Conventional histological and cytological staining with simultaneous immunohistochemistry enabled by invisible chromogens

Conventional histological stains, such as hematoxylin plus eosin (H&E), and immunohistochemistry (IHC) are mainstays of histology that provide complementary diagnostic information. H&E and IHC currently require separate slides, because the stains would otherwise obscure one another. This consumes small specimen, limiting the total amount of testing. Additionally, performing H&E and IHC on different slides does not permit comparison of staining at the single cell level, since the same cells are not present on each slide, and alignment of tissue features can be problematic due to changes in tissue landscape with sectioning. We have solved these problems by performing conventional staining and IHC on the same slide using invisible IHC chromogens, such that the chromogens are not visible when viewing the conventional stain and the conventional stain is excluded from images of the IHC. Covalently deposited chromogens provided a convenient route to invisible chromogen design and are stable to reagents used in conventional staining. A dual-camera brightfield microscope system was developed that permits simultaneous viewing of both visible conventional stains and invisible IHC chromogens. Simultaneous staining was demonstrated on several formalin-fixed paraffin-embedded tissue specimens using single and duplex IHC, with chromogens that absorb ultraviolet and near infrared light, followed by H&E staining. The concept was extended to other conventional stains, including mucicarmine special stain and Papanicoulou stain, and further extended to cytology specimens. In addition to interactive video review, images were recorded using multispectral imaging and image processing to provide flexible production of color composite images and enable quantitative analysis.

Mineral Exploration in Mawat Region, Kurdistan-Iraq, Based on Satellite Data and Terrain Prospection

The main goal of the presented exploration was to estimate potential for mineralization in the Mawat ophiolitic massif in Kurdistan, Iraq. The aim of the study was to explore existing copper mineralization and assessor elements gold, platinoids and chromium. Geological exploration detected two types of Cu occurrence a) secondary Cu carbonates (malachite) and b) Cu sulfides (chalcopyrite-pyrite). The Mawat region is mostly built of ultrabasic and basic rocks: peridotites, gabbros, serpentinites and basalts which are heavily deformed, with faults mostly oriented NNW-SSE, and NE-SW. The first phase of exploration comprised digital processing of ASTER and QuickBird satellite images, with appropriate geometrical and radiometric corrections and transformation into coordinate system. Color composite images were produced in different scales. They served to define lithological composition, tectonic settings, location of the points of interest etc. The field work was designed to check satellite data in situ, with focus on perspective rock formations, which might host copper mineralization, and other elements. The host rocks of the ore occurrences are primarily gabbros and metagabbros intersected by diabase dykes, epidote and quartz veins. Secondary mineralization is the product of surficial weathering and it is represented by malachite and limonite. The geophysical survey was very useful in the detection of area with elevated induced polarization and low resistivity. Three perspective areas have been selected for detailed explorations: Waraz, Mirava-Chenara and Konjirin-Kuradawi. The concentration of copper varies highly in very wide ranges; the maximum measured concentration of Cu was determined in Waraz area 6.7%. Some rock samples also show concentration of gold from 0.36 to 2.59 ppm Au. Keywords: Mawat ophiolitic massif, geologic-geophysical explorations, copper mineralization, Kurdistan-Iraq

Coral reef monitoring in Panjang Island, Central Bangka

Bangka Island is the largest tin producer in Indonesia and since the granting of tin mining freedom in 2000, unconventional tin mining (TI) is increasingly prevalent. The existence of mining activities will directly or indirectly damage the environment both on land and at sea. Especially the high biodiversity of coral reef ecosystem. The purpose of this research was to analyze a map of the distribution of coral reef based on Sentinel 2A satellite imagery data. Analyze the extent of the coral reefs in shallow waters of Putri Island, and analyze of the condition coral reefs (percentage cover, mortality index and genus diversity) with using collaboration betwen the coral diving data and remote sensing data. Studies of changes in coral reef ecosystems have been ongoing since several decades ago. The combination of satellite imagery and aerial photographs is capable of making long-term and continuous observations on mapping and change detection. Remote sensing technology has several advantages overconventional sampling to monitor a large area in time almost simultaneously and continuously including the difficult to explore areas. This research was conducted with visual interpretation by using standard true color composite band (483) and false color composite band (843) of Sentinel 2A and also using lyzenga transformation. Estimation of coral reefs area based on result is 475,96 ha (2016) and decreased to 475 ha (2021). The condition of coral reefs at the research location is a good condition.

