scholarly journals Antimicrobial activity of copper surfaces against biofilm formation by Salmonella Enteritidis and its potential application in the poultry industry

2021 ◽  
Vol 94 ◽  
pp. 103645
Author(s):  
Karine Patrin Pontin ◽  
Karen Apellanis Borges ◽  
Thales Quedi Furian ◽  
Daiane Carvalho ◽  
Daiane Elisa Wilsmann ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Potential Application ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Poultry Industry ◽  
Copper Surfaces

scholarly journals Antimicrobial Resistance Profiling of Biofilm Forming Non Typhoidal Salmonella enterica Isolates from Poultry and Its Associated Food Products from Pakistan

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 785
Author(s):  
Abubakar Siddique ◽  
Sara Azim ◽  
Amjad Ali ◽  
Saadia Andleeb ◽  
Aitezaz Ahsan ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Multiple Drug Resistance ◽  
Food Products ◽  
Health Concern ◽  
Multiple Drug ◽  
Zoonotic Potential ◽  
Salmonella Spp

Salmonellosis caused by non-typhoidal Salmonellaenterica from poultry products is a major public health concern worldwide. This study aimed at estimating the pathogenicity and antimicrobial resistance in S. enterica isolates obtained from poultry birds and their food products from different areas of Pakistan. In total, 95/370 (25.67%) samples from poultry droppings, organs, eggs, and meat were positive for Salmonella. The isolates were further identified through multiplex PCR (mPCR) as Salmonella Typhimurium 14 (14.7%), Salmonella Enteritidis 12 (12.6%), and other Salmonella spp. 69 (72.6%). The phenotypic virulence properties of 95 Salmonella isolates exhibited swimming and/or swarming motility 95 (100%), DNA degrading activity 93 (97.8%), hemolytic activity 92 (96.8%), lipase activity 87 (91.6%), and protease activity 86 (90.5%). The sopE virulence gene known for conferring zoonotic potential was detected in S. Typhimurium (92.8%), S. Enteritidis (100%), and other Salmonella spp. (69.5%). The isolates were further tested against 23 antibiotics (from 10 different antimicrobial groups) and were found resistant against fifteen to twenty-one antibiotics. All isolates showed multiple drug resistance and were found to exhibit a high multiple antibiotic-resistant (MAR) index of 0.62 to 0.91. The strong biofilm formation at 37 °C reflected their potential adherence to intestinal surfaces. There was a significant correlation between antimicrobial resistance and the biofilm formation potential of isolates. The resistance determinant genes found among the isolated strains were blaTEM-1 (59.3%), blaOxA-1 (18%), blaPSE-1 (9.5%), blaCMY-2 (43%), and ampC (8.3%). The detection of zoonotic potential MDR Salmonella in poultry and its associated food products carrying cephalosporin and quinolone resistance genes presents a major threat to the poultry industry and public health.

scholarly journals Incorporation of Hybrid Nanomaterial in Dental Porcelains: Antimicrobial, Chemical, and Mechanical Properties

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 98
Author(s):  
Carla L. Vidal ◽  
Izabela Ferreira ◽  
Paulo S. Ferreira ◽  
Mariana L. C. Valente ◽  
Ana B. V. Teixeira ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Fracture Toughness ◽  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Silver Ions ◽  
Surface Characteristics ◽  
Streptococcus Sobrinus ◽  
Hybrid Nanomaterial ◽  
Antimicrobial Substances ◽  
Health Area

Biofilm formation on biomaterials is a challenge in the health area. Antimicrobial substances based on nanomaterials have been proposed to solve this problem. The aim was to incorporate nanostructured silver vanadate decorated with silver nanoparticles (β-AgVO3) into dental porcelains (IPS Inline and Ex-3 Noritake), at concentrations of 2.5% and 5%, and evaluate the surface characteristics (by SEM/EDS), antimicrobial activity (against Streptococcus mutans, Streptococcus sobrinus, Aggregatibacter actinomycetemcomitans, and Pseudomonas aeruginosa), silver (Ag+) and vanadium (V4+/V5+) ions release, and mechanical properties (microhardness, roughness, and fracture toughness). The β-AgVO3 incorporation did not alter the porcelain’s components, reduced the S. mutans, S. sobrinus and A. actinomycetemcomitans viability, increased the fracture toughness of IPS Inline, the roughness for all groups, and did not affect the microhardness of the 5% group. Among all groups, IPS Inline 5% released more Ag+, and Ex-3 Noritake 2.5% released more V4+/V5+. It was concluded that the incorporation of β-AgVO3 into dental porcelains promoted antimicrobial activity against S. mutans, S. sobrinus, and A. actinomycetemcomitans (preventing biofilm formation), caused a higher release of vanadium than silver ions, and an adequate mechanical behavior was observed. However, the incorporation of β-AgVO3 did not reduce P. aeruginosa viability and increased the surface roughness of dental porcelains.

