Antioxidant potency of gallic acid, methyl gallate and their combinations in sunflower oil triacylglycerols at high temperature

Background: It is important to study about the use of natural antioxidants as alternatives to synthetic ones due to the possibility of carcinogenic effects of synthetic antioxidants. This study is comparing the effect of the ultrasound-assisted and maceration extraction methods on antioxidant activity of Matricaria recutita. Methods: Bioactive compounds including phenolic, tocopherol, flavonoid and tannins and antioxidant activity of the extracts were evaluated. Moreover, extracts obtained from ultrasound and maceration methods were added to sunflower oil without any antioxidants at level of 200, 500 and 800 ppm, after that samples were heated at 180°C. Oxidation of the samples were evaluated after 0, 4, 8, 12, 16, 20 and 24 hours by measuring Peroxide Value (PV), Conjugated Diene (CD), Iodine Value (IV), Carbonyl Value (CV), Total Polar Compounds (TPC), Oil Stability Index (OSI), Color Index (CI) and acid value (AV). Results: The result showed total phenol (42.90 mg gallic acid/g extract), tocopherol (120.46 µg α - tocopherol/ml extract), flavonoid (2.64 mg/100 g extract) and tannins (3.89 mg gallic acid/g extract) of ultrasound extracts were higher than maceration extracts. Antioxidant activity of the extract was evaluated by DPPH assay which indicated 800 ppm of the Matricaria recutita extracted by ultrasound was the highest radical scavenging ability. Conclusion: Result indicated both ultrasound and maceration extracts could increase the oil oxidative stability but could not increase compared to BHA. In most cases, the extract samples by ultrasound had a better effect on stabilizing of sunflower oil during frying.

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CHEMICAL CONSTITUENTS OF Bischofia javanica

Vietnam Journal of Science and Technology ◽

10.15625/0866-708x/55/2/8608 ◽

2017 ◽

Vol 55 (2) ◽

pp. 188

Author(s):

Nguyen Thi Mai

Keyword(s):

Methyl Ester ◽

Gallic Acid ◽

Nmr Spectra ◽

Methanol Extract ◽

Chemical Constituents ◽

Acid Methyl Ester ◽

First Report ◽

Acid Methyl ◽

Bischofia Javanica ◽

Reported Data

From the methanol extract of Bischofia javanica leaves, five compounds including 5'-b-D-glucopyranosyloxyjasmonic acid methyl ester (1), 2-(4-hydroxy-3-methoxyphenyl)ethyl-O-β-D-glucopyranoside (2), hexyl-O-β-D-glucopyranoside (3), friedelan-3-one (4), and gallic acid (5) were isolated. Their structures were elucidated by NMR spectra as well as in comparison with previous reported data. This is the first report of 1 and 2 from Bischofia javanica.

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Quality Characteristics and Standards for Sunflower Oil from Arad Agricultural Area

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:6582 ◽

2011 ◽

Vol 68 (2) ◽

Author(s):

Claudia MUREŞAN ◽

Anca Mihaela DICU ◽

Virgil CIUTINA ◽

Dorina CHAMBRE ◽

Claudiu URSACHI

Keyword(s):

Fatty Acid ◽

High Temperature ◽

Peroxide Value ◽

Sunflower Oil ◽

Quality Characteristics ◽

Conjugated Dienes ◽

Chemical Parameters ◽

Physico Chemical ◽

Specific Absorbance ◽

Conjugated Dienes And Trienes

The purpose of this study was to determine the unsaturated fatty acid compounds of studied oils, physico-chemical parameters, and the behavior of the four oil types during oxidation and heating. This is a part of a large study effectuated on Carnia sunflower hybrids, PR64H91, PR65A22 and PR64H45 obtained through cold pressing. We followed the evolution of the UV absorption correlated with the peroxide value. The peroxide value has been presented an increased value in the first 12 heating hours, but after this period of time, it has been decreased due to the high temperature instability of the early formed hydro-peroxides. The absorbance between 220-230 nm and the specific absorbance on 232 and 270 nm (characteristic of the conjugated dienes and trienes) have increased along the experiment period. The results were compared with those from regular sunflower oil.

