The Isoenzymic Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453—An Episodic History and Still Mysterious after 60 Years

Researching the involvement of molecular oxygen in the degradation of the naturally occurring bicyclic terpene camphor has generated a six-decade history of fascinating monooxygenase biochemistry. While an extensive bibliography exists reporting the many varied studies on camphor 5-monooxygenase, the initiating enzyme of the relevant catabolic pathway in Pseudomonas putida ATCC 17453, the equivalent recorded history of the isoenzymic diketocamphane monooxygenases, the enzymes that facilitate the initial ring cleavage of the bicyclic terpene, is both less extensive and more enigmatic. First referred to as ‘ketolactonase—an enzyme for cyclic lactonization’—the enzyme now classified as 2,5-diketocamphane 1,2-monooxygenase (EC 1.14.14.108) holds a special place in the history of oxygen-dependent biochemistry, being the first biocatalyst confirmed to undertake a biooxygenation reaction equivalent to the peracid-catalysed Baeyer–Villiger chemical oxidation first reported in the late 19th century. However, following that auspicious beginning, the biochemistry of EC 1.14.14.108, and its isoenzymic partner 3,6-diketocamphane 1,6-monooxygenase (EC 1.14.14.155) was dogged for many years by the mistaken belief that the enzymes were true flavoproteins that function with a tightly-bound flavin cofactor in the active site. This misconception led to a number of erroneous interpretations of relevant experimental data. It is only in the last decade, initially as the result of pure serendipity, that these enzymes have been confirmed to be members of a relatively recently discovered class of oxygen-dependent enzymes, the flavin-dependent two-component monooxygenases. This has promoted a renaissance of interest in the enzymes, resulting in programmes of research that have significantly expanded current knowledge of both their mode of action and regulation in camphor-grown P. putida ATCC 17453. However, some features of the biochemistry of the isoenzymic diketocamphane monooxygenases remain currently unexplained. It is the episodic history of these enzymes and some of what remains unresolved that are the principal subjects of this review.

Constraints and Prospects of Mechanized Processing of Forest Tree Seeds

Trees are generally propagated from seeds and the suitability and quality of the seeds have a big effect on the success of the plantations, afforestation and reforestation programmes established from their seedlings. In view of the fact that good forestry projects start with good seeds, the quality as well as quantity of tree seeds is very important. Processing of seeds is one of the main factors that determined the quality and quantity of seeds. However, processing of forest tree seeds in Nigeria and other developing countries has mostly been carried out using traditional methods which affects the quality of the seeds especially for large quantity of seeds. This work attempts to raise awareness on the need to mechanize processing of forest tree seeds so as to increase the quantity and quality of the seeds and to reduce the drudgery, time and health hazards involved in the common traditional methods of processing the seeds. Tree seeds are often regarded as inferior to crop seeds, a misnomer (mistaken belief) that needs to be rectified to promote tree planting.

Towards a computational theory of social groups: A finite set of cognitive primitives for representing any and all social groups in the context of conflict

We don't yet have adequate theories of what the human mind is representing when it represents a social group. Worse still, many people think we do. This mistaken belief is a consequence of the state of play: Until now, researchers have relied on their own intuitions to link up the concept social group on the one hand, and the results of particular studies or models on the other. While necessary, this reliance on intuition has been purchased at considerable cost. When looked at soberly, existing theories of social groups are either (i) literal, but not remotely adequate (such as models built atop economic games), or (ii) simply metaphorical (typically a subsumption or containment metaphor). Intuition is filling in the gaps of an explicit theory. This paper presents a computational theory of what, literally, a group representation is in the context of conflict: it is the assignment of agents to specific roles within a small number of triadic interaction types. This “mental definition” of a group paves the way for a computational theory of social groups—in that it provides a theory of what exactly the information-processing problem of representing and reasoning about a group is. For psychologists, this paper offers a different way to conceptualize and study groups, and suggests that a non-tautological definition of a social group is possible. For cognitive scientists, this paper provides a computational benchmark against which natural and artificial intelligences can be held.

When Learners Prefer the Wrong Explanation: Misconceptions in Physics Explainer Videos and the Illusion of Understanding

Online explanation videos on platforms like YouTube are popular among students and serve as an important resource for both distance learning and regular science education. Despite their immense potential, some of the explainer videos for physics include problematic explanation approaches, possibly fostering misconceptions. However, some of them manage to achieve good ratings on YouTube. A possible reason could be that explainer videos with misconceptions foster an “illusion of understanding”—the mistaken belief that a topic has been understood. In particular, misconceptions close to everyday experiences might elicit greater interest and appear more convincing than scientifically correct explanations. This experimental study was conducted to research this effect. Physics learners (N = 149), with a low prior knowledge enrolled in introductory university courses on primary education, were randomly assigned to experimental and control groups. While the experimental group watched a video introducing the concept of force relying on misconceptions, the control group watched the scientifically correct video. Both videos were comparable in terms of comprehensibility and duration. In the posttest, the experimental group believed that the video was scientifically correct, well-explained, and that they do not require further instruction to understand the concept—indicators of an illusion of understanding. The video including misconceptions was perceived as better understandable than the scientifically correct video (d = 0.62*). The experimental group was significantly more convinced by the misconception after watching the video than the control group (d = 1.86**). They learnt more erroneous knowledge about the misconception than the control group about the scientifically correct concept (Cohen’s q = 0.37*). We argue that this might become problematic (a) in physics instruction because students who have watched a misleading video might regard further teaching in school as irrelevant, and (b) learners might tend to rate videos including misconceptions better on an online platform like YouTube.