Determination of the minimum suppressive concentration of photosensitizers of the second generation in relation to sanitaryindicative microorganisms

2021 ◽  
Vol 1 (11) ◽  
pp. 42-46
Author(s):  
Vadim Yu. Shlenskiy ◽  
◽  
Tatiana I. Pashnik ◽  
Svetlana Yu. Pigina ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Second Generation ◽  
Research Work ◽  
Protoporphyrin Ix ◽  
Bactericidal Effect ◽  
Disodium Salt ◽  
Bacterial Cultures

In the studies described in the article, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed. In the experiments, photosensitizers of the second generation were used: «Photoditazine», the active substance of which is dimeglumin chloride E6, and «Dimegin», which includes a derivative of protoporphyrin IX-2,4-di(1-methoxyethyl)- deuteroporphyrin IX disodium salt. In the described research work, the minimum concentrations of the studied drugs were determined by the method of sequential tenfold dilutions to obtain a bactericidal effect on strains of sanitary-indicative microorganisms Enterococcus faecalis, Staphylococcus aureus, Salmonella enteritidis.

scholarly journals Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2077 ◽  
Author(s):  
Ilioara Oniga ◽  
Cristina Pușcaș ◽  
Radu Silaghi-Dumitrescu ◽  
Neli-Kinga Olah ◽  
Bogdan Sevastre ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Biological Properties ◽  
Gamma Glutamyl Transferase ◽  
Reducing Ability ◽  
Biological Studies ◽  
Antioxidant And Antimicrobial Activity ◽  
Agar Well Diffusion Assay ◽  
New Research ◽  
Glutamyl Transferase

The biological properties and main phenolic compounds of the O. vulgare L. ssp. vulgare extract are described in the present paper. The polyphenolic compounds were analyzed by chromatographic and spectrophotometric techniques. The antioxidant potential was evaluated using several methods: CUPRAC (cupric ion reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), inhibition of lipid peroxidation catalyzed by cytochrome c, and superoxide (SO) scavenging assays. The antimicrobial activity of the oregano extract was evaluated by means of agar-well diffusion assay. The hepatoprotective effect of the O. vulgare extract on CCl4-induced hepatotoxicity was evaluated in rats. Liver injury was estimated by determination of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), gamma-glutamyl transferase GGT, total protein and albumin concentrations, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA). These values were improved by the administration of oregano extract. A specific phenolic profile was evidenced by these data, with large amounts of rosmarinic and chlorogenic acids. The oregano extract showed very strong antioxidant activity in good agreement with the phenolic content. Antimicrobial activity was good, especially against Salmonella enteritidis and Aspergillus niger strains. The high hepatoprotective, antioxidant and antimicrobial activity, along with polyphenol-rich content, can support the use of O. vulgare in therapy. We also expect our results to open new research directions for designing important new drug products, using indigenous plant material.

scholarly journals Microbial Communities of Culture Water and African Catfish Reared in Different Aquaculture Systems in Nigeria Analyzed Using Culture Dependent Techniques

2019 ◽  
pp. 1-18
Author(s):  
D. Enyidi Uchechukwu ◽  
M. T. Okoli
Keyword(s):  
Microbial Communities ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Body Part ◽  
Pond Water ◽  
Klebsiella Pneumonia ◽  
African Catfish ◽  
E Coli ◽  
Earthen Ponds ◽  
Concrete Pond

The microbial communities of culture water and catfish C. gariepinus from three replicates of earthen, concrete and tarpaulin ponds in Nigeria were analyzed. Waters was collected from 25 cm below pond water surface per culture system. Three catfish per replicate system were also collected and analyzed in the lab. Catfish gut, skin and gills were analyzed. Earthen ponds had significantly more diverse microbial community and coliform forming units (CFU/ml) 2.43 x10-4 CFU/ml than the rest systems. Earthen ponds had consortium of Klebsiella pneumonia, S. aureus and Salmonella enteritidis and E. coli, which was more diverse than all other aquaculture systems.  Microbiota of tarpaulin ponds was 2.10x10-4 /ml CFU and this was significantly (P<0.05) higher than concrete ponds (1.50x10-4 CFU/ml). Tarpaulin ponds had K. pneumoniae and E. coli, while concrete pond had S. aureus and S. enteritidis. Biofilm formation could have lead to colonization of the fish body part. The skin and gills had similar microbiota as the culture water compared to the gut. The gut microbial communities were not synonymous with the culture water.

