scholarly journals Protection by dietary Spirulina platensis against d-galactosamine- and acetaminophen-induced liver injuries

2010 ◽  
Vol 103 (11) ◽  
pp. 1573-1576 ◽  
Author(s):  
Jun Lu ◽  
Di-Feng Ren ◽  
Jian-Zhong Wang ◽  
Hiroo Sanada ◽  
Yukari Egashira
Keyword(s):  
Body Weight ◽  
Spirulina Platensis ◽  
Control Diet ◽  
Thiobarbituric Acid ◽  
Positive Control ◽  
Butylated Hydroxytoluene ◽  
Standard Diet ◽  
Tbars Level ◽  
Liver Injuries ◽  
Glutamate Pyruvate

Increasing attention has been paid to Spirulina for its potential clinical uses. The present study investigated the protection by dietary Spirulina platensis against d-galactosamine (d-GalN)- and acetaminophen (APAP)-induced hepatitis in ICR mice. Mice in each group (n 6) were fed with a standard diet (American Institute of Nutrition (AIN)-93G), a positive control diet containing 0·5 % butylated hydroxytoluene (BHT), or a diet containing 3, 6 or 9 % S. platensis for 1 week. On the last day the mice were treated with d-GalN (300 mg/kg body weight, intraperitoneally) or APAP (150 mg/kg body weight, intraperitoneally) and 24 h later the mice were killed. The doses of both 6 and 9 % S. platensis were found to significantly alleviate the increase of serum glutamate oxaloacetoacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities in d-GalN- or APAP-intoxicated mice. The observation was very similar to that of the positive control groups. Two more experiments were carried out to investigate the involvement of thiobarbituric acid-reactive substances (TBARS) and IL-18 in the suppression of 6 % S. platensis on d-GalN- and APAP-induced hepatitis. The significant increase of GOT and GPT activities was found to be accompanied with the elevation of hepatic TBARS level, IL-18 mRNA expression and serum IL-18 concentration, and was significantly alleviated by supplementation with 6 % S. platensis in diets. These results showed that dietary S. platensis could provide a significant protection against d-GalN- and APAP-induced liver injuries, and IL-18 and lipid peroxidation might be involved in the protective influence of S. platensis.

scholarly journals Oxidative stability of tilapia feeds containing Saccharomyces cerevisiae and Spirulina platensis

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Thiago Luís Magnani Grassi ◽  
Juliana Sedlacek-Bassani ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vitamin E ◽  
Spirulina Platensis ◽  
Nile Tilapia ◽  
Room Temperature ◽  
Control Diet ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Rancidity ◽  
Plastic Bags

ABSTRACT: The aim of this study was to evaluate the effect of the inclusion of microbial biomass on the oxidative rancidity of tilapia rations stored for 12 months. Treatments included a control diet and diets supplemented with either 0.01% vitamin E, 0.25 and 0.5% of Saccharomyces cerevisiae and 0.25 and 0.5% of Spirulina platensis. Experimental diets were stored in the dark inside plastic bags at room temperature (25 °C) for 12 months. The oxidative rancidity was measured as thiobarbituric acid reactive substances (TBARS). It was concluded that the inclusions of Spirulina platensis at 0.25% (1.734 ± 0.206) and 0.5% (1.629 ± 0.181) and Saccharomyces cerevisiae at 0.5% (1.459 ± 0.305) minimized the oxidative rancidity in comparation to control diet (2.843 ± 0.109) of Nile tilapia until 12 months of storage.

scholarly journals Control of the lipid oxidation in Nile tilapia feed

2016 ◽  
Vol 46 (9) ◽  
pp. 1675-1677 ◽  
Author(s):  
Thiago Luís Magnani Grassi ◽  
Marcelo Tacconi de Siqueira Marcos ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Lipid Oxidation ◽  
Nile Tilapia ◽  
Control Diet ◽  
Bacterial Biomass ◽  
Thiobarbituric Acid ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Rubrivivax Gelatinosus ◽  
Experimental Treatments

ABSTRACT: The purpose of this research was to investigate the progress of the rancidity in Nile tilapia diets containing bacterial biomass of Rubrivivax gelatinosus . Six experimental treatments comprised basal diet (negative control), diet with asthaxanthin (positive control) and 4 diets with different concentrations of the bacterial biomass. The thiobarbituric acid assay for rancidity analyses were accomplished after 6 and 12 months of diets storage. It was concluded that Rubrivivax gelatinosus biomass minimized the racidity in Nile tilapia diets in 32.52 to 44.72% at 6 months and in 37.85 to 52.37% at 12 months of storage.