Debates on the Peasant Question in the Nikolaevan Era

A large scholarly literature exists about plans for a peasant reform in the reign of Nicholas I. However, the most important archival documents about the debates on the peasant question remain unpublished. The new book by T. V. Andreeva “The distant approaches to the Great Reform: The peasant question in Russia in the reign of Nicholas I” seeks to fill this lacuna. The book begins with a historical survey of the six government committees that were tasked with planning reforms, followed by an extensive collection of archival documents of both official and private provenance. In the debates under Nicholas I, the specific problem of serfdom was folded into the larger question of the social position of the peasants, which the government regarded as a source of both political instability and economic backwardness. The solution that officials envisioned was a reform that was comprehensive, multi-faceted, and gradual. Step-by-step, the evolution that had led to creation of serfdom from the 17th century onward was to be reversed: the landlords were gradually to lose their power over the person of the serfs, who were to be attached only to the land itself. Eventually, the serfs were to be emancipated with land; in the meantime, restrictions on the power of landlords and a comprehensive reform of the state peasants were to serve as preparatory steps. According to Andreeva, the vision of Nicholas and his advisors was too limited and conservative, and premised on the mistaken belief that it was possible to modernize the country without touching the core of the sociopolitical system.

9. Defences of compulsion

This chapter focuses on legal defences to criminal offences in Great Britain that will result in acquittal, which include duress and duress of circumstances, necessity, compulsion, public and private defence, and mistaken belief. These defences can be divided into justifications and excuses, and most of them consist of subjective and objective elements. The chapter explains the general principles of these excusatory and justificatory defences, and evaluates proposed reforms of criminal law covering these types of defence. It also provides examples of relevant cases and analyses the bases of court decisions in each of them.

Understanding Police Interrogation

What drives suspects to confess during police interrogation? In particular, why do some people falsely confess to serious crimes, despite both the likelihood of severe negative consequences and their actual innocence? Too often, observers endorse the mistaken belief that only people with severe mental illnesses or cognitive disabilities would confess falsely. This common but erroneous belief overlooks the risks that result from additional factors that can influence the nature of an interrogation and may conduce to a false confession, including investigators’ biases, cultural views about race and crime, the powerful effects of police deception on suspects, and characteristics of the suspect and of the circumstances that can increase the suspect’s vulnerability. This book examines numerous cases of false confession to clarify the totality of the circumstances surrounding interrogation and confession, including the interactions of many psychological, legal, cultural, personal, and other factors that lead to greater likelihood of confessions, including coerced or false confessions. It presents recommendations for reforming police interrogation in order to produce accurate, detailed confessions from factually guilty suspects, confessions that stand up under rigorous legal review, are admissible at trial, and lead to guilty verdicts.

Unexceptionalism

This chapter highlights another habit of thought that compromises one's ability to think clearly about strategies for governing in the United States: the doctrine of exceptionalism—the idea that there is something unique about governance in the American context. This mistaken belief makes it harder to learn from the experience of other states. There is a temptation to suggest that the United States is exceptional only because of the emphasis that Americans put on their own exceptionality. But even this is not true. People in most countries see something special about their own circumstances. Indeed, the feeling of exceptionality is shared almost universally, and rightly so. Conditions facing leaders in any one state are different from those facing any other state. Governance strategies must fit those conditions, and consequently one should expect policies and institutions to vary as well. This is why one should be wary about “one size fits all” prescriptions for governmental reform. Still, one should not get too carried away with this emphasis on variation. Even if circumstances change, there is one important commonality: leaders in all states, at all points in time, deal with the realities of building and maintaining a state.

One More Twist ~ Knowledge How and Ability

According to Bengson et al.’s (2009) Salchow case, Irina is a novice skater who (1) has a mistaken belief about what amounts to a Salchow, but also (2) has a neurological abnormality which, unknowingly to her, affects both her movement and her sense of it. As a result of this twist, she (3) always ends up succeeding in jumping the Salchow whenever she tries. This story was presented as a counterexample to a variant of anti-intellectualism, and as Bengson and colleagues expected, the vast majority of participants in their survey judged both that Irina is able to do the Salchow and that she does not know how to do it. But the three conditions above leave some ambiguity about the story. That is whether Irina is aware of her own ability, or whether she is aware of what she is doing when she performs it, and therefore the fact that she can reliably perform the Salchow. However, as we report here, disambiguating this point will radically change the responses of people, which rather poses a serious challenge to intellectualism.

Simultaneous Underweighting and Overestimation of Rare Events: Unpacking a Paradox

We investigated previous findings suggesting a paradoxical inconsistency of people’s beliefs and choices: When making decisions under uncertainty, people seem to both overestimate the probability of rare events in their judgments and underweight the probability of the same rare events in their choices. In our re-examination, we found that people’s beliefs are consistent with their decisions, but they do not necessarily correspond with the environment. Both overestimation and underweighting of the rare event seemed to result from (most, but not all) participants’ mistaken belief that they can infer and exploit sequential patterns in a static environment. In addition, we found that such inaccurate representations can be improved through incentives. Finally, detailed analysis suggested a mixture of individual level response patterns, which can give rise to an erroneous interpretation of group-level patterns. Our results offer an explanation for why beliefs and decisions can appear contradictory and present challenges to some current models of decisions under uncertainty.