scholarly journals BIOFILM FORMATION OF STAPHYLOCOCCUS AUREUS, SALMONELLA ENTERITIDIS AND PSEUDOMONAS AERUGINOSA IN PRESENCE OF N-BENZOTIAZOL-2-YL-BENZENSULFONAMIDE DERIVATIVES

2010 ◽  
Vol 0 (3(11)) ◽  
pp. 32-40
Author(s):  
I. О. Малярчик ◽  
Т. О. Філіпова ◽  
Т. О. Філіпова ◽  
Б. М. Галкін ◽  
Б. М. Галкін
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Salmonella Enteritidis

scholarly journals Synthesis, spectral characterization and antimicrobial activity of some M(II) complexes with Ciprofloxacin

2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Emir Horozić ◽  
Amira Cipurković ◽  
Zahida Ademović ◽  
Demir Bjelošević ◽  
Amila Zukić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Antimicrobial Screening ◽  
Fluoroquinolone Antibiotics ◽  
Wide Range ◽  
The Difference ◽  
Oxygen Donors ◽  
And Staphylococcus Aureus

Ciprofloxacin, CFL is a drug that belongs to the second generation of fluoroquinolone antibiotics with a wide range of effects on Gram-positive and Gram-negative bacteria. The aim of this work was to investigate the interaction of CFL as ligand with divalent biological cations (Mn2+, Ni2+ and Co2+) in approximate physiological conditions. Synthesized complexes were characterized using FTIR and stereo-microscopy. Antimicrobial screening was performed on bacterial strains of Escherichia coli, Salmonella Enteritidis, Enterococcus faecalis and Staphylococcus aureus. The results of FTIR spectroscopy showed that the M(II) complexes with CFL were formed through the oxygen donors of the carboxyl and carbonyl group of the ligand. Stereo-microscopic characterization revealed the difference in color and size of crystals of the ligand and metal complexes. Antimicrobial screening has shown that CFL and complexes have almost similar antimicrobial activity against investigated bacterial strains.

Antimicrobial activity, inhibition of biofilm formation, and molecular docking study of novel Ag-NHC complexes

2021 ◽  
Vol 954-955 ◽  
pp. 122082
Author(s):  
Neslihan Şahin ◽  
Elvan Üstün ◽  
Uğur Tutar ◽  
Cem Çelik ◽  
Nevin Gürbüz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Docking ◽  
Biofilm Formation ◽  
Docking Study ◽  
Molecular Docking Study ◽  
Activity Inhibition

Antibacterial Mechanism of Vanillic Acid on Physiological, Morphological, and Biofilm Properties of Carbapenem-Resistant Enterobacter hormaechei

2019 ◽  
Vol 83 (4) ◽  
pp. 576-583 ◽  
Author(s):  
WEIDONG QIAN ◽  
MIN YANG ◽  
TING WANG ◽  
ZHAOHUAN SUN ◽  
MIAO LIU ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Natural Products ◽  
Membrane Potential ◽  
Cell Morphology ◽  
Vanillic Acid ◽  
Biofilm Formation ◽  
Diffusion Method ◽  
Intracellular Atp ◽  
Carbapenem Resistant ◽  
Enterobacter Hormaechei

ABSTRACT Many studies have evaluated the antimicrobial activity of natural products against various microorganisms, but to our knowledge there have been no studies of the possible use of natural products for their antimicrobial activity against Enterobacter hormaechei. In this study, we investigated vanillic acid (VA) for its antimicrobial activities and its modes of action against carbapenem-resistant E. hormaechei (CREH). The MIC of VA against CREH was determined by the agar diffusion method. The antibacterial action of VA against CREH was elucidated by measuring variations in intracellular ATP concentration, intracellular pH, membrane potential, and cell morphology. Moreover, the efficacy of VA against biofilm formation and VA damage to CREH cells embedded in biofilms were further explored. Our results show that VA was effective against CREH with a MIC of 0.8 mg/mL. VA could rupture the cell membrane integrity of CREH, as measured by a decrease of intracellular ATP, pH, and membrane potential, along with distinctive alternations in cell morphology. In addition, VA exerted a remarkable inhibitory effect on the biofilm formation of CREH and also killed CREH cells within biofilms. These findings show that VA has a potent antibacterial and antibiofilm activity against CREH and, hence, has the potential to be used clinically as a novel candidate agent to treat CREH infections and in the food industry as a food preservative and surface disinfectant. HIGHLIGHTS

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