scholarly journals A Method to Increase the Viability of Cyprinus Carpio (Linnaeus, 1758) Stocking of the Aquatories Under the Influence Advanced Biotechnologies

2021 ◽  
Vol 21 (4) ◽  
pp. 139-148
Author(s):  
Olena Honcharova ◽  
Pavlo Kutishchev ◽  
Yevgen Korzhov
Keyword(s):  
Body Weight ◽  
Chlorella Vulgaris ◽  
Spirulina Platensis ◽  
Control Diet ◽  
Scientific Work ◽  
Artemia Salina ◽  
The Body ◽  
Biologically Active ◽  
Control Group ◽  
Natural Food

This research investigated Spirulina Platensis, Chlorella vulgaris and Artemia salina as additional sources of protein and biologically active substances that affect the parameters of the carp body. Additional addition of carp to the diet in the ratio: Spirulina Platensis - 40%, Chlorella vulgaris - 30% and Artemia salina - 30% made an impact on the parameters of carp development. The components were cultured in a bioreactor built into a recirculating aquaculture system. The results showed that after additional feeding of carp with the indicated components, the body weight of one-year carp was higher than the control group by 8.9%, and safety by 3.4%. Body weight two year carp was greater in the experimental group than in the group where the fish ate the total diet. The difference was 12.1% in body weight and 2.9% in safety. These parameters were significantly lower in fish fed the control diet (P<0.05). This scientific work has demonstrated the additional use of natural food as a method for improving the blood composition of carp: an increase in hemoglobin by 15.5% (P<0.05), erythrocytes by 5.5% in comparison with the control group. Indicators of gas-discharge visualization of a biological object supplemented the obtained result.

scholarly journals 140 Effects of Bacillus subtilis probiotics on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 79-80
Author(s):  
Yijie He ◽  
Kwangwook Kim ◽  
Cynthia Jinno ◽  
Lauren Kovanda ◽  
Seijoo Yang ◽  
...  
Keyword(s):  
Body Weight ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Control Diet ◽  
Positive Control ◽  
Negative Control ◽  
Enterotoxigenic Escherichia Coli ◽  
Weaned Pigs ◽  
E Coli

Abstract The objective of this experiment was to investigate the effects of Bacillus subtilis on growth performance, diarrhea and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a strain of E. coli (F18, express genes of LT, STb, and SLT 2 toxins). Weaned pigs (n = 48, 6.17 ± 0.36 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four dietary treatments: negative control (NC, control diet without E. coli challenge), positive control (PC, control diet with E. coli challenge), and supplementation of 50 mg/kg of carbadox or 500 mg/kg of Bacillus subtilis probiotics. The experiment lasted 28 d with 7 d before and 21 d after the first E. coli inoculation. The F18 E. coli were given to pigs at 1010 CFU/3 mL dose for three consecutive d. Diarrhea score was daily recorded for each pig to calculate frequency of diarrhea. Fecal samples were collected on d 0, 3, 7, 14, and 21 PI to analyze β-hemolytic coliforms. Data were analyzed using the Mixed Procedure of SAS. Pigs supplemented with carbadox had greater (P < 0.05) body weight on d 7, 14, and 21 PI than pigs in the PC and probiotics group. Supplementation of probiotics enhanced pig body weight on d 21 PI, compared with the PC. E. coli challenge reduced (P < 0.05) ADG and feed efficiency from d 0 to 21 PI, while supplementation of antibiotics or probiotics enhanced ADG and feed efficiency from d 0 to 21 PI. Pigs in carbadox and probiotics groups had reduced (P < 0.05) frequency of diarrhea throughout the experiment and fecal β-hemolytic coliforms on d 7 PI than pigs in the PC. In conclusion, supplementation of Bacillus subtilis could enhance disease resistance and promote growth performance of weaned pigs under disease challenge condition.

scholarly journals Different Doses of Curcumin Supplementation Did Not Improve Biochemical Profile and Weight in C57BL/6 Male Mice Receiving High-Fat Diet

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 699-699
Author(s):  
Caroline Silva ◽  
Priscila Fassini ◽  
Leandra Ramalho ◽  
Daniela Sartori ◽  
Vivian Suen
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Statistical Significance ◽  
Control Diet ◽  
Standard Diet ◽  
Biochemical Profile ◽  
Male Mice ◽  
High Fat ◽  
Pancreatic Steatosis ◽  
Different Doses