Interpretation of the Geological Romance of Kendari Bay Using Landsat 5 TM

Kendari Bay is part of Kendari City. Kendari Bay is a city landmark in addition to its economic and environmental functions.. The condition of Kendari Bay is experiencing a fairly high sedimentation problem, so that it begins to cause problems with land availability and space utilization patterns in the Kendari Bay area. The endogenous process of dynamic earth activities is a part that needs to be considered in managing that problems. This study aims the geological conditions of Kendari Bay by using remote sensing as a form of studying the geological conditions of Kendari Bay. Using Landsat 5 TM imagery in 1990, it was used to see the natural geological conditions of Kendari Bay, using the false color composite method and interpretation of photo images visually and validated through field observations. Landsat image processing and interpretation using Quantum GIS, resulted in Kendari bay can be divided into several physiography, namely coastal zone, estuary zone, fluvial zone, plain zone, hill zone, and mountain zone (Head land). The physiography can be seen from the geological characteristics such as the lithology which is composed of alluvial deposits to limestone. The results of interpretation of composite color images and validation of direct observation data in the field show a qualitative suitability.

Geospatial Interpretation of Onshore Hydrocarbon Micro–Seepage Induced Alterations in Soils and Sediments by Spectral Enhancement Techniques

Hydrocarbon micro – seepages are light hydrocarbon that cause oxidation – reduction reaction on the earth’s surface, resulting in alterations and anomalies such as red bed bleaching, ferrous iron enrichment and increase in the concentration of clay minerals and carbonate in overlying soils and sediments. Remote sensing has become a valuable tool in hydrocarbon micro – seepage studies and have been successfully used to interpret surface alterations and anomalies of minerals. In this study, Landsat 7 ETM+ remotely sensed data was utilized for interpreting the onshore hydrocarbon micro – seepage induced alterations zone in Ugwueme. Spectral enhancements techniques such as the principal component analysis (PCA), band ratio (BR) and false color composite (FCC) were adopted for delineating alteration zones. With Landsat 7 ETM+ band selection, and for PCA, the 1457PC3, 1345PC2 and 3457PC4 are the most suitable PC image for spectral enhancement of ferric iron, ferrous iron and clay minerals. Band ratio index such as (3/1), (7/5) and (2+5)/(3+4) also yields better enhancement for anomalous micro – seepage. The study shows that PCA, BR, FCC are good spectral enhancement techniques for interpreting hydrocarbon micro – seepage alterations in overlying soils and sediments.

Multisource Data Analysis for Gold Potentiality Mapping of Atalla Area and Its Environs, Central Eastern Desert, Egypt

In this research, airborne geophysical and remote sensing datasets were integrated for gold potentiality mapping (GPM) over the Atalla area in Central Eastern Desert, Egypt. Utilizing aeromagnetic data, detailed structural complexity maps were constructed using the center for exploration targeting (CET) procedure. Then, spectrometric gamma-ray data primarily located hydrothermally altered tracts with discriminating various rock units. The latter are precisely outlined by implementing various techniques (false-color composite (FCC), band ratio (BR), relative absorption band depth (RBD), directed principal component analysis (DPCA), and constrained energy minimization (CEM)) to ASTER, Sentinel 2 and ALOS PRISM datasets, with reference to the geological maps. The study exhibits that gold mineralization is structurally controlled by NW-SE direction. The findings of structural complexity and hydrothermal alteration (argillic, advanced argillic, phyllic, and propylitic) were used as weighted inputs for contouring gold potentiality. The resultant GPM accentuated five gold-promising zones; two are confirmed via locations of ancient gold mines, while the remaining three zones are strongly recommended for their gold potentiality.

Paradigm of Geological Mapping of the Adıyaman Fault Zone of Eastern Turkey Using Landsat 8 Remotely Sensed Data Coupled with PCA, ICA, and MNFA Techniques

A principal and independent component analysis (PCA and ICA) and a minimum noise fraction analysis (MNFA) were applied in this study to Landsat 8 Operational Land Imager (OLI) images along the Adıyaman fault zone in Eastern Turkey. These analyses indicated that the lithologic units, fault patterns, and the morphological and structural features can be mapped highly accurately by using spectral-matching techniques in regions where rocks are well exposed. An inspection of all possible band combinations indicated that the PCA 134 and 231 and the ICA 132 band combinations give the best false color composite images for identifying the lithological units and contacts. The findings of the MNFA band combinations show that the MNFA 521 band combination also is robust for discriminating the lithological units, particularly Quaternary clastic units (colluvium/alluvium). MNFA band 1 alone provides the best image for tracing the tectonic and structural elements in the study area. The new up-to-date lithologic map of the Adıyaman fault zone we produced upon the interpretation of the processed OLI images reveals several river channels that are offset and beheaded by the Adıyaman fault, which verifies its Quaternary activity. This study demonstrated that, when used with the OLI data, the PCA, ICA, and MNFA are very powerful for lithological and structural mapping in actively deforming tectonic zones and hence can be applied to other regions elsewhere in the world where the climate is arid to semiarid, and the vegetation cover is scarce.

