce such rice with periodic irrigation. The purpose of the current study was to analyze genetically the main quantitative traits of the rice hybrid ‘Kontro x Kuboyar’ with the selection of the best forms for subsequent breeding for drought resistance. The study was carried out in 2019–2020 on the rice plots of the special subdivision ‘Proletarskoye’ belonging to of the Agricultural Research Center “Donskoy” in the Rostov Region. In the course of the study there has been made a genetic analysis of a number of quantitative traits that determine rice productivity. The character of traits’ inheritance in the second and third generations was identical. The inheritance of the trait ‘panicle length’ was characterized by partial negative dominance and monogenic differences in parental varieties. The trait ‘number of spikelets per panicle’ was inherited according to the type of negative dominance of smaller values of the trait and was determined by the interaction of two pairs of genes. The trait ‘1000-kernel weight’ was identified by the partial dominance of large values of the trait and monogenic differences in the parental varieties. The traits ‘kernel length’ and ‘kernel width’ were split according to the monohybrid scheme 1:2:1, and there was no dominance. From the third generation, the best early-maturing forms with optimal values of the traits ‘plant height’, ‘panicle length’, ‘1000-kernel weight’ and ‘the best kernel percentage per panicle’ were selected for the subsequent breeding of drought-resistant rice varieties.

Human STAT3 variants underlie autosomal dominant hyper-IgE syndrome by negative dominance

Most patients with autosomal dominant hyper-IgE syndrome (AD-HIES) carry rare heterozygous STAT3 variants. Only six of the 135 in-frame variants reported have been experimentally shown to be dominant negative (DN), and it has been recently suggested that eight out-of-frame variants operate by haploinsufficiency. We experimentally tested these 143 variants, 7 novel out-of-frame variants found in HIES patients, and other STAT3 variants from the general population. Strikingly, all 15 out-of-frame variants were DN via their encoded (1) truncated proteins, (2) neoproteins generated from a translation reinitiation codon, and (3) isoforms from alternative transcripts or a combination thereof. Moreover, 128 of the 135 in-frame variants (95%) were also DN. The patients carrying the seven non-DN STAT3 in-frame variants have not been studied for other genetic etiologies. Finally, none of the variants from the general population tested, including an out-of-frame variant, were DN. Overall, our findings show that heterozygous STAT3 variants, whether in or out of frame, underlie AD-HIES through negative dominance rather than haploinsufficiency.

Peculiarities of the phenotypic dominance degree manifestation by stem length in F1bread winter wheat

The manifestation of the phenotypic dominance degree of the main stem length in F1 under hybridization of different varieties of bread winter wheat was studied during different for hydrothermal conditions 2018–2020. In the hybridization of Bilotserkivska napivkarlykova variety with medium-growing varieties of groups 1 and 2, the most common type of stem length inheritance was negative overdominance – 54.5 % and positive overdominance – 27.3 %. The degree of phenotypic dominance varied from -63.0 to +7.4. When used medium-sized varieties of group I in the crossing with the maternal form, the degree of phenotypic dominance had a signifcant differentiation from -257.0 to +35.0, and the determination of stem length in most combinations was negatively overdominated – 61.4 %. The combinations Shchedra nyva/Dobirna, Shchedra nyva/Vidrada, Myronivska early/Kolchuga, Antonivka/Vidrada were characterized by a stable manifestation of a negative degree of phenotypic dominance (h p > -1). When involved in the hybridization of medium-sized varieties of group 2 in the maternal form, the most common type of stem length inheritance had negative dominance as well. It was found that the degree of phenotypic dominance of stem length in F 1 depended on the selection of parental forms for hybridization and year conditions. Thus, in 2020, under more favorable conditions for the formation of stem length in most hybrids, a negative degree of phenotypic dominance was determined, and the inheritance was negatively dominated. High rates of negative hypothetical and true heterosis were found in the following hybrids: Shchedra nyva/Dobirna, Shchedra nyva/Vidrada, Kolchuga/Chornyava, Kolchuga/ Antonivka, Yednist/Dobirna, Kolchuga/Vidrada in 2018; Chornyava/Antonivka, Shchedra nyva/Dobirna, Shchedra nyva/Vidrada, Chorniava/Shchedra nyva in 2019; Myronivska early/Antonivka, Zolotokolosa/Antonivka, Myronivska early/Kolchuga, Myronivska early/Vdala, Zolotokolosa/ Yednist, Shchedra nyva/Stolychna, Shchedra nyva/Vidrada, Antonivka/Yednist, Antonivka/Vidrada, Kolchuga/Vidrada, Kolchuga/Stolychna in 2020. Key words: bread winter wheat, crossbreeding combinations, hybrids, main stem length, degree of phenotypic dominance, hypothetical and true heterosis.

A Multi-platform Approach to Monitoring Negative Dominance for COVID-19 Vaccine-Related Information Online

Objective The aim of this study was to test the appearance of negative dominance in COVID-19 vaccine-related information and activity online. We hypothesized that if negative dominance appeared, it would be a reflection of peaks in adverse events related to the vaccine, that negative content would attract more engagement on social media than other vaccine-related posts, and posts referencing adverse events related to COVID-19 vaccination would have a higher average toxicity score. Methods We collected data using Google Trends for search behavior, CrowdTangle for social media data, and Media Cloud for media stories, and compared them against the dates of key adverse events related to COVID-19. We used Communalytic to analyze the toxicity of social media posts by platform and topic. Results While our first hypothesis was partially supported, with peaks in search behavior for image and YouTube videos driven by adverse events, we did not find negative dominance in other types of searches or patterns of attention by news media or on social media. Conclusion We did not find evidence in our data to prove the negative dominance of adverse events related to COVID-19 vaccination on social media. Future studies should corroborate these findings and, if consistent, focus on explaining why this may be the case.