Abstract Objectives Curcuma supplementation has been investigated to prevent or treat obesity. However, evidence suggests development of pancreatic steatosis as well. In this context, the aim of this study was to investigate the effect of different doses of curcumin supplementation on weight, biochemical profile, and histological analysis of the pancreas and liver of mice fed a high-fat diet. Methods This was an experimental, longitudinal and randomized study. Fifty C57BL/6 male mice, thirty days age, were separated into five groups: 1. Standard diet (n = 10); 2. High-fat diet (n = 10); 3. High-fat diet plus 50 mg of curcumin/kg of body weight (n = 10); 4. High-fat diet plus 250 mg of curcumin/kg of body weight (n = 10); 5. High-fat diet plus 500 mg of curcumin/kg of body weight (n = 10). Group 1 was fed a standard control diet (AIN 93 G), group 2 was fed a purified high-fat diet (AIN 93 HF 60%) and both groups received only the vehicle (carboxymethyl cellulose - CMC 1%) by gavage. Mice from groups 3, 4 and 5 were fed a purified high-fat diet (AIN 93 HF 60%) plus curcumin at different doses (50, 250 and 500 mg/kg of body weight diluted in 1% CMC) by gavage for twelve weeks. All groups received food and water ad libitum. At the end of the experimental period, we analysed lipid profile, blood glucose, insulin, histology of the pancreas and liver. ANOVA one way and Kruskal-Wallis analysis were performed and a value of P ˂ 0.05 was used to denote statistical significance. Results Curcumin supplementation did not improve weight and biochemical profile. Additionally, histological changes were not observed at any dose of supplementation. Pancreatic or hepatic steatosis was not evidenced in high-fat diet groups and also in the groups who received curcumin, suggesting no toxic effects at the different doses of supplementation provided. Conclusions Our results suggest that curcumin supplementation has no beneficial effect on weight gain prevention and biochemical profile, regardless of the dose administered. Funding Sources FAPESP (São Paulo Research Foundation).

scholarly journals Effect Of Roselle Petal Extract On Decreased Levels Of Mda In Rats With Type 2 Diabetes

2021 ◽  
Vol 14 (1) ◽  
pp. 48-52
Author(s):  
Novera Herdiani ◽  
Edza Aria Wikurendra
Keyword(s):  
Type 2 Diabetes ◽  
Body Weight ◽  
Thiobarbituric Acid ◽  
Positive Control ◽  
Control Group ◽  
Free Radical Damage ◽  
Peripheral Tissues ◽  
Treatment Dose ◽  
Assay Result

Introduction: Diabetes mellitus type 2 is a metabolic disorder, namely a decrease in peripheral tissues' response to insulin. Free radicals increase, which results in the forming of Malondialdehyde (MDA). Roselle flower petals is an antioxidant to reduce free radical damage. Purpose: This study aims to analyze the effect of roselle petal extract on decreased MDA levels in mice induced by diabetes. Method: The rats were divided into four treatments group as follow: 1) control negative, 2) control positive, 3) treatment dose I (administering roselle petal extract 195 mg/200 gram body weight), 4) treatment dose II (giving roselle petal extract 260mg/ 200 gram body weight). MDA levels were measured using Thiobarbituric acid (TBA) assay. Result: There was an effect of rosella petal extract in the group with 260 mg/200 of rosella petal extract (p=0.005) and 195 mg/200 of rosella petal extract (p=0.041) compared with the positive control group. Conclusion: rosella petal extract significantly reduces MDA levels in rats induced by type 2 diabetes.

scholarly journals The effect of purslane hydroextract and zinc on performance, antioxidant capacity and immunity of broiler chickens during summer conditions

2018 ◽  
Vol 61 (3) ◽  
pp. 365-371
Author(s):  
Rojin Kazemi ◽  
Ardashir Sheikhahmadi ◽  
Ghorbanali Sadeghi
Keyword(s):  
Body Weight ◽  
Ambient Temperature ◽  
Antioxidant Capacity ◽  
Broiler Chickens ◽  
Control Diet ◽  
Basal Diet ◽  
The Body ◽  
Activity Level ◽  
Butylated Hydroxytoluene ◽  
Lactate Dehydrogenase Activity