A Single-Shot 3D Measuring Method Based on Quadrature Phase-Shifting Color Composite Grating Projection

A single-shot three-dimensional measuring method based on quadrature phase-shifting color composite grating projection is proposed. Firstly, three quadrature phase-shifting sinusoidal gratings are encoded in red (R), green (G), and blue (B) channels respectively, composed single- frame color composite grating. This color composite grating is projecting obliquely on the object by DLP. After that, the color camera which is placed in a specific location is used to capture the corresponding color deformed pattern and send it to the PC. Then, by color separation, the color deformed pattern is demodulated as the corresponding three-frame monochromatic deformed patterns with a shifted quadrature phase. Due to the existences of sensitivity differences and color crosstalk among the tricolor channels, we propose a gray imbalance correction method based on the DC component’s consistency approximation. By the established 3D reconstruction physical model, the measurement of 3D shape can be achieved. Many experimental results for static and moving objects prove the proposed method’s feasibility and practicability. Owing to the single-shot feature of the proposed method, it has a good application prospect in real-time and high-speed 3D measurement.

Application of optical and radar data for lineaments mapping in Kerdous inlier of the Anti Atlas belt, Morocco

<p>Lineament mapping is an important step for lithological and hydrothermal alterations mapping. It is considered as an efficient research task which can be a part of structural investigation and mineral ore deposits identification. The availability of optical as well as radar remote sensing data, such as Landsat 8 OLI, Terra ASTER and ALOS PALSAR data, allows lineaments mapping at regional and national scale. The accuracy of the obtained results depends strongly on the spatial and spectral resolution of the data. The aim of this study was to compare Landsat 8 OLI, Terra ASTER, and radar ALOS PALSAR satellite data for automatic and manual lineaments extraction. The module Line of PCI Geomatica software was applied on PC1 OLI, PC3 ASTER and HH and HV polarization images to automatically extract geological lineaments. However, the manual extraction was achieved using the RGB color composite of the directional filtered images N - S (0°), NE - SW (45°) and E - W (90°) of the OLI panchromatic band 8. The obtained lineaments from automatic and manual extraction were compared against the faults and photo-geological lineaments digitized from the existing geological map of the study area. The extracted lineaments from PC1 OLI and ALOS PALSAR polarizations images showed the best correlation with faults and photo-geological lineaments. The results indicate that the lineaments extracted from HH and HV polarizations of ALOS PALSAR radar data used in this study, with 1499 and 1507 extracted lineaments, were more efficient for structural lineament mapping, as well as the PC1 OLI image with 1057 lineaments.</p><p><strong>Keywords</strong> Remote Sensing . OLI. ALOS PALSAR . ASTER . Kerdous Inlier . Anti Atlas</p>

Geological mapping of carbonatites and related ores from the Oulad Dlim massif (Dakhla Province, Morocco) using remote sensing, portable X-ray fluorescence, and mineralogical data

<p>During the last decades, carbonatites and associated rocks have received increased interest from mining companies and the scientific community. They represent a classic source of a variety of critical elements required by certain emerging technologies and industries such as niobium, rare earth elements (REE), and phosphorus. Morocco like many other countries have several Alkaline igneous complexes, however, their potential in terms of REE-P-rich carbonatites is poorly explored and needs to be investigated. This study is an attempt to develop an advanced exploration tool for the detection and mapping of these rocks using remote sensing.  Preliminary investigations were focused on the Oulad Dlim massif at the western Reguibat Shield (Southeast of Dakhla province) where several carbonatite structures were reported, including Gleibat Lafhouda, Twihinate, Lamlaga, Lahjayra. Advanced Spaceborne Thermal and Reflection Radiometer (ASTER) data were used to: (i) identify and map carbonatites and associated rocks liable to contain REE-P mineralization, (ii) investigate their spectral features, and produce predictive maps. Several image processing techniques, have been performed including band ratio, color composite image, principal component analysis and minimum noise fraction. The combination of these techniques appears to more effectively detect carbonatites and associated rocks. The effectiveness of this approach was verified using field investigation, in-situ geochemical analysis with portable X-ray fluorescence, and petrography. The field data were used to train classifiers to better delineate the spatial distribution of the different lithological facies. The results are generally consistent with available geological maps indicating that this approach can be satisfactorily applied in the early stages of geological exploration.</p>