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Antibacterial Activity of Bioflavonoid from Fruit Pulps of Acacia nilotica Willd

Microbiology Research Journal International ◽

10.9734/mrji/2019/v28i430139 ◽

2019 ◽

pp. 1-12

Author(s):

O. P. Oladosu ◽

N. R. Isu ◽

I. M. Aboh ◽

S. E. Okhale ◽

A. T. Orishadipe ◽

...

Keyword(s):

Antibacterial Activity ◽

Liquid Chromatography ◽

Gallic Acid ◽

High Performance ◽

Acacia Nilotica ◽

Multi Drug Resistance ◽

Klebsiella Pneumonia ◽

Methyl Gallate ◽

Medicinal Uses ◽

Test Organisms

The emergence of multi-drug resistance in bacteria has led to call for research and development of new leads as antibiotics from medicinal plants. Acacia nilotica (Linn) is a plant of multipurpose medicinal uses, three bioactive flavonoids (methyl gallate, gallic acid and catechin) were isolated from its fruit pulps through a bioassay guided fractionation technique and characterized based on High Performance Liquid Chromatography, Liquid Chromatography-Mass Spectra and Nuclear Magnetic Resonance spectra. Antibacterial activity of these compounds was determined by microplate tetrazolium dye assay of broth microdilution technique against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and clinical isolates of Salmonella typhi, Klebsiella pneumonia, Candida albicans and Bacillus subtilis. Catechin, methyl gallate and gallic acid at 19.5, 39 and 39 µg/ml respectively caused a significant bio-reduction in cells of test organisms. Time kill kinetic study of the extract shows that there was percentage of growth reduction in test organisms at 2, 4, 6, 8 and 12 hrs of contact. The extent and rate of killing of the organism by the extract at 2 x MIC followed the same trend as rate of killing was time dependent. Antibacterial effects of these compounds are within the breakpoint of control drug chloramphenicol and could serve as leads in new drug development.

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Isolation and Purification of Bioactive Compounds from the Stem Bark of Jatropha podagrica

Molecules ◽

10.3390/molecules24050889 ◽

2019 ◽

Vol 24 (5) ◽

pp. 889 ◽

Cited By ~ 4

Author(s):

Truong Minh ◽

Tran Xuan ◽

Hoang-Dung Tran ◽

Truong Van ◽

Yusuf Andriana ◽

...

Keyword(s):

Mass Spectrometry ◽

Ethyl Acetate ◽

Gallic Acid ◽

Bioactive Compounds ◽

Stem Bark ◽

Ionization Mass ◽

Methyl Gallate ◽

Antioxidant Power ◽

Isolation And Purification ◽

Chemical Structures

This paper reports the successive isolation and purification of bioactive compounds from the stem bark of Jatropha podagrica, a widely known medicinal plant. The ethyl acetate extract of the stem bark exhibited the strongest antioxidant activity assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric reducing antioxidant power (FRAP) assays (IC50 = 46.7, 66.0, and 492.6, respectively). By column chromatography (CC) with elution of hexane and ethyl acetate at 8:2, 7:3, and 6:4 ratios, the isolation of this active extract yielded five fractions (C1–C5). Chemical structures of the constituents included in C1–C5 were elucidated by gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) and resolved as methyl gallate (C1, C2, C3, C4), gallic acid (C1, C2), fraxetin (C2, C3, C4, C5), and tomentin (C3). Mixture C2 (IC50 DPPH and ABTS = 2.5 µg/mL) and C3 (IC50 FRAP = 381 µg/mL) showed the highest antioxidant properties. Among the isolated fractions, C4 was the most potential agent in growth inhibition of six bacterial strains including Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Listeria monocytogenes, Bacillus subtilis, and Proteus mirabilis (MIC = 5, 20, 30, 20, 25, and 20 mg/mL, respectively). All identified constituents exerted an inhibitory activity on the growth of Lactuca sativa, of which the mixture C3 performed the maximal inhibition on shoot (IC50 = 49.4 µg/mL) and root (IC50 = 47.1 µg/mL) growth. Findings of this study suggest that gallic acid, methyl gallate, fraxetin, and tomentin isolated from J. podagrica possessed antioxidant, antibacterial, and growth inhibitory potentials.