Characteristics of the Ugandan rice varieties grown in the Rostov region

Rice is a valuable agricultural crop that is used as food for more than half of the world's population. The development of highly productive varieties and hybrids with high product quality assumes the utilization of new initial material from different countries in the breeding process. The purpose of the current research was to conduct an ecological testing of the rice varieties from Uganda in the Proletarsky district of the Rostov region in Russia and use them in the breeding process. There were studied the Ugandan rice samples ‘Nerica 1’, ‘Nerica 4’, ‘Komboka’, ‘Wita 9’, ‘Namche 1’, ‘Namche 2’, ‘Namche 3’, ‘Namche 4’, ‘Namche 5’, ‘Namche 6’ (NaCRRI) in comparison with the Russian variety ‘Komandor’ (FSBSI “ARC “Donskoy”). The study of the Ugandan rice varieties according to a number of economically valuable traits and biological properties showed that the vegetation period from soil flooding to rice maturing took 100–113 days, the standard variety ‘Komandor’ needed 83 days. The varieties ‘Komboka’ and ‘Wita 9’ did not bear blossom. All samples had a short habit, with 63-95 cm of plant height. The long drooping panicles of 18.5–22.3 cm carried from 122 to 213 seeds. The caryopses were elongated, the length of the caryopses varied from 9.0 to 9.5 mm, and the width ranged from 2.7 to 3.1 mm. 1000 seed weight varied from 26 to 34 g, number of seeds per panicle ranged from 122 to 213 pieces. Genetic analysis of the second generation hybrids ‘Kontakt × Nerica 1’ and ‘Boyarin × Nerica 4’ showed different types of traits’ inheritance, from negative dominance (vegetation period) through the absence of dominance (caryopses size) to overdominance (plant height, seed weight per panicle and number of seeds per panicle).

Expanding the Nude SCID/CID Phenotype Associated with FOXN1 Homozygous, Compound Heterozygous, or Heterozygous Mutations

Human nude SCID is a rare autosomal recessive inborn error of immunity (IEI) characterized by congenital athymia, alopecia, and nail dystrophy. Few cases have been reported to date. However, the recent introduction of newborn screening for IEIs and high-throughput sequencing has led to the identification of novel and atypical cases. Moreover, immunological alterations have been recently described in patients carrying heterozygous mutations. The aim of this paper is to describe the extended phenotype associated with FOXN1 homozygous, compound heterozygous, or heterozygous mutations. We collected clinical and laboratory information of a cohort of 11 homozygous, 2 compound heterozygous, and 5 heterozygous patients with recurrent severe infections. All, except one heterozygous patient, had signs of CID or SCID. Nail dystrophy and alopecia, that represent the hallmarks of the syndrome, were not always present, while almost 50% of the patients developed Omenn syndrome. One patient with hypomorphic compound heterozygous mutations had a late-onset atypical phenotype. A SCID-like phenotype was observed in 4 heterozygous patients coming from the same family. A spectrum of clinical manifestations may be associated with different mutations. The severity of the clinical phenotype likely depends on the amount of residual activity of the gene product, as previously observed for other SCID-related genes. The severity of the manifestations in this heterozygous family may suggest a mechanism of negative dominance of the specific mutation or the presence of additional mutations in noncoding regions.

Analysis of the inheritance of quantitative traits in the rice hybrid Kuboyar × Gagat

Rice is one of the main food items in the world. White rice is mainly used, but there are also varieties with red, brown and black pericarp grains. This rice is much healthier. The article is devoted to the creation of new lines of rice with black pericarp. The purpose of the work is to study the inheritance of varying quantitative traits in an interspecific hybrid of rice Kuboyar × Gagat, with subsequent selection of isolated samples. Methods. Hybridization of these varieties was carried out in 2017. Mathematical processing of research data was performed Using the program Statistica 6. For genetic analysis, the program “Polygen A” was used by A. F. Merezhko (2005). The research was conducted in 2018–2019 on the basis of a Separate division “Proletarskoe” of the Rostov region. Scientific novelty. A genetic analysis of varying quantitative traits that affect the grain productivity of rice was performed, and a number of new patterns were established. Results. Inheritance of plant height in F2 hybrids was based on the type of overdomination of large trait values. The parent forms differed in the allelic state of the two pairs of genes. Along the length of the panicle, there was a partial negative dominance and monogenic differences in crossed varieties. According to the number of spikelets on the panicle, overdomination of a larger trait value and the interaction of two pairs of genes were established. By the mass of 1000 grains, partial dominance of large values of the trait and monogenic differences in the initial parent forms were established. There was no dominance in the length of the grain, and the parent forms differed by two pairs of genes. The grain width was dominated by smaller values of the trait, splitting was performed according to the monohybrid scheme. The selection of isolated samples forming a compact erect panicle and grains with a black pericarp was carried out for further selection work.