Abstract. Due to negative effects of high ambient temperature, the present study was conducted to investigate the effects of Portulaca oleracea hydroextract (PHE) and zinc (Zn) on the growth performance and immunity of broiler chickens reared under high summer ambient temperature conditions. A total of 420 day-old male broiler chickens (Ross 308) were randomly allocated to seven dietary treatments each replicated four times with 15 birds per replicate. The basal diet as the control diet was supplemented with 100 mg kg−1 butylated hydroxytoluene (BHT), or 300 and 600 mg kg−1 of PHE, 100 mg kg−1 of zinc and 100 mg kg−1 of zinc plus 300 or 600 mg kg−1 of PHE (ZP300 and ZP600). The result of the present study showed that Zn, PHE and their combination did not alter daily feed intake and feed conversion ratio. Although there was a tendency toward an increase in broiler body weight at 24 and 44 days of age (P=0.059 and P=0.061), the body weight of birds fed the basal diet supplemented with Zn reduced at day 10. The birds fed diets supplemented with BHT or ZP600 showed lower aspartate aminotransferase (ASAT) and lactate dehydrogenase activity at 24 days of age (P<0.05), but it remains lower in the ZP600 group only for ASAT activity level at day 44. The results of the present study suggest that dietary PHE and Zn supplementation partially improved body weight and serum antioxidant capacity, superoxide dismutase activity and antibody titer against sheep red blood cells and Newcastle diseases in the broiler chickens during summer conditions.

Pharmacological investigations on efficacy of Phlorizin a sodium-glucose co-transporter (SGLT) inhibitor in mouse model of intracerebroventricular streptozotocin induced dementia of AD type

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Reena Rani ◽  
Amit Kumar ◽  
Amteshwar Singh Jaggi ◽  
Nirmal Singh
Keyword(s):  
Ache Activity ◽  
Thiobarbituric Acid ◽  
Positive Control ◽  
Control Treatment ◽  
Histopathological Study ◽  
Tbars Level ◽  
Mice Model ◽  
Biochemical Alterations ◽  
Dementia Of Alzheimer’S Type ◽  
Antiinflammatory Effects

Abstract Objectives The study has been commenced to discover the potential of Phlorizin (dual SGLT inhibitor) in streptozotocin induced dementia of Alzheimer’s disease (AD) type. Material and methods Injection of Streptozotocin (STZ) was given via i.c.v. route (3 mg/kg) to induce dementia of Alzheimer’s type. In these animals learning and memory was evaluated using Morris water maze (MWM) test. Glutathione (GSH) and thiobarbituric acid reactive species (TBARS) level was quantified to evaluate the oxidative stress; cholinergic activity of brain was estimated in term of acetylcholinesterase (AChE) activity; and the levels of myeloperoxidase (MPO) were measured as inflammation marker. Results The mice model had decreased performance in MWM, representing impairment of cognitive functions. Biochemical evaluation showed rise in TBARS level, MPO and AChE activity, and fall in GSH level. The histopathological study revealed severe infiltration of neutrophils. In the study, Phlorizin/Donepezil (serving as positive control) treatment mitigate streptozotocin induced cognitive decline, histopathological changes and biochemical alterations. Conclusions The results suggest that Phlorizin decreased cognitive function via its anticholinesterase, antioxidative, antiinflammatory effects and probably through SGLT inhibitory action. It can be conferred that SGLTs can be an encouraging target for the treatment of dementia of AD.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

scholarly journals Fishmeal Replacement with Hermetia illucens Meal in Aquafeeds: Effects on Zebrafish Growth Performances, Intestinal Morphometry, and Enzymology

Fishes ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 28
Author(s):  
Baldassare Fronte ◽  
Rosario Licitra ◽  
Carlo Bibbiani ◽  
Lucia Casini ◽  
Mahanama De Zoysa ◽  
...  
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Control Diet ◽  
Low Carbon ◽  
Replacement Rate ◽  
Hermetia Illucens ◽  
Fishmeal Replacement ◽  
Fish Intestine ◽  
Growth Performances ◽  
Intestinal Morphometry

Fishmeal (FM) is still the most important protein source in aquafeeds. However, due to the reduction of wild fish stocks used for FM production, its manufacturing it is now unsustainable. Insect meal represents a valid alternative to FM, due to the low carbon footprint of its production and its high nutritional value. The aim of this study was to investigate the potentials of replacing FM with black soldier fly (Hermetia illucens) meal (HIM) in aquafeeds, using zebrafish as the animal model. Four diets were formulated with increasing HIM/FM replacement rate (the control diet contained 20% FM). Mortality rates, growth performances, and feed consumptions were measured, and fish intestine samples were collected for histological and enzymatic analysis. After 49 days, all groups almost tripled their initial body weight (cumulative body weight gain ranged between 143.9 and 155.2 mg), and no statistically significant differences among treatments were observed in relation to growth performances and histological traits. Contrarily, trypsin, alkaline phosphatases, and alpha amylase–glucoamylase activities were significantly reduced when the FM replacement rate was increased. In conclusion, HIM may represent an alternative to FM, since no adverse effects were observed when it was included up to 20% in the zebrafish diet, even when replacing 100% of the FM.

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