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Strategies to Improve the Thermo-Oxidative Stability of Sunflower Oil by Exploiting the Antioxidant Potential of Blueberries Processing Byproducts

Molecules ◽

10.3390/molecules25235688 ◽

2020 ◽

Vol 25 (23) ◽

pp. 5688

Author(s):

Cristina-Ramona Metzner Ungureanu ◽

Mariana-Atena Poiana ◽

Ileana Cocan ◽

Andreea Ioana Lupitu ◽

Ersilia Alexa ◽

...

Keyword(s):

High Temperature ◽

Lipid Oxidation ◽

Oxidative Stability ◽

Sunflower Oil ◽

Inhibitory Effect ◽

Butylated Hydroxytoluene ◽

Convective Heating ◽

Total Oxidation ◽

Freeze Dried ◽

The Impact

This research was conducted in order to establish the effectiveness of two freeze-dried extracts obtained from blueberry processing byproducts resulting from juice manufacturing compared to butylated hydroxytoluene (BHT) in delaying the lipid oxidation of sunflower oil subjected to high-temperature convective heating at 180 °C up to 12 h under simulated frying conditions. The fruits were harvested from spontaneous flora of two regions of Romania, Arieseni (Alba County) and Paltinis (Sibiu County) and the blueberry byproducts extracts (BBE) were noted according to the origin place as ABBE and PBBE. The progress of lipid thermo-oxidation was investigated in terms of peroxide value (PV), p-anisidine value (p-AV), the response of TBA-malondialdehyde interactions assessed by thiobarbituric acid (TBA) method, the total oxidation (TOTOX) value and inhibition of oil oxidation (IO). The recorded data highlighted that BBE exhibit a high inhibitory response on lipid thermo-oxidation. The inhibitory effect was concentration-dependent, thus, the degree of lipid oxidation was in reverse related to the BBE dose. The exposure of the oil samples supplemented with 800 ppm BBE (ABBE, PBBE) to a high-temperature heating for 12 h led to a significant decrease of the assessed indices compared to additives-free sunflower oil sample as follows: PV (46%; 45%), p-AV (21%; 17%), TOTOX (27%; 24%), TBA value (25%; 11%). Regarding the impact of the origin on the potential of BBE to inhibit the lipid oxidative degradation, it was noted that ABBE derived from blueberries grown in a region with a milder climate with moderate precipitations and higher temperatures showed a stronger inhibitory effect on lipid thermo-oxidation than PBBE. A moderate level of 500 ppm BBE inhibited the lipid oxidation similar to 200 ppm BHT. The reported results reveal that BBE represent efficient natural antioxidants that could be successfully applied to improve the thermo-oxidative stability of sunflower oil used in various high-temperature food applications.

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Ellagitannins from Geranium potentillaefolium and G. bellum

Natural Product Communications ◽

10.1177/1934578x1000500407 ◽

2010 ◽

Vol 5 (4) ◽

pp. 1934578X1000500

Author(s):

Juan A. Gayosso-De-Lucio ◽

J. Martín Torres-Valencia ◽

Carlos M. Cerda-García-Rojas ◽

Pedro Joseph-Nathan

Keyword(s):

Nmr Spectroscopy ◽

Gallic Acid ◽

Ellagic Acid ◽

Therapeutic Dose ◽

2D Nmr ◽

Published Data ◽

Methyl Gallate ◽

2D Nmr Spectroscopy ◽

Plant Materials ◽

Aerial Parts

The aerial parts of Geranium potentillaefoium afforded geraniin (1), corilagin (2), gallic acid (4), methyl gallate (6), methyl brevifolincarboxylate (7), quercetin, quercetin 3- O-β-D-glucopyranoside, quercetin 3- O-β-D-[6″- O-galloyl)glucopyranoside, kaempferol, β-sitosterol 3- O-β-D-glucopyranoside and β-sitosterol, while the aerial parts of G. bellum gave the same compounds in addition to kaempferol 3- O-β-D-glucopyranoside, isolated instead of kaempferol. The substances were identified by 1D and 2D NMR spectroscopy in comparison with published data. The water decoction preparations from air-dried plant materials (2.5 g) contain ca. 4.6 % of the ellagitannin 1, envisaging that when such decoction is ingested (250 mL), a therapeutic dose of ca. 36 mg of the antitumor ellagic acid (3) may be incorporated into the